The faulty regulation of imprinting gene lead to the abnormal development of reconstructed embryo after nuclear transfer. However, the correlation between the imprinting status of donor cell and preimplantation stage of embryo development is not yet clear. In this study, to determine this correlation, we used the porcine spermatogonial stem cell (pSSC) and fetal fibroblast (pFF) as donor cells. As the results, the isolated cells with laminin matrix selection strongly expressed the GFRα-1 and PLZF genes of SSCs specific markers. The pSSCs were maintained to 12 passages and positive for the pluripotent marker including OCT4, SSEA1 and NANOG. The methylation analysis of H19 DMR of pSSCs revealed that the zinc finger protein binding sites CTCF3 of H19 DMRs displayed an androgenic imprinting pattern (92.7%). Also, to investigate the reprogramming potential of pSSCs as donor cell, we compared the development rate and methylation status of H19 gene between the reconstructed embryos from pFF and pSSC. This result showed no significant differences of the development rate between the pFFs (11.2±0.8%) and SSCs (13.3±1.1%). However, interestingly, while the CTCF3 methylation status of pFF-NT blastocyst was decreased (36.3%), and the CTCF3 methylation status of pSSC-NT blastocyst was maintained. Therefore, this result suggested that the genomic imprinting status of pSSCs is more effective than that of normal somatic cells for the normal development because the maintenance of imprinting pattern is very important in early embryo stage.

We present UBVRI CCD photometry of the Type Ie supernova SN 19941 in M51 which was discovered on April 2, 1994 (UT). UBVRI CCD photometry of SN 1994 I were obtained for the period of the first two months from April 4, 1994, using the Seoul National University Observatory 60 cm telescope. The light curves of SN 19941 show several interesting features: (a) SN 19941 reaches the maximum brightness at B-band on April 8.23 (B = 13.68 mag), at V-band on April 9.10 (V = 12.89 mag), and at I-band on April 10.32 (I = 12.48 mag); (b) The light curves around the maximum brightness are much narrower than those of other types of supernovae; (c) The light curves after the peak decline more steeply than those of other types of supernovae; and (d) The colors get redder from (V-R) ≈ 0.2 mag ((V - I) ≈ 0.3 mag, (B - V) ≈ 0.7 mag) on April 4 to (V-R)≈ 0.6 mag ((V-1) ≈0.9 mag, (B-V) ≈ 1.3 mag) on April 18. Afterwards (V - R) colors get bluer slightly (by ~0.005 mag/day), while (V-I) colors stay almost constant around (V-1) ≈ 1.0 mag. The color at the maximum brightness is (B-V)=0.9 mag, which is ~1 mag redder than the mean color of typical Type la supernovae at the maximum brightness. The light curves of SN 1994I are similar to those of the Type Ie supernova SN 1962L in NGC 1073. Adopting the distance modulus of (m-M)0 = 29.2 mag and the reddening of E(B - V) = 0.45 mag [Iwamoto et al. 1994, preprint for ApJ], we derive absolute magnitudes at the maximum brightness of SN 1994I, Mv(max) = -17.7 mag and MB(max) = -17.4 mag. This result shows that SN 1994I was ~2 mag fainter at the maximum brightness compared with typical Type Ia supernovae. A narrower peak and faster decline after the maximum in the light curve of SN 1994I compared with other types of supernovae indicate that the progenitor of SN 1994I might be a lower mass star compared with those of other types of supernovae.

Background : The advancement of next-generation sequencing technology dramatically reduces the cost for sequencing and it contributes to create a new research environment that utilizes large amount of genome sequences to answer many biological questions. With this new research trend, reference genome sequences of several major crops have been released to the research community and utilized in various researches in agriculture. Coupled with molecular breeding technology, NGS based genome research will possibly allow selecting a new plant material possessing useful traits in early stage and efficiently developing a superior cultivar. Methods and Results : The objectives of this research are to collecting various genetic variations (SNPs, indels and TE mediated variations) in major and minor crops, to develop molecular markers using NGS based genomic data (resequencing, GBS, transcriptome), and to develop a visualization tools to enhance the utility of the NGS data. Currently major analysis pipelines have been developed to detect SNPs, indel and polymophic SSRs using whole genome and transcriptome data, and a pipeline for identification of MITE insertion polymorphism is under development. In addition to that, for orphan crop, we also implemented an efficient and robust method to assemble a complete chloroplast, mitochondria and 45S rDNA using low coverage whole genome data in order to develop an inter- and intra-specific molecular barcode markers. Conclusion : NGS provide a new level of researches in many crop plants. Large amount of genomic information provides an opportunity to understand domestication and genetic variations, and to develop a better crop for future.

Drug-eluting stents (DES) have reduced the rate of restenosis and the need for repeated revascularization of target lesions. However, in stent restenosis is still a major issue and very late stent thrombosis seems to occur more frequently with DES. Recently, incidence of stent thrombosis as a fatal complication has increased and dual antiplatelet therapy is recommended for at least 12 months after implantation. Here we report on a case of ST-segment elevation myocardial infarction due to total occlusion caused by very late stent thrombosis and in stent restenosis simultaneously at 42 months after DES implanta-tion.

We report on the case of a 59-year-old man who presented with continuous chest discomfort. The patient's initial electrocardiogram (ECG) showed normal sinus rhythm. Coronary angiography showed significant stenosis in the proximal left anterior descending coronary artery (LAD), the distal left circumflex coronary artery (LCX). Therefore, we deployed a stent in the proximal LAD and the distal LCX. Two days after percutaneous coronary intervention (PCI), he complained of atypical chest pain. His ECG showed ST segment elevation in leads V1 to V3. In emergent coronary angiography, there was no stent thrombosis. ECG findings showed that the ST segment has a "saddle back" ST-T wave configuration in which the elevated ST segment descends toward the baseline, then rises again to an upright T wave, like the Brugada ECG pattern. This case report shows how dynamic ST segment elevations may look similar in cases of stent thrombosis after PCI.