When kiwifruit root system infected with nematodes was treated by hot water dipping treatment, the maximum temperature for this treatment was suggested as 50℃. The lowest killing temperature of internal root-knot in the root tissue was 48℃. Consequently, root-knot nematodes could be killed without damaging root tissues by the hot water dipping treatment at 48℃ for 10 minutes. This could be useful for organic production and distribution of kiwifruit seedlings by avoiding the synthetic nematicides which are not easily decomposed in soil once applied.