Transgenic animals production tools have been valuable for research and purpose. The current methods of gene transfer, microinjection and nuclear transfer, which are widely used in transgenic animal production, but all most methods has only had limited success in production of larger species. Here, we report the possibility of a sperm-mediated gene transfer method in porcine embryos. Oocytes were collected from ovaries harvested at a local slaughterhouse were matured in 500 drops of TCM-199 under mineral oil at 38.5 in a humidified atmosphere of 5%CO2 in air. After 42-43h of in vitro maturation oocytes were denuded. for sperm injection into the cytoplasm of the porcine oocytes, sperm suspension in NIM medium are subjected extraction with TritonX-100 before mixing with a green fluorescent gene (GFP). Sperm with Tritonx-100 were prepared by adding TritonX-100 to a final volume of 0.05% in the sperm suspension and mixing by trituration for 60s before two wishes in NIM medium at 2. A(ter wishing, sperm were mixed with TritonX-100 at followed by washes at 2. Sperm were resuspended in ice cold NIM to a final volume of 400 and 2-20ng/ DNA were triturated on ice for 60s. All microinjection was performed in HEPES-buffered CZB medium at room temperature within 2h. After culture in NCSU-23 for 72h, percent of porcine embryos transfected GFP gene are 20.7%(6/29) in 20ng/ sperm-DNA mixed group and other groups were 3.7 %(2/54)and 4.7%(3/67). These data suggests that sperm-mediated gene transfer method should be used to the production tool of transgenic pig efficiently.

본 연구에서는 연질 폴리우레탄 발포제 제조에서 여러 발포제와 내부온도, 기계적 물성과의 관계에 대해서 연구, 조사하였다. 발포제로는 물을 주발포제로 하여 보조발포제는 CFC-11(trichlorofluoromethane), HCFC-114b(dichlorofluoroethane), dichloromethane, n-pentane, iso-pentane, cyclopentane을 사용하였다. 기계적 물성을 측정하기 위하여 연질 폴리우레탄 발포제의 밀도가 0.015±0.002g/cm3와 0.024±0.002g/cm3인 두 종류의 발포제를 제조하였다. 연질 폴리우레탄의 발포제의 제조시 발포제별로 내부온도를 측정하였으며, 48시간 경과 후 밀도, 인장강도, 신장율, 인열강도, 압축강도, 압축영구변형률을 측정하였다. 연구 결과 dichloromethane과 cyclopentane이 보조발포제로 가장 적합하였다.

α-sulfonated fatty acid polyethylene glycol esters with polyethylene oxide(addition, 3, 5, 10mol) were synthesized through esterification of α-sulfonated fatty acid methyl esters with alkyl chain length C12~C18. Their compounds were separated with column chromatography, and confirmed by TLC. Quantitative analysis of all the sulfonates were performed according to JIS K-3362 method, and ethylene oxide unit number were determined by ISO 2270 method. Structural properties of α-sulfonated fatty acid methyl esters and their derivatives were also identified from IR, and 1H NMR spectra.

All the surface activities including surface tension, foaming power, foam stability, emulsifying power, dispersion effect, and detergency were measured and critical micelle concentration(cmc) was evaluated in dilute aqueous solution. The cmc evaluated by the Ring method was 10-3~10-4mol/L in case of monoesters, and 10-3~5.0×10-5mol/L in case of diesters, respectively. Surface tension of the aqueous solution was decreased to 45~50dyne/cm, showing the tendency that the ability of lowering the surface tension was dependent on increasing of carbon atom number in alkyl chain. Foaming power of all the monoesters was better than that of diesters. while foam stability of diesters was to the contrary. Emulsifying power of soybean oil or benzene was specially expected to be good for emulsifiers in industrial application fields. HLB values of monoesters and diesters evaluated by Griffin's method were in the range of 8 to 12. Dispersion property of ferric oxide was stable in the range of 4.5×10-5~5.0×-4mol/L in case of monoesters, and 10-5~10-4mol/L in case of diesters.

케탈이나 아세탈반응에 의하여 이루어진 1, 3-디옥솔란 고리가 산수용액 중에서 불안정해 쉽게 가수분해되는 성질을 이용하여 소수부로는 지방산의 탄화수소사슬을 친수부로는 소디움 카르복시산(Soap)을 도입하여 분해 후 계면활성을 띠지 않는 분해성 계면활성제이다. 이 화합물의 수용액에 대한 표면장력은 31dyne/cm였고, 임계미셀농도는 1.0×10-3mol/L이었다. 기포력은 비교적 좋지 않았으며, 유화력은 콩기름보다 벤젠에서 양호하였다. 또한 이 화합물의 산분해 특성을 1wt% 수용액과 벤젠 사이의 계면장력 변화에 의하여 측정한 결과 pH 1 ~ 4범위에서 약 300분 안에 모두 가수분해됨을 확인하였다.

미셀반응, 유화중합, 상간이동촉매반응 등에 이용되는 계면활성제가 갖고 있는 문제점을 해결하기 위하여 반응 후 분해되는 분해성 계면활성제를 합성하였다. 케탈이나 아세탈반응에 의하여 이루워진 1,3-디옥솔란 고리가 산수용액 중에서 불안정해 쉽게 가수분해로 계면활성을 띠지 않는 분해성 계면활성제를 합성하였다. 합성된 화합물은 백색결정상태로 얻었으며 수율은 90% 이상이었다. 그리고 이 화합물과 중간생성물은 얇은막 크로마토그래피와 컬럼 크로마토그래피로 분리하여 적외선과 수소핵자기공명 및 원소분석 스텍트라로 그 화합물들의 구조를 확인하였다.