The large copper butterfly Lycaena dispar (Haworth, 1803; Lepidoptera: Lycaenidae) has been categorized as a near-threatened species (NT) in South Korea from 2012 mainly due to limited distribution. In this study, we visited 36 sites spread across all South Korean provinces to verify the distributional range of the species and sequenced mitochondrial COI for 53 individuals from nine sites. We observed L. dispar at 15 sites in six provinces, including the two previously known provinces, indicating a southward range expansion. The in-field monitoring and genetic data collectively suggested that L. dispar does not have a limited distribution nor is it isolated, indicating that it should be reclassified as less vulnerable. Our study demonstrates that the combination of field and genetic data can provide a more reliable assessment of the stability of a species.

The clearwing moth, Synanthedon bicingulata (Staudinger, 1887), is a pest that infests various species of cherry trees. However, genetic information regarding the genus Synanthedon including S. bicingulata, is limited. In this study, we sequenced a complete mitochondrial genome (mitogenome) of the species. The 16,255 bp of S. bicingulata mitogenome differs from the typical gene arrangement formed in Lepidoptera: trnQ-trnS2-trnM-trnI arrangement between the A+T-rich region and the ND2 junction. Moreover, the genome has untranslated repetitive sequences in the intergenic space between lrRNA and trnV, as well as the CGA start codon in COI and the TTG start codon in ATP8. Similar observations are noted in species belonging to the tribe Synanthedonini within the genus Synanthedon.

Aphis gossypii is a representative pest that transmits plant viral diseases. It is difficult to control with chemical pesticides alone due to their high pesticide resistance. Entomopathogenic fungi are biological control agents that can replace chemical pesticides and have characteristics of high host specificity and safety to humans. Therefore, we investigated the immune pathways of aphids against initial infection by entomopathogenic fungus. We treated aphids with the Beauveria bassiana JEF 544 strain and examined the immune response in early infection by qPCR. furthermore, we also studied changes the molting time of nymphs and changes in adult nymphal production caused by entomopathogenic fungi.

Tropilaelaps mercedesae Anderson and Morgan, 2007 (Acari: Laelapidae) is a serious ectoparasite of the brood of several honey bee species. Among the four recognized species of Tropilaelaps, Korean population was renamed as T. mercedesae from T. clareae on the basis of morphological evidences and genetic data. In this study, we report the complete mitochondrial genome (mitogenome) sequence of T. mercedesae. The 15,119-bp long mitogenome has an identical gene arrangement to that of Chinese sample reported previously. Comparison of two geographic samples showed COII, ND5, ND4, ND6, CytB, and ND1 to have higher number of variable sites than COI, which is often used for population-level study, suggesting these genes to have potential usefulness for population genetic study. The mitogenome sequence of T. mercedesae from Korea could be useful for species identification for geographic samples, trace of the origin of local populations, and illustration of evolutionary distinction among Tropilaelaps species either using part of or whole genome.

Vespa binghami (Hymenoptera: Vespidae) is one of the 10 species in the genus Vespa distributed in South Korea and is the only nocturnal wasp. In this study, we sequenced complete mitochondrial genome (mitogenome) of the species using the Sanger method and using Vespa-specific 30 primer sets. The length of V. binghami was 15,957 bp and the total A/T content was 80.6%. The A+T-rich region of V. binghami was 152 bp, and other Vespa species ranged from 39 bp (V. velutina) to 2,230 bp (V. v. auraria). Compared to the majority of insects, the gene arrangement of V. binghami had differences as followings: trnY-trnI-trnM-trnQ, trnN-trnE-trnS1-trnF, and trnS2-trnL1. However, all species in the genus Vespa registered in GenBank to date were composed of the same arrangement. Phylogenetic analysis using 13 PCGs and 2 rRNA genes showed the sister relationship between V. binghami and V. orientalis with the higher nodal supports.

Bombyx mandarina (Lepidoptera: Bombycidae), the presumed ancestor of the domesticated silkworm B. mori, has long been a subject of study to illustrate the geographic relationships in connection with origin of B. mori. We sequenced 100 mitochondrial genomes of B. mandarina collected from South Korea and Japan and these were combined with public data. Phylogenetic and population genetic analyses showed that all individuals collected in South Korea, except one individual from Inje, formed a strong group together with northern China and some individuals of southern China. This group was placed as the sister group to B. mori strains, suggesting that this group could have been served as an immediate progenitor for B. mori. For further robust inference more analysis is underway.

Spodoptera eridania and S. ornithogalli (Lepidoptera: Noctuidae), which are polyphagous pests that damage various crops such as tomatoes and beans are regulated quarantine species that are highly likely to invade South Korea. Therefore, it is crucial to promptly and accurately identify the presence of S. eridania and S. ornithogalli in crop fields to effectively eradicate as a regulated quarantine species. In this study, we developed a loop-mediated isothermal amplification (LAMP) assay, which allows for rapid in-field identification. To develop the LAMP　assay, we selected target species-specific genomic regions from the whole-genome sequences of one target and 13 other lepidopteran species. We validated each five and six primer sets that consistently produced positive reactions in S. eridania and S. ornithogalli, respectively. To test the sensitivity of the each locus, LAMP reactions were performed using various reaction times using crude DNA, which was extracted from various types of adult tissues. All sensitivity tests were also successful.

In the Republic of Korea, public health centers conduct vector mosquito control in accordance with infectious disease prevention laws. However, most public health centers have traditionally conducted periodic, uniform vector control across their respective regions without considering specific information on vector occurrence. In 2021, The Korea Diseases Control and Prevention Agency(KDCA) launched a control project to shift the paradigm toward mosquito control strategy based on mosquito surveillance data. In 2024, 18 local public health centers will participate in this project, which will progressively expanding so that it can be used countrywide. Local public health centers evaluate mosquito monitoring data using data gathered from Daily Mosquito Monitoring System(DMS), which enables them to pinpoint the best times and locations for vector control. Vector control activities carried out by local public health centers are computerized utilizing Vector Control Geographic Information System(VCGIS). Using the new control strategy with mosquito surveillance, the number of mosquito occurrences, number of control activities, and amount of insecticides have decreased compared to the periodic control activities. Based on mosquito surveillance data, it is anticipated that evidence-based mosquito vector control will offer a more efficient and effective means of mosquito control.

The complexation of silicon with carbon materials is considered an effective method for using silicon as an anode material for lithium-ion batteries. In the present study, carbon frameworks with a 3D porous structure were fabricated using metal–organic frameworks (MOFs), which have been drawing significant attention as a promising material in a wide range of applications. Subsequently, the fabricated carbon frameworks were subjected to CVD to obtain silicon-carbon complexes. These siliconcarbon complexes with a 3D porous structure exhibited excellent rate capability because they provided sufficient paths for Li-ion diffusion while facilitating contact with the electrolyte. In addition, unoccupied space within the silicon complex, combined with the stable structure of the carbon framework, allowed the volume expansion of silicon and the resultant stress to be more effectively accommodated, thereby reducing electrode expansion. The major findings of the present study demonstrate the applicability of MOF-based carbon frameworks as a material for silicon complex anodes.

The present study describes Philodromus paiki sp. nov., which was previously misidentified as P. fuscomarginatus (De Geer, 1778), P. poecilus (Thorell, 1872), and P. spinitarsis Simon, 1895 in Korea, as a new species with diagnosis, detailed descriptions, and taxonomic photographs. Additionally, P. spinitarsis is also described to correct previous misidentifications of Korean records of the species.

본 연구는 대한민국 울릉도 특산식물인 추산쑥부쟁이(Aster × chusanensis Y.S.Lim, Hyun, Y.D.Kim & H.C.Shin)의 기내 대량증식체계 확립을 위해 시토키닌 계열 식물생장조절 제를 첨가하여 캘러스 및 신초 형성 조건을 구명하고자 수행 하였다. 실험결과, 추산쑥부쟁이의 줄기 절편체에서 캘러스 와 신초가 형성되었다. 캘러스 형성률은 TDZ 3.0mg·L-1처 리구에서 88.9%로 가장 높았고 BAP 3.0mg·L-1와 Zeatin 3.0mg·L-1 처리구는 66.7%, 2iP 3.0mg·L-1 처리구에서는 11.1%였다. 신초 형성률은 모든 처리구에서 77% 이상이고 특히 TDZ처리구와 Zeatin 처리구에서 100%였다. 그러나 TDZ처리구에서는 형성된 신초가 부풀어 오르거나, 잎에 과수 성이 나타나는 등 형태적 기형이 나타났다. Zeatin처리구에 서 발생한 신초는 정상적인 형태를 나타났다. 잎 절편체에서 는 캘러스, 신초가 발생하지 않았으며 절편체가 탈색하며 고 사하는 것을 확인하였다. 종합적으로 추산쑥부쟁이의 캘러 스 및 신초 형성을 위해서는 줄기 절편체를 배양 재료로 이 용하는 것이 효과적으로 확인되었다. 캘러스 유도에는 TDZ 3.0mg·L-1처리가 가장 효과적이었고, 추후 NAA와 같은 옥신 을 함께 첨가하여 증식 효과를 분석하는 연구가 필요할 것이 다. 또한, 신초 유도에도 TDZ 3.0mg·L-1 처리가 효과적이었 으나 장기간, 고농도로 사용할 경우 형태적인 기형이 발생할 수 있어 정상적인 식물생산에 부적합한 것으로 확인되었다. 따라서 형태적 기형이 발생하지 않은 아데닌 유도체형 시토키 닌인 Zeatin을 활용하는 것을 추천한다. 본 연구는 울릉도 특 산식물인 추산쑥부쟁이의 기내 대량증식과 생태계 유지에 필 요한 종 공급을 위한 대량증식 체계 구축을 위한 자료로 활용 할 수 있을 것이다.

Truck no-show behavior has posed significant disruptions to the planning and execution of port operations. By delving into the key factors that contribute to truck appointment no-shows and proactively predicting such behavior, it becomes possible to make preemptive adjustments to port operation plans, thereby enhancing overall operational efficiency. Considering the data imbalance and the impact of accuracy for each decision tree on the performance of the random forest model, a model based on the Borderline Synthetic Minority Over-Sampling Technique and Weighted Random Forest (BSMOTE-WRF) is proposed to predict truck appointment no-shows and explore the relationship between truck appointment no-shows and factors such as weather conditions, appointment time slot, the number of truck appointments, and traffic conditions. In order to illustrate the effectiveness of the proposed model, the experiments were conducted with the available dataset from the Tianjin Port Second Container Terminal. It is demonstrated that the prediction accuracy of BSMOTE-WRF model is improved by 4%-5% compared with logistic regression, random forest, and support vector machines. Importance ranking of factors affecting truck no-show indicate that (1) The number of truck appointments during specific time slots have the highest impact on truck no-show behavior, and the congestion coefficient has the secondhighest impact on truck no-show behavior and its influence is also significant; (2) Compared to the number of truck appointments and congestion coefficient, the impact of severe weather on truck no-show behavior is relatively low, but it still has some influence; (3) Although the impact of appointment time slots is lower than other influencing factors, the influence of specific time slots on truck no-show behavior should not be overlooked. The BSMOTE-WRF model effectively analyzes the influencing factors and predicts truck no-show behavior in appointment-based systems.

This study explores the course tracking control problem of unmanned surface vessels (USVs) under the influence of actuator faults and internal and external uncertainties. In the control strategy desig n, we first model the unknown dynamics and use adaptive technology to construct an online appro ximator to compensate for the unknown dynamics of the system. Under the framework of adaptive backstepping, a robust adaptive course tracking control scheme is constructed. This control strategy does not require any prior knowledge of the model in advance. The stability analysis of the theoret ical mathematical derivation of the control strategy was conducted based on Lyapunov stability theo ry. Finally, the effectiveness of the control strategy proposed in this paper was verified through sim ulation.

As a treaty obligation and customary international law, universal jurisdiction is crucial in the global fight against impunity. While Western countries have been at the forefront, actively expanding and developing universal jurisdiction, East Asian countries have adopted a more conservative approach, emphasizing state sovereignty. This article begins by exploring the theoretical construction, legal frameworks, and state practices of East Asian countries (China, Korea, and Japan) when exercising universal jurisdiction, highlighting the typical differences between these countries and Western countries. It is then observed that the passive universal jurisdiction system in East Asia is deficient in defending state interests or counteracting other countries’ inappropriate extraterritorial jurisdiction. Accordingly, this article advocates a more proactive approach to the application of universal jurisdiction in East Asian countries. By doing so, while assuming responsibility to prevent impunity, the state can use universal jurisdiction as a legitimate countermeasure and reprisal in reciprocal international relations.

The radiological characterization of SSCs (Structure, Systems and Components) plays one of the most important role for the decommissioning of KORI Unit-1 during the preparation periods. Generally, a regulatory body and laws relating to the decommissioning focus on the separation and appropriate disposal or storage of radiological waste including ILW (intermediate level waste), LLW (low level waste), VLLW (very low level waste) and CW (clearance waste), aligned with their contamination characteristics. The result of the preliminary radiological characterization of KORI Unit-1 indicated that, apart from neutron activated the RV (reactor vessel), RVI (reactor vessel internals), and BS (biological shielding concrete), the majorities of contamination were sorted to be less than LLW. Radiological contamination can be evaluated into two methods. Due to the difficulties of directly measuring contamination on the interior surfaces of the pipe, called CRUD, the assessment was implemented by modeling method, that is measuring contamination on the exterior surfaces of the pipes and calculating relative factors such as thickness and size. This indirect method may be affected by the surrounding radiation distribution, and only a few gamma nuclides can be measured. Therefore, it has limitation in terms of providing detailed nuclide information. Especially, α and β nuclides can only be estimated roughly by scaling factors, comparing their relative ratios with the existing gamma results. To overcome the limitation of indirect measurement, a destructive sampling method has been employed to assess the contamination of the systems and component. Samples are physically taken some parts of the systems or components and subsequently analyzed in the laboratory to evaluate detailed nuclides and total contamination. For the characterization of KORI Unit-1, we conducted the radiation measurement on the exterior surfaces of components using portable instruments (Eberline E-600 SPA3, Thermo G20-10, Thermo G10, Thermo FH40TG) at BR (boron recycle system) and SP (containment spray system) in primary system. Based on these results, the ProUCL program was employed to determine the destructive sample collection quantities based on statistical approach. The total of 5 and 8 destructive sample quantities were decided by program and successfully collected from the BR and SP systems, respectively. Samples were moved to laboratory and analyzed for the detail nuclide characteristics. The outcomes of this study are expected to serve as valuable information for estimating the types and quantities of radiological waste generated by decommissioning of KORI Unit-1.

The periodic safety review (PSR), for all operating nuclear power plants in Korea, has been conducted in accordance with SSG-25, a guideline suggested by the IAEA, The PSR is performed through the review of the regulatory body after the operator’s self-evaluation. In order to guarantee a high level of safety in consideration of the changed environment, such as operating experience (OE) and technology development, it should be comprehensively and integratedly performed, and it is also carried out every 10 years after the operation permit. However, in case that all or part of the reactor facilities have been permanently shut down, such as Kori Unit 1 and Wolsong Unit 1, Around a half of reactor facilities are not in operation. The periodic safety evaluation may not be conducted for unused parts if there is no safety hazard and if there are some difficulties for applying periodic safety evaluation. In considering that the biggest purpose of PSR safety (by PSR definition of KINS guideline) is to improve and accumulated factors such as aging deterioration, facility change, operation experience, and technological development for operating nuclear power plants. It refers to a comprehensive safety evaluation that is periodically performed during the period of operation of a nuclear power plant. It is necessary to review whether PSR should be performed for a nuclear power plant that is permanently shut down after nuclear power plant operation is terminated. Also, in IAEA SSR 2/2 Rev1, it is defined that PSR is performed during the nuclear power plant operation period. “Requirement 12: Periodic safety review, Systematic safety assessments of the plant, in accordance with the regulatory requirements, shall be performed by the operating organization throughout the plant’s operating lifetime, with due account taken of operating experience and significant new safety related information from all relevant sources”. Recently, Kori Unit 1 and Wolsong Unit 1 were decided to permanently shut down in June 2017 and December 2019, and are currently being prepared for decommissioning. According to the Wolsong decommissioning plan, decontamination and demolition will be completed by 2032. The PSR for permanent shutdown of Kori Unit 1 was submitted to the regulatory body in December 2018 and is under approval review. In the case of the permanent shutdown PSR of Wolsong Unit 1, the project will be launched in May 2023 and the PSR will be submitted to the regulatory body in May 2024. In the case of Wolsong Unit 1, it is necessary to operate the various systems, including the systems related to the spent fuel storage tank, even during the period of permanent shutdown. Such as the heavy water related systems used in common with Wolsong Unit 2, are essential operating systems. Based on Basic Subject Index (BSI), 112 out of 218 systems require operation, indicating that about 50% of systems require operation even after permanent shutdown. Decommissioning of systems and equipment will begin after the transfer to modular air-cooled canister storage (MACSTOR) by the end of 2025, and then in-depth discussions will be needed whether PSR evaluation is meaningful.

In order to evaluate the integrity of the reactor pressure vessel, various test specimens necessary to identify irradiation embrittlement. The degree of irradiation embrittlement of the vessel material by neutrons, from the construction to the end of the life of the plant, is evaluated by a monitoring plan that called surveillance program (a series of all plans to analyze and evaluate embrittlement through various tests and analyzes by placing a test piece inside the reactor pressure vessel and taking out a piece at an appropriate time according to the number of operation years and taking necessary measures for safe operation). The reactor monitoring specimens for Kori Unit-1 are located by axis at S (57°), T (67°), R (77°), N (237°), P (247°) and V (257°). Six surveillance capsules are attached to the inside of the pressure vessel around the core and to the outside of the thermal shield. This surveillance container determines the withdrawal timing of the surveillance container according to the provisions of ASTM E185-82. In the monitoring test piece, there are neutron dosimeter materials to measure and evaluate the irradiated neutron flux, and Ni, Cu, Fe, Co-Al, Cd, and shielded Co-Al monitors are wired in the monitoring container. Each axial position is contained in a spacer hole. The neutron dosimetry monitor measures the neutron dose using isotopes produced by neutrons during operation of the reactor. The Al-Co specimen, which can evaluate the degree of radioactivity of cobalt, is located on the lower part of the specimen. The content of Co in the Al-Co specimen is 0.15%, and when expressed in ppm, it is 1,500 ppm, which is similar to the cobalt content of 1,414 ppm in the internal structure of the reactor vessel presented in NUREG-3474. If the radiation value of the Al-Co sample in the reactor monitoring specimen can be measured, the radiation value of the internal structure of the reactor can be indirectly compared. Since the monitoring specimen is located outside of the thermal shield, radiation should be less than that of the thermal shield. Korea Reactor Monitoring Technology performed gamma measurement on Al-Co specimens in 6 monitoring specimens, and although there are differences depending on the sample, it shows radioactivity values around the order of 1E+07 dps/g, or Bq/g. In conclusion, it is thought that using this measurement values, it is possible to verify the evaluation of internal structure radiation for Kori unit-1 decommissioning.

Over the past decades, particle physics has made significant progress in characterizing neutrinos even if neutrinos have extremely small cross-section (~10-44 cm2), allowing them to penetrate any object. More recently, neutrino detection and analysis have indeed become valuable tools in various aspects of nuclear science and technology. Neutrinos are detected using various methods, including Inverse Beta Decay (IBD), Neutrino-electron scattering, and Coherent Neutrino-Nucleus Scattering (CNNS). For the detection of anti-neutrinos from nuclear reactor, the Inverse Beta Decay (IBD) is commonly considered with scintillators. Notable experiments in Korea, such as RENO and NEOS, have been conducted using the IBD method at the Hanbit Nuclear Power Plant since 2006. Additionally, the NEON experiment, which employs CNNS, which has a significantly larger reaction cross-section than IBD but its low-energy signal detection difficulty, has been ongoing since 2021. Based on the results of NEOS (2015-2020) the signal to noise is ~30 and IBD detection rate is ~2000 counts per day. The IBD event in nuclear power plants provides valuable information about reactor behavior. IBD count rates are in good agreement with the thermal power of the reactor. Furthermore, the neutrino energy spectrum can be used to estimate the fission isotope ratio of the reactor core, showing promise for obtaining reactor core information from antineutrino detection techniques. Neutrino detection in nuclear facilities provides valuable information about reactor behavior. However, as a surveillance technology neutrino detection faces challenges due to the very low cross-section, requiring efforts to overcome limitations related to detector size and signal acquisition time. In 2008, the International Atomic Energy Agency (IAEA) included neutrino detection in its Research and Development (R&D) program for reactor safeguards. In January 2023, the IAEA organized a “Technical Meeting on Nuclear Data Needs for Antineutrino Spectra Applications” to discuss the latest developments and research results in this field. In summary, the use of neutrino detection in the nuclear field, particularly for reactor monitoring and safeguarding, has advanced significantly. Ongoing research and collaboration are expected to enhance our understanding of neutrinos and their applications in nuclear science and technology.