Potato is the fourth major food crop mainly grown in temperate climate zone and this region is expected to be mostly affected by upcoming global warming followed by arctic zone. Such climatic change can cause the decrease of potato crop yield. Thus, climate change impact assessment on potato growth and yield has become a great importance for construing proper adaptation strategies. Crop growth model would be a powerful tool for this purpose even if the model is calibrated and validated under the wide range of environmental condition. The objective of the present study was to calibrate and validate the SUBSTOR model using the experimental data collected in the field and temperatured-controlled plastic houses. The planting season experiments for collecting data was conducted in field and temperat-controlled plastic houses by employing three potato cultivars; Daeji, Sumi, and Chubaek at the Seoul National University Farm in Suwon during 2008 and 2009. Four plastic houses used for this experiment were controlled to ambient temperature (aT, aT+1.5℃, aT+3.0℃, aT+5.0℃), respectively. The model was calibrated by adjusting the genetic coefficients to the experimental data collected, and validated with the experimental data independent of calibration. The model simulated tuber yield very close to the observed, showing R²=0.92 for Daeji and R²=0.97 for Sumi. However, the model showed a little poor performance of R²=0.61 for Chubaek, Also, SUBSTOR-potato model showed low stability at water stress environment in this experiment.