경남의 벼농사 대표 지대인 남부 중간지와 남부 평야지에서 질소 감비(7 kg/10 a)에 의한 고품질 쌀 생산을 위한 품종을 선발하고자 본 연구가 수행되었다. 7품종의 벼를 중간지에서 재배하였고 평야지에서는 10품종의 벼를 재배하여 평가하였다. 중간지에서 완전립의 수량은 보라미(526 kg/10 a)를 제외하고 대부분의 품종이 대조품종인 추청벼(533 kg/10 a)에 비해 높게 나타났다. 반면, 보라미와 고품벼, 온누리는 완전립률이 다른 품종에 비해 낮게 나타났다. 더욱이, 보라미와 대조품종인 추청벼는 도복지수가 다른 품종에 비해 높게 나타났다. 도정된 대조품종인 추청벼의 단백질 함량은 6.1%로 나타났고, 다른 품종들은 대조품종에 비해 비슷하거나 낮게 나타났다. 평야지에서는 남평벼(527 kg/10 a)만이 완전립 수량에서 대조품종인 일미벼(503 kg/10 a)에 비해 높게 나타났다. 그리고 호품벼, 신동진 그리고 칠보벼는 다른 품종들에 비해 완전립 비율이 더 낮게 나타났다. 그러나 호품벼(5.9%)와 진수미(6.0%), 신동진(5.9%), 칠보벼(6.0%), 황금노들(5.8%), 호평벼(6.0%)는 대조품종 일미벼(6.1%)에 비해 단백질 함량이 더 낮게 나타났다. 아밀로오스 함량, 백도, 식미 등은 두 지역에서 품종들 간 유의적 차이는 없었다. 결론적으로 완전립 비율, 완전립 수량, 단백질 함량, 도복 저항성, 등숙률 그리고 식미 등을 고려하면 중간지에서는 풍미1호, 새누리, 칠보벼가 평야지에서는 진수미, 황금노들이 고품질의 쌀을 생산하는데 적합한 품종으로 판단된다.

To breed a new Korean Pleurotus eryngii cultivar with high quality and yield, single crosses between 24×46 and KNR2539 were performed, and a new cultivar, 6×13, was selected based on the days to harvest (14.6 days), quality (7.2), and yield (85.9 g/850 cc bottle). The strain was named Aeryni 5 and cultivated on a large scale at the mushroom farms to compare with Keuneutari 2ho. The yield of Aeryni 5 (82.2 g) was 122.7% of Keuneutari 2ho, and the quality of the new cultivar was 7.7 while reference cultivar was 6.3. The yield and quality of the two cultivars were statistically different. The lightness of the pileus of Aeryni 5 (61.7) was greater than that of Keuneutari 2ho by 3.4 points; thus, the pileus of Aeryni 5 looked brighter. PCR with URP2 was used to discriminate between Aeryni 5 and Kenneutari 2ho.

Pleurotus eryngii, an edible white-rot fungus, is widespread in Eurasia and northern Africa. It has become a major cultivated mushroom in Asia, with a current global production rate of approximately 3 × 10 5 metrictons/yr. To improve the quality or productivity through breeding, a genetic linkage map is an important component. In this study, genetic linkage map of the P. eryngii was constructed using 98 monokaryotic progeny derived from dikaryon of parental KNR2312 strain derived from haploid meiotic spores. The whole genome sequence of P5 monokaryon from P. eryngii KNR2312 strain by Next Generation Sequencing (NGS) strategy was used to design the SSR markers. 484 primers pairs were identified by SSR Locator I and tested polymorphism via PCR. A total of 241 loci were mapped using Joinmap 4.0, comprising 222 SSR markers, 2 mating type factors, and the 13 INDEL markers. The map consisted of 14 linkage groups spanning 1003 cM at an average marker interval of 4.2 cM. The mating loci, A and B were mapped on linkage groups 4 and 11, respectively. The established linkage map and the genetic information based on NGS could be used for QTL mapping of agronomic traits, marker-assisted breeding that may ultimately lead to outstanding phenotypic characteristics. [Supported by a grant from the IPET (213003-04-3-SBY20), MIFAFF, Republic of Korea.]

Differentiation of Pleurotus eryngii is laborious and time-consuming tasks especially in mycelial status. For development of a method for differentiation of P. eryngii cultivars, simple sequence repeats (SSR) from whole genomic DNA sequence analysis was used for genotyping and two multiplex-SSR primer sets were developed. These SSR primer sets were employed to distinguish 12 cultivars and strains. Five polymorphic markers were selected based on the genotypes. PCR with the each primer produced one to four distinct bands ranging in size from 200 to 300 bp. Polymorphism information content (PIC) values of the five markers were in range of 0.6627 to 0.6848 with an average of 0.6775. Unweighted pair-group method with arithmetic mean clustering analysis based on genetic distances using five SSR markers classified 12 cultivars into 2 clusters. Cluster I and II comprised of 4 and 8 cultivars, respectively. Two multiplex sets, Multi-1 (SSR312 and SSR366) and Multi-2 (SSR178 and SSR277) completely discriminated 12 cultivar and strains with 21 allele with a PIC value of 0.9090. These results might be useful to provide an efficient method for the identification of P. eryngii cultivars with separate PCR reactions. (This work was supported by a grant from the Gold Seed Project [Supported by a grant from the IPET (213003-04-3-WTI11), MIFAFF, Republic of Korea.]

A new commercial cultivar ‘Hae Sal’ of Hypsizigus marmoreus was developed by the method of mono-mono crossing between monokaryotic strains derived from KNR8047 and KNR8080. The parental strains are characterized by the property of high quality and a short period of time in cultivation respectively. The optimum temperature of mycelial growth was 25°C and that of fruiting body development was 15～16°C. The period of harvesting including primordia formation was 7.0 days shorter than that of control strain ‘Mangadak No.2’. The color of pileus and stipe surface was neutral-brown and pure white, respectively. The yield was 117.6±23.2g/850cc plastic bottle. Analysis of the genetic characteristics of the new commercial cultivar ‘Hae Sal’ showed a different profile as that of the control strain, ‘Mangadak No.2’, when RAPD (Random Amplified Polymorphic DNA) primer was used. This new cultivar ‘Hae Sal’ of Hypsizigus marmoreus is characterized by the improved quality and a short period of time in cultivation after scratching. It would be of much help to expand the cultivation area of Hypsizigus marmoreus.

향후 쌀 생산량 조정과 조사료 자급률 향상을 위해 사료용 벼 재배면적이 확대될 시기에 국내 총체사료용 벼 종자생산체계 확립에 기술적 기초자료를 확보하기 위해 총체사료용 벼 주요품종의 채종적지 선정에 관해 검토한 결과는 아래와 같다. 1. 벼 재배 지대별 10개소 중 ‘녹양’은 I-2지대 남부해안지인 고성, II-1지대 호남평야지인 익산, II-2지대 영남평야지인 밀양 지역이 충실한 종자를 채종하기에 적합한 지역으로 조사되었다. 그 중에서도 고성지역에서 4월 30일에 파종하여 5월 30일에 이앙했을 때 생육, 수량 및 발아특성이 가장 좋아 채종적지로 가장 적합할 것으로 판단된다. 2. ‘목우’는 I-2지대 남부해안지인 고성, II-1지대 호남평야지인 익산지역이 우량 종자를 채종하기에 적합한 지역으로 보인다. 특히 남부해안지인 고성에서 파종시기를 앞당겨 4월 1일에 파종하여 4월 30일에 이앙했을 때 가장 건전한 종자를 채종할 수 있을 것으로 판단된다. 3. ‘목양’은 지대별로 공시한 다른 3품종과 비교했을 때 등 숙비율, 발아율 등이 대체로 낮아 다른 3품종보다는 채종적지로서의 조건이 다소 미흡하지만 I-2지대 남부해안지인 고성, II-1지대 호남평야지인 나주지역이 채종하기에 적합한 것으로 나타났다. 남부해안지인 고성에서는 ‘목우’와 마찬가지로 4월 1일 에 파종하여 4월 30일에 이앙했을 때 가장 건전한 종자를 얻을 수 있으나 평균발아율이 85%미만으로 건전한 발아를 위해서는 채종한 종자를 최소한 반드시 수선하여 사용해야 하며 수선 시에는 채종량에 비해 종자량의 손실이 많을 것으로 판단된다. 4. ‘중모1029’의 경우에는 지대별로 시험한 10개소 중에서 II-2지대 영남평야지에 속하는 대구지역이 생육, 수량 및 발아 특성을 고려해 볼 때 채종적지로 판단된다.

Chinese milkvetch(CM) has been cultivated as green manure source for environment friendly farming in southern area. This experiment was conducted to produce good quality rice through studying the changes of yield, quality and leaf colors as affected by the amount of CM biomass retuned to paddy field. We growed rice, transplanted in June 10 with cultivar dongjinilho, in four different plots retuned green manure with 0, 1.5, 3.0, and 4.5 ㎏ m-2 CM biomass in May 30. Nitrogen amounts were 5.5g by 1.5㎏CM biomass, 11.1g by 3.0㎏, and 16.6g by 4.5㎏ in late May. There were no different SPAD values among treatments in 10 days after transplanting. However, the more the CM biomass from 20 days after transplanting, the higher the SPAD values were. the plot of CM biomass to 3.0 ㎏ m-2 had same heading date but the plot of 4.5 ㎏ m-2 was one day late heading date as compared with the others. There were no different plant height, number of panicle, panicle length, number of leaf and ripening ratio among the CM biomass amounts. The higher the CM biomass amount, the more likely the plant was lodge. the plot retuned CM fresh biomass 1.5 ㎏ m-2 showed the higher rice yield than the plot retuned 3.0 and 4.5 ㎏ m-2 in 2006, but all plots had similar rice yields in 2007. There was no different whiteness of rice among all treatments . The more the CM biomass amount retuned to plot, the higher the protein content of rice.