The purpose of this study is to select various insect species for healing resources and develop a healing program in order to use insect as a healing agriculture. In this study, there are two kinds of breeding kit were developed, one for Gryllus bimaculatus and the other for Oxya chinensis sinuosa. Using these insect breeding kits, we conducted a survey of 60 children and the elderly. In the case of children, the results of the insect breeding satisfaction showed that 30.6% said that the sound of crickets was very good, and 11.1% said that it was good. In addition, the higher the child's awareness of insects, the higher the proportion of children who wanted to raise insects in the future. As a result of a survey of seniors, 45.2% do not like insects and 51.6% are not interested, meaning that most seniors are not very interested in insects. However, the emotions after breeding insects showed positive results, with 45.2% saying their personality became brighter, 48.4% reducing their anger, 48.4% relieving their irritation, 54.8% relieving loneliness, 58.1% feeling more responsible, and 51.6% developing intimacy.

갈색거저리 유충의 사료인 밀기울은 대부분 수입에 의존하고 있는데 일부 국가의 식량 수출 중단 조치 등에 따른 국제 곡물가격 상승으로 밀기울 가격은 인상되고, 식용곤충 판매가격이 하락하면서 생산비 절감을 위한 사료 개발이 요구되고 있다. 농업부산물 3종을 50% 이상 급이하게 되면 유충 생육이 저하되었기 때문에 본 연구는 적정한 배합비율을 선정하기 위해 첨가사료 20, 30% 함량으로 밀기울과 혼합하여 사료를 급이하였을 때 갈색거 저리 유충의 생육 특성과 영양성분 변화에 대해 밀기울만 제공한 대조구와 비교하였다. 부산물 A와 B, C를 각각 30% 함유한 처리구에서 갈색거저리 유충 무게는 대조구와 차이가 없음을 확인하였다. 먹이소화율은 부산물B를 20% 함유한 처리구가 80.5%로 대조구에 비해 높았고, 부산물C 30% 처리구에서 72.6%로 가장 낮았다. 갈색거저 리 유충의 생육일수 100일 기준으로 부산물B 30% 처리구에서 용화율이 76.1%로 대조구보다 1.6배 높았으며, 부산물A 20% 처리구는 29.2%로 용화율이 가장 낮았다. 갈색거저리 유충의 조단백질 함량은 부산물 C 30% 처리 구에서 대조구보다 10.3% 증가하여 아미노산 분석을 진행한 결과 sarcosine과 ornithine이 2.5배 이상 증가하였다. 이를 통해 부산물 B나 C를 30% 함유한 사료를 급이하였을 때 사육 원가를 절감하여 유충을 생산할 수 있을 것으로 사료된다.

The bacterial soft-rot disease is one of the most critical diseases in vegetables such as Chinese cabbage. The researchers isolated two bacteria (Pseudomonas kribbensis and Pantoea vagans) from diseased tissue samples of Chinese cabbages and confirmed them as being the strains that cause soft-rot disease. Lactic-acid bacteria (LAB), were screened and used to control soft-rot disease bacteria. The researchers tested the treatments with hypochlorous acid water (HAW) and LAB supernatant to control soft-rot disease bacteria. The tests confirmed that treatments with the HAW (over 120 ppm) or LAB (Lactobacillus plantarum PL203) culture supernatants (0.5 mL) completely controlled both P. kribbensis and P. vagans.

본 연구에서는 국내산 돌미나리에서 GABA생성능이 있 는 신규 유산균을 분리, 동정한 결과, Enteroccoccus casseliflavus로 확인되었다. 최근까지 Lactobacillus속과 같 은 GABA 생성 유산균에 대한 보고는 많이 되고 있고, 일 부 Enterococcs속 유산균도 보고되고 있으나 E. casseliflavus 종에 대한 보고는 없었다. 따라서 본 연구에서는 E. casseliflavus PL05 균주에 대한 GABA 생성 최적 조건을 찾기 위하여 배지의 유형, 생육 온도, 초기 pH 조건, 배양 시간, MGS 농도 및 탄소원을 포함한 다양한 조건을 테스 트하였다. PL05 균주는 MRS 혹은 TSB 배지보다 BHI 배 지에서 생육이 잘 되었으며, 배지의 초기 pH는 7-9 조건 에서 가장 생육이 왕성하였고, GABA 생성 조건 역시 유 사한 결과로 확인되었다. GABA의 기질에 해당하는 MSG 의 농도별 GABA 생성량을 조사한 결과, 7%에서 가장 높 은 생성량을 나타내었으나 5%에서도 유사한 수준으로 확 인되어 효율적인 측면에서 5%가 적합할 것으로 판단된다. 탄소원에 따른 생육 및 GABA 생성량은 말토오스를 사용 하였을 때 가장 높은 것으로 확인되었고, 이러한 최적의 조건들로 최종 테스트를 진행한 결과, 24시간째 140.06±0.71 mM의 GABA가 생성되었고, 전환율은 78.95%로 확인되 었다. 또한 반코마이신을 포함한 10개의 항생제에 대한 감 수성을 조사한 결과 내성이 없는 것을 확인하였다.

국제적으로 신품종과 품종 육성자의 권리가 지적 재산권으로 보호됨에 따라 화훼작물의 경우 국내 민간종묘에서 품종 개발이 거의 이루어지지 않아 농촌진흥청 원예시험장(구 국립 원예특작과학원) 화훼과에서 1992년부터 본격적으로 품종육성 연구가 시작되었다. 2017년 재배면적 44ha, 판매액 93.6억원 인 주요 난류의 하나인 심비디움은 다른 화종에 비해 육종년 한이 길어 2002년에 ‘뷰티프린세스’ 등 4품종이 육성된 후 2017년까지 51품종이 육성되었고, 농촌진흥청 연구비 지원으로 지산영농조합법인에서도 ‘핑크레이스’ 등 26품종이 육성된 바 있다. 이렇게 개발된 품종은 농가에 보급되어 분화나 절화 로 유통되고 있어 최근에는 국산 품종에 대한 재배농가의 인지도가 높아지고 있다. 2009년부터 2017년까지 aT화훼공판장 에서 거래되고 있는 국산 심비디움 품종들의 분화 거래내역을 조사한 결과, 농촌진흥청 개발 51품종 중 21품종이 지산영농 조합 개발 26품종 중 7품종이 1년에서 5년동안 거래된 것을 확인하였다. 국산 품종 거래 수는 2010년과 2015년을 제외하고는 2,568~5,693분으로 총 심비디움 판매량의 1.5~2.1%를 차지하였다. 심비디움 거래량의 65.2~97.6%를 차지하는 꽃대 2, 3, 4대 가격을 비교한 결과, 국산 품종이 외국산 품종에 비하 여 최고가는 낮았지만 평균가는 높았고, 최저가는 훨씬 높았다. 결론적으로 aT화훼공판장 거래가를 기준으로 국산 품종이 외국산 품종과 비교할 때 품질이 양호하였음을 알 수 있었다.

1913년부터 2013년까지 한국에서 육성된 콩 172품종의 생 육 및 수량구성형질의 변이를 평가하고 주성분 분석과 군집 분석을 통하여 품종군을 분류하여 콩 육종의 기초자료로 이용 코자 수행하였다. 생육 및 수량구성형질의 변이계수는 각 형 질에 따라 차이가 있었으며, 엽장폭비, 경장, 분지수, 100립중 및 주당종실중은 높았고, 엽면적, 주경절수, 주당협수 및 주당 립수는 중간정도이었으며, 생육시기에 관련된 개화일수, 성숙 일수 및 생육일수는 낮았다. 콩 품종들의 생육 및 수량구성형 질에 대한 주성분 분석을 한 결과, 주성분의 기여율은 제 1주 성분은 34.02%, 제 2주성분은 18.44%, 제 3주성분은 10.67%, 제 4주성분은 9.96%로, 상위 4주성분까지의 고유값이 1이상 이며, 누적기여율이 73.09%로 제 4주성분까지만 가지고도 한 국 콩 품종의 군집분류가 가능하였다. Average linkage cluster 에 의하여 분류된 군집분류에서 평균 거리를 1.1로 하였을 때 5개 군집으로 분류되었고, I군집은 전체 품종의 69.7%가 속해 있는 가장 큰 군집이었고, 다음으로 II군집이 19.8%가 속하는 큰 군집이었고, 그 다음으로 IV군집이 8.7%가 속하였으며, III 군집과 V군집은 1 ~ 2품종이 속하는 소군집이었다. 각 군집의 품종을 육성년대(1980년 이전, 1980년대, 1990년대, 2000년 이후) 및 용도별(장류 및 두부용, 나물용, 밥밑용, 풋콩 및 올 콩용)로 분류하면 품종의 분포가 차이가 있었다. 육성년대별로 는 1980년 이전과 1980년대에 육성된 품종은 I과 II군집에, 1990년대와 2000년 이후에 육성된 품종은 모든 군집에 넓게 분포하였다. 용도별로는 I군집에는 장류 및 두부용 품종이, II 군집에는 나물용 품종이, IV군집에는 풋콩 및 올콩용 품종이 가장 많이 속하였으며, III과 V군집에 속하는 품종은 나물용 품종이었다.

The global marketplace is changing and retailers must decide on unique positioning strategies to attract consumers away from the competition. Socioeconomic changes are shifting consumer shopping patterns. Technology development drastically affected retail environment and consumers shopping behavior. While traditional retailers provide consumers to touch and feel merchandise and provide instant gratification, online retailers, meanwhile impress shoppers with wide selection, low prices and other consumers ratings and reviews. As the retailing industry move toward an omnichannel retailing experience, there are no exact distinction between traditional offline and online stores. Under the circumstances, retailers pursue an integrated sales experience that melds the advantages of physical stores with the information-rich experience of online shopping. In order to develop effective marketing strategy, retailers need adapting best practices from both the offline and online worlds in areas including developing products, pricing, designing the shopping experience and building relationships with customers (Brynjolfsson, Hu & Rahman, 2013). In order to do this, the first step is to understand the consumers who DO shopping in stores and “redesign shopping from scratch (Rigby, 2011): Why do they visit the stores? What do they expect from the stores? What does make their shopping experience special? What does shopping mean to consumers? The purpose of this study was to gain a better understanding and conceptualizing of consumer’s perspective on shopping at this era of omnichannel retailing. Given the exploratory nature of the research, a qualitative research methodology was necessary because it is useful in gaining insights from consumers’ own perspectives (Denzin and Lincoln, 1994). The photo elicitation interview method was used for this research. This method allows the research discussion to start with real places and real experiences and visual data are useful for envisioning and speaking about possible, desired futures (Dumreucher & Kolb, 2008). For this research, we invite 20 college students to answer a research question by taking photos and explaining their photos to the researcher in the interview session. We used Dempsey and Tucker (1994)’s five step protocol for photo-interviewing. The five steps include: sourcing photographs, selecting specific photographs, preparing the interview schedule, conducting the interviews, and analyzing textual data. In order to collect sourcing photographs, we asked participants to consider the general research questions (e.g., what does shopping mean to you? What does come across your mind when you read “shopping”?) and how to take photos that reflect their view point on the research questions. Then, participants implemented their reflections by taking 30 photos of specific subjects in their surroundings (e.g., people, places, activities, or buildings) that related to the research questions and are meaningful to them during the next 2 weeks. In the selecting photographs step, photos were grouped in categories. In the interview, participants consider their photos and verbalize their thinking. In the final step, the data—photos, and interview transcripts-were analyzed (Dempsey & Tucker, 1994; Kolb, 2008) and core categories have emerged which have then been refined. The findings suggest that Korean young consumer’s concept of shopping were sorted into six categories: 1) considering various channels to shop (e.g., department store, outlet store, online store, mobile stores, offshore direct buying), 2) thinking of fashion items (e.g., clothing, shoes, cosmetics, accessories), 3) searching for promotional activities (e.g., sales, markdowns, special events, online coupons), 4) browsing and comparing attributes through online stores, 5) releasing stress/changing mood, 6) socializing with others, and 7) getting stress from shopping. Gaining a better understanding of the smart shopper will enable retailers to more accurately target this consumer group. Although various retail channels were the most photographed and mentioned in this study, department stores and mobile stores (using smartphone) were the most mentioned and photographed. Participants also mentioned browsing and comparison shopping through online stores as “pre-shopping activity.” Another area is hedonic aspect of shopping: one of the major meanings of shopping found in this study is releasing stress or changing mood. Some also mentioned that although they do shopping in order to release their stress, sometimes shopping leads to another type of stress. There are also few participants who suggested shopping is a stressful work to do. By the photo elicitation interview, this study illustrates that shopping embrace a multi-dimension of meanings and provides insights to improve our understanding of young Korean consumers’ concept of shopping. The photograph aided in the visual and emotional memory of the experience as a memory anchor as they recalled the places or moments, and using photographs in the research enabled the research to proceed to a deeper understanding and meaning

The centipede Scolopendra subspinipes mutilans has been a medically important arthropod species by using it as a traditional medicine for the treatment of various diseases. In this study, we derived a novel lactoferricin B like peptide (LBLP) from the whole bodies of adult centipedes, S. s. mutilans, and investigated the antifungal effect of LBLP. LBLP exerted an antifungal and fungicidal activity without hemolysis. To investigate the antifungal mechanism of LBLP, a membrane study with propidium iodide was first conducted against Candida albicans. The result showed that LBLP caused fungal membrane permeabilization. The assays of the three dimensional flow cytometric contour plot and membrane potential further showed cell shrinkage and membrane depolarization by the membrane damage. Finally, we confirmed the membrane-active mechanism of LBLP by synthesizing model membranes, calcein and FITC-dextran loaded large unilamellar vesicles. These results showed that the antifungal effect of LBLP on membrane was due to the formation of pores with radii between 0.74 nm and 1.4 nm. In conclusion, this study suggests that LBLP exerts a potent antifungal activity by pore formation in the membrane, eventually leading to fungal cell death.

Actias artemis is a members of the family Saturniidae, also known as wild silkmoths, have impressive color and size. In 2012, estimation of Actias artemis (Lepidoptera: Saturniidae) abundance in HECRI was conducted using the mark-release-recapture (MRR) method (Jolly, 1965) from mid to late May. Seven sampling events were accomplished from 19 May, 21 May, 22 May, 24 May, 26 May, 28 May and on 30 May, during the main flight of the species. Marking was made by writing numbers in the hind wing of each individual moths. Most collections were undertaken by a team of experienced four or six researches of HECRI using light trap (mercury lamp: 250W). Seven female and 58 male moths were captured in study site. The effective population size of Actias artemis was 24.9 and heterozygosity was more than 97%. Seven marked moths were recaptured, resulting in 9.7% of recapture rate. The estimated population size of A. artemis showed a peak by 133 individuals on 22 May and then declined. The estimated adult numbers of A. artemis using MRR method from minimum 168 to maximum 5,332 (p<0.05).

The purpose of this study is to examine the effects of gait training using functional electrical stimulation on the improvement of hemiplegic patients' functions for balance and gait velocity. The subjects of the experiment were determined to be 10 each hemiplegic patients who had been diagnosed with stroke or brain damage six months or longer earlier assigned to an experimental group and a control group respectively. The subjects were evaluated before the experiment using Tetrax and 10M gait tests, received gait training five times a week for four weeks using functional electrical stimulation and were evaluated after the experiment in the same method as used in the evaluation before the experiment. In order to examine differences between the experimental group that received gait training using functional electrical stimulation and the control group that was treated by functional electrical stimulation and received gait training thereafter, differences between before and after the experiment were analyzed using paired sample t-tests and differences in changes after the experiment between the experimental group and the control group were analyzed using independent sample t-tests in order to compare the two groups with each other. Experimental results showed significant differences in weight bearing, balance and gait velocity between before and after the experiment in the experimental group(p<.05). In the control group, whereas weight bearing and gait velocity did not show any significant difference between before and after the experiment(p>.05), balance showed significant differences(p<.05). Weight bearing, balance and gait velocity change rates showed significant differences between the experimental group and the control group(p<.05). In conclusion, it was indicated that gait training using functional electrical stimulation is effective for enhancing stroke patients' weight bearing rates, balance abilities and gait velocity.

캘러스, 반수체, picloram, 전처리양식을 규명할 목적으로 배양효율에 미치는 화분발육 단계와 온도전처리가 미치는 영향에 대한 실험결과는 다음과 같다. ‘Dreamland’의 감수분열중인 약을 현 미경적방법으로 검경하여 본 결과 화뢰의 길이가 23.0-24.9mm는 tetrad 단계이었고 25.0-26.9mm는 uninucleate 단계, 27.0-28.9mm에서는 binucleate 단계 임을 확인할 수 있었으며, 저온 및 고온 온도처리 결 과는 전체적으로 캘러스 및 배 발생율은 저조하였고 특 이한 점은 발견할 수 없었다. Microgametogenesis 단 계별로 약배양 효율을 조사한 결과, late uninucleateearly binucleate 단계인 23.0-28.0mm에서 17.8% callus가 유도되었고, 식물체 재분화율도 6.7%로 나타 나 적정 배양 stage임을 확인할 수 있었다. 유기된 캘 루스를 picloram과 zeatin을 혼용한 MS 배지에 이식 하여 25oC에서 배양하여 식물체를 얻었다.