곤충병원성 곰팡이 Zoophthora radicans (Entomophthorales: Entomophthoraceae)가 혹명나방, 배추좀나방, 복숭아혹진딧물과 진딧물 인 미분류 1종을 감염시키는 것을 발견하였다. 이들은 전라북도 진안, 김제, 익산에서 수집되었고, 6월과 9월에 관찰이 가능하였다. 이 균에 감염 된 혹명나방은 진안에서 수집되었고, 감염율이 46%에 달하였다. 감염된 곤충 충체로부터 균을 분리하여 Sabouraud dextrose agar with yeast extract (SDAY)를 이용하여 인공배양을 시도하였다. 그 결과, 20~28°C가 인공배양을 위한 최적온도로 판단되며, SDAY보다 SDAY supplemented with egg yolk and milk (SDAY-EM)에서 발육이 보다 양호하였다. 이 곰팡이는 자연적으로 발생함을 포장에서 확인할 수 있었다.

MicroRNAs are ~22nt small noncoding RNAs that control gene expression at the posttranscriptional level through translational inhibition and destabilization of their target mRNAs. Micro RNAs are phylogenetically conserved and have been shown to be instrumental in a wide variety of key biological processes including cell cycle regulation, apoptosis, control of metabolic pathways, imprinting and differentiation. The expression of miRNAs is often regulated in tissue specific and developmental stage‐specific manners. More than 500 miRNAs have been reported in diverse eukaryotic organism so far. One of the biological functions of miRNAs seems to be the regulation of self‐renewal versus differentiation in stem cells. Recent efforts have focused on defining the miRNA expression profile in undifferentiated ESCs as compared to their differentiated progeny. Among the so‐called ES‐specific miRNAs, the 302‐367 cluster stands out due to its intracellular abundance and high cell type specificity. Levels of miRNA 302‐367 correlate with Oct4 transcripts in ESCs and early embryonic development, indicating an important role in ESC homeostasis and maintenance of pluripotency. Several months ago, a paper showed that expression of the miRNA 302‐367 cluster can directly reprogram mouse and human somatic cell to an iPS cell in absence of any of the four factors (Oct4, Sox2, c‐Myc, Klf4) efficiently. To apply this efficient method to porcine, we made an inducible vector system including miRNA 302‐367 cluster originated from porcine embryonic fibroblasts and could make porcine ips by the miRNA 302‐367 cluster.