The purpose of this study is to select various insect species for healing resources and develop a healing program in order to use insect as a healing agriculture. In this study, there are two kinds of breeding kit were developed, one for Gryllus bimaculatus and the other for Oxya chinensis sinuosa. Using these insect breeding kits, we conducted a survey of 60 children and the elderly. In the case of children, the results of the insect breeding satisfaction showed that 30.6% said that the sound of crickets was very good, and 11.1% said that it was good. In addition, the higher the child's awareness of insects, the higher the proportion of children who wanted to raise insects in the future. As a result of a survey of seniors, 45.2% do not like insects and 51.6% are not interested, meaning that most seniors are not very interested in insects. However, the emotions after breeding insects showed positive results, with 45.2% saying their personality became brighter, 48.4% reducing their anger, 48.4% relieving their irritation, 54.8% relieving loneliness, 58.1% feeling more responsible, and 51.6% developing intimacy.

갈색거저리 유충의 사료인 밀기울은 대부분 수입에 의존하고 있는데 일부 국가의 식량 수출 중단 조치 등에 따른 국제 곡물가격 상승으로 밀기울 가격은 인상되고, 식용곤충 판매가격이 하락하면서 생산비 절감을 위한 사료 개발이 요구되고 있다. 농업부산물 3종을 50% 이상 급이하게 되면 유충 생육이 저하되었기 때문에 본 연구는 적정한 배합비율을 선정하기 위해 첨가사료 20, 30% 함량으로 밀기울과 혼합하여 사료를 급이하였을 때 갈색거 저리 유충의 생육 특성과 영양성분 변화에 대해 밀기울만 제공한 대조구와 비교하였다. 부산물 A와 B, C를 각각 30% 함유한 처리구에서 갈색거저리 유충 무게는 대조구와 차이가 없음을 확인하였다. 먹이소화율은 부산물B를 20% 함유한 처리구가 80.5%로 대조구에 비해 높았고, 부산물C 30% 처리구에서 72.6%로 가장 낮았다. 갈색거저 리 유충의 생육일수 100일 기준으로 부산물B 30% 처리구에서 용화율이 76.1%로 대조구보다 1.6배 높았으며, 부산물A 20% 처리구는 29.2%로 용화율이 가장 낮았다. 갈색거저리 유충의 조단백질 함량은 부산물 C 30% 처리 구에서 대조구보다 10.3% 증가하여 아미노산 분석을 진행한 결과 sarcosine과 ornithine이 2.5배 이상 증가하였다. 이를 통해 부산물 B나 C를 30% 함유한 사료를 급이하였을 때 사육 원가를 절감하여 유충을 생산할 수 있을 것으로 사료된다.

Insect cuticular extracellular matrices (ECM) including the eggshell and exoskeleton play vital roles in protecting them from natural environmental stresses. However, these chitinous ECMs must be degraded at least in part during embryonic and post-embyonic molting periods to accommodate continuous growth all the way to the adult stage. In this study we investigated the functions of groups I and II chitinases, TcCHT5 and TcCHT10, in turnover of the eggshell and cuticle in Tribolium castaneum. RNAi and TEM analyses revealed that TcCHT10 is required for digestion of chitin in the serosal cuticle for embryo hatching as well as in the old cuticle during post-embryonic molts including larval-pupal and pupal-adult metamorphosis. However, although TcCHT5 is apparently involved in these vital physiological events, TcCHT10 could substitute for TcCHT5 except during the pupal-adult molting when both enzymes are indispensable to degrade chitin in the old pupal cuticle.

Aphis gossypii is a representative pest that transmits plant viral diseases. It is difficult to control with chemical pesticides alone due to their high pesticide resistance. Entomopathogenic fungi are biological control agents that can replace chemical pesticides and have characteristics of high host specificity and safety to humans. Therefore, we investigated the immune pathways of aphids against initial infection by entomopathogenic fungus. We treated aphids with the Beauveria bassiana JEF 544 strain and examined the immune response in early infection by qPCR. furthermore, we also studied changes the molting time of nymphs and changes in adult nymphal production caused by entomopathogenic fungi.

본 연구는 거리화단 화훼경관 조성 전·후의 스트레스 변화 를 알아보기 위하여, 순창 소재의 경찰관 총 18명을 대상으로 타액 채취 및 설문조사를 실시하였다. 생리 측정은 타액 코르 티솔, 혈압, 심리 측정은 식물 재배 활동 수준, 스트레스수준, 거리화단 화훼경관에 대한 간이 SD 감성을 측정하였다. 타액 코르티솔, 혈압의 사전/후 비교는 대응표본 t-test, 타액 코르 티솔, 혈압의 성별에 따른 집단간 사전/후 비교는, 독립표본 t-test, 스트레스수준, 간이 SD는 윌콕슨 부호 순위 검정 등으 로 분석하였다. 화훼경관 조성 전보다 조성 후 총 코르티솔 수 치가 낮아졌고, 식물 재배 활동 수준은 높아졌으나 통계적 유 의성은 없었다. 혈압은 여자의 경우 거리화단 화훼경관 조성 전과 조성 후 모두 정상 혈압이었고, 남자의 경우 거리화단 화 훼경관 조성 전 고혈압 전단계에서 조성 후 주의혈압으로 낮 아지는 긍정적 효과가 있었다. 거리화단 화훼경관 조성 전·후 의 스트레스수준을 총 4단계(총 4단계: 단계가 높을수록 스트 레스가 높은 수준임)로 문항 전체의 총합 수치로 알아본 결과, 화훼경관 조성 전에는 4단계(50.0%), 2단계(38.9%), 3단계 (11.1%) 순이었으나, 조성 후에는 4단계(44.5%), 2, 3단계(각 22.2%), 1단계(11.1%) 순으로 거리화단 화훼경관 조성 전에 는 1단계(정상적인 스트레스 상태로 스트레스요인 자체가 심 각하지 않거나 좋은 스트레스로 받아들인 경우)가 0%에서 조 성 후 11.1%로 높아졌다. 화훼경관 조성 전·후 SD법을 이용 하여 쾌적감, 자연감, 그리고 진정감에 대해 평가한 결과, 화 훼경관 조성 후 쾌적감, 자연감, 진정감이 높은 것으로 나타났 다(p<.05). 거리화단 화훼경관에 따른 식물 재배 활동 수준과 주요 변수간의 상관관계를 알아본 결과, 화훼경관 조성 후 식 물 재배 활동 수준과 스트레스수준, 스트레스수준과 진정감은 부(-)의 상관관계, 식물 재배 활동 수준과 쾌적감, 자연감, 진정 감과는 0.5 이상의 높은 정(+)의 상관관계가 있었다(p<.05). 따라서 식물 재배 활동 수준이 높아질수록 쾌적감, 자연감, 그 리고, 진정감은 높아지고, 거리화단 화훼경관이 없는 공간보 다 화훼경관이 있는 공간에서 쾌적감, 자연감, 그리고 진정감 을 더 크게 느끼는 것으로 판단되었다. 본 연구에서 거리화단 화훼경관을 통하여 고위험군의 높은 스트레스는 줄여주고 식 물에 대한 흥미, 관심과 심리적 안정효과는 높여 줄 수 있는 유용한 가능성이 있을 것으로 생각되었다.

Since rice is the main food in Korea, there are no regulations on corn milling yet. Corn is known as one of the world's top three food crops along with wheat and rice, and it is known that 3.5 billion people worldwide use corn for food. In addition, corn mills are not developed or sold in Korea, but the use of corn mills is increasing significantly in many countries in Southeast Asia. In the Philippines, as Korea's rice mill import increases, Korea's KAMICO (Korea Agricultural Machinery Industry Cooperative) and domestic company A agreed to develop a corn mill jointly with PHilMech, an organization affiliated with the Philippine Ministry of Agriculture. However, research on corn milling was very insignificant, so the development was carried out based on the technology of Korea's rice mill. Rice milling is performed by peeling off the skin of rice and producing brown or white rice, so it is carried out by removing the skin and cutting the skin. On the other hand, in the corn mill, the skin of the corn is peeled, pulverized and selected to produce main products suitable for edible use. Therefore, in order to develop a corn mill, processes such as peeling, transfer, grinding, sorting, and by-product separation are required, and suitable parts must be developed. In addition, the performance must be gradually improved through experiments in which corn is repeatedly milled. The Philippines produces 7.98 million tons/year of corn, which is about 100 times that of Korea, and is mostly consumed as a staple food. This is about 10% of the total crop production in the Philippines. In addition, the main cultivation complexes of corn are the mountainous regions of Tarlac or Pangasinan, and the produced corn is 72.4% of the so-called yellow corn called Arabel and Sarangani, and the remaining 27.6% are known as white corn. In this study, it was intended to produce grains of 2.5 mm or less suitable for food for yellow corn and to develop a corn mill for 200 kg per hour. Detailed conditions for development are stipulated as more than 55% of the main product recovery rate, more than 31% of the by-product recovery rate, less than 5% of the raw material loss rate, and more than 80% of the embryo dislocation rate. In this study, to achieve this, the overall process of the corn mill was developed, and the optimal conditions for the corn mill were obtained through the development of parts and empirical tests to improve performance. In addition, it was intended to achieve the development goal by evaluating and analyzing the performance of each part so that it did not conflict.

The initial development plans for the six reactor designs, soon after the release of Generation IV International Forum (GIF) TRM in 2002, were characterized by high ambition [1]. Specifically, the sodium-cooled fast reactor (SFR) and very-high temperature reactor (VHTR) gained significant attention and were expected to reach the validation stage by the 2020s, with commercial viability projected for the 2030s. However, these projections have been unrealized because of various factors. The development of reactor designs by the GIF was supposed to be influenced by events such as the 2008 global financial crisis, 2011 Fukushima accident [2, 3], discovery of extensive shale oil reserves in the United States, and overly ambitious technological targets. Consequently, the momentum for VHTR development reduced significantly. In this context, the aims of this study were to compare and analyze the development progress of the six Gen IV reactor designs over the past 20 years, based on the GIF roadmaps published in 2002 and 2014. The primary focus was to examine the prospects for the reactor designs in relation to spent nuclear fuel burning in conjunction with small modular reactor (SMR), including molten salt reactor (MSR), which is expected to have spent nuclear fuel management potential.

농촌진흥청 국립원예특작과학원에서는 2021년 분홍색계 소 형 호접란 ‘Arihong’을 육성하였다. 2014년 밝은 분홍색 소형 품종 Phalaenopsis ‘Wedding’와 백색 바탕에 분홍빛을 가지는 소형종 P. ‘[{KT1398-1×(KM-6)-4}×Chiangbeauty-88]-23’ 를 모본과 부본으로 교배하였다. 2018년 실생 120개체 중 잎자세, 화색, 화형, 꽃대수 등 특성이 우수한 ‘14104-1’ 개체를 선발하여 기내 화경배양을 통해 증식하였다. 2018년부터 2021년에까지 1차, 2차특성검정을 통해 품종의 안정성과 균일성을 확인한 후 ‘Arihong’로 명명하였다. 이 품종은 백색(WG155B) 바탕에 중앙 에는 보라빛 분홍색(PVG80B)을 띄며, 진한 자주색(PG78A) 순판 을 가지는 것이 특징이다. 꽃대가 2대씩 발생하고 꽃대 길이는 평균 42.1cm 소형 분화로 적절한 크기를 가지고 있다. 평피기 형태의 꽃은 길이와 폭이 각각 5.1, 5.7cm이며, 분지가 발생하여 1개의 꽃대에 13.0개의 소화가 착생한다. ‘Arihong’의 잎은 수평으로 자라며 길이와 폭이 각각 13.0cm, 4.9cm였다. 또한 초세가 우수하고 생육 속도가 빨라 엽수 확보가 용이한 특성을 보였다.

경상남도농업기술원 화훼연구소에서 2021년 화색이 연황 색이며 화심이 녹색인 미니 절화용 거베라 품종 ‘크림쿠키’ (Cream Cookie)를 육성하였다. ‘크림쿠키’ 품종은 2014년 황색 미니 ‘Sun City’를 모본으로, 백색 미니 ‘Blandy’를 부 본으로 인공교배를 실시하여 육성된 품종이다. 2021년까지 생육 및 개화 특성검정과 기호도 조사를 실시하였으며 ‘크림 쿠키’의 생육 및 개화특성을 대조품종인 ‘Sun City’와 비교하 였다. ‘크림쿠키’는 연황색(RHS 4C) 꽃잎과 녹색 화심을 가진 반겹꽃 거베라 품종이다. 화폭이 7.1cm인 작은 꽃이며, 화경 장은 58.9cm였다. 화경 직경은 상부 0.4cm, 하부 0.7cm 였 다. 외부설상화의 길이는 2.8cm이며 폭은 0.8cm였다. 개화 소요일수는 65.7일로 ‘Sun City’에 비하여 18.8일 빨랐으며, 첫 개화시 엽수는 22.4매였다. 연간 주당 절화수량은 102.3 본으로 ‘Sun City’의 82.0본에 비하여 20.3본이 많았다. 절 화수명은 17.8일로 ‘Sun City’보다 4.1일 더 길었다. ‘크림쿠 키’는 화폭이 7.1cm의 미니 품종으로 연간 주당 100본 이상 절화 생산이 가능하다. 또한 수확 후 꽃 캡 씌우기, 꽃목 보강 등 추가적인 작업을 생략할 수 있어 생산비 절감이 가능하므 로 농가보급 확대가 기대된다.

본 연구에서는 한국산 미기록속인 이마줄불청객파리속(신칭)과 미기록종인 작은눈이마줄불청객파리(신칭)를 처음으로 보고한다. 우리는 이 종이 포식성 거미의 섭식과정에 관여하는 phoretic relationship의 습성을 갖는 것을 확인하였다. 이 종의 성충에 대한 기재문과 사진 및 DNA바 코드 서열을 제공한다.

Foot-and-mouth disease (FMD), which affects cloven-hoofed animals, is economically important because of its highly contagious nature. FMD virus (FMDV), the causative agent of FMD, involves seven serotypes (O, A, Asia1, C, and SAT 1-3). Serotype Asia1 is unique to the Asian territory and is subdivided into nine genetic groups (G-I-IX) based on nucleotide variations in the VP1 sequence. Asia1 Shamir, the most representative Asia1 vaccine, is not highly protective against the Asia1/MOG/05 (G-V) lineage found in North Korea in 2007. Therefore, we investigated whether a chimeric virus strain (Asia1/MOG/Shamir), in which the VP4, VP2, and VP3 sequences of Asia1/MOG/05 were combined with the VP1 sequence of Asia1 Shamir, can simultaneously protect against both viruses. We determined the optimal viral growth conditions for the commercial utilization of this chimeric virus strain. Of the three types of cell culture media, the Cellvento medium resulted in the highest amount of antigen in the samples. The chimeric strain was proliferated in a small bioreactor to produce a test vaccine, and its immunogenicity was evaluated in pigs. The virus neutralization (VN) titer against the Asia1 Shamir virus was > 1/100 after the second immunization with the chimeric vaccine in pigs. In addition, a single dose of the test vaccine resulted in a VN titer of > 1/100 against the Asia1/MOG/05 strain. Taken together, our chimeric vaccine strain provided sufficient protection against the Asia1/MOG/05 and Asia1 Shamir viruses, suggesting its potential as a novel vaccine for both these strains.

In this study, we examined the antagonistic effects of sprout-borne lactic acid bacteria (LAB) on Salmonella enterica serovar Enteritidis. This antagonism is promoted as a means of controlling contamination during sprout production and provides additional LAB for consumers. We isolated a total of 24 LAB isolates in nine species and five genera from seven popular vegetable sprouts: alfalfa (Medicago sativa), clover (Trifolium pratense), broccoli (Brassica oleracea ssp. italica), vitamin (B. rapa ssp. narinosa), red radish (Raphanus sativus), red kohlrabi (B. oleracea var. gongylodes), and Kimchi cabbage (B. campestris var. pekinensis). Based on 16S rRNA gene sequences, the LAB species were identified as Enterococcus casseliflavus, E. faecium, E. gallinarum, E. mundtii, Lactococcus taiwanensis, Leuconostoc mesenteroides, Pediococcus pentosaceus, and Weissella cibaria, and W. confusa. A total of 16 LAB isolates in seven species including E. faecium, E. gallinarum, E. mundtii, L. taiwanensis, L. mesenteroides, P. pentosaceus, and W. cibaria showed antagonistic activity toward S. enterica. The growth inhibition of sprout LAB on S. enterica was confirmed by co-culture. Unexpectedly, sprout LAB failed to suppress the growth of S. enterica in alfalfa sprouts, whereas all LAB strains stimulate S. enterica growth even if it is not significant in some strains. The findings of this study indicate that S. enterica-antagonistic LAB are detrimental to food hygiene and will contribute to further LAB research and improved vegetable sprout production.

The domestic Pressurized Heavy Water Reactor (PWHR) nuclear power plant, Wolsong Unit 1, was permanently shut down on December 24, 2019. However, research on decommissioning has mainly focused on Pressurized Water Reactors (PWRs), with a notable absence of both domestic and international experience in the decommissioning of PHWRs. If proper business management such as radiation safety and waste is not performed, it can lead to increased business risks and costs in decommissioning. Therefore, the assessment of waste volume and cost, which provide fundamental data for the nuclear decommissioning process, is a crucial technical requirement before initiating the actual decommissioning of Wolsong Unit 1. Decommissioning radiation-contaminated structures and facilities presents significant challenges due to high radiation levels, making it difficult for workers to access these areas. Therefore, technology development should precede decommissioning process assessments and safety evaluations, facilitating the derivation of optimal decommissioning procedures and ensuring worker safety while enhancing the efficiency of decommissioning operations. In this study, we have developed a program to estimate decommissioning waste amounts for PHWRs, building upon prior research on PWR decommissioning projects while accounting for the specific design characteristics of PHWRs. To evaluate the amount of radioactive waste generated during decommissioning, we considered the characteristics of radioactive waste, disposal methods, packaging container specifications, and the criteria for the transfer of radioactive waste to disposal operators. Based on the derived algorithm, we conducted a detailed design and implemented the program. The proposed program is based on 3D modeling of the decommissioning components and the calculation of the Work Difficulty Factor (WDF), which is used to determine the time weighting factors for each task. Program users can select the cutting and packaging conditions for decommissioning components, estimate waste amount based on the chosen decommissioning method, and calculate costs using time weighting factors. It can be applied not only to PHWRs, but also to PWRs and non-nuclear fields, providing a flexible tool for optimizing decommissioning process.

증산은 적정 관수 관리에 중요한 역할을 하므로 수분 스트레스에 취약한 토마토와 같은 작물의 관개 수요에 대한 지식이 필요하다. 관수량을 결정하는 한 가지 방법은 증산량을 측정하는 것인데, 이는 환경이나 생육 수준의 영향을 받는다. 본 연구는 분단위 데이터를 통해 수학적 모델과 딥러닝 모델을 활용하여 토마토의 증발량을 추정하 고 적합한 모델을 찾는 것을 목표로 한다. 라이시미터 데이터는 1분 간격으로 배지무게 변화를 측정함으로써 증산 량을 직접 측정했다. 피어슨 상관관계는 관찰된 환경 변수가 작물 증산과 유의미한 상관관계가 있음을 보여주었다. 온실온도와 태양복사는 증산량과 양의 상관관계를 보인 반면, 상대습도는 음의 상관관계를 보였다. 다중 선형 회귀 (MLR), 다항 회귀 모델, 인공 신경망(ANN), Long short-term memory(LSTM), Gated Recurrent Unit(GRU) 모델을 구 축하고 정확도를 비교했다. 모든 모델은 테스트 데이터 세트에서 0.770－0.948 범위의 R2 값과 0.495mm/min－ 1.038mm/min의 RMSE로 증산을 잠재적으로 추정하였다. 딥러닝 모델은 수학적 모델보다 성능이 뛰어났다. GRU 는 0.948의 R2 및 0.495mm/min의 RMSE로 테스트 데이터에서 최고의 성능을 보여주었다. LSTM과 ANN은 R2 값이 각각 0.946과 0.944, RMSE가 각각 0.504m/min과 0.511로 그 뒤를 이었다. GRU 모델은 단기 예측에서 우수한 성능 을 보였고 LSTM은 장기 예측에서 우수한 성능을 보였지만 대규모 데이터 셋을 사용한 추가 검증이 필요하다. FAO56 Penman-Monteith(PM) 방정식과 비교하여 PM은 MLR 및 다항식 모델 2차 및 3차보다 RMSE가 0.598mm/min으로 낮지만 분단위 증산의 변동성을 포착하는 데 있어 모든 모델 중에서 가장 성능이 낮다. 따라서 본 연구 결과는 온실 내 토마토 증산을 단기적으로 추정하기 위해 GRU 및 LSTM 모델을 권장한다.

This study evaluated a potential sterilization process that uses calcium hypochlorite (Ca(ClO)2) as a disinfectant and hydrogen peroxide (H2O2) as a neutralizing agent for monoculture processes of microalgae (Nannochloropsis oculata). The results showed that no contaminants (prokaryote) were present when the Ca(ClO)2 concentration was greater than 0.010%. The use of an equivalent amount of H2O2 completely neutralized Ca(ClO)2 and had an additional bactericidal effect because of the formation of singlet oxygen. No substantial difference was observed in the biomass accumulation and chlorophyll contents compared to those in cultures sterilized using conventional physical methods such as autoclaving. Therefore, chemical sterilization using Ca(ClO)2 and H2O2 has an excellent economic advantage, and we expect the proposed ecofriendly chemical sterilization method to become a critical culture technology for microalgae-related industrialization.

PURPOSES : The evaluation of the low-temperature performance of an asphalt mixture is crucial for mitigating transverse thermal cracking and preventing traffic accidents on expressways. Engineers in pavement agencies must identify and verify the pavement sections that require urgent management. In early 2000, the research division of the Korea Expressway Corporation developed a three-dimensional (3D) pavement condition monitoring profiler vehicle (3DPM) and an advanced infographic (AIG) highway pavement management system computer program. Owing to these efforts, the management of the entire expressway network has become more precise, effective, and efficient. However, current 3DPM and AIG technologies focus only on the pavement surface and not on the entire pavement layer. Over the years, along with monitoring, further strengthening and verification of the feasibility of current 3DPM and AIG technologies by performing extensive mechanical tests and data analyses have been recommended. METHODS : First, the pavement section that required urgent care was selected using the 3DPM and AIG approaches. Second, asphalt mixture cores were acquired from the specified section, and a low-temperature fracture test, semi- circular bending (SCB) test, was performed. The mechanical parameters, energy-release rate, and fracture toughness were computed and compared. RESULTS : As expected, the asphalt mixture cores acquired from the specified pavement section ( poor condition – bad section) exhibited negative fracture performances compared to the control section (good section). CONCLUSIONS : The current 3DPM and AIG approaches in KEC can successfully evaluate and analyze selected pavement conditions. However, more extensive experimental studies and mathematical analyses are required to further strengthen and upgrade current pavement analysis approaches.