Background: Leydig cells, crucial for testosterone production, express ion channels like ANO1 that influence hormone secretion. This study investigates the expression and role of the Tandem of P domains in a weak inward rectifying K+ channel-related Acid-Sensitive K+-1 (TASK-1) channel in these cells, exploring its impact on testicular function and steroidogenesis. Methods: TASK-1 expression in Leydig cells was confirmed using immunostaining, while RT-PCR and Western Blot (WB) validated its expression in the TM3 Leydig cell line. The effect of a TASK-1 channel blocker on cell viability was assessed through live/dead staining and MTT assays. Additionally, the blocker’s effect on testosterone secretion was evaluated by measuring testosterone levels. Results: Immunohistochemical analysis revealed a predominant presence of TASK- 1, along with c-Kit and ANO-1, in Leydig cells adjacent to seminiferous tubules and also in Sertoli and spermatogenic cells. Expression levels of TASK-1 mRNA and protein were significantly higher in TM3 Leydig cells compared to TM4 Sertoli cells. In addition, blocking TASK-1 in TM3 cells with ML365 induced cell death but did not affect LHinduced testosterone secretion. Conclusions: These findings suggest that TASK-1 in Leydig cells is crucial for their viability and proliferation, highlighting its potential importance in testicular physiology.

When exposed to different types of bacteria in the oral cavity, denture based resins are prone to bacteria attachment. The purpose of this study was to investigate the physical, biological, and antimicrobial properties of denture base resins coated with Peony extract (200, 400, and 600 μg/mL). Specifically, the surface properties (microhardness, contact angle, and color change of the coated specimens), cell cytotoxicity (measured using MTT assay), and antimicrobial activity (against S. mutans (Streptococcus mutans) and C. albicans (Candida albicans) using a growth inhibition assay) were evaluated. The polyphenol content was measured using ultraviolet-visible (UV-vis) spectrometry. The experimental groups (specimens coated with Peony extract) and a control group (specimens coated without Peony extract) were statistically compared using a one-way analysis of variance and Tukey’s post-hoc tests. No statistically significant differences in surface properties or cell cytotoxicity were observed, which demonstrated their biocompatibility. Conversely, a statistically significant difference in antimicrobial activity was observed between the experimental and control groups after 48 h. This confirms the antimicrobial activity of the denture base resin coated with Peony extract and demonstrates that it is a promising dental material for preventing stomatitis.

Yam is an important tuber crop nutritionally, socio-culturally, and an income generator for many Nigerian. This explains its high demand both locally and internationally, causing increased production as Nigeria leads world production with about 45.41 million metric tons annually. The peculiarity of yam production in Nigeria entails lower yield, high cost of production, and post-harvest losses. Yam produced in Nigeria falls far below the potential crop yield, therefore, it is essential to close these yield gaps through the provision of solutions to the different constraints that hamper sustainable yam production. Despite the challenges, there is still insufficient information on the impact of policies, weeds, pests, and diseases which constitute major constraints. Nigerian farmers mostly utilize indigenous cultural approaches to manage yam production. Their goal of production is to produce yam cultivars that are suitable mainly for food products leading to the neglect of other cultivars. Few selected institutions with a yam mandate have been working on improving the production of D. rotundata and D. alata through breeding yam cultivars suitable for consumption and resilient against pests and diseases. However, more extensive studies should focus on breeding programs for yam resilient under drought and flooding, with peculiar physicochemical properties that give unique textual qualities considered in pounded yam and other yam food products. In this review paper, the significance and limitations of yam production in Nigeria and her efforts to achieve sustainable production are extensively studied.

Maize is an important cereal crop widely grown in all of Nigeria’s agroecology based on its economic and food values. This has led to its optimum production with Nigeria ranking top 12th maize producer in the world. Presently, the fall armyworm (FAW), Spodoptera frugiperda is considered a potential threat to food security in Nigeria due to its devastating feeding pattern on maize and its wide host range. Its invasive and high dispersal nature enhances its spread from its first reported state (Oyo) in early 2016 to the entire 36 states of Nigeria by the year 2018. It accounts for averagely an estimated 34% or more yield reduction in Nigeria’s maize field, hence, a need for an efficient management approach. Although most farmers rely on the use of insecticide, it is found ineffective because of the inaccessible location of FAW in maize. This study was carried out to investigate the different management approaches adopted in Nigeria to control FAW and its impact on the insect pest population. Conventional measures such as planting improved seeds, avoidance of late planting, and polyculture to increase the natural enemy’s population have been used but with limited control of FAW. Maize lines with aflatoxin resistance or transgenic Bt-maize are found resistant to FAW. Botanicals such as neem extract lowered the percentage of leaf area damage, incidence of FAW larva infestation, and the number of larvae. It is difficult to curtail the menace of FAW through one control method, hence, the need for an integrated approach

Soybean is an important crop widely grown in the northern region of Nigeria for human and animal consumption. Commercial production of soybean in Nigeria has existed for eight decades. Current malnutrition and nutrient health problems in Nigeria warranted the introduction of high nutritive and affordable food sources such as soybean in the Nigerian diet. The high demand for soybean and its products has led to the rise in the cultivation of soybean in Nigeria. Although an increase in production has been recorded, the region of production has failed to achieve the potential yield per hectare. In improving soybean production in Nigeria, it is paramount to understand the factors that contribute to the process of production. This review paper provides information on factors affecting soybean production across the regions of production in Nigeria. The identified factors responsible for the yield gap include the impact of climate change on the agroecology shift, nutrient depletion and soil fertility, sowing date, the emergence of pests and diseases, and limited improved soybean cultivars. Adoption of improved soybean variety suitable in agroecology and sowing, at the appropriate sowing date by Nigerian farmers, can guarantee optimum soybean production. Further soybean breeding improvement studies are needed to provide more improved varieties with superior performance in Nigeria’s agroecology to achieve yield potential.

The role of transient receptor potential vanilloid receptor-1 (TRPV1) has been primarily investigated in pain sensory neurons. Relatively, little research has been performed in testicular cells. TRPV1 is abundantly expressed in Leydig cells of young adult mice. This study was conducted to determine the role of the TRPV1 channel in Leydig cells. TRPV1 modulators and testosterone were treated to the mouse Leydig cell line TM3 cells for 24 h. Capsaicin, a TRPV1 activator, dose-dependently induced cell death, whereas capsazepine, a TRPV1 inhibitor, inhibited capsaicin-induced cell death. Testosterone treatment reduced capsaicin-induced cell death. High concentrations of testosterone decreased TRPV1 mRNA and protein expression levels. However, TRPV1 modulators did not affect testosterone production. These results showed that capsaicin induced cell death of Leydig cells and that testosterone reduced capsaicininduced cell death. Our findings suggest that testosterone may regulate the survival of Leydig cells in young adult mice by decreasing the expression level of TRPV1.