본 연구는 벼 화분에 잔류한 네오니코티노이드계 약제가 꿀벌 봉군 내로 유입하여 만성적으로 피해를 주는지 에 대해 실험적으로 검증하고자 한다. 벼 꽃 개화기에 맞춰 논 인근의 세 지역에 각 6개 봉군을 설치하였다. 3개의 지역 중 두 지역은 벼 꽃 개화기 항공 방제 수행지역이고, 1개 지역은 미수행 지역이다. 지역마다 봉군 3개에는 채분기를 설치하여 벌통 내 화분 유입을 차단한 그룹과 미설치 그룹 간 봉세와 꿀벌 면역 및 수명 관련 유전자 발현량을 비교하였다. 약제 방제가 수행된 지역에서 채분기를 설치한 봉군의 봉세는 미설치 봉군보다 상대적으 로 강한 것을 확인하였다. 또한, 약제 처리 지역에서 채분기 설치 봉군에서 채분기를 설치한 봉군의 봉세는 미설치 봉군보다 상대적으로 강한 것을 확인하였다. 또한, 약제 처리 지역에서 채분기 설치 봉군에서 채집된 꿀벌의 면역 및 수명 관련 유전자 발현량이 미설치 그룹과 차이가 있는 것을 확인하였다.

The rate of resistant pest emergence has accelerated due to the continuous use of pesticides. Therefore, it is important to formulate insecticide resistance management measures and effective control methods for pest. Bemisia tabaci, a greenhouse pest, causes direct damage to crops such as growth inhibition and leaf discoloration at all developmental stages except for eggs. It also indirectly damages plants by secreting honeydew, which covers surrounding leaves and fruits, leading to sooty mold development. In this study, eight insecticides with high usage rates, categorized by their mode of action, were selected. Samples of Bemisia tabaci were collected from six regions, and resistance analysis were conducted. The results showed that Flonicamid exhibited a resistance ratio of 8.91 in Sejong, while Pyriproxyfen showed a high resistance ratio of 63.56 in Gunwi. Fluxametamide, Spinetoram, Cyantraniliprole, Dinotefuran, Pyridaben, and Milbemectin displayed resistance ratio ranging from 0.02 to 1.14 in most regions, except for Flonicamid and Pyriproxyfen.

This study was conducted to determine the appropriate seeding dates by verifying the difference in winter survival and productivity of alfalfa according to fall sowing dates in the central area of South Korea. The experiment was conducted for 2 years (2020 and 2021) at the field in the Department of Animal Resources Development, NIAS located in Cheonan. Sowing dates started from September 18 to November 8 with 10 days of intervals during 2020 and 2021; SO1 (September 18), SO2 (September 28), SO3 (October 8), SO4 (October 18), SO5 (October 28), and SO6 (November 8). After sowing, the winter survival rate was measured in the spring of the following year, and the dry matter yield was measured by harvesting at 10% flowering and harvesting five times a year. SO6 failed to winter survival, and SO5 also had a lower winter survival rate than SO1~4 (p<0.05). The average annual dry matter yield of alfalfa linearly decreased with delaying sowing dates (p<0.05). The feed value did not differ in the same year by delaying the sowing date in the same year. These results suggest that sowing date should be started before October 18 to increase winter survival and productivity of alfalfa in the central area of South Korea.

The aim of this study was to investigate dry matter productivity and nitrate nitrogen content in the growth stages of barnyard millet (Echinochloa esculenta) cultivated for feed, which was treated with different nitrogen fertilization levels. An early variety of barnyard millet (cv. Shirohie) was used for the test, and the different treatments with nitrogen fertilizer were as follows: 50% (N-40 kg/ha, T1), 100% (N-80 kg/ha, reference amount, T2), 150% (N-120 kg/ha, T3), 200% (N- 160 kg/ha, T4), 250% (N-200 kg/ha, T5), and 300% (N-240 kg/ha, T6). Sowing was done on May 13, 2021 and plants were harvested for four stage; vegetative stage, elongation stage, heading stage, and milk stage. The length of the millet increased significantly as the amount of nitrogen fertilization increased during the harvest period (p<0.05), but the difference was insignificant during the milk stage (p>0.05). Moreover, barnyard millet dry matter yield increased significantly as the levels of nitrogen fertilization increased (p<0.05), but there was no significant difference in dry matter yield among nitrogen fertilization levels during the heading stage (p>0.05). Chlorophyll also was significantly higher in T5 (250%) at all harvesting times, whereas nitrate nitrogen content was highest at the vegetative stage, gradually decreased as growth progressed, and lowest at the milk stage. Finally, as the nitrogen fertilization levels increased, the nitrate nitrogen content was significantly higher in all treatment groups (p<0.05). Therefore, our results suggest that the most appropriate nitrogen fertilizer levels is between 150%–200%, considering the dry matter yield, feed ingredients and nitrate nitrogen content in barnyard millet for feed.

This study aimed to examine the changes in dry matter yield and growth characteristics of alfalfa (Medicago sativa L.) in response to variations in sowing dates during the autumn season of 2021-22 in a dry paddy field of Chilbo-myeon, Jeongeup-si, Jeollabuk-do. Treatments comprised four sowing dates at 10-day intervals, i.e., October 8, October 18, October 28, and November 8, 2021. The winter survival rate of alfalfa showed a significant difference between different treatments but was at a satisfactory level for all (p<0.05). The winter survival rate for the fourth sowing date, a month later than the first sowing date, was approximately 11.7% lower than that for the first sowing date. The plant height ranged between 82.3–93.1 cm and 60.5–63.7 cm at the first and second harvest, respectively, smaller at the second harvest than at the first harvest. The total dry matter yield of alfalfa was the highest at 13,316 kg/ha for the first sowing date, and the later the sowing date, the lower the dry matter yield. The protein content of alfalfa ranged between 13.6–17.3% in the first harvest, lower than the standard alfalfa protein content of 20% or more. In relative feed value, the first sowing (Oct. 8) was the most significantly higher in the first harvest (p<0.05). These results suggest that the early and mid-October sowing dates are optimum for sowing alfalfa during autumn and result in improved plant growth, dry matter yield, protein content, and winter survival compared to those at later sowing dates. Therefore, dry paddy fields can be safely employed for alfalfa cultivation with sowing dates in early and mid-October during autumn.

Barnyard millet (Echinochloa esculenta) can be processed through soiling, hay, and silage, depending on the weather conditions during harvesting. However, research on barnyard millet is insufficient, and standards for cultivar, seeding density, and fertilizers have not been established. This study was conducted to examine the effects of seeding density and seeding methods on dry matter yields. For this, we used the early-maturing (Shirohie millet) variety of the barnyard millet. The experimental design included different seeding density (10 kg/ha, 15 kg/ha, 20 kg/ha (standard seeding density), 25 kg/ha and 30 kg/ha), and different methods of seeding (drill seeding and broadcast seeding). The seeding date was May 13, 2021, and the harvest date was July 13, 2021. Harvesting was carried out when the heading reached 40 %. Lodging occurred at 5, 9 and 7 at 20, 25 and 30 kg/ha densities in the broadcast seeding, but not in the drill seeding. With decreasing density of seeding, tillage number showed an increasing trend in both drill seeding and broadcast seeding (p>0.05). The plant heights were comparable in both drill seeding and broadcast seeding (p>0.05). The heading stage of barnyard millet was checked July 7 for drill seeding, and, on July 8 for broadcast seeding. It took 62 days, i.e., till July 13 for the heading to reach 40 % of the output. The dry matter yield of barnyard millet was significantly higher at the seeding density of 30 kg/ha, for both the methods of seeding (p<0.05). There was no difference in the chemical composition of grain, based on the seeding method and seeding density. However, as the seeding density increased, the CP, NDF, ADF, and TDN contents increased in both drill seeding and broadcast seeding. We found that, the dry matter content was the highest at the seeding density of 30 kg/ha for both the methods of seeding, but this was only 1.3 times higher than that of 10 kg/ha. Considering the seed price and labor force involved in seeding, it is advisable to have a seeding density of 15-20 kg/ha.

PURPOSES : This study analyzes the characteristics of nitrogen oxide concentration by applying titanium dioxide to existing roads in urban areas, using correlation analysis and a generalized linear model. METHODS : To analyze the characteristics of nitrogen oxide concentration with/without applying titanium dioxide to the urban road segment, data acquisition was conducted for nitrogen oxide concentration, weather information, and traffic information, etc., and a correlation analysis was conducted for each factor, with/without applying titanium dioxide to the roads. In addition, nitrogen oxide concentration generation models with/without the application of titanium dioxide to the roads were estimated using a generalized linear model. RESULTS : The results demonstrate that relative humidity and temperature were found to be slightly correlated with the nitrogen oxide concentration, both with and without the application of titanium dioxide to the roads; however, wind speed, solar radiation, and traffic volume were found to have somewhat low correlation according to the results of a correlation analysis. Moreover, relative humidity, temperature, solar radiation, and traffic volume were significant when titanium dioxide was applied to the roads, based on the estimated model from a generalized linear model, and the wind speed, solar radiation, and traffic volume were significant for the absence of titanium dioxide on the roads. CONCLUSIONS : Analytical results indicated that the characteristics of nitrogen oxide concentration vary depending on the application of titanium dioxide to the roads. In particular, when titanium dioxide was applied to the roads, the relative humidity and temperature were analyzed; according to both analyses, i.e., correlation analysis and a generalized linear model, the nitrogen oxide concentration was affected.