Frequent surveys and monitoring were conducted in the Southern part of Bangladesh to detect and identify the plant pathogenic virus that infecting agriculturally important vegetables during 2017-2018. A total of 28 fields of the survey area were closely monitored. The findings indicated that 21.94% of the plants developed typical virus disease like symptomps in the field. However, 28.21% infected plants were found in Patuakhali followed by Satkhira (23.11%), Khulna (19.33%) and Barguna (17.12%). The symptoms were mosaic, vein clearing, chlorosis, curling and ringspot. Twenty samples from the collections were randomly chosen on the basis of symptoms and subjected to Enzyme-linked Immunosorbent Assay (ELISA) with the antiserum and symptomalogy were used for detection. Eight viruses namely Cucumber mosaic virus (CMV), Okra yellow vein clearing mosaic virus (OYVCMV), Mungbean yellow mosaic virus (MYMV), Tomato yellow leaf curl virus (TYLCV), Pepper mottle virus (PMV), Papaya ringspot virus (PRSV), Watermelon mosaic virus (WMV) and Zucchini yellow mosaic virus (ZYMV) were detected on cucumber, okra, brinjal, mungbean, tomato, pepper, papaya, watermelon and pumpkin respectively.