This study aims to evaluate the anti-inflammatory effect of Auricularia auricula-judae ethanol extract (AEE) in LPS-induced RAW264.7 cells and dextran sulfate sodium (DSS)-induced acute colitis mice. The highest levels of total polyphenols and DPPH radical scavenging activity were observed in AEE. Also, NO production was reduced in a dose-dependent manner in RAW264.7 cells stimulated by lipopolysaccharide (LPS). AEE was suspended in distilled water and administration per oral at different doses of 100 and 300 mg/kg body weight every day with 3% DSS in drinking water for 7 days. The sample AEE 100 and 300 mg/kg significantly increased body weight, colon length and decreased disease activity index (DAI) score. Histological features showed that 100 and 300 mg/kg of AEE suppressed edema, mucosal damage and the loss of crypts induced by DSS. The serum levels of TNF- and IL-6 were measured in acute colitis mice using ELISA kits. Levels of TNF- and IL-6 in serum were significantly decreased by topical application of AEE. Therefore, AEE increases antioxidant and anti-inflammatory activity, and it is thought that it can effectively help prevent colitis.

Cats form social groups in which individuals recognize each other, and the cohesiveness of the group is maintained by a variety of amicable behaviors. We are aimed to provide the available knowledges about the prevention and correction methods for the behavior problems in cats through literature review. As the results, The behavior problems of cats may occur between group members and between group members and non-group members. Within the domestic environment, the behavior problems of cats may become a problem when it is directed at house-mates or humans. Differential diagnosis and treatment of various problems of the behavior problems of cats are discussed.

Companion animal is an animal kept primarily for a person's company or protection. The most popular pcompanion animals are noted for their attractive appearances and their loyal or playful personalities. Companion animals commonly provide their owners physical and emotional benefits. Companion animals can give companionship to elderly adults who do not have adequate social interaction with other people. We are aimed to conduct the survey research to evaluate the perception of twenties's interviewers on the companion animals. We deals with the findings obtained from extensive interviews with a representative samples. The objectives of interviews included twenties year-old ages. Questions focused on how interviewers were recognized the perception of companion animals. Questionnaire and technical data are analysed and summarized. As the results of the survey research, the objectives of interviews had positively understood about the perception of companion animals.

Mycoplasma (M.) hyopneumoniae is the causative agent of swine enzootic pneumonia, a disease that is prevalent in every country where pigs are raised. In this study, we aimed to develop a sensitive and specific PCR assay to detect M. hyopneumoniae in pigs. The suitability of this PCR assay for the detection of mycoplasmal infection was also tested using clinical lung samples from slaughtered pigs. We de- veloped a probe and M. hyopneumoniae-specific primer pairs, MhyoP-F and MhyoP-R, for the new PCR assay based on regions in the Mycoplasma protein P97 gene that are unique to M. hyopneumoniae. The developed PCR as- say was very specific and sensitive for the detection of M. hyopneumoniae. The assay was able to detect the equivalent of 10 pg of target template DNA, which indicates that the assay was very sensitive. In addition, the M. hyopneumoniae PCR assay detected only M. hyopneumoniae and no other Mycoplasma spp. or bacterial species of another genera. Further, the newly developed PCR assay effectively detected M. hyopneumoniae infection in pigs. We suggest that this PCR assay using M. hyopneumoniae-specific primer pairs, MhyoP-F and MhyoP-R, will be useful and effective for monitoring M. hyopneumoniae infection in pigs.