This study presents a rapid and sequential radiochemical separation method for Pu and Am isotopes in radioactive waste samples from the nuclear power plant with anion exchange resin and TRU resin. After radionuclides were leached from the radioactive waste samples with concentrated HCl and HNO3, the sample was allowed to evaporate to dryness after filtering the leaching solution with 0.45 micron filter. The Pu isotopes were separated in HNO3 medium with anion exchange resin. For leaching solution passed through anion exchange column, the Am isotopes were separated with TRU resin. The purified Pu and Am isotopes were measured by alpha spectrometer, respectively, after micro-precipitation of neodymium. The sequential radiochemical separation of Pu and Am isotopes in radioactive waste samples using anion exchange resin and TRU resin was validated with ICP-MS system.

This study presents a rapid and quantitative radiochemical separation method for Nb isotopes in radioactive waste samples from the nuclear power plant with anion exchange resin after Fe coprecipitation. After radionuclides were leached from the radioactive waste samples with concentrated HCl and HNO3, the Nb isotopes were coprecipitated with Fe after filtering the leaching solution with 0.45 micron HA filter, while the Sr, Tc and Ni isotopes were in the solution. The Nb isotopes were separated in HCl medium with anion exchange resin. The purified Nb isotopes were measured using a low level liquid scintillation counter after installing quenching curve with standard Nb-94 isotopes. The separation method for Nb isotopes investigated in this study was applied to neutron dosimeter samples from the nuclear power plant after validating the Nb activity concentration with gamma spectrometry system.

Recently, as the demand for a non-contact liquid crystal alignment method capable of improving viewing angle characteristics has spread throughout the industry, various non-contact liquid crystal alignment methods, including conventional UV light alignment, are being actively studied. In the case of UV light alignment, it is currently applied to mass production in many fields and shows relatively excellent initial characteristics, but there is a problem of display quality deterioration over time. In this study, among these non-contact liquid crystal alignment methods, the liquid crystal is oriented by quantitatively irradiating an ion beam onto the SiOF inorganic film, which has excellent initial characteristics and does not cause deterioration in quality over time., the electro-optical properties were evaluated by manufacturing a commercial-level IPS (In-Plane Switching) liquid crystal cell. In particular, in the case of such inorganic film orientation, it is common to have many problems with orientation stability, but the evaluation cell manufactured by the method proposed in this study is capable of maintaining a uniform orientation without losing orientation even after heat treatment at a high temperature of 200°C. could be observed.

Cymbidium is one of the most popular and economically important species cultivated as a commercial ornamental crop. The objectives of this study were to determine the appropriate electrical conductivity (EC) treatments of nutrient solution, which gives the highest spike production and quality. Three-year-old Cymbidium ‘Lovely Smile’ plants were grown in the environmentally controlled Information and Communication Technology (ICT) smart greenhouse at Seoul Women’s University. The EC of the nutrient solution was changed in three distinct stages: vegetative, flower initiation, and flower development. The EC treatments were 1-0-1 (dS·m-1, EC101), 1-1-1 (dS·m-1, EC111), 2-1-2 (dS·m-1, EC212), 2-2-2 (dS·m-1, EC222), 3-2-3 (dS·m-1, EC323), 3-3-3 (dS·m-1, EC333) and the pH was adjusted to 6.0–6.5. Pseudobulb diameter increased in the plants treated with EC 101 and EC111 compared to the plants treated with EC 2.0–3.0 dS·m-1 at the reproductive stage 28 weeks after nutrient solution treatment. Flower spike production per pot and pseudobulb showed the highest values in the plants treated with EC111 of 3.3 and 1.4, respectively. Flower spikes length was the highest in the plants treated with EC 1.0 dS·m-1 and stem thickness, number of flowers, and fresh weight were the largest in the plants with EC 1.0 dS·m-1 among the EC treatments. Flower spikes had the worst quality (e.g., plant growth and flowering quality) in the plants treated with EC 3.0 dS·m-1 among the EC treatments. Floral bud and flower development took place 1–2 weeks earlier in the plants treated with EC 101, 111, and 212 than the other treatments. Flower diameter showed the highest values in the plants treated with EC 1.0 dS·m-1 among the EC treatments and flower color showed higher L* and b* values and lower a* values in the plants treated with EC 3.0 dS·m-1 compared to EC 1.0 and 2.0 dS･m-1. Nutrient solution of EC 1.0 dS·m-1 (EC111) can be recommended to improve flower spike quality and advanced flower development of Cymbidium.

Naturally occurring left ventricular hyperplasia is a rare but lethal disease. There are very few reports of this cardiac disease in captive nonhuman primates. In a colony of Macaca mulatta (Rhesus monkey) at California National Primate Research Center, a large number of rhesus macaques were diagnosed by autopsy with naturally occurring left ventricular hypertrophy without obvious underlying diseases over a 22-year period. The confirmatory diagnosis of ventricular hypertrophy was based on findings of notable left ventricular concentric hypertrophy with reduced left ventricular lumen, which is very similar to human ventricular hypertrophy cases. This report discusses an 11-year-old Macaca fascicularis monkey (Cynomolgus monkey, crab-eating macaque), weighing 2.95 kg, that was presented for enrollment in a pharmacokinetic (PK) study. During the PK experiment, the monkey died following a sudden decrease in percutaneous oxygen saturation and heart rate. Gross and histological examinations of the heart were performed. On gross pathology, the left ventricular wall was thickened, and the chamber lumen was reduced. In histopathological examination using hematoxylin- eosin and Masson-trichrome stains, fibrosis and myocyte disarray were not observed, but an increased cell density, compared to the normal heart, was confirmed. The autopsy results confirmed left ventricular hyperplasia as the major cause of death.

Porcine pleuropneumonia caused by Actinobacillus pleuropneumoniae is a highly contagious disease that leads to enormous economic losses in pig industry, worldwide. Of the many virulence factors produced by the causative bacterium, ApxA exotoxins have been considered as the most important contributor to the disease. The toxins are classified into four different types; ApxIA, ApxIIA, ApxIIIA and ApxIVA. Uniquely, ApxIVA is expressed across all serotypes of A. pleuropneumoniae only during in vivo infection in pigs. Active research focusing on resolving the precious roles and mechanisms of the toxins is still at its primitive stage. In this study, we report the development of monoclonal antibodies against the two major antigenic epitopes that were characterized in our previous study incorporating the in silico predictions and protein modeling analyses. Recombinant proteins of the selected epitopes were expressed and purified after molecular cloning of the corresponding partial genes in E. coli expression system. Subsequently, we generated hybridomas with lymphoid cells from the rats immunized with the recombinantly expressed proteins of Apx. Consequently, hybridomas exhibiting strong productivity of the monoclonal antibodies were selected for downstream verifications that tested for reactivity and specificity using Western blot and ELISA. Our results strongly suggest the potential application of the monoclonal antibodies developed in this study as useful reagents to further elaborate the mechanism of the A. pleuropneumoniae infection in pig.

The detection of Mycobacterium bovis (M. bovis) in environmental samples with precision is imperative to control bovine tuberculosis (bTB) infections at the herd level, as residual M. bovis remains one of the major causes of recurring infections. In this study, a nested PCR method for the detection of M. bovis in environmental samples was applied to identify potential environmental reservoirs of the bacterium. A set of 200 environmental samples (167 fecal samples and 33 water samples) from 39 herds with a history of bTB outbreak was analyzed using a nested PCR method to detect residual M. bovis. Amplicon libraries of the IS6110 target gene fragment were amplified from M. bovis DNA using two established primer sets. A positive nested PCR result was observed in 69.5% of fecal samples and 66.7% of water samples, thus showing that residual M. bovis was present in the environmental samples of bTB-positive herds in a high proportion. This study is the first to demonstrate high levels of M. bovis DNA in environmental samples and to show that environmental reservoirs of this pathogen contribute to recurring outbreaks of bTB. Environmental monitoring of herds in which bTB outbreaks have occurred with high sensitivity and specificity is expected to help prevent the recurrence of potential bTB disease and improve the herd environment.

In this study, we studied the method of using general architectural glass instead of using the existing acrylic material for high luminance flat lighting. The flat panel lighting used the side illumination method to increase the ease of installation and aesthetic satisfaction. In general, glass has an amorphous structure with a lower angle of refraction than acrylic, so it is not suitable for use in flat panel lighting as a light guide, but in this study, the role of light distribution characteristics and diffusion patterns in the case of using such a glass light guide. Quantitative simulations were conducted to confirm new possibilities. In the simulation, the backlight estimation method was used, and about 10,000,000 rays were placed within a unit area in order to obtain a result similar to the real thing. As a result of the simulation, the geometry of the diffusion pattern could be specified, and the value of the geometry could be quantified using the ratio of the diameter and height of the pattern. As a result of the calculation, it was found that the maximum amount of light was generated around 75 degrees by quantitatively calculating the ratio and the outgoing light angle at which the maximum value of the outgoing light occurred between 05 and 1.0. As a result of these studies, it was confirmed that it is possible to use ordinary glass at the same time as a transparent window and light-emitting lighting at night.

한국 땅나리와 섬말나리의 수요는 최근까지도 늘어났지만 종자 상태에서 개화에 이르기까지 확률은 낮은 편으로 자생지에서의 개체수는 해마다 감소하였다. 본 연구는 땅나리(Lilium callosum Siebold & Zucc.)와 섬말나리(L. hansonii Leichtlin ex D.D.T. Moore)의 대량증식을 도모하기 위하여 종자 발아에 유리한 주·야간 온도 조건과 적합한 GA3 농도를 알아보는 것을 목적으로 수행되었다. 땅나리 종자는 2017년 10월 24일 강원도 춘천히 둔산면 원창리(37°47’10.00”N, 127°44’31.00”E), 섬말나리 종자는 2017년 10월 2일 울릉도 울릉군 북면 나리 (37°31’27.06”N, 130°52’25.08”E)에서 채종하였다. 땅나리와 섬 말나리 종자들을 주·야간 15/5, 20/10, 25/15, 20/20°C의 환경 조절상에서 처리구 당 30립씩 3반복으로 치상하여 온도 처리 실험을 진행한 후, 발아율 결과가 가장 우세했던 온도 조건의 환경조절상에서 섬말나리 GA3 처리 실험을 추가로 진행하였다. 온도 처리 실험결과 땅나리는 20/20°C의 온도 조건에서 발아율이 95.0%로 가장 높았으며 평균발아소요일수 또한 10.1일로 가장 낮았다. 섬말나리는 20/20°C의 온도 조건에서 발아율이 78.8%로 가장 높았으며 평균발아소요일수 또한 40.1일로 가장 낮았다. 이를 토대로 20/20°C에서 실시한 섬말나리 GA3 처리 실험은 증류수를 대조구로하여 200, 400, 800mg･L-1의 농도로 상온에서 24시간 침지한 후 처리구 당 20립씩 3반복으로 치상하였다. 그 결과 대조구에서 81.6%로 가장 높은 발아율을 나타냈으며 평균발아소요일수는 200mg･L-1처리구에서 37.1일로 가장 낮았다. 이러한 실험 결과를 바탕으로 땅나리와 섬말나리의 주·야간 발아 최적 온도는 20/20°C이며 GA3 24 시간 처리는 섬말나리 종자 발아에 효과가 없는 것으로 판단 되었다.

목적 : 여러 분자량의 polyethylene glycols(PEGs)을 화학적 공유결합으로 하이드로겔 콘택트렌즈 표면에 고정 시켰다. PEG의 도입이 렌즈의 표면 습윤성, 단백질 흡착성, 광투과율 등에 미치는 영향을 PEG의 길이 혹은 PEG 의 적용여부 등에 초점을 맞추어 분석하는데 실험 목적이 있다. 방법 : PEG에 Jones oxidation 반응을 통해 알코올기를 카르복실 작용기로 변형시켰고, 하이드로겔 콘택트렌즈 표면에 화학적으로 결합시켰다. 역상 고성능 크로마토그래피와 단백질 표준검량선을 이용하여 제조된 렌즈들에 흡착된 단백질을 정량하였다. 결과 : PEG가 개질된 하이드로젤 콘택트렌즈는 우수한 광투과율과 표면 습윤성을 보였고 이는 상업적으로 이용가능한 수치이다. 단백질 흡착 실험 결과를 살펴보면, 보단 긴 PEG 사슬이 적용된 하이드로겔 콘택트렌즈는 표면 친수성이 더 우수하기 때문에 단백질 흡착량이 더욱 감소하였다. 결론 : 본 연구에서는 PEG가 표면-개질된 하이드로겔 콘택트렌즈를 제조하고 이들의 물성을 조사하였다. PEG 각 적용된 렌즈는 90% 이상의 광투과율과 개선된 표면 습윤성을 보여주었다. 특히, 보다 긴 PEG2000이 적용된 렌즈에는 PEG가 적용되지 않은 대조군이나 짧은 PEG164가 적용된 렌즈 보다 단백질의 흡착이 크게 감소되었다. PEG가 표면에 적용된 하이드로겔의 제조는 안의료용 바이오소재 뿐만 아니라 단백질-비흡착 기기의 개발에 큰 역할을 할 것으로 기대된다.

본 연구에서는 원자력 사고 또는 방사선 비상 시 지표면에 침적될 수 있는 감마선방출 핵종의 방사능을 신속하게 평가하기 위해 이용될 수 있는 NaI(Tl), LaBr3(Ce) 및 CeBr3 섬광검출기의 성능을 비교 평가하였다. 검출성능은 최소검출가능방사능 (MDA, Minimum Detectable Activity)을 통해 평가하였으며, 각 검출기의 지표면 침적 감마선방출 핵종에 대한 검출효율은 수학적 모델링과 점선원을 이용하여 반실험적으로 산출하였다. MDA 평가를 위한 백그라운드 감마선에너지스펙트럼은 비교적 넓고 평탄한 초지에서 측정되었으며, 원자력 사고 시 방출될 수 있는 주요 핵종에 대한 각 검출기의 MDA를 산출하였다. 그 결과 일반 환경방사능 준위에서 지표면 침적 감마핵종에 대한 각 검출기의 MDA 크기는 대체로“NaI(Tl)> LaBr3(Ce)> CeBr3”로 평가되었으며, 백그라운드 준위가 유사한 에너지 영역에서는 분해능이 가장 우수한 LaBr3(Ce)에서 최소 값을 보였다. 이는 관심 핵종의 감마선에너지 영역에 대한 각 검출기의 자체 및 측정 환경 백그라운드, 측정 효율, 그리고 에너지 분해능 특성을 바탕으로 비교 분석되었다.

The Al-Zour coastal area, located in southern Kuwait, is a region of concentrated industrial water use, seawater intake, and the outfall of existing power plants. The Al-Zour LNG import facility project is ongoing and there are two issues regarding the seawater temperature in this area that must be considered: variations in water temperature under local meteorology and an increase in water temperature due to the expansion of the thermal discharge of expanded power plant. MIKE 3 model was applied to simulate the water temperature from June to July, based on re-analysis data from the European Centre for Medium-Range Weather Forecasts (ECMWF) and the thermal discharge input from adjacent power plants. The annual water temperatures of two candidate locations of the seawater intake for the Al-Zour LNG re-gasification facility were measured in 2017 and compared to the numerical results. It was determined that the daily seawater temperature is mainly affected by thermal plume dispersion oscillating with the phase of the tidal currents. The regional meteorological conditions such as air temperature and tidal currents, also contributed a great deal to the prediction of seawater temperature.

The aged asphalt binder included in RAP due to the oxidative aging, repeated vehicle load, climate process affects to the recycled asphalt mixture property and performance (stripping, port hole and premature cracking initiation) after paving. The rejuvenator commonly is used to recover the aged binder in hot mix asphalt (HMA) containing RAP; the effect of rejuvenator in HMA had been proven according to many studies for over the past several decades. Also, there are many methods for using RAP in asphalt mixture in aspects of HMA, cold asphalt mixture (CMA) and worm mix asphalt mixture (WMA), and a foamed asphalt mixture is one of them. Employing the foamed asphalt manufacturing technology, the content of RAP in recycled asphalt mixture can be increased more. The objectives of this study are to evaluate of rejuvenator influence on foamed asphalt mixture using 100% RAP based on strength change of test sample and stiffness change of recovered binder from RAP and specimen. As the results, when rejuvenator was added to make foamed asphalt mixture, MS and ITS values decreased clearly as compared with the foamed asphalt mixture without rejuvenator use. The use of rejuvenator up to 6% showed a tendency of the decrease of strength and stability remarkably. The use of rejuvenator over 6% did not decrease the strength and stability. DSR test results, the use of rejuvenator in making a foamed asphalt mixture using 100% RAP showed a recovery effect of the foamed asphalt mixture. And recovered binder from the specimen that was made adding the 6, 12 and 18% rejuvenator showed lower stiffness obviously compared to the recovered binder from RAP adding same dosage of rejuvenator.