본 연구는 버섯재배부산물, 재활용가금깔개, 미강 및 straw로 구성되는 BF 사일리지 급여가 육성 거세한우의 행 동양상에 미치는 영향을 규명하기 위하여 실시하였다. 시 험에 사용한 공시 한우는 11개월령 총 10두(평균 301.7 kg) 를 2처리로 배치하였으며, 이 때 대조구(볏짚 자유채식)와 처리구(BF silage 자유채식)로 하여 48시간 행동관찰을 실 시하였다. 대조구와 비교해서 BF 사일리지 급여구는 총 DM 섭취량과 NDF 섭취량이 각각 30% 및 36% 높았고, 채식시간, 반추시간 및 저작시간은 유의적인 차이가 없었 다. 식괴수, 식괴당 반추시간에 있어서도 유의적 차이가 없 었고, 식괴당 저작수와 FVI는 낮게 나타났다(p<0.05). 또한 대조구와 비교해서 BF 사일리지 급여구는 배뇨 회수가 높 고(p<0.05), 음수 및 배분 회수에 있어서는 상호간에 차이 가 없었고, 채식율, 반추효율 및 저작효율에 있어서는 더 높게 나타났다(p<0.05). 이상의 결과로 볼 때, BF 사일리지 급여는 볏짚과 비교 시 육성거세한우의 반추행동 상 큰 차 이가 없다는 결과로 미루어 볼 때 차후 볏짚 대용으로 활 용하여도 좋을 것으로 사료되었다. 즉 입자도가 큰 straw를 peNDF를 충족시키는 최소량을 혼합하여 부산물사료 사일 리지를 제조, 급여하였을 때 육성거세한우의 반추행동은 볏짚 급여 시와 차이가 없었다.

The cotton aphid, Aphis gossypii Glover is an important sap-sucking pest of many pant, including cucumber and pepper. The objective of the present study was to determine the effects of sublethal concentrations of two insecticides (imidacloprid and flonicamid) and the action mechanisms on the feeding behavior of A. gossypii. The median lethal concentrations (LC50) of imidacloprid and flonicamid for adult A. gossypii were 2.01 and 1.92 ppm, respectively. The sublethal concentrations of imidacloprid were 0.22 ppm (LC10) and 0.82 ppm (LC30), and those of flonicamid were 0.094 ppm (LC10) and 0.56 ppm (LC30). The developmental period of A. gossypii nymphs at LC30 was 3.6 days for both insecticide which shorter than controls (4.2 days). Adult longevities at LC10 and LC30 of imidacloprid were 15.2 and 13.6 days, respectively. Adult longevity at LC10 and LC30 of flonicamid was 11.1 and 9.9 days, respectively. Control adult longevity was 15.5 days. Total fecundity was decreased at both sublethal concentration of two insecticides. Feeding behavior analysis using an electrical penetration graph showed that sublethal doses of imidacloprid and flonicamid had significant effects on the duration of phloem ingestion. However, higher doses of flonicamid induced starvation by inhibition of phloem ingestion and higher doses of imidacloprid induced contact toxicity rather than inhibition of feeding behavior.

 ,  , To investigate host preferencse of Myzus persicae on 15 sweet pepper cultivars grown in Jeonnam Province, EPG (electrical penetration graph) and life table experiments were carried out in the laboratory. Phloem phase times were significantly longer on Ferrari, Jinju, Debla, and Rapido than Orobell and Thialf. Non-penetration times were conversely observed. Life span, reproduction period, total fecundity, and intrinsic rate of increase (r<, SUB>, m<, /SUB>, ) of M. persicae, were higher on Ferrari, Debla, Orange glory, and Jinju than on Purple, GreenAce, Orobell, and Thialf. On the bases of these results, we conclude that M. persicae preferred Ferrari, Jinju, Orange glory, and Debla among the 15 tested sweet pepper varieties. However, we could not show the preference of the aphid for Purple, Orobell, and Thialf.

Aphids feed on host plants by penetrating the stems or leaves with stylets. The feeding behavior of aphids consists of probing, penetration, salivation, and sap ingestion. To assess the effects of sound on feeding behavior, we monitored the stylet activity of the green peach aphid, Myzus persicae (Sulzer), using electrical penetration graph (EPG). The use of EPG was critical for determining the stage, frequency, and duration of feeding in aphids. We played back four acoustic stimuli of sine waves with frequencies of 100, 500, 1000, and 5000 Hz to adult aphids. When the sound was treated, the frequencies of probing, penetration, and salivation increased, whereas the duration of sap ingestion decreased. The 100 Hz and 500 Hz was significantly effective to disturb ingestion of phloem sap. The results of EPG revealed that the acoustic stimuli may restrict aphid feeding by disturbing sap ingestion.

This study was performed to investigate the effect of flonicamid and thiamethoxam treated at sublethal concentration (LC10, LC30) on development period, adult longevity and fecundity and the feeding behaviour of Myzus persicae adult. Developmental period of M. Persicae nymph took 5.9 days in LC10, and 6.1 days in LC30 in both insecticides, comparing with control (5.7 days), it showed longer than those of the control, but there was no significance. Adult longevity treated at LC10 and LC30 of flonicamid was showed 13.2 and 13.7 days, respectively, and LC10 of thiamethoxam was examined as 14.7 days, it showed longer than control of 11.6 days. Mean daily fecundity exhibited higher in LC10 (3.1) and LC30 (3.1) of flonicamid than that of control (2.5), but thiamethoxam are not. Total fecundity exhibited higher in LC10 (41.8) and LC30 (43.0) of flonicamid, in LC10 (42.1) of thiamethoxam than that of control (29.5). Feeding behavior was examined using EPG (electrical penetration graph). EPG data indicated that flonicamid and thiamethoxam increased the duration of non-probing periods and decreased the duration of phloem ingestion.

Pyrifluquinazon, as a quinazinalone chemical group, based on a new mode of biological activity. It is reported that mode of action is modifies insect behavior, rapidly stopping feeding such that insects starve to death. Time-release feature and mortality effect on M. persicae using different pyrifluquinazon nano type and non-nano type were compared. Pyrifluquinazon nano type was formulated with different molecular weight and density of used chitosan (CS 30000 0.1% and CS 3000 0.3%). In the CS 30,000 0.1%, the mortality was weakly occurred at early time, but steadily increased after 4days. Finally, we confirmed more than 70% mortality as a peak at 16days. In CS 3000 0.3%, the mortality showed about 70% until 18days as a effective controlled release. Also, We examine time-release feature and mortality effect on M. persicae according to the different pyrifluquinazon nano type(CS 30000 0.1% and CS 3000 0.3%) of concentrations. The CS 30000 0.1% bioassay results of different concentration were showed that the highest concentration(100ppm) was measured better mortality than other concentration at 0 day, but cannot confirm different effect about dissimilar concentration. However, increasing rates of M. persicae were low as treatment concentrate was high. In CS 3000 0.3% 100ppm concentration bioassay result, aphid mortality reached peak at 24 days and increasing rate also low. Additionally, for the comparing of bioassay and feeding behavior of M. persicae against pyrifluquinazon nano types and non-nano type, EPG technique was carried out. In case of non nano type, feeding inhibition efficacy was showed during 4 days after treatment, but appeared similar level with control after 10days. In CS 3000 0.3% 50ppm, residual efficacy was specially showed until 28days after treatment whereas treatments with CS 30000 0.1% were similar to the control after 22days. These result show that the change of feedinng behavior and motrality of M. persicae is correlated with the change of nano type or non nano type of pyrifluquinazon.

본 연구는 부산물 혼합 silage 사료 급여가 한우 비육전기 거세우의 채식 및 반추행동에 미치는 영향을 규명하기 위하여 실시하였다. 시험에 사용한 공시한우는 21개월령 총 12두를 3처리로 배치하였으며, 이 때 처리구는 관행구 (C구: 농후사료+ 볏짚 자유채식)와 부산물 혼합 silage 구를 2처리 (T1: 농후사료+부산물 혼합 silage 제한급여 (1 kg) + 볏짚 자유채식, T2: 농후사료+부산물 혼합 silage 자유채식)로 하여 48시간

This study was performed to investigate the escape of pine wood nematode (PWN), Bursaphelenchus xylophilus, from two vector species (Monochamus alternatus and M. saltuarius) through oviposition and feeding behavior. First, we checked number of PWNs escaped from M. alternatus emerged from three different cases of pine logs. In case A, healthy pine trees were cut into logs and left in pine forest infected with PWN. In case B, healthy pine trees were cut into logs, left in large screen cage, and let them oviposited by M. alternatus emerged from pine trees infested with PWN. In case C, pine trees which were harboring M. alternatus were cut into logs, and PWN was inoculated artificially. The M. alternatus adults emerged from the above three cases of pine logs were checked in the next year to know how many PWN they were harboring in their bodies. The percentages of M. alternatus harboring PWN (18.3 and 15.6%, respectively) and number of nematodes per vector (5,713.1±9,248.3 and 2,034.1±4,746.8 PWNs, respectively) in case A and B logs are similar to each other. However, the percentage and the number in case C (38.3% and 20,083.1±32,188.3 PWNs) were higher than those of case A and B. Among 52 M. alternatus adults harboring PWN from all the three cases, 20 adults (38.5%) were harboring more than 5,000 PWNs per beetle. And these 20 adults were harboring 97.9% of the total PWNs in 52 adults. Second, we checked the daily escape of PWNs from M. alternatus and M. saltuarius collected at pine forest infested with PWN. The PWN escaped from their vector body for 34.9±12.4 days for M. alternatus, and for 23.9±16.2 days for M. saltuarius, reaching at peak escape during the 2nd week of emergence of the two vector species. A 44.5 and 47.2% to the total PWNs escaped from vector body within 2 weeks of vector emergence for M. alternatus and M. saltuarius, respectively. The number of PWNs escaped from each vector was 3,570.6±5,189.2 and 1,556.2±1,710.3 for M. alternatus and M. saltuarius, respectively.

To investigate resistance of Myzus persicae, which is resistant to etofenprox belonging to pyrethriods, to other insecticides, fenpropathrin and thiamethoxam, mortality and LC₅₀ values for these two insecticides against the etofenprox-resistant and -susceptible populations were obtained and EPG analysis for feeding behaviors of thiamethoxam or fenpropathrin treated and non-treated M. persicae was conducted. For fenpropathrin, mortality after 48 h treatment was 28 and 29%, respectively for the resistant and susceptible population. The LC₅₀ value was 193.15 and 93.46 ppm, respectively. For thiamethoxam, mortality after 48 h treatment was 87 and 57%, respectively for the resistant and susceptible population. The LC₅₀ value was 3.17 and 30.34 ppm, respectively. There was no significant difference in feeding behavior between fenprorpathrin treated and non-treated M. persicae. They showed a continuous feeding pattern. However, contact signal frequency increased in the thiamethoxam treated M. persicae 1 hour after treatment and significant difference was shown.

Host preference was tested on the 7 species plants against ggot-mae-mi, Lycorma delicatula (Hemiptera: Fulgoridae). This insect highly preferred Ailanthus altissima and Vitis vinifera however, didn"t choose the other plants preferentially. Both nymphs and adults lived longest in A. altissima and V. vinifera but lived in short and low ecdysis rate against other plants and 3 species fruits. By analyzing the phloem-feeding behavior using EPG, L. delicatula was showed the short time in non-probing phase and it also exhibit the longest feeding time in A. altissima and V. vinifera, but other plants did not feed the phloem at all. In sugar contents analysis, A. altissima existed high sucrose proportion and followed by fructose＞glucose, V. vinifera was analyzed by an order of glucose＞ fructose＞maltose＞sucrose＞rhamnose, Malus pumila was as glucose＞ fructose, Pyrus calleryana was as glucose＞unkown＞fructose, Hibiscus syriacus was as sucrose＞glucose. Nymphs and adults of L. delicatula lived longest in 5% sucrose solution, and next is in 5% fructose solution. However, they lived short in other sugar solutions. L. delicatula nymph and adult according to the combination of sugar proportion found in original plants lived longer in sugar combination solution of A. altissima and those of V. vinifera was next. Analyzed original sugar proportion from M. pumila, P. calleryana, H. syriacus respectively, L. delicatula lived short period comparing to the A. altissima, V. vinifera. This result was judged that sugar contents affected on choosing the host plants.

This study was performed to compare the host preference, survivorship and feeding behavior using EPG against ggot-mae-mi, Lycorma delicatula against seven plants such as Ailanthus altissima, Vitis vinifera, Malus pumila, Pyrus calleryana, Hibiscus syriacus, and Pinus densiflora. In host preference. L. delicatula was most preferred the Ailanthus altissima, Vitis vinifera and was not preferred the other plants. Survival rate of 3rd Nymph was recorded from Ailanthus altissima, Vitis vinifera as 15.0, and 15.4 days, respectively, it showed longest period. However, Malus pumila, Pyrus calleryana, Hibiscus syriacus, were survived within 6 days and Pinus densiflora was within 5 days. Moreover, L. delicatula was survived within 2 days to the three kinds of fruits. Ecdysis rate from 3rd to 4th nymph also high from Ailanthus altissima, Vitis vinifera as 63.3, 63.0 % and the order was followed as Malus pumila(17.7%) > Pyrus calleryana(9.3%) > Hibiscus syriacus(7.8%) > Pinus densiflora(5.9%). Especially, ecdysis rate was recorded 0% to the three kinds of fruits. Feeding behavior was analyzed using EPG and compared the differences their waveform from seven kinds of plants and three kinds of fruits. Non-probing time was short in host plants, reversely, Phloem-feeding time was recorded longer in Ailanthus altissima, Vitis vinifera as 45.7 and 13.7 min, respectively. And other plants and fruits were not showed feeding behavior.

This study was performed to investigate the correlation between changes of feeding behavior of green peach aphid, Myzus persicae and residual effect of an insecticide, Pyrifluzuinazon, using EPG technique. Pyrifluquinazon was showed the insecticidal activity until three days (72h) after treatment, and the activity was high in nymph than adult of GPA. There was no difference among treatment methods. Lethal sign was observed the slimed the abdomen of GPA after 3 days that rises the insecticidal activity, and dieㅇ as being sticked and/or pulled out the needle. Residual efficacy from 1, 3, 5, 7 to 20 days after treatment, insecticidal activity was showed 70% in 50 ppm, recommended concentration, until 5 days. And waveforms relating to non-probe time and phloem phase time using EPA, feeding inhibition efficacy was showed during 5 days after treatment, but showed similar level with control after 13 days. These results show that the change of feeding behavior of GPA is correlated with the change of residual effect of pyrifluzuinazon.

For the comparing of mortality of the resistance and susceptible population of Myzus persicae, etofenprox was treated in the recommended concentration of 200ppm. Mortalities of resistance population were 16.7 and 36.7%, and susceptible population were 86.7 and 86.7% after 24 and 48 hours treatment, respectively. For the detect of cross resistance to other pyrethroids, 6 pyrethroids were examined. Mortalities of susceptible and resistance populations were 90 and 31% to deltamethrin, 92 and 23% to lambda cyhalothrin, 81 and 14% to cypermethrin, 70 and 20% to α-cypermethrin, 29 and 28% to fenpropathrin, 84 and 29% to fenvalerate, respectively. It was showed that resistance populations were generally resistive to other pyrethroids. On the other hands, for recognized ecological characteristic of M. persicae susceptible and resistance populations life table was tested on the pepper leaves in the petri dish and on the plant in the pot. This results were showed that intrinsic rate of increase (r<SUB>m</SUB>), net reproduction number (R₀) and generation time in day (T<SUB>c</SUB>) were significantly different between two population in both tested. However, life span and reproduction period were slightly different between them. Otherwise, feeding behaviors were tested using EPG technique with non- and treated etofenprox. First potential drop time of susceptible and resistance population was 73.5 and 257.9 sec with non-treated and 93.3 and 1076.2 sec after treated, respectively. Electrical probing signals were 8.2 and 48.8 times with susceptible and resistance individuals after treated etofenprox, respectively. It was supposed that the resistance is more probings than susceptible population. After treated, total feeding time have more 6,728.9 sec on resistance than 965.5 sec on susceptible population. So, total non penetration time of susceptible population was 3,000 sec longer than resistance population.

Nymphal development of the B and Q biotypes of Bemisia tabaci was normal on all seven tomato varieties tested. However, their nymphal development was different on red pepper varieties. B biotype was not normally developed on nine red pepper varieties tested. On the contrary, Q biotype was normally developed, but its adult emergence rate was very low in Nokkwang variety than in other eight varieties. The EPG analysis of the feeding behavior of Bemisia tabaci showed that B and Q biotypes had different duration of phloem phases on red pepper. Q biotype showed longer phloem phases than B biotype. On Nokkwang variety, Q biotype had short phloem phases and did not prefer to feed on Nokkwang variety. Interestingly, Q biotype was found to have long duration of phloem phases on eight red pepper varieties, but B biotype did not prefer to feed on red pepper varieties. However, both biotypes did not show any difference in feeding time on tomato varieties.

This study was performed to investigate the change of probing and feeding behavior of Q type of B. tabaci according to the decrease of residual effect of two insecticide, emamectin and pyridaben, using EPG technique. Examined the residual activity during 20 days, pyridaben is showed longer than emamectin benzoate. When treated two insecticides onto tomato leaves with the recommended concentration through 20 days, EPG waveforms of Q type of B. tabaci was recorded during three hours compare with the characteristic patterns of feeding behavior between two insecticides such as duration of first probing time, total duration of non-probing phase, total duration of probing phase and total duration of phloem phase. Recorded result from the change in total duration of probing activity to react the two insecticides, pyridaben was showed higher the time of probing activity, however, total duration of phloem phase was appeared low activity. Total duration of phloem phase with passage of days did not show until seven days, however, and gradually increased in emamectin benzoate after 10 days. In conclusion, residual effect between two insecticides was showed more rapidly decreased in emamectin benzoate, however, feeding behavior of Q type of B. tabaci was increased.

This study was performed to investigate the feeding behavior of Bemisia tabaci B- and Q-biotypes using EPG technique against seven tomato and eight red pepper commercial varieties. EPG waveforms was recorded during three hours compare with the characteristic patterns of feeding behaviors between two biotypes such as total duration of non-probing times, the time taken until stylet activities changed after reaction, number of probes, and total duration of phloem phase. In comparing the effect of the varieties between the two biotypes, biotype Q showed the feeding behavior against all pepper varieties in the total duration of phloem feeding. Daeshin variety has the longest feeding time. However in total duration of phloem feeding, biotype B was observed in hanyeoreumbigarim and Daeshin varieties, but feeding time was very short. Biotype B was longer in total duration of non-probing, and showed lower number of probes, and total duration of probing phase. However, biotype Q was shorter time in total duration of non-probing phase than biotype B, but showed more aggressive probing and stylet pathway pattern. These results suggest that biotype Q are more preferred the pepper host than biotype B. However, tomato varieties between the two biotypes did not show the difference.

Resistance-breaking ability of wild brown planthopper (BPH), Nilaparvata lugens, on resistant rice cultivars has been reported in many Asian countries. To understand the development of resistance-breaking ability of wild BPH in Korea, we conducted the nymphal survivorship test and the electrical penetration graph (EPG) on susceptible and resistant rice cultivars with four different BPH populations, which were collected in the early 1980s (S-BPH), 2005, 2006, and 2007, respectively. The S-BPH showed low survival rates on resistant rice cultivars carrying Bph1 and bph2, respectively. On the other hand, recent(2005~2007) wild BPH populations seemed to have high resistance-breaking ability because they maintained elevated survival rates on most other resistant rice cultivars except Gayabyeo (Bph1 and bph2) and Rathu Heenati (Bph3). In the EPG monitoring, however, wild BPHs could not easily feed on the phloem sap of resistant rice cultivars, Cheongcheongbyeo (Bph1), ASD7 (bph2), and Gayabyeo. Wild BPHs spent more time on reaching the phloem sieve elements of resistant cultivars. Ph waveform duration and honeydew excretion amount of wild BPHs also decreased. From the results, we suggest that though recent wild BPHs collected in Korea have high resistance-breaking ability simultaneously on rice cultivars carrying Bph1 and bph2 through the increase of survivorship, they still have to pay some cost to feed on the phloem sap of resistant rice cultivars.

복숭아혹진딧물의 섭식행동을 관찰하기 위해, 고추, 무, 배추, 가지, 참외 등 5종류의 기주식물에서 EPG기술을 이용하였다. 복숭아혹진딧물의 구침이 식물체 표면에 접촉된 후 전기적 연결이 일어나기까지 소요되는 시간과 맨 처음 potential drop이 일어나기까지 소요된 시간, 구침의 세포간극내에서의 활동에 의하여 일어나는 potential drop의 수, 전기적 연결신호가 나타난 시점부터 체관부를 섭식하는 순간까지 소요된 시간 등을 조사한 결과 Potential drop 수에서는 기주들 사이에 유의성 있는 차이를 나타내었다. 그러나 나머지 요인에서는 기주식물들 사이에 큰 차이를 보이지 않았지만, 참외나 배추보다는 고추, 무, 가지에서 즘 더 기주선호성과 관련된 섭식패턴들을 확인할 수 있었다.

충남지방에서 많이 재배되고 10개의 벼 품종을 대상으로 벼멸구(Nilaparvata lugens)의 섭식행동을 EPG 패턴으로 분석하였다. 또한 벼의 섭식시간과 감로분비량의 관계를 알아보고 측정한 EPG 패턴을 비교분석하였다. 벼멸구의 각 EPG type을 전기적인 특성에 따라 1-6까지의 6가지로 분류하였다. 이중 Type 1은 구침을 꽂지 않고 쉬거나 돌아다닐 때 나타나는 패턴이고 Type 2는 체관을 흡즙할 때 나타나는 패턴이고 Type 3은 처음으로 구침을 꽂을 때, Type 4는 타액 분비시, Type 5는 물관의 흡즙시, Type 6은 구침의 세포내 이동시 나타난다. 벼멸구의 각 품종에 대한 섭식시간은 금남벼에서는 짧은 시간동안 이루어졌고 동전벼, 대안벼, 동안벼, 대산벼에서는 오랜 시간 흡즙을 하였다. 또한 금남벼에서는 장시간 구침을 삽입하지 않는 시간이 길었다. 감로의 분비량은 동진벼에서 가장 많았고 금남벼에서 가장 적은 감로를 분비했다. 이 감로의 분비량은 체관의 흡즙시간과 비례하여 나타났다. EPG type과 감로의 분비량을 분석한 결과 벼멸구가 선호하는 품종은 동진벼, 동안벼, 대산벼, 대안벼 등이었고 그렇지 않은 품종은 금남벼, 다산벼, 남천벼 등이었다.