ICT 기반의 해충 이미지 전송 시스템은 담배거세미나방과 파밤나방을 예찰하고 방제하는데 필요한 효과적인 유인 해충 전송수단이다. 시설 온실내 담배거세미나방과 파밤나방을 유인하기 위하여 스마트 트랩을 이용하였다. 스마트트랩은 나방류 해충유인을 위해 성페로몬 4종 장착하였으며 유인된 해충은 특수 카메라를 이용하여 이미지 촬영이 가능하였다. 또한, 해충유인 발광트랩 처리구는 황색광을 사용한 발광 트랩을 사용하였다. 또한 대조구로 기존에 널리 사용되는 델타트랩은 한 종류의 성페로몬을 장착하여 유인력 검정에 이용하였다. 성페로몬 한 종류만 사용한 델타트랩 처리는 담배거세미나방 페로몬 루어를 사용하였고, 4종류의 성페로몬을 설치한 스마트 트랩 처리구는 담배거세미나방, 뒷날개흰나방, 파밤나방, 왕담배나방용 성페로몬 루어를 사용하였다. 실험은 2018년 6월 1일부터 8월 31일까지 함안 시설원예연구소의 13m2의 소형비닐하우스에서 수행하였으며 공시작물은 파프리카 ‘시로코’ 품종과 토마토 ‘데프니스’ 품종을 사용하였다. 나방류 해충의 유인력 검정결과 파프리카와 토마토를 식재한 온실 모두 성페로몬 루어 한 종류를 설치한 델타트랩보다 유인발광 트랩 처리구에서 높은 나방류 유인율을 나타내었다. 시험기간 유인발광 트랩의 경우 유인 포획된 총 나방수는 파프리카 온실에서 평균 97마리, 토마토 온실에서 75마리가 유인 포획되어 가장 높았다. 그러나, 성페로몬 1종 및 4종을 설치한 델타트랩 및 스마트트랩처리구는 두 개의 온실 모두 평균 5마리 이하의 유인율을 나타내었다. 따라서, 시설내 나방류 예찰 및 유인 방제하기 위해서는 기존 성페로몬만 사용하기 보다는 유인발광 트랩을 함께 설치하는 것이 나방류 유인포획에 효과적이라고 판단되었다.

The tobacco cutworm, Spodoptera litura(Fabricius), is a major pest of tomato and frequently demands control measures. The timing of insecticide application is a key factor in determining its efficiency, so an experiment was designed to investigate this. Application of insecticide was based on three criteria: (i) the number of trap-caught moths in a Delta-type trap with a commercial sex pheromone lure placed in the center of the target area, soon after plant emergence; (ii) the percentage of plants exhibiting pinhole-type damage (10% or 20%) and (iii) the percentage of plants exhibiting shot hole-type damage (10% or 20%) compared to a check plot without any control measures. We found that the number of trap-caught moths was, compared to the other methods, the best means of deciding on insecticide application in tomato plant to control the tobacco cutworm. Using pheromone traps, we obtained the best performance of the insecticide Shinnago, causing > 90% larval mortality. Without insecticide application, tomato yield reduction due to the tobacco cutworm larva damage was 27%.

Human body and head lice are obligatory human ectoparasites. Although both body and head lice belong to a single species, Pediculus humanus, only body lice are known to be a vector of several bacterial diseases. The higher vector competence of body lice is assumed to be due to their weaker immune response than that of head lice. To test this hypothesis, immune reactions were compared between body and head lice following infections by two model bacteria, Staphylococcus aureus and Escherichia coli, and a human pathogen, Bartonella quintana. Following dermal or oral challenge, the number of these bacteria increased both in hemocoel and alimentary tract of body lice but not in head lice and the viability of the B. quintana was significantly higher in body louse feces, the major route of infection to human. In addition, body lice showed the lower basal/induced transcription level of major immune genes, cytotoxic reactive oxygen species and phagocytosis activity compared with head lice. These findings suggest that a reduced immune response may be responsible, in part, for the increased proliferation and excretion of viable bacteria which are associated with the high level of human infectivity seen in body versus head lice.

The present investigation establishes deltamethrin resistance in the common bed bug, Cimex lectularius, populationcollected from New York City (NY-BB). The mortality resistance ratio indicated that NY-BB population was 264-fold more resistant to 1% deltamethrin in contact bioassay compared to one insecticide- susceptible population collected in Florida (FL-BB). Specific enzyme activities (general esterase, glutathione S-transferase, and 7-ethoxycoumarin O-deethylase) of NY-BB were not statistically different from those of FL-BB, indicating that the metabolic factors are not associated with the deltamethrin resistance in NY-BB. Complementary DNA fragments that encoded the open reading frame of voltage-sensitive sodium channel α-subunit genes from the FL-BB and NY-BB were obtained by homology probing PCR and sequenced. Sequence alignment of the internal and 5’ and 3’ RACE fragments generated a 6500 bp cDNA sequence contig, which was composed of a 6084 bp open reading frame (ORF) encoding 2027 amino acid residues and 186 bp 5’ and 230 bp 3’ untranslated regions (5’ and 3’UTRs, respectively). Sequence comparisons of the complete open reading frames of the sodium channel genes identified two point mutations (V419L and L925I) that were presented only in the NY-BB population. L925I, located the intracellular loop between IIS4 and IIS5, has been previously found in the pyrethroid-resistant populations of whitefly (Bemisia tabaci) that was more than 100-fold resistant to fenpropathrin. V419L, located in the IS6 transmembrane segment, is a novel mutation. This evidence suggests that the two mutations are likely the major resistance-causing mutations in the deltamethrin-resistant NY-BB via a knockdown-type nerve insensitivity mechanism.

Background : Five medicinal herbs have been selected from the preliminary screening for in vitro anti-allergy activity (in RBL-2H3 cells). The present study is conducted to investigate the inhibitory effects of the medicinal herbs on allergic inflammation in other kind of cells. Methods and Results : Cells treated with five extracts prepared from Betula costata Trautv. (aerial part), Camellia sinensis L. (aerial part), Polygonatum stenophyllum Maxim. (root), Pyrus pyrifolia var. culta (Makino) Nakai (leaf), and Rehmannia glutinosa (Gaertn.) Libosch. ex Steud. (root) were measured for mRNA levels of TNF-α on HaCaT keratinocytes stimulated by TNF-α /INF-γ and for mRNA levels of IL-2 in Jurkat T cells mediated by PMA/A23187. Pre-treatment with the five extracts reduced the mRNA levels of TNF-α in HaCaT cells and mRNA levels of IL-2 in Jurkat T cells. In particular, the leaf extract of Pyrus pyrifolia var. culta (Makino) Nakai significantly and dose-dependently decreased the mRNA levels of TNF-α and IL-2. To determine the toxicity of the extracts from the selected medicinal herbs to HaCaT cells and Jurkat T cells, the viabilities of the cells treated with several concentrations of the five extracts were measured by MTT assay. Extracts of Polygonatum stenophyllum Maxim. (root), Pyrus pyrifolia var. culta (Makino) Nakai (leaf), and Rehmannia glutinosa (Gaertn.) Libosch. ex Steud. (root) (up to 250 ㎍/㎖) did not show cytotoxic effects on HaCaT cells and Jurkat T cells. On the other hand, 250 ㎍/㎖ of extracts of Betula costata Trautv. (aerial part) and Camellia sinensis L. (aerial part) reduced cell viability in both cells. Conclusion : These results demonstrate that the leaf extract of Pyrus pyrifolia var. culta (Makino) Nakai has an anti-inflammatory effect by inhibiting pro-inflammatory cytokine expression. Therefore, the leaf of Pyrus pyrifolia var. culta (Makino) Nakai can be a useful resource for the development of anti-allergy/anti-inflammatory materials.

Happymi is a new sweetpotato variety developed from Mokpo Experiment Station, National Institute of Crop Science(NICS), RDA in 2004, which was for the raw eating and processing. This variety was selected from the cross between Chinmi andMA 3 in 1996, seed

Biomi is a new sweetpotato variety developed from Mokpo Eeperiment Station, National Honam Agricultura iment Station (NHAES), RDA in 2003, which was for the utilization as feed and food for storage root ofhigh moisture content andfast fleshed up. This var

In order to get abundant germplasm for develop- ing new corn varieties, major morphological characteristics of ear were evaluated with a total of 210 Korean indigenous maize lines collected from various parts of Pusan City and Kyungnam Province, Korea. The ear shape of Korean indigenous maize was mainly cone type or similar to cone type. Cylinder type or similar to cylinder type of corn was collected from the north-west mountainous. region of Kyungnam Province, whereas cone type or similar to cone type was collected from the plain region. In the kernel colors of each ear, ears with mono colored kernels were 55.7% of the total, and ears with two to four mixed colors were 44.3%. Among the mono colored ears, brown was the most abundant at 16.8%. There was an average of 12∼14 rows per ear. Kernal rows per ear varied from 8∼20. Waxy corn was predominant at 67.2%, mixed corn with waxy kernel and common kernel at 11.0%, and common corn at 21.8%.

한국 재래종 옥수수의 염색체 특성을 알아보기 위하여 재래종 옥수수 10개의 자식계통 C-banding법으로 염색하고 염색체상에 존재하는 heterochromatic knob 수를 조사한 결과는 다음과 같았다. 1. Knob수는 6∼12개이었고 평균 9.0개이었으며 계통별로 차이가 있었다. 2. 염색체의 장완과 단완의 비율, 상대적 길이 등을 비교해 보기 위하여 Waesungri와 PI213-749 두 계통을 조사해 본 결과 계통별로 차이가 있었다. 즉, 장완과 단완의 비율은 2번 염색체의 경우에만 1.25로서 동일하였고 다른 염색체의 경우는 모두가 다르게 나타났다. 염색체의 상대적 길이는 일반적으로 Waesungri에서 크게 나타났는데 1번 염색체의 경우 Waesungri에서는 223이었고 PI213749에서는 192이었다. 3. 염색체의 상대적 길이, 장완과 단완의 비율, 그리고 knob의 위치 등을 나타내는 모식도를 통하여 두 계통의 염색체 특성을 보다 명확하게 비교할 수 있었다.

Giemsa C-banding 방법에 의하여 한국 재래종 옥수수 핵형 및 염색체의 특성을 알아보고자 옥수수의 근단 생장점을 이용하여 조사 분석하였다. 이 방법은 한국 재래종 옥수수의 핵형 분석 및 염색체의 특성 연구에도 효과적으로 활용할 수 있는 방법임이 확인되었다. 조사된 재래종 옥수수에서는 각각 10개의 heterochrornatic knob이 발견되었으며 이것들의 크기와 위치는 계통별로 다른 것으로 나타났고, 모두가 6번 염색체에 부수체를 가지고 있었다. 10번 염색체를 100으로 해서 비교해 본 각 염색체들의 상대적 길이는 조사된 계통별로 큰 차이가 있었다.