The study was performed to evaluate the effects of soybean meal (T1) and corn distillers dried grains with solubles (DDGS, T2) hydrolyzed by microbial proteolytic enzyme of Protame® on milk production and protein contents in dairy cows. Total of nine Holstein cows (i.e., 1.67 ± 0.47 average parity and 23.7 ± 1.2 kg/d average milk yield) were randomly assigned to control, T1, and T2 (e.g., 3 animals per group) and treated for 4 weeks. Milk yield of 3 different groups was similar in the beginning of the study, however the milk yield of T1 and T2 treatment group had increased by 0.93 kg/d and 1.89 kg/d, respectively. Milk protein level of T2 treatment group was increased by 0.19% (e.g., 0.14 kg/d protein content), whereas there was no significant different in control and T1 group. In conclusion, blood metabolites were ranged in normal level, but BUN content was reduced from 19.03 mg/dl to 17.70 mg/dl in T2 treatment group. This result suggests that DDGS hydrolysate supplement efficiently increase milk yield and milk protein level as well as feed protein availability in dairy cattle.

This experiment was conducted to determine the effect of increasing the dietary levels of nattokinase (NK) addi-tives on milk production and composition, and blood metabolites in dairy cows. NK additives with high fibrinolytic activity were produced by the strain with similarity to the Bacillus amyloliquefacines. A total 20 mid-lactating Holstein cows (average body weight 652.00 ± 4.38 kg; average 2.25 ± 0.35 parity; average milk yield 24.4 ± 0.38 kg/d) were randomly assigned to three treatments (5 animals per treatment). Cows were fed TMR supplemented with DDGS 100 g for control, NK additives 100 g and 200 g for T1 and T2 treatment, respectively for 4 weeks. By the increases of NK additives, milk yield increased for T1 (1.35 kg/d) and T2 (0.98 kg/d) at the final period than at the initial period (p < 0.05). But milk protein content decreased for T1 (3.20%) and T2 (3.24%) than for control (3.56%) (p < 0.05). Blood triglyceride, T-CHO and BUN levels in T1 decreased compared with that in control and T2 (p < 0.05). This finding showed that 100g of NK additives could possibly have a positive effect in lactation performance of mid-lactation Holstein cows by increasing milk yield, improving protein metabolism and decreasing cholesterol in blood.

본 연구의 목적은 양측성 단일 운동 훈련이 만성 뇌졸중 노인의 상지 운동기능회복에 어떠한 영향을 주는지 살펴보고 손상측 대뇌 운동피질 활성화에 미치는 영향에 대해 검증하고자 하였다. 이러한 연구 목적을 달성하기 위해 총 10명의 만성 뇌졸중 노인들이 양측성 훈련과 단측성 훈련에 각각 5명씩 참여하였으며, 실험을 위해 동작분석 시스템, 경두개자기자극기, 근전도 기기, 재활훈련도구가 사용되었다. 훈련과제는 나무 말뚝 옮기기, 나무 팩 맞추기, 그리고 물 마시기 모방 과제로 구성하여 주 2회 총 16회의 훈련을 실시하였다. 그 결과, 양측성 훈련 집단은 기능적 뻗기 검사에서 최대속도가 증가하였으며, 운동시간이 감소한 것으로 나타났다. 기능적 검사에서도 씨앗 옮기기, 무거운 물건 옯기기 과제에서 수행시간이 감소한 것으로 나타났다. 또한 경두개자기자극기를 활용한 신경생리적 검사에서도 130%, 140%, 그리고 150% 자극 강도에서 운동유발전위의 크기가 증가한 것으로 나타났다. 이러한 결과를 통해 양측성 단일 운동 훈련이 손상측 상지의 운동기능회복과 대뇌 운동피질 활성화에 긍정적인 효과가 있는 것으로 알 수 있다.