The effects of lactic acid bacteria (LAB) mixtures on low moisture Italian ryegrass (IRG) silage fermentation was evaluated in field conditions. The experiment was categorized into two groups: Un-inoculated (Control) and Inoculated with LAB mixture for four storage periods (45, 90, 180, and 365 days, respectively). Silage inoculated with the LAB mixture had the lowest pH with highest lactic acid production than the control from beginning at 45-365 days at all moistures. Higher LAB counts were observed in inoculated silages than the control silages at whole experimental periods. It is a key reason for the rapid acidification and higher lactic acid production in silages during the storage periods. Overall results suggest that an adding of LAB mixture had positive effects on the increasing aerobic stability of silage and preserved its quality for an extended duration.

This study was carried out to explore the accuracy of near infrared spectroscopy(NIRS) for the prediction of moisture content and chemical parameters on winter annual forage crops. A population of 2454 winter annual forages representing a wide range in chemical parameters was used in this study. Samples of forage were scanned at 1nm intervals over the wavelength range 680-2500nm and the optical data was recorded as log 1/Reflectance(log 1/R), which scanned in intact fresh condition. The spectral data were regressed against a range of chemical parameters using partial least squares(PLS) multivariate analysis in conjunction with spectral math treatments to reduced the effect of extraneous noise. The optimum calibrations were selected based on the highest coefficients of determination in cross validation(R2) and the lowest standard error of cross-validation(SECV). The results of this study showed that NIRS calibration model to predict the moisture contents and chemical parameters had very high degree of accuracy except for barely. The R2 and SECV for integrated winter annual forages calibration were 0.99(SECV 1.59%) for moisture, 0.89(SECV 1.15%) for acid detergent fiber, 0.86(SECV 1.43%) for neutral detergent fiber, 0.93(SECV 0.61%) for crude protein, 0.90(SECV 0.45%) for crude ash, and 0.82(SECV 3.76%) for relative feed value on a dry matter(%), respectively. Results of this experiment showed the possibility of NIRS method to predict the moisture and chemical composition of winter annual forage for routine analysis method to evaluate the feed value.

This study was conducted to investigate the effect of supplementary feeding levels on livestck and forage productivity and grazing intensity in Elk stags (Cervus canadensis). A fifteen 2-year-old Elk stags about 195 kg were randomly assigned to one of three dietary treatments (five animals per treatment). The dietary treatments consisted of a feeding concentrate of 1.0% of body weight (T1), 1.5% of body weight (T2) and 2.0% of body weight. Total dry matter intake (TDMI) was increased with increased with an increasing supplementary feeding levels. Average daily gain (ADG) were significantly increased with an increasing supplementary feeding levels (p<0.05) and reached a maximum on July and was lower in spring than autumn. The velvet antler production was no differences among treatment groups. Forage productivity of pasture and crude protein content were highest on May and decreased thereafter, however, crude fiber content was the reversed. The grazing intensity of Elk stags was increased in spring (38 to 59 head per ha) than summer and autumn (13 to 32 head per ha). The average grazing intensity of Elk stags ranged from 21 to 34 head per ha, which is affected by supplementary feeding levels. This result suggests that feeding supplementary diet at 1.5 % of body weight was needed to maintain the stable wight gain in antler growing periods and control the proper grazing intensity of Elk deer stags.

This experiment was carried out to study the change of productivity and feed value in different types of hilly pasture. The pasture utilized in the experiment was placed on the experimental farm of Pyeongchang Campus of Seoul National University. Forage production type(FP; Orchardgrass 18 + Tall Fescue 12 + Timothy 5 + White clover 5 kg/ha) and Public farm type(PF: Orchardgrass 12 + Tall Fescue 18 + Timothy 5 + White clover 5 kg/ha) pasture were established in September 3, 2014 and utilized (cutting or grazing) four times every year. Growth characteristics, yield and forage quality were investigated for two years. Plant height of grasses was the highest in the 1st cutting and legumes was in the 2nd cutting. Dry matter (DM) content was highest at every the 1st cut grasses significantly lower at the 2nd harvest (p<0.05). However, there was no significant difference in the 2nd, 3rd and 4th harvest in 2016. In the botanical composition change, the portion of legume was gradually increased after pasture establishment and the ratio of weed and bare land was higher at 2nd and 3rd cutting, but it was decreased at 4th harvest. There was no significant difference in 2016 of fresh yield between two farm types (p>0.05). The yield of dry matter showed similar trend of fresh yield and forage production type was higher than that of public farm type (p<0.05). The forage intake by livestock was 1,452 kg/ha in 2015 and 1,743 kg/ha in 2016. Pasture utilization ratio of public farm type pasture was highest in the 3rd grazing time. Forage quality of pasture in relation to management type had not significant difference, but there was difference in harvest times. Crude protein (CP) was the lowest in the 1st harvest and total digestible nutrient (TDN) was highest in the 1st harvest and lowest in the 4th harvest. Based on the above results, it is found that the establishment of pasture suitable for farm’s situation is important for set up of Korean model of hilly pasture. Although the forage production type is superior on forage productivity, it is recommended that the results will be provided as basic data for management of public farm type in the future.

동계작물인 보리를 재배한 후 하계작물인 수수, 기장 및 피를 대상으로 하여 조사료 생산량과 그에 따른 사료가치를 분석하여 보리와 적합한 하계작물의 최적의 작물조합을 선정하기 위하여 실시한 결과 다음과 같다. 1. 초장은 하계작물 모두 1차 및 2차 수확할 때보다 호숙기가 가장 컸으며, 수확시기에 따른 수분 함량은 1차 수확할 때 가장 높았고 호숙기에 수확할 때 가장 낮은 함량을 보였다. 2. 보리의 건물수량은 10 a 당 1,343 kg였으며 보리와 하계 작물를 작부체계에 따른 건물수량은 수수의 경우 1차 및 2차 수확보다 호숙기에 수확할 때 10,018 kg으로 가장 많았으며, 기장과 피 역시 출수기와 재생 후 수확 시 보다 호숙기에 수확할 때 건물수량이 더 많았다. 3. ADF 함량은 수수는 1차 및 2차 수확 시 보다 호숙기 때가 낮았지만 기장과 피는 호숙기가 더 높은 ADF 함량을 보였다. NDF 함량은 3작물 모두 1차 및 2차 수확 시 보다 호숙기에 더 많은 함량을 보였으며, 조단백질 함량은 1차 및 2차 수확할 때가 호숙기에 수확할 때보다 함량이 더 높았다. 4. 건물 수량에 대한 조단백질 총생산량은 수수의 경우 1차 및 2차 수확할 때보다 호숙기에 수확할 때 약 761 kg으로 가장 많았으며, 기장과 피는 출수기와 재생 후 수확 할 때가 호숙기 보다 상대적으로 높은 함량을 보였다. 5. 가소화양분총량은 3작물 모두 수확시기에 따른 함량 차이는 보이지 않았다.

In Sri Lanka, the shoulder in asphalt pavements has been constructed using the materials transported from borrow pit in the iRoad Project due to the low quality of in-situ soils. After excavating 150~200mm thick and 500mm wide shoulder area, the borrow pit materials are placed and compacted according to specifications. The excavated in-situ soils are dumped in designated location. It is estimated that this process of shoulder construction is not economical due to high material transportation cost and can also induce the environmental issues by disposal of in-situ soils. It can also cause distresses such as surface rutting and edge drop-off in soft shoulder section due to bearing capacity failure and off-tracking of vehicle. The heavy rainfall in Sri Lanka can induce severe erosion problem when using the soft shoulder. To improve the strength and durability of pavement shoulders in the iRoad Project, the soil stabilization will be a good alternative to solve the above mentioned problems. The use of in-situ soils with addition of soil stabilizer enables to reduce the construction cost of shoulder section and mitigate the environment issues. The objective of this task is to review the application of soil stabilization method for soft shoulder construction in the iRoad Project. Firstly, the quantitative analysis of soil strength improvement due to soil stabilization was done for soil samples collected from iRoad construction sites. Two types of soils were selected from iRoad Project sites and prepared for soil stabilization testing by the Road Development Authority. Secondly, the appropriate stabilizer was selected at given soil type based on test results. Three different stabilizers, ST-1, ST-2, and ST-3, produced in Korea were used for estimating soil strength improvements. Finally, the optimum stabilizer content was determined for improving shoulder performance. The uniaxial compressive strength (UCS) test was conducted to evaluate the strength of stabilized soil samples in accordance with ASTM D 1633. The use of bottom ash as a stabilizer produced from power plant in Sri Lanka was also reviewed in this task.

The objective of this study was to investigate the suitable method for rapid establishment of grassland according to forage species, mixed pastures and installation of mesh at 35 degree angle of cutting area in the middle region of Korea. In agronomic characteristics after wintering, vegetation coverage of Tall fescue(TF) was 74% in monoculture, which was the highest among 3 forage crops. Meanwhile average vegetation coverages of monoculture and mixed pasture were 67 and 92% in treated of mesh, which were 4 and 18% higher than untreated of mesh, respectively. In botanical composition, TF of monoculture and Orchardgrass(OG) and TF oriented mixed were indicated over 94% forage coverage. Meanwhile forage coverages Kentucky bluegrass(KBG) and Red top(RT) of monoculture and Perennial ryegrass(PRG) oriented mixture were 89, 81 and 92% in treated of mesh, which were 7, 6 and 5% higher than untreated of mesh, respectively. In forage productivity, dry matter yield(DMY) of TF(12,537kg/ha) and KBG(11,897kg/ha) of monoculture were significant(p<0.05) higher than RT(9,604kg/ha). Meanwhile DMY of OG(12,227kg/ha), TF(12,823kg/ha) and PRG(11,871kg/ha) oriented mixed were not significant difference(p>0.05). In forage quality of monoculture, in the first year, crude protein of KBG was 13.6%, which was the highest among 3 forage species. Also neutral detergent fiber(NDF) of TF was 56.5%, which was the lowest among 3 forage species. In mixed pasture, in the second year, NDF and acid detergent fiber of PRG oriented mixed were 56.5 and 34.3%, respectively, which was the lowest among 3 mixed pasture. In conclusion, forage species TF was more suitable on initial rootage and continuous forage coverage, OG and TF oriented mixed were more suitable on continuous forage coverage. Also the installation of mesh showed positive effects on initial rootage and maintain forage ratio.

This study was conducted to explore the effects of seeding rate of a seed mixture on grassland productivity and botanical composition. Seeding rates were composed of four different treatments varying by the amount of seed: T1, 50% decrease in seeding rate from standard; T2, standard seeding rate; T3, 50% increase in seeding rate from standard; and T4, 100% increase in seeding rate from standard. In 2016, seasonal changes in grass botanical composition revealed T2, T3, and T4 in portion of grass ratio were the highest with a range of 87–100%, whereas T1 was the lowest of all treatments. In 2017, the botanical composition in T1 showed that the grass ratio in of the first cutting was 91%, but in the fourth cutting its ratio decreased by 75%. The results from both years were combined for each treatment, T4 (11,435 kg ha-1) and T3 (11,162 kg ha-1) demonstrated the highest dry matter yield of the treatments (T1: 8,196 kg ha-1; T2: 9,521 kg ha-1) (p＜0.05). As a result, a 50–100% increase in the seeding rate from the standard demonstrated the best grassland yield and botanical composition.

Near infrared spectroscopy (NIRS) is a rapid and accurate method for analyzing the quality of cereals, and dried animal forage. However, one limitation of this method is its inability to measure fermentation parameters in dried and ground samples because they are volatile, and therefore, respectively lost during the drying process. In order to overcome this limitation, in this study, fresh coarse haylage was used to test the potential of NIRS to accurately determine chemical composition and fermentation parameters. Fresh coarse Italian ryegrass haylage samples were scanned at 1 nm intervals over a wavelength range of 680 to 2500 nm, and optical data were recorded as log 1/reflectance. Spectral data, together with first- and second-order derivatives, were analyzed using partial least squares (PLS) multivariate regressions; scatter correction procedures (standard normal variate and detrend) were used in order to reduce the effect of extraneous noise. Optimum calibrations were selected based on their low standard error of cross validation (SECV) values. Further, ratio of performance deviation, obtained by dividing the standard deviation of reference values by SECV values, was used to evaluate the reliability of predictive models. Our results showed that the NIRS method can predict chemical constituents accurately (correlation coefficient of cross validation, R2 cv, ranged from 0.76 to 0.97); the exception to this result was crude ash (R2 cv = 0.49 and RPD = 2.09). Comparison of mathematical treatments for raw spectra showed that second-order derivatives yielded better predictions than first-order derivatives. The best mathematical treatment for DM, ADF, and NDF, respectively was 2, 16, 16, whereas the best mathematical treatment for CP and crude ash, respectively was 2, 8, 8. The calibration models for fermentation parameters had low predictive accuracy for acetic, propionic, and butyric acids (RPD < 2.5). However, pH, and lactic and total acids were predicted with considerable accuracy (R2 cv 0.73 to 0.78; RPD values exceeded 2.5), and the best mathematical treatment for them was 1, 8, 8. Our findings show that, when fresh haylage is used, NIRS-based calibrations are reliable for the prediction of haylage characteristics, and therefore useful for the assessment of the forage quality.

This experiment was conducted to comparison study on the productivity for certified varieties of import adaptability of silage corn in Pyeongchang area. Total eight varieties (Gangpyeongok, 31N27, 32P75, 32W86, P3156, P3394, DK 689 및 DK 729) were evaluated. The experimental design was 8 treatment of randomized block with three replications. Corn varieties were cultivated in experimental field of Pyeongchang campus, SNU from 1 May to 2 September, 2015 and plot size was 15㎡. Plant height of 32W86 and ear height of Gwanpyeongok was the highest (p<0.05). Tasseling and silking date was 27 July-3 August and silking occurred after 1-3 day of tasseling. Average day to silking was 92 days and that of 31N27 variety was short (p<0.05). The varieties of DeKalbo Company (DK 689 and DK 729) required more times to silking. Average GDD (growth degree day) of eight varieties was 1,023℃ and P3352 was the lowest GDD. In the trials of resistance evaluation, P3394 was strong in disease, P3156 was the highest in insect. All varieties did not show the lodging and variety of DK 729 showed the highest stay green score (p<0.05). Average dry matter content was 30.77%, it showed higher trend in DM. 32W86 was the highest DM content among the varieties, but there was no significant difference among varieties (p>0.05). The weight per ear was the highest in 32W86 and the lowest in Gwanpyeongok. The ration of ear to whole plant was higher in 32W86 and P3394, but it was not found the significant difference (p>0.05). Average yield of fresh and DM was 59,017 and 13,476 kg/ha, respectively. DK 689 showed higher DM and TDN yield than others, but there was not significant difference (p>0.05). According to results, the difference of productivity was not found among certified variety of import adaptability of silage corn. The varieties Gwanpyeongok, 32W86 and 32P75 would be recommendable in Pyeongchang area for stable cultivation.

This study was conducted to find out the effects of over seeding using new grass varieties orchardgrass ‘Kordione’ and ‘Onnuri’, and tall fescue ‘Greenmaster’ on botanical composition and dry matter productivity from 2013 to 2016 in low productivity hilly pasture, middle area of Korea. There were used 3 grass seed mixture types made of different compositions and amounts {T1: control - no over seeding, T2: tall fescue (TF) ‘Greenmaster’ 18 kg ha-1, orchardgrass (OG) ‘Kordione’ 9 kg ha-1, Perennial ryegrass (PRG) ‘Linn’ 5 kg ha-1, and Kentucky bluegrass (KBG) ‘Kenblue’ 2 kg ha-1, T3: TF ‘Greenmaster’ 9 kg ha-1, OG ‘Kordione’ 18 kg ha-1, PRG ‘Linn’ 5 kg ha-1, and KBG ‘ Kenblue’ 2 kg ha-1, T4: TF ‘Greenmaster’ 9 kg ha-1, OG ‘Onnuri’ 18 kg ha-1, PRG ‘Linn’ 5 kg ha-1, and KBG ‘Kenblue’ 2 kg ha-1}. In the botanical composition of grassland, T1 nearly remained constant in other seasons while ratio of weeds were increased in summer season. T2 was better than control (T1) in portion of grass ratio, which has increased by 80%. In the early time of establishment, the portions of OG were increased in T2, but TF rate was increased after 3 years later when grass was established. T3 and T4 showed a very similar patterns, grass ratio had increased by 80% and the portion of KBG had increased as time passed. T2 (129,763 kg ha-1) was showed the highest dry matter yield than other treatments (T1: 6,756 kg ha-1, T3: 9641, and T4: 10,738) in 2016.

This experiment was carried out to study the effect of rice black-streaked dwarf virus(RBSDV) infection rate on forage productivity of corn varieties in Cheonan of chungcheongnamdo from 2006 to 2008. Forage corn varieties of 10 were cultivated with first cropping(seeding in the last ten days of April) and second cropping(seeding in the last ten days of May) system in field and tested the infection rates of RBSDV and productivity of forage. The Infection rate of RBSDV was significant difference between corn varieties in middle district of Korea. Resistant corn varieties for RBSDV were ‘Kwanganok’, ‘P3156’, ‘Kwangpeyongok’ and ‘P3394’ but susceptible varieties were ‘Suwon19’, ‘DK697’, ‘GW6959’ and NC+7117. Dry matter(DM) yield of forage corn according with infection rates of RBSDV in field was significant difference between varieties(p<0.05). DM yield of susceptible varieties, ‘Suwon 19’, ‘DK697’ and ‘GW6959’ was lower about 20% than that of resistant varieties, ‘Kwangpeyongok’ and ‘P3156’. For increasing the productivity of forage corn, recommend of resistant varieties for RBSDV and control of seeding time are very important in middle district of Korea.

This study was conducted to ameliorate the productive performance of Hanwoo cow using hill pasture grazing system. Twenty seven animals aged 9 were designed to divide with 3 groups which were Control (restricted concentrate and hay for 5 months), T1 (restricted concentrate and pasture grazing ad libitum for 5 months) and T2 (restricted concentrate, 30 g/head of mineral nutrients and pasture grazing ad libitum for 5 months). Average daily gains increased higher in the order of T2, Control and T1. The conception rate of Control, T1 and T2 after artificial insemination during post-grazing period were 88.9%, 100%, 100%, respectively. In conclusion, the results indicated that hill pasture grazing system might enhance not only the productive performance of Hanwoo cow but also profit for conventional feeding system.

This study evaluated the effect 3 types of conditioning (Untreated, Impeller, Roller) and the number of times for tedding (rake-teder) performed in one day on the drying rate of tall fescure hay. The experiment was performed at The forage site of Cheonan-si seongwhan-eup National Institute of Animal Sciencee. It took 3 days to make tall fescue hay. The Impeller and Roller conditioning types shortened the haymaking period by 1 day compared to untreated hay. Impeller and roller samples had less than 20% of water content by the 23rd-day at 13 o’clock. Tedding frequency failed to affect haymaking. There was no correlation between the three types of conditioning or tedding frequency and acid digestible fiber (ADF), neutral detergent fiber (NDF), total digestible nutrients (TDF), crude protein (CP), or relative feed value (RFV) in tall fescue hay. CP was 12.5~12.9% for the three types of conditioner with 1 or 2 times of tedding a day, Threrfore, conditioner types and gedding frequency had no significant effect on CP.

This study was conducted to determine the effect of rainfall after cutting on nutritive value of Italian ryegrass (Lolium multiflorum) hay. Fresh Italian ryegrass was treated with 3 days natural rainfall (56.5, 14.0 and 1.6 mm) after cutting without tedding. Rainfall effect on Italian ryegrass quality was evaluated based on Neutral detergent fiber (NDF) and acid detergent fiber (ADF), crude protein (CP), crude fat (CF), crude ash (CA) and non-fiber carbohydrate (NFC). The quality of Italian ryegrass was decreased with extending raining days. The content of neutral detergent fiber (NDF) was increased from 0.4 to 4.7% by 1 day advancement in raining. CF content was decreased from 8.1 to 32.8%. CP and CA contents did not change, but NFC content was decreased from 0.5 to 8.4%. The quality damage by the number of raining days after cutting Italian ryegrass was the lowest on the first day from exposure to rain.

The objective of this study was to determine the effect of the moisture content and pellet mill type on the physical and chemical characteristics of Italian ryegrass (IRG) pellet. Moisture content of raw material significantly (p<0.05) affected IRG pellet formation. Moisture content at 25% was the best condition for IRG pellet formation in terms of shape, power load and temperature changes. The hardness of pellet was decreased when moisture content was increase. However, the hardness of pellet was not affected by pellet mill type. Moisture content at 30% dramatically (p<0.05) decreased the durability compared to moisture content at 25%. Dry matter content of IRG pellet was increased (p<0.05) after pelleting. Total count of microorganism was decreased in pellet due to pressure heat and moisture losses during the pelleting process. These results indicated that the proper moisture content of Italian ryegrass pelleting would be at 25%. In addition, Roll & flat die type would be more suitable than Ring die and Die & flat die type in IRG pelleting. Pelleting works would be beneficial for improving forage quality and long storage.

Arsenic (As) is a toxic element that easily taken up by plants root. Several toxic forms of As disrupt plant metabolism by a series of cellular alterations. In this study, we applied annealing control primer (ACP)-based reverse transcriptase PCR (polymerase chain reaction) technique to identify differentially expressed genes (DEGs) in alfalfa roots in response to As stress. Two-week-old alfalfa seedlings were exposed to As treatment for 6 hours. DEGs were screened from As treated samples using the ACP-based technique. A total of six DEGs including heat shock protein, HSP 23, plastocyanin-like domain protein162, thioredoxin H-type 1 protein, protein MKS1, and NAD(P)H dehydrogenase B2 were identified in alfalfa roots under As stress. These genes have putative functions in abiotic stress homeostasis, antioxidant activity, and plant defense. These identified genes would be useful to increase As tolerance in alfalfa plants.

Copper (Cu) is a necessary microelement for plants. However, high concentrations of Cu are toxic to plants that change the regulation of several stress-induced proteins. In this study, an annealing control primer (ACP) based approach was used to identify differentially expressed Cu-induced genes in alfalfa leaves. Two-week-old alfalfa plants (Medicago sativa L.) were exposed to Cu for 6 h. Total RNAs were isolated from treated and control leaves followed by ACP-based PCR technique. Using GeneFishing ACPs, we obtained several genes those expression levels were induced by Cu. Finally, we identified several genes including UDP-glucuronic acid decarboxylase, transmembrane protein, small heat shock protein, C-type cytochrome biogenesis protein, mitochondrial 2-oxoglutarate, and trans-2,3-enoyl-CoA reductase in alfalfa leaves. These identified genes have putative functions in cellular processes such as cell wall structural rearrangements, transduction, stress tolerance, heme transport, carbon and nitrogen assimilation, and lipid biosynthesis. Response of Cu-induced genes and their identification in alfalfa would be useful for molecular breeding to improve alfalfa with tolerance to heavy metals.

Nitrogen (N) fertilizer management is one of the important aspects of economic production of sorghums in sustainable agriculture. The aim of the study was to evaluate the effects of different N application rates and its split N application methods on productivity, growth characteristics, N accumulation, N use efficiency (NUE), and feed value of Sorghum × Sudangrass hybrids. Treatments consisted of five N application rates (0, 150, 200, 250, and 300 kg ha-1) and two split N application methods (40% in basal N, 30% at the growing stage, and 30% after the first harvest vs. 50% in basal N and 50% after the first harvest). Plant height, leaf width, and stem diameter were increased (p≤0.05) with increasing N fertility rates at each harvest. Chlorophyll content (expressed as SPAD values) was the highest at a rate of 300 kg N ha 1 (first harvest, 46.32; second harvest, 33.09). It was the lowest at zero N (first harvest, 21.56; second harvest, 18.5). Total N, N uptake, and NUE were increased with higher N rates. Split N application had little effect on total N, amount of N uptake, or NUE. Total dry matter yields were the highest (21,715 kg ha-1) at a rate of 300 kg N ha-1. It was the lowest (10,054 kg ha-1) at zero N. Our results suggest that more than 300 kg N ha-1 can improve dry matter yield to be above 116% compared to zero N, thus enhancing the agronomic characters of sorghums. However, no significant effect had been found for split N application. Further work is needed to determine the optimal N levels and the effect of split N application rates.