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        검색결과 37

        21.
        2013.09 KCI 등재 서비스 종료(열람 제한)
        KAS360-22 종자에 1998년 250 Gy의 감마선을 조사하였다. 그 후대에서 미이라병에 저항성을 가지는 계통을 선발・육성하여, ‘원율’로 명명하고 2009년에 최종적으로 품종보호권을 출원 신청하여 2012년 등록 완료하였다. 원품종 KAS360-22의 종피색이 노란색인데 반하여 신품종 ‘원율’은 갈색을 나타냈다. 100립중은 재래종 KAS360-22이 17.2 g, ‘원율’은 27.5 g으로 종자무게는 증가하였으며, ‘일품검정콩’(27.4g)과는 별다른 차이를 보이지 않았다. 신품종 ‘원율’의 10a 당 수량은 미이라병 발병 조건에서 233.3 kg으로 재래종 KAS360-22 (24.9 kg)에 비교하여 10.6배 가량 높았고, 대조구 ‘일품검정콩’(121.4 kg)에 비교하여도 1.9배 가량 높아 밥밑용 콩으로 안정적인 수량 확보가 가능할 것으로 사료된다.
        22.
        2013.07 서비스 종료(열람 제한)
        To determine the expression levels of genes related to the salt stress response in rice, gene expression profiles were investigated through microarray analysis using the rice mutant line Till-II-877. There were no significant changes in physiological response under salt stress of the mutant increased less than that in the WT. The intensity of gene expression was analyzed and compared between the wild type and mutant lines using a microarray. Among the most significantly affected pathways, α-linolenic acid metabolism and linoleic acid metabolism (in lipid metabolism), fructose and mannose metabolism and glycolysis-gluconeogenesis (in carbohydrate metabolism), cysteine and methionine metabolism (in amino acid metabolism), and carbon fixation (in the energy metabolism of photosynthetic organisms) showed changes in gene expression levels under salt stress. These results further our understanding of the effects of salt stress in rice and may aid in the development of salt-tolerant rice cultivars.
        23.
        2013.07 서비스 종료(열람 제한)
        Soluble sugar content in soybean seed is an important quality attribute for soyfood and feed. Usually, soluble sugars comprise 6 to 17% of total dry wt. in mature soybean seeds. In this study, 414 soybean mutant lines induced by gamma-ray were screened by colormetric assay, FACE (Fluorophore-assisted carbohydrate electrophoresis), and GC-MS to identify the change of soluble sugar contents. Among 414 soybean mutant lines, 12 mutant lines derived from three different soybean cultivars (Hwanggum, Paldal, and Bangsa) showed higher level of soluble sugar content compared to their original cultivars. However, 5 mutant lines derived from soybean landrace KAS 636-15 showed lower level in the colormetric assay. In FACE, 17 soybean mutant lines selected by colormetric assay also showed different band intensity compared with their original cultivars. However, there were no different soluble sugar patterns between soybean original cultivars and mutant lines. Finally, the variations of soluble sugar content in 17 soybean mutant lines were confirmed by using GC-MS. These mutant lines will be used for genetic study to find mutations of genes related soluble sugar biosynthesis.
        24.
        2012.07 서비스 종료(열람 제한)
        The protein in soybean seeds accounts for approximately 40% of the dry seed weight. Two major storage proteins, 7S and 11S, constitute 70-80% of the total storage proteins in the seeds. In this study, the variation of total soluble protein extracts from 1152 soybean landraces that have been collected from South Korea were studied using high-throughput screening method with HT Protein Express Labchip (Caliper Life Sciences, Inc.). Seven distinct protein band patterns - four protein sub-units of 11S and three sub-units of 7S, were taken into account and their presence or absence were analyzed. Among the 1152 landraces, 525 genotypes were identified as lacking lipoxygenase, 255 lacking α1 subunit, 680 lacking α subunit, 169 lacking β subunit, 140 lacking acidic, 114 lacking Kunitz Trypsin Inhibitor (KTi) and 199 lacking basic protein patterns. The high-throughput protein analysis is helpful in screening a large number of populations with less time and minimum labor. The selected genotypes with low amounts or lacking of anti-nutritional factors such as trypsin inhibitor, lipoxygenase and α subunit would be used for future breeding purpose of quality improvement in soybean protein.
        27.
        2010.06 KCI 등재 서비스 종료(열람 제한)
        재래종 서리태는 밥밑용 콩으로 한국인에게 선호되고 있으나, 숙기가 느려 수량성이 떨어지고 콩알이 커서 청소년들에게는 기피 경향이 있다. 서리태의 특성을 개량하고자 1994년 수집한 서리태 종자에 감마선 250 Gy를 조사하여 돌연변이 육종을 시도하였다. 그 후대에서 숙기가 빠르고 수량성이 높으며 콩알 크기가 작아진 유망 돌연변이 계통을 선발하여 ‘조생서리’로 명명하고, 2005년도에 품종보호권 및 국가품종목록등재 신청을 하였으며, 2008년도에 등록을
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