The blackish cicada, Cryptotympana atrata Fabricius, 1775 (Hemiptera: Cicadidae) was originally distributed mainly in the southernmost remote island, Jeju and rarely throughout low lands in South Korea, but has been explosively increased at the urban areas, where annual temperature is higher. In this study, we sequenced a partial mitochondrial COI from a total of 171 individuals collected throughout 12 localities in South Korea. The haplotype found with the highest frequency in Jeju island shares only with two inland localities in southern region with a low frequency, whereas the haplotype found with the highest frequency throughout inland localities was not found in Jeju island. These results showed that Jeju population, southern region, and other inland populations form somewhat different genetic groups.

The large copper butterfly Lycaena dispar (Haworth, 1803; Lepidoptera: Lycaenidae) has been categorized as a near-threatened species (NT) in South Korea from 2012 mainly due to limited distribution. In this study, we visited 36 sites spread across all South Korean provinces to verify the distributional range of the species and sequenced mitochondrial COI for 53 individuals from nine sites. We observed L. dispar at 15 sites in six provinces, including the two previously known provinces, indicating a southward range expansion. The in-field monitoring and genetic data collectively suggested that L. dispar does not have a limited distribution nor is it isolated, indicating that it should be reclassified as less vulnerable. Our study demonstrates that the combination of field and genetic data can provide a more reliable assessment of the stability of a species.

The clearwing moth, Synanthedon bicingulata (Staudinger, 1887), is a pest that infests various species of cherry trees. However, genetic information regarding the genus Synanthedon including S. bicingulata, is limited. In this study, we sequenced a complete mitochondrial genome (mitogenome) of the species. The 16,255 bp of S. bicingulata mitogenome differs from the typical gene arrangement formed in Lepidoptera: trnQ-trnS2-trnM-trnI arrangement between the A+T-rich region and the ND2 junction. Moreover, the genome has untranslated repetitive sequences in the intergenic space between lrRNA and trnV, as well as the CGA start codon in COI and the TTG start codon in ATP8. Similar observations are noted in species belonging to the tribe Synanthedonini within the genus Synanthedon.

Vespa binghami (Hymenoptera: Vespidae) is one of the 10 species in the genus Vespa distributed in South Korea and is the only nocturnal wasp. In this study, we sequenced complete mitochondrial genome (mitogenome) of the species using the Sanger method and using Vespa-specific 30 primer sets. The length of V. binghami was 15,957 bp and the total A/T content was 80.6%. The A+T-rich region of V. binghami was 152 bp, and other Vespa species ranged from 39 bp (V. velutina) to 2,230 bp (V. v. auraria). Compared to the majority of insects, the gene arrangement of V. binghami had differences as followings: trnY-trnI-trnM-trnQ, trnN-trnE-trnS1-trnF, and trnS2-trnL1. However, all species in the genus Vespa registered in GenBank to date were composed of the same arrangement. Phylogenetic analysis using 13 PCGs and 2 rRNA genes showed the sister relationship between V. binghami and V. orientalis with the higher nodal supports.

Tropilaelaps mercedesae Anderson and Morgan, 2007 (Acari: Laelapidae) is a serious ectoparasite of the brood of several honey bee species. Among the four recognized species of Tropilaelaps, Korean population was renamed as T. mercedesae from T. clareae on the basis of morphological evidences and genetic data. In this study, we report the complete mitochondrial genome (mitogenome) sequence of T. mercedesae. The 15,119-bp long mitogenome has an identical gene arrangement to that of Chinese sample reported previously. Comparison of two geographic samples showed COII, ND5, ND4, ND6, CytB, and ND1 to have higher number of variable sites than COI, which is often used for population-level study, suggesting these genes to have potential usefulness for population genetic study. The mitogenome sequence of T. mercedesae from Korea could be useful for species identification for geographic samples, trace of the origin of local populations, and illustration of evolutionary distinction among Tropilaelaps species either using part of or whole genome.

Bombyx mandarina (Lepidoptera: Bombycidae), the presumed ancestor of the domesticated silkworm B. mori, has long been a subject of study to illustrate the geographic relationships in connection with origin of B. mori. We sequenced 100 mitochondrial genomes of B. mandarina collected from South Korea and Japan and these were combined with public data. Phylogenetic and population genetic analyses showed that all individuals collected in South Korea, except one individual from Inje, formed a strong group together with northern China and some individuals of southern China. This group was placed as the sister group to B. mori strains, suggesting that this group could have been served as an immediate progenitor for B. mori. For further robust inference more analysis is underway.

Spodoptera eridania and S. ornithogalli (Lepidoptera: Noctuidae), which are polyphagous pests that damage various crops such as tomatoes and beans are regulated quarantine species that are highly likely to invade South Korea. Therefore, it is crucial to promptly and accurately identify the presence of S. eridania and S. ornithogalli in crop fields to effectively eradicate as a regulated quarantine species. In this study, we developed a loop-mediated isothermal amplification (LAMP) assay, which allows for rapid in-field identification. To develop the LAMP　assay, we selected target species-specific genomic regions from the whole-genome sequences of one target and 13 other lepidopteran species. We validated each five and six primer sets that consistently produced positive reactions in S. eridania and S. ornithogalli, respectively. To test the sensitivity of the each locus, LAMP reactions were performed using various reaction times using crude DNA, which was extracted from various types of adult tissues. All sensitivity tests were also successful.

본 연구는 국내 꽃 정기구독 서비스에 대한 소비자의 인식 과 이용 현황 및 선호도를 파악하기 위해 국내 성인 남녀 중 이용자 225명과 비이용자 291명을 대상으로 설문을 진행하 였다. 대부분의 응답자들은 주로 인터넷과 SNS를 통해 꽃 정 기구독 서비스를 접하였고, 꽃 정기구독 서비스 이용자들은 주로 1~2주에 1회(76.9%), 2~4만 원 가격대(60.4%)의 혼합 된 꽃 상품을 구독하고 있었으며, 대부분 이용 기간은 1년 이 내(84.4%)였다. 꽃 정기구독 서비스를 이용하는 곳은 대부분 집(71.6%)으로, 많은 이용자들이 자기 자신(65.8%)을 위해 서비스를 이용하고 있었다. 꽃 정기구독 서비스에 대하여 이 용자와 비이용자 모두 긍정적으로 인식하고 있었으나, 이용자 들은 비이용자들에 비해 꽃 정기구독 서비스의 가격이 합리적 이고, 삶의 질이 향상되었다고 응답해, 서비스 장점에 대하여 이용자가 비이용자에 비해 더욱 긍정적인 것을 알 수 있었다 (p<0.001). 비이용자들은 꽃 정기구독 서비스 단점으로 포장 쓰레기가 많이 나올 것 같고, 신선도가 떨어질 것으로 우려하 였지만, 이와 반대로 이용자들은 그렇지 않다고 응답하였고, 그 외 단점 문항들에 대해서도 서비스 이용 시 크게 불편함을 느끼지 않는 것으로 나타났다. 응답자들은 꽃 정기구독 서비 스 이용 시 선호하는 상품으로 다양한 꽃이 2~4가지 정도 혼 합된 중간 크기(18~20cm×30~40cm, W×H) 상품을 선호 한다고 응답하였고, 꽃 정기구독 서비스 업체를 선정하기 위 해 고려해야 할 요인으로 이용자와 비이용자 모두 신선도, 꽃 구성, 구매 후기를 중요한 요인으로 응답하였다. 꽃 정기구독 서비스 이용 중 추가로 비용을 지불하면서 이용하고 싶은 서 비스에 대하여 응답자들 모두 절화보존제와 프리미엄 디자인 에 대하여 추가 비용을 지불할 의사가 있다고 응답하였다. 꽃 정기구독 서비스 이용자들은 서비스에 대하여 전반적으로 업 체가 제공하는 정보나 상품에 대해 신뢰하고 있었고, 꽃 정기 구독 서비스 비용은 합리적이라며 긍정적으로 평가하였다. 또 한, 본 서비스에 대한 재이용 의사도 높은 것으로 나타났다. 꽃 정기구독 서비스를 통해 본인을 위한 꽃 소비가 점차 늘어 나고 국내 소비자들의 화훼 소비 형태가 다양하게 변화되었음 을 확인하였으며, 이러한 결과를 바탕으로 앞으로 새로운 국 내 화훼산업 시장이 활성화되고, 지속적인 화훼 소비와 국내 화훼 문화의 발전이 이루어질 것으로 기대한다.

In this study, quantitative analysis is attempted on data collected from Chilgapsan Observatory Star Park in Cheongyang-gun, Chungcheongnam-do. The aim of this experimental study in which quantitative analysis of the Astronomical Science Museum in Korea is conducted is to investigate its current situation and secure basic data. As of July 31, 2023, it has had 283,931 cumulative visitors in total. It had the largest number of visitors when it opened (2009 year), after which their number reduced steadily until the pandemic (COVID-19, 2020–2022). Recently, however, the number of visitors has increased. Generally, the number of visitors is highest in August (20.8$\%$) and least in April (4.1$\%$). The visit rate is higher on weekends (Saturday and Sunday) than on weekdays (Monday–Friday), and groups comprise only about 5.3$\%$ of the total number of visitors. Moreover, it can be confirmed that the number of visitors increases sharply during events. Finally, it was confirmed that the visit rate was unaffected by weather conditions. Considering these results, we propose the following strategies: 1) Establish a special program that reflects “the weekend effect.” 2) Prepare a plan to attract group visitors during the weekdays using “the event effect.” 3) Arrange alternative programs (e.g., experiential activities) that can be conducted indoors regardless of weather conditions. We think that our findings will help establish a roadmap for the direction the Astronomical Science Museum should take and aid in preparing a strategic foundation to preemptively respond to unexpected situations (e.g., pandemics).

Throughfall (TF)—as a diffusive hydrological water flux—significantly affects ecohydrological and biogeochemical processes within forest ecosystems. Recent investigations have revealed the impact on TF generation processes within unmanaged coniferous plantations of under-canopy structures, particularly those laden with dead branches, as well as upper-canopy structures. However, spatiotemporal variations in TF in such plantations remain unexplored. We investigated these variations in TF in a 33-year-old unmanaged Japanese cypress (Chamaecyparis obtusa Endl.) plantation, laden with dead branches, with a high stand density (SD) of 2,500 stems ha−1. Over a two-year period (May 2017 to May 2019), we conducted weekly TF measurements using 28 manual-type TF collectors. We compared the present TF ratio and canopy water storage capacity (S) with those of previous investigations conducted on Japanese cypress plantations. Moreover, we assessed key indices contributing to spatiotemporal TF variations (canopy cover: CC and distance to the nearest stem: TFd) and potentially influential dead branch indices (number of dead branches: TFdb and vertical spacing length on a stem: TFs) to elucidate TF spatial patterns. The results showed that the TF ratio was notably lower than that in previous studies (n = 13), with SD (r = –0.92, p < 0.001) and S (r = –0.87, p < 0.001) emerging as key influential factors among other stand-structure parameters. Spatial TF patterns exhibited a decreasing trend as the gross rainfall (GR) increased. Temporal stability was not significantly associated with CC (r = 0.120, p = 0.544), TFd (r = 0.068, p = 0.731), TFdb (r = 0.211, p = 0.281), or TFs (r = 0.206, p = 0.292) for any of the TF collectors. These findings underscore the important role of GR in determining the spatial variation of TF. Collectively, our results contribute to an enhanced understanding of TF spatiotemporal heterogeneity in unmanaged Japanese cypress plantations with dead branches.

최근 기술이 발전함에 따라 다양한VR (Virtual Reality) 및AR (Augmented Reality) 장치들이 등장하고 있다. 이 러한 장치들의 대부분의 콘텐츠는 사용자 상호작용을 위해 컨트롤러에 의존하고 있으나 컨트롤러는 휴대하 기 어렵고 분실하기 쉽다는 단점이 있다. 또한, 컨트롤러를 통한 사용자 인터페이스는 장치에 익숙하지 않은 사용자들이 불편함을 느낄 수 있고 새로 인터페이스를 배워야 하는 한계가 있다. 이러한 문제를 해결하기 위 해Oculus Quest는 핸드 트래킹 기술을 도입하여 사용자가 자연스러운 손 제스처를 인터페이스로 사용할 수 있게 하였다. 그러나 핸드 트래킹을 활용하는 방법은 그 어려움 때문에 아직 널리 연구되지 않았고 아직 활 용되지 못하고 있다. 이에 VR 콘텐츠와 상호작용하는 데 손을 사용하는 방법을 탐구하고, 그것을 이용하여 콘텐츠를 만드는 방법을 설명한다

The nuclear licensee must ensure that the nuclear or radiological emergency preparedness and response organization is explicitly defined and staffed with adequate numbers of competent and assessed personnel for their roles. This paper describes the responsibilities of medical and support personnel for the medical action of casualties in the event of a radiological emergency at the KAERI. Currently, there is one medical personnel (nurse) in KAERI, and a total of eight medical support personnel are designated for medical response in the event of a radiological emergency. These medical support personnel are designated as one or two of the on-site response personnel at each nuclear facility, operating as a dedicated team of A, B (4 people each). In the event of a radiological emergency, not all medical support personnel are mobilized, but members of the dedicated medical team, which includes the medical support personnel of the nuclear facility where the accident has occurred, are summoned. Medical and support personnel will first gather in the onsite operational support center (OSC)/technical support center (TSC) to prepare and stand by for the medical response to injured when a radiological emergency is declared. They should take radiation protective measures, such as wearing radiation protective clothing and dosimeters, before entering the onsite of a radiological emergency, because injuries sustained during a radiological emergency may be associated with radioactive contamination. In the event of an injury, direct medical treatment such as checking the patient’s vitals, first aid, and decontamination will be carried out by medical personnel, while support personnel are mainly responsible for contacting the transfer hospital, reporting the patient’s condition, accompanying the ambulance, filling out the emergency medical treatment record, and supporting medical personnel. In order to respond appropriately to the occurrence of injuries, we regularly conduct emergency medical supplies education and medical training for medical support personnel to strengthen their capabilities.

The pear pest, Cacopsylla jukyungi (Hemiptera: Psyllidae), is one of the most damaging insect to commercial pears in South Korea. In this study, we developed eight microsatellite markers specific to C. jukyungi and genotyped 132 individuals collected from 11 localities throughout South Korea. Populations showed lower observed heterozygosity than expected heterozygosity and slightly or highly positive values of inbreeding coefficients, suggesting that C. jukyungi is subjected to inbreeding. The nationwide expansion of pear orchards and the replacement with a popular new cultivar during the last 50 years, which may have accompanied the spread of C. jukyungi-bearing pear grafts and scions, are likely sources of such facilitated dispersal. Thus, a management strategy against unintended anthropogenic dispersal of the pear psyllid will be required for better control of C. jukyungi.

경상북도에 위치한 산림유전자원보호구역내 2개 지역(영주지역의 신갈나무, 울진지역의 소나무)과 경기 포 천시에 위치한 생물권보전지역내 2개 지역(포천지역의 신갈나무와 소나무)에서 2016년부터 2017년까지 함정 트랩(Pitfall trap)을 매년 5회씩 5반복하여 각각 비교하였다. 그 결과 산림유전자원보호구역에서는 27과 106속 160종 2,665개체가 확인되었으며, 종풍부도는 20.28, 종균등도는 0.266, 종다양도는 1.355, 우점도는 0.265로 우점 종은 납작먼지벌레속 sp.2 (Synuchus sp.2)이였다. 또한, 생물권보존지역에서는 16과 49속 77종 4,834개체가 확 인되었으며, 종풍부도는 8.958, 종균등도는 0.301, 종다양도는 1.31, 우점도는 0.669로 우점종은 윤납작먼지벌레 (Synuchus nitidus)였다. 산림의 중요 지역 간에 지표성 딱정벌레의 군집을 비교함으로써 환경변화에 따른 산림 보존 프로그램을 수행하는데 유용한 기초 자료로 활용될 것이다.

벚나무류 수목은 생활권 수목(도시공원, 가로수 등)으로 전국에서 가장 많이 식재되고 있으며 전라 남도 또한 벚나무류 수목이 가로수 중 가장 많은 비율을 차지하고 있다. 복숭아유리나방은 농업환경 외에도 생활권 녹지공간에서도 벚나무류 수목을 가해하여 피해를 발생시키고 있는 것으로 보고되고 있으나, 생활권 수목과 산림에서의 관련 연구는 그 필요성에 비해 미흡한 상황이다. 본 연구는 전라남 도 내 생활권 수목을 기주로 하여 발생하는 복숭아유리나방의 발생소장을 조사하여 생활권 녹지공간 을 관리하는 기초자치단체들의 효용성 있는 방제 전략 수립에 기여하고자 한다. 복숭아유리나방의 발생 확인을 위해 육안 확인이 용이한 피해흔을 세 가지로 분류하였으며, 성페로몬 루어와 트랩으로 복숭아유리나방 성체를 유인･채집하였다. 그 결과, 4월 중순 첫 우화를 확인하였으며, 5월 중순과 8월 중순에 2번의 우화 최성기를 확인하여 이때의 유효적산온도를 조사하였다. 또한, 채집된 개체들 의 종 확인 및 유전적 다양성 확인을 위해 mitochondrial 내 cytochrome oxidase subunit I (COI) 유전자 염기 서열의 분석을 수행하였다.

국내 유입 가능성이 높은 검역 관리해충인 Spodoptera eridania 및 S. ornithogalli는 전 세계적으로 토마토, 콩 등 여러 종의 작물을 가해하는 광식성 해충이다. 이에 따라 국내 유입 시 해당 작물에 높은 경제적 피해를 입힐 가능성 이 있으므로 신속 정확한 진단이 필요한 실정이다. 따라서 본 연구에서는 상기 두 종을 대상으로 현장 활용이 가능한 LAMP 진단법 개발을 수행하였다. 표적종 두 종 및 비표적종 11종(국내 발생 Spodoptera 종 및 동일 기주 가해종 등)의 전장유전체 정보를 확보한 후 비교 분석을 통해 각 표적종 별 특이적 영역을 확보한 후 해당 영역을 대상으로 LAMP 프라이머를 제작하였다. DNA 농도 10 ng/μL, 반응시간 40분을 기준으로 LAMP 진단을 수행한 결과, Spodoptera eridania는 5개의 LAMP 진단 마커를 개발하였고, S. ornithogalli는 3개의 LAMP 진단 마커를 개발하였다.

The fall armyworm (FAW), Spodoptera frugiperda (Lepidoptera: Noctuidae), which is native to tropical and subtropical regions of the Western Hemisphere is now annually arrives in Korea. In this study, we developed loop-mediated isothermal amplification (LAMP) assay, one of the main merits of which is a rapid identification of target species. Five among 11 FAW-specific loci tested successfully provided a consistent reaction when ten FAWs, which were collected from eight localities in four countries were tested, whereas the 13 non-target species were not amplified. To increase in-field applicability of the method all life stages, reaction time, and different periods after death was tested using the quick extracted DNA. Our FAW diagnostic protocol can be completed within 30 min, from the process of extracting genomic DNA from an egg or a 1st instar larva to species determination.

Ceriagrion nipponicum (Odonata: Coenagrionidae) is listed as climate-sensitive indicator species in Korea and now expands its range northward. In this study, we sequenced complete mitochondrial genome (mitogenome) of the species collected from South Korea for comparatively analysis in damselflies and to detect the genes suitable for subsequent population genetic study. Comparison of the mitogenomes from two geographic samples of C. nipponicum showed the highest variation in ND4 and ND1, whereas no variation was detected in COI, warranting usefulness of the two genes for subsequent population-level study. Phylogenetic analysis using 13 PCGs and 2 rRNAs in Zygoptera showed non-monophyletic Coenagrionidae, forming two groups.

Bombyx mandarina (Lepidoptera: Bombycidae), the presumed ancestor of B. mori, has long been a subject of study to illustrate the geographic relationships in connection with origin of B. mori. We report 97 mitochondrial genome (mitogenome) sequences of B. mandarina collected from Korea and Japan. Phylogenetic and population genetic analyses showed that all individuals of B. mandarina collected in Korean localities formed a strong group together with all individuals originated from northern China (mainly north of the Qinling-Huaihe line) and some of southern China. This group was placed as the sister group to B. mori strians suggesting that this group had been served as an immediate progenitor for B. mori.