Proper oral hygiene is required to maintain oral health and prevent oral disease. Toothbrushing is central to proper oral hygiene. Mechanical tooth cleaning is the mainstay of plaque control. A variety of toothbrushing techniques have been developed and evaluated for their efficacy. However, these evaluations are subjective. To adequately evaluate toothbrushing objectively, a novel toothbrushing monitoring system was developed. The system involves user-monitored brushing patterns including toothbrush motions using a 3-axis gyroscope, 3-axis accelerometer, 3-axis magnetic sensor, one load-cell and Bluetooth devices. To confirm the efficacy of this toothbrushing monitoring system, eight periodontist performed tooth brushing in a dental model, and their brushing motion was monitored and recorded, and evaluated by statistical means. The proposed monitoring system can be used to aid dental care personnel in toothbrushing instruction.

Recently an outbreaked pest belongs to Hemiptera: Recaniidae, Ricanula sp. is greatly concerned about the outspreading throughout the South of Korea by wide range of host, including Cornelian cherry, Jujube, and peach trees. In Chungbuk province, this pest was first occurred at Jincheon and Okcheon in 2012, Cheongju in 2013 and now found out at Eumseong and Goesan in 2015. Ricanula sp. was oviposited directly into one-year twig, did damages on fruit-bearing formation and finally withered the host. This study was performed to understand the ovipositional characteristics and to develop the standardized forecasting method. Oviposition by Ricanula sp. was abundant in tree than in bush, adult laid eggs on new inner twigs and then covered with wax compound. Total no. of oviposited egg-mass was 10 to 318, and that of on new twig was 5 to 185 per tree, with different to host trees. Thickness of oviposited twigs were done within 2 ~ 5.5 mm and the height was mostly founded with range of 1 to 2 m, founded with highest height over 3 m. Oviposited no. of egg-mass within 30 cm twig was appeared differently from 2 to 7 every host. From based on this investigated result, we provide this for standardized forecasting method. This pest will need to control when egg-mass will occur over 2 at new twig, within 30 cm from the tip, set as total 25 point/ 5 plants (5 point per plant).

The aim of this study was to carry out the comparative analysis of the brushing force following various brushing techniques bytoothbrush mounted pressure sensing unit. The study group consisted of 10 dental hygienist participants. The brushing forces (on buccal area of each first molar) were monitored on 8 different kinds of brushing techniques; Fones, Bass, Rolling, Scrub, Charters, stillman, Modified bass and Modified stillman. In Bass, Charters, Fones and Scrub method, force distributions showed a small gap of maximum and minimum value (Max/Min) while a big difference was noted in Modified bass, Modified stillman, Rolling and Stillman methods. Especially, the biggest difference of Max/Min value was observed in the area of lower left first molar. In conclusion, highly delicate manual skill is needed in showing big error range of force distribution. It means that careful force needs to be focused during toothbrushing instruction when a delicate manual skill was carried out.

The spotted-wing drosophila (SWD), Drosophila suzukii (Diptera: Drosophilidae), was originally observed in a few Asian countries, but is now found even in North America and Europe. Genetic information on geographic variation and relationship may broaden our understanding of origin and migration. As a first step, in this study, a portion of mitochondrial COI gene was sequenced to understand genetic relationship and diversity in Korea. Sequencing of 104 individuals provided 57 haplotypes, with the maximum sequence divergence of 1.5%, suggesting high haplotype diversity and moderate sequence divergence. Comparison to GenBankregistered D. suzukii haplotypes (possibly from Spain, Portugal, USA) has shown 100% sequence identity to most of the haplotypes found in this study, but two USA sequences were found to be independent haplotypes, with the sequence divergence ranging from 0.5% ~ 1.4% from our samples in the 553-bp comparison. Phylogenetically, no separable group was found, but, population genetically, the only Chinese population, Sandong, was significantly differentiated (p < 0.05) from all Korean populations, without sharing any haplotype. Among 28 pairwise comparisons of Korean populations only two comparisons showed a significant genetic differentiation, indicating that no population in Korea is completely isolated. Geographically, one haplotype (SWDBA06) was relatively widespread (five among nine localities) and a few haplotypes were found in more than one locality, but most haplotypes were restricted in a locality as a single individual. Overall, high rate of per generation female migration (Nm = 0.75 ~ infinite) and low level of geographic separation (FST=0~0.40) among localities were characteristic. Current data is limited mainly to Korean localities, thus, an expanded study may provide further scrutinized analysis for the fly.

Spider community was surveyed at Nakdonggang River which is the longest river (525 km and 23,384 km2) located East-south part of Korea. Nakdonggang river is developing for water management and environmental conservation including restoration of river ecosystem under “The Four-River Restoration Project”. Sampling was made 3 occasions from June to August in 2009. Eight survey sites were selected from both sides of river in Andong, Sangju, Gumi and Seongju prefectures. Spiders were collected with sweep net for on the plants above ground and pitfall traps for on the ground. A total of 94 species of 71 genera in 24 families were identified from 805 collected spiders. Species richness was high in Salticidae (18 species, 19.1%), Araneidae (12 species, 12.8%), Lycosidae (9 species, 9.6%) in order. Wandering spiders (55 species occupied 58.5%, 493 individuals occupied 61.2%) were higher than webbing spiders (39 species occupied 41.5%, 493 individuals occupied 61.2%) in species richness and abundance. Dominant species were Tetragnatha praedonia (Tetragnathidae) and Neoscona adianta (Araneidae) in webbing spiders, and Anahita fauna (Ctenidae), Pirata procurvus (Lycosidae) and Gnaphosa kompirensis (Gnaphosidae) in wandering spiders with abundance. Collectively, most spiders collected from this study were water preferring spiders and showed different community structure compared to other reports from terrestrial spider fauna. Present result will bevaluable to compare the effect of development on the arthropod fauna after completion of the project.

Hipparchia autonoe belongs to the family Nymphalidae (Lepidoptera) and is designated as an endangered insect and national monument in Korea. It only inhabits a very restricted area on Mt. Halla but is widely distributed in several Asian countries including Mongolia. A previous study conducted to understand the genetic relationship between Mt. Halla and Mongolian H. autonoe for conservation purposes suffered from a limited number of samples. Therefore, we sequenced the DNA barcode region of an additional 36 H. autonoe individuals, combined them with previous data from 19 individuals, and performed phylogenetic and population genetic analyses. Furthermore, the internal transcribed spacer 2 (ITS2) region was also sequenced from the 36 samples as a nuclear DNA marker. The existence of independent haplotypes, sequence types, and significant FST estimates (p < 0.05) between Mt. Halla and Mongolian populations indicated hampered gene flow between the populations. Nevertheless, an absence of a reciprocal monophyletic group in Mt. Halla and Mongolian populations by cytochrome oxidase subunit I gene- and ITS2-based phylogeny suggests that the genetic isolation of the Mt. Halla population from the Mongolian populations seemed not large enough to consider them independent genetic entities.

In the present study, the 17,694-bp long complete mitochondrial genome (mitogenome) of the dwarf honey bee, Apis florea (Hymenoptera: Apidae), is described with an emphasis on the noteworthy triplicated tRNAser(AGN) region and an extraordinary long A+T-rich region with repeat regions. The gene arrangement of A. florea mitogenome is identical to that of A. mellifera, but has triplicated tRNASer(AGN), each of which contains the precedent 44 bp-long and following another 64 bp-long repeats plus one complete first repeat abutting to tRNAMet. A total of 1,610-bp long two repeat regions in 1,987 bp-long A+T-rich region is composed of nearly identical 141 ~ 219-bp long five tandem repeats and 50 ~ 52-bp long 12 tandem repeats that are encompassed by three non-repeat sequences. One of the common interpretations for such repeat sequence is slipped-strand mispairing and unequal crossing-over events during DNA replication.

This study was conducted to estimate the control thresholds (CTs) at different larval densities of Oides decempunctatus Billberg (Coleoptera: Chrysomelidae) of Campbell early in the vineyard and investigated life cycle. Each stage of O. decempunctatus was sampled 18 times from May to September in 2010~2012. The seasonal occurrence of O. decempunctatus showed the highest peak in mid-late June and mid-late August. Overwintered O. decempunctatus's eggs were hatched from late May to early June. Larva period was from late May to mid July and adults appeared in mid July. The percentage of leaf damage (Y) of Campbell early inoculated by different densities of O. decempunctatus (X, no. of larvae/fruiting mother branch) for six weeks was estimated by Y= 0.498X+2.041 (R2=0.988) during vegetation period. The decreasing rate of soluble solid (Y) after grape harvest of Campbell early damaged by different densities of O. decempunctatus (X) was estimated by Y= - 0.046X+15.3 (R2=0.8543). Based of the relationships between the densities of O. decempunctatus larvae and the index of reducing soluble solid of Campbell early, the number of larvae (2nd to 3rd instar) which decreased less than 15°Bx loss of soluble solid was determined as the injury level of 7/fruiting mother branch.

The black-veined white, Aporia crataegi (Lepidoptera: Papilionoidea), is nearly extinct in South Korea, although substantial numbers of dried specimens are available. One of the common practices for such species is to launch re-introduction program after proper amount of genetic information are analyzed from donor and donee populations. In this study, we sequenced complete mitochondrial genome (mitogenome) of A. crataegi to design species-specific primers for subsequent population works and to further understand the mitogenome evolution in lepodiopteran Papilionoidea. The 15,140-bp long A. crataegi mitogenome that has typical sets of 37 genes is smallest among true butterfly species with overall slightly smaller size in genes and regions throughout the genome. Arrangement of the genome is identical to those of other lepidopteran mitogenomes, in which tRNA cluster located between the A+T-rich region and ND2 gene is translocated into tRNAMet, tRNAIle, and tRNAGln from ancestral arrangement, tRNAIle, and tRNAGln, tRNAMet. The A/T content of the genome at 81.3% is the highest in Pieridae, but lower than that of lycaenid species (81.7% ~ 82.7%) The high A/T content in the genome is also reflected in codon usage, accounting for 41.69% of A/T-composed codons (TTA, ATT, TTT, and ATA). Unlikely the diversified or modified usage of anticodon for tRNASer(AGN) the species of Pieridae including A. crataegi all unanimously have GCT that has been hypothesized as ancestral for Lepidoptera. A total of 111 bp of non-coding sequences are dispersed in 13 regions, ranging in size from 1–49 bp. Among them relatively longer ones (≥ 16 bp) all have relatively higher sequence identity to other regions of the genome, suggesting partial duplication of the sequences during A. crataegi evolution. As has been reported in some species of Lepidoptera, the A. crataegi A+T-region also has typically found conserved sequences (e.g., poly-T stretch, ATAGA motif, ATTTA element, microsatellite-like A/T sequence, and poly-A stretch) and one tRNA-like sequence, and this feature was commonly found in true butterfly species.

Al t hough substance P(SP) , a potent pro- inflammatory peptide, is involved in inflammation and immune responses‘ t he eff'ect of SP on t he expression of macrophage inflammatory protein 3a (MIP- 3α CCL20) in periodontal liga ment(PDL) cell s a re unknown, Equally as enigmatic is the link between SP, t he stress protein heme oxygenase- l(HO-l) ‘ and CCL20 procluction, We investigated whether SP induces the release of chemokine CCL20 from immortal ized PDL(IPDL) ceJJ s‘ and fur ther c l a꺼 SP mediated pathways, We also examined the relationship between HO-l a ncl CCL20 by t reating PDL cells with SP, Incubating IPDL cells with SP increased expression of CCL20 mRNA a nd CCL20 protein in a dose-time dependent manner Highly selective p38 and ERKl/2 inhibitors abrogated SP-induced expression of CCL20 in IPDL cell s, SP is a lso responsible for ini t iating phosphorylation of I/C B, degradation of Iκ B‘ ancl activat ion of NF'-/C B, SP induced expression of HO-l in both a concentration- and time-dependent man nel ‘ and CCL20 refl ected s imilar patterns, The inductive effects o[ SP on HO- l and CCL20 wer e enhanced by HO- j inducer hemin and the membrane-permeable cGMP analog 8-bromo-cGMP, Conversely, this pathway was inJübited by t he 1-10난 inhi bitor zinc protoporphyrin IX(ZnPP IX) and the selective inl뼈itor of guanylate cyc1ase‘ lH-[l , 2, 4Joxad iazole[4‘ 3-aJquinoxal in-l-one (ODQ) , We report herein the pathway that connects SP along with other modulators 。f neuroimmunoregulationto the induction of HO-l and t he inflammatory mediator MIP-3a /CCL20 in IPDL cell s‘ which play an important role in the development 01' periodontitis or inflamrnation during orthodontic tooth movem

뻐ny studìes have shown the anti-proli ferative effects of irondeprivation on cancer cell s‘ but the effects 01' iron-chelators on oral cancer have not been clearly elucidated , To investigate the effects of an iron chelato r, desferrioxamine( 01"O).on the growth of ilIllTIortali zed human o1'al ke ratinocytes(IHOK), primary oraJ cancer cel ls(HN4)‘ metastatic oral cancer cell s(HN12) , and human skin keratìnocytes(HaCaT) in the MTr assay, three-dimensional(3D) raft cul tmes, Western blott ing, cell cycJ e analysis‘ nuclear staining‘ and cytochrome c expression for apoptosis s ig naling pathway were used OFO inhibited the growth of immortalized IHOK and HaCaT and mal ignant HN4 and HN12 keratinocytes in a time- and dose-dependent manner according to the MTT assay, The 3D organotypic cu l tu re also revealed that OF'O-treated cells showed less epithelial maturation, less surface keratinizati on‘ and de creased epithelia l thickness, The major mechanìsm of growth inhìbition with the micromolar 0 1"0 treatment was by the induction of apoptosis‘ which was supported by nuclear OAPI staining, ONA fragmentation analysis, and J10w cytometric analysis for sub-Gl phase ar rest and Annexin V-1"ITC stainìng, Furthermore‘ Bax expression in creased together with p53 and p21WAF1!CIPl, whìle the Bcl-2 expression decreased in the immortalized and malig nant keratinocytes treated with 01"0 , Time-dependent cytochrome c from mitochondria was observed in D1"O-treated [l-IOK and 0 1'머 cancel‘ ceJJ s, and was accompanied by the activation of caspase-3 in IHOK cells. These resu lts demonstrate that 0 1"0 has growth inhibitory effects on immortalized and malignant oral keratinocyLes Lhrough the induction of apoptosis and suggest that further evaluation of OFO as a potcntial thcrapcutic agent for human oral precancerous and cancerous lesions is warranted

A baffle system for an airglow photometer, which will be on board the Korea Sounding Rocket-III(KSR-III), has been designed to suppress strong solar scattered lights from the atmosphere below the earth limb. Basic principles for designing a baffle system, such as determination of baffle dimensions, arrangement of vanes inside a baffle tube, and coating of surfaces, have been reviewed from the literature. By considering the constraints of the payload size of the KSR-III and the incident angle of solar light scattered from the earth limb, we first determined dimensions of a two-stage baffle tube for the airglow photometer. We then calculated positions and heights of vanes to prohibit diffusely reflected lights inside the baffle tube from entering into the photometer. In order to evaluate performance of the designed baffle system, we have developed a ray tracing program using a Monte Carlo method. The program computed attenuation factors of the baffle system on the order of 10 -6 for angles larger than 10°, which satisfies the requirements of the KSR-III airglow experiment. We have also measured the attenuation factors for an engineering model of the baffle system with a simple collimating beam apparatus, and confirmed the attenuation factors up to about 10 -4. Limitation of the apparatus does not allow to make more accurate measurements of the attenuation factors.

지금까지 추진해 오던 제외지 중심의 하천복원에서 벗어나 최근에는 제내지까지 복원하고자 하는 노력이 시도되고 있다. 이의 일환으로 구하도의 복원이 추진되고 있으나 이로 인한 수리적 연결성 및 생태적 연결성 향상을 정량적으로 평가할 적합한 모형의 개발은 미진한 상태이다. 본 연구에서는 구하도 복원을 통한 생태적 연결성 회복을 평가할 수 있는 격자기반의 수리해석 모형을 개발하였다. 본 모형의 적용성을 검토하기 위하여 노탑리 일원의 청미천 하천복원 사업지를 대상으로 수리생태적 연결성을 평가하였다. 본 모형으로 수리 및 생태적 특성의 시 ․ 공간적 분포를 신속하고 간단하게 해석할 수 있었으며 향후 수리적 생태적 연결성을 평가하는 적절한 도구로 활용될 수 있을 것으로 판단된다.

Magnetic flux ropes, often observed during intervals of interplanetary coronal mass ejections, have long been recognized to be critical in space weather. In this work, we focus on magnetic flux rope structure but on a much smaller scale, and not necessarily related to interplanetary coronal mass ejections. Using near-Earth solar wind advanced composition explorer (ACE) observations from 1998 to 2016, we identified a total of 309 small-scale magnetic flux ropes (SMFRs). We compared the characteristics of identified SMFR events with those of normal magnetic cloud (MC) events available from the existing literature. First, most of the MCs and SMFRs have similar values of accompanying solar wind speed and proton densities. However, the average magnetic field intensity of SMFRs is weaker (~7.4 nT) than that of MCs (~10.6 nT). Also, the average duration time and expansion speed of SMFRs are ~2.5 hr and 2.6 km/s, respectively, both of which are smaller by a factor of ~10 than those of MCs. In addition, we examined the geoeffectiveness of SMFR events by checking their correlation with magnetic storms and substorms. Based on the criteria Sym-H < -50 nT (for identification of storm occurrence) and AL < -200 nT (for identification of substorm occurrence), we found that for 88 SMFR events (corresponding to 28.5 % of the total SMFR events), substorms occurred after the impact of SMFRs, implying a possible triggering of substorms by SMFRs. In contrast, we found only two SMFRs that triggered storms. We emphasize that, based on a much larger database than used in previous studies, all these previously known features are now firmly confirmed by the current work. Accordingly, the results emphasize the significance of SMFRs from the viewpoint of possible triggering of substorms.

베트남 북부 산악지형에 거주하는 소수 부족민들의 생계개선이 베트남 정부의 정책적인 지원 사업에도 불구하고 현 재까지 뚜렷한 성과를 내지 못하는 것으로 알려져 왔다. 특히 지속가능한 개발 및 인적자원개발을 목표로 하고 있는 베트남 경제사회개발정책(2011~2020)의 하위전략인 신농촌 개발정책(New Rural Development)에 의한 사업들이 적절히 수행되고 있는지 의문이 대두되었다. 한편, 베트남 라오까이성 행복프로그램은 한국 코이카 재원으로 새마을운동 경험과 정신을 바탕으로 설계되었으며 심각한 빈곤상태에 있는 성내 8개 소수부족민 마을에 마을특성과 주민 의견이 반영된 개발계획을 수립하고 계획실행의 주체인 마을주민들과 현장 공무원에게 다양한 훈련 사업들을 제공하였다. 본 연구는 생계개선에 대한 이론 고찰 과 함께 한국 및 베트남의 농촌개발 경험사례 분석을 바탕으로 프로그램의 다양한 역량강화 사업들이 8개 소수부족 주민들 의 의식변화와 생계자산 향상에 어떠한 영향을 끼쳤는지 알아보았다. 본 연구결과는 프로그램에서 제공한 다양한 역량강화 훈 련들이 주민의식의 긍정적인 변화와 소수 부족민들의 생계자산에 대하여 상당한 만족도를 가져왔는바, 신농촌 개발정책은 직접자재 위주의 지원을 줄이는 대신 주민들의 자신감 고취를 위한 주민의식 교육과 주민들의 생계활동 능력향상을 위한 다양한 훈련 사업을 확대해야 함을 보여준다.