‘Hyesun’, a new cultivar of leaf lettuce (Lactuca sativa L.) with sharply wrinkled oval and red leaf, produced by a cross between 'Nongjeockchima’ (red leaf color) and ‘Jeockchima’ (high yield) has late bolting and high yield in the field. The cross and selection for advanced lines had been done by the pedigree method from 2002 to 2008. The ‘Hyesun’ has gray seed color and medium elliptic leaves. The yield and adaptability of the advanced lines were evaluated at several locations of Korea (Gangwon-do, Gyeonggi-do, Chungcheongbuk-do, Jeollabuk-do, Gyeongnam-do, and Jeju-do) for 3 years (from 2009 to 2012). The type of maturity of ‘Hyesun’ is early-medium. The leaf hardness of ‘Hyesun’ is a 1,237kg/cm2, which is not thicker than ‘Yeolpungeokchima’. The anthocyanin content of 'Hyesun' is a 88.32mg/100g, which is lower than that of ‘Yeolpungeockchima’. The BSL (Bitter Sesquiterpene Lactones, latucin + 8-deoxylactucin + lactucopicrin) content of ‘Hyesun’ is a 5.0ug/g of DW, which is lower than that of ‘Yeolpungeockchima’. Compared to ‘Yeolpungeockchima’, marketable yield of ‘Hyesun’ was higher by 7% (at 381g per plant) and the bolting time of ‘Hyesun’ was remarkably delayed under high temperature cultivation in the field. The shelf-life of ‘Hyesun’ was four weeks longer than ‘Yeolpungeockchima’ at 4℃. Furthermore, because ‘Hyesun’ is more crispy and sweeter than ‘Yeolpungeockchima’, ‘Hyesun’ has the better taste. So, we recommend that the new cultivar ‘Hyesun’ can be suitable for cultivation in both spring and fall season.

본 연구는 최근 항산화활성이 우수하다고 알려지면서 재배면적이 증가하고 있는 아로니아의 소비촉진을 위해 아로니아에 사과를 부재료로 첨가하여 떫은맛은 감소되고 기호도가 향상이 된 아로니아 혼합잼을 다양하게 제조한 후, 그 품질특성을 조사하고자 하였다. 100% 아로니아 잼을 비롯한 90%, 70%, 그리고 50% 아로니아 잼을 제조한 뒤 4가지 잼의 품질을 조사한 결과, 아로니아의 함량이 증가할 수록 pH는 감소하였고, 총산은 증가하였다. 색도는 아로니 아 함량이 감소할수록 명도는 증가하였고, 적색도와 황색 도는 아로니아 함량이 감소하여도 큰 변화를 보이지 않았 다. 조직감에서 견고성과 탄력성, 점성, 씹힘성은 아로니아 함량이 100%일 때 가장 높았다. 총 폴리페놀과 전자공여능 은 아로니아의 함량이 줄어들수록 감소하는 경향을 보였 다. 관능평가에서 색상과 향미, 신맛은 아로니아 함량이 증가하여도 유의적인 차이를 보이지 않았다. 빵 발림성과 단맛은 아로니아 함량이 감소할수록 증가하였다. 전반적인 기호도는 아로니아 함량이 70%일 때 가장 선호도가 높았 다. 따라서 생리활성, 관능적인 면을 고려했을 때 70% 아로 니아와 30% 사과를 첨가한 아로니아 혼합잼의 경우 현재 한정된 범위의 가공식품의 재료로 쓰이고 있는 아로니아의 이용성을 증진시킬 수 있을 것이라 기대된다.

Lunasin is a unique 43-amino acid peptide which has shown a chemopreventive in mammalian cells and in a skin cancer mouse model. In search for new sources of lunasin and the role of cereals in cancer prevention, we report here the properties of lunasin purified from millet. Stability of millet lunasin was measured by in vitro digestibility assay using pepsin and pancreatin. Inhibition of HAT (histone acetyltransferase) and nuclear localization in mammalian cells were used to measure lunasin bioactivity as the cancer chemopreventive agent. Lunasin present in millet crude protein was stable to pepsin and pancreatin in in vitro digestion and inhibited the activities of HATs. When added exogenously, lunasin purified from millet internalized in the nuclei of mouse fibroblast cells. On the base of this result, we conclude that lunasin in millet is bioactive and consumption of millet may play an important role on cancer prevention in millet-consuming populations.

A new early maturing satsuma mandarin (Citrus unshiu Marc.) ‘'Haryejosaeng’' was developed as a nucellar seedling selection of ‘'Tachima Wase’' (C. unshiu Marc.) that was crossed with C. natsudaidai Hayata at the National Institute of Subtropical Agriculture in Jeju island in 1992. The ‘'Jegam ga No. 2’', a first selection from the seedlings fruited in 2001 was finally named “"Haryejosaeng”" through field evaluation trials at three locations in Jeju island from 2003 to 2004. ‘'Haryejosaeng’' produces seedless fruit maturing in early November, and has higher soluble solids and lower acidity than ‘'Miyagawa Wase’', the leading early-maturing satsuma mandarin cultivar in Jeju island. Fruit weight is moderate at about 80～90 g and its shape is compressed-oblate globose with a light orange color. The rind thickness of about 2 mm provides easy peeling. The flesh shows light orange colored and contains 10 to 11 Brix and 1 to 1.1% acidity when mature. Tree shows vigorous growth and spreading thornless twigs. Alternate bearing is similar to ‘iyagawa Wase’ ‘aryejosaeng’is susceptible to citrus scab disease and melanose, but resistant to citrus canker.

Lunasin is small subunit peptide of coded from Gm2S-1 gene in soybean. It has been previously demonstrated that lunasin is a novel and promising cancer preventive peptide. Lunasin peptide is found only in the seed and not other tissues. And lunasin peptide starts to appear at 5 weeks after flowering and remains in the mature seed. We report here firstly lunasin peptide identified from soybean callus induced by the tissue culture and demonstrate its anticancer properties. The lunasin was identified and purified from soybean callus aged for 6 months. The callus lunasin(1μm) inhibited the acetylation of histone H3 and H4 by 58.8% and 56.5%, respectively. And it fully inhibited foci formation compared to the values of the positive control(no lunasin) and negative control(no MCA). Purified lunasin was able to internalize into the cell and localized in the nucleus.