키틴합성저해제인 diflubenzuron, triflumuron, chlorfluazuron을 갈색거처리와 유충과 번데기에 처리하여 효력검정법을 개발하였다. 유충에 대한 처리는 국소처리와 독먹이처리를 행하였고, 번데기에 대하요 용체침적처리와 미량주사처리를 행하였다. 유충에 대한 10 국소처리와 번데기에 대한 100ppm 용체 침적처리 결과 세약제 모두 유충 및 번덱의 발육에 아무런 영향을 주지 않았다. 유충에 대한 독먹이 처리에 있어서의 {{{{ { LC}_{ 50} }}}}값은 diflubenzuron 11.3, triflumuron 42.7, chlorfluazuron 6.2ppm이었다. 번데기에 대한 미량주사처리에서는 diflubenzuron 0.053, triflumuron 0.230, chlorfluazuron 0.340 /pupa의 {{{{ { LC}_{ 50} }}}}값을 나타내었다.

This study was conducted to examine the performance of poultry production and ammonia emissions from poultry litter when the mealworm (Tenebrio molitor L.) powder was fed to broilers and ducks. In Experiment 1, a total of 180 1-day-old broilers (Arbor acres) were allocated to two treatments with three replicates in a completely randomized design. In Experiment 2, ducks were used in the same method as in Experiment 1. The dietary treatments were as follows: basal diets as control and basal diets with 1.5% Tenebrio molitor L. powder as T1. In Experiment 1, broiler production was not affected by the addition of mealworm powder (p>0.05). Ammonia from broiler litter was observed significantly different in the two treatments at 4 and 5 weeks (p<0.05); however, in other weeks ammonia measured did not show significance different (p>0.05). In Experiment 2, feeding of mealworm powder had no statistical significance on duck productivity (p>0.05). Ammonia emissions from duck litter were not statistically significant in the two treatments at 2 to 5 weeks (p>0.05); however, there was a difference at 6 weeks (p<0.05). Therefore, the addition of mealworm powder to broiler and duck diets did not only improved weight gain and feed efficiency, but also effectively reduced ammonia in poultry litter.

This study was conducted to investigate the effects of adding Tenebrio molitor L Powder to broiler feed on fatty acid profiles in broiler breast meat. In total, 180 broiler chicks (1-day old Arbor Acres) were included. The birds were randomly divided into control and treatment groups, with 3 replicate subgroups each(30 birds per subgroup), and fed a diet for 35 days without (control) or with 1% Tenebrio molitor L powder (treatment). Among individual fatty acids, addition of Tenebrio molitor L. powder resulted in slightly higher C18:1n-9, C20:3n-3 and C20:3n-6 contents, and lower C18:2n-6 content compared withcontrols (p<0.05). No remarkable differences in total SFA and total USFA contents were found between groups. In conclusion, inclusion of Tenebrio molitor L. in broiler diets did not improve overall fatty acid profiles.

This study aimed to provide basic evidence regarding the development of materials by analyzing the physicochemical properties and antioxidant activities of Streptococcus thermophilus KCCM 3782 strain-fermented Cordyceps militaris grown on Tenebrio molitor. Among the solvents tested, DPPH radical scavenging activity was the highest in the hot water-extracted sample after 30 min of extraction. Moisture content decreased, whereas crude protein and fat content increased, after lactic acid bacteria-mediated fermentation. Sodium, magnesium, calcium, and zinc contents increased, with potassium levels attaining the maximum value, whereas free amino acid content decreased after the fermentation. Among Hunter's color values, a value increased to 66.7→149.92 after fermentation, whereas the L and b values decreased to 15.79→-15.75 and 54.45→0.01, respectively. Cordycepin content assay increased from 7.02 mg% to 8.66 mg%. The DPPH free radical scavenging activity of the fermented product was 91.92 EDA%, which was higher than that of the extract (84.69 EDA%). The ABTS free radical scavenging and superoxide dismutase (SOD) activities were also higher in the fermented products.

This study was carried out to investigate the physicochemical and antioxidant properties of fermented milk with addition of hot water extract of Cordyceps militaris grown upon Tenebrio molitor. The general components of Cordyceps militaris grown upon Tenebrio molitor are moisture 10.85%, crude protein 18.44%, crude fat 2.07%, crude ash 5.46%. The DPPH radical scavenging activities of different solvents were the highest (74.81 EDA%) with hot water extract sample. The acidity of fermented milk was high with increasing amount of extracts. The pH of fermented milk reached 4.60-4.66 after 4 h of fermentation, and the number of lactic acid bacteria was highest (11.70 log CFU/mL) with 1% fermented milk. The moisture content of fermented milk showed no significant difference. In addition, contents of crude protein were not significantly different according to addition amount. Regarding Hunter’s color values, L value decreased as the amount of extract increased, whereas a value and b value increased. The content of free amino acid increased with increasing amount of extract. The DPPH free radical scavenging ability and ABTS free radical scavenging ability of fermented milk were significantly different as the addition amount of extract increased. In the sensory evaluation, fermented milk containing 1% of extract showed the highest preference.

국제연합식량농업기구(FAO)는 2050년, 전 세계 인구 수가 약 90억에 이를 것으로 예측하였다. 이와 같은 인구 증가는 여러 식량 및 환경 문제들을 야기할 수 있다. 또한 식량 및 에너지원 생산과정에서 자연계에 과잉으로 배출되는 질소, 인은 토양 산성화 및 부영양화 등의 환경문제를 유발할 수 있다. 이에 대한 해결책으로 FAO에서는 ‘곤충’을 자원(식･약품, 사료, 비료 등)으로 활용하는 것을 제시하였다. 현재 국내 연구는 곤충의 이용가능성 여부 및 활용기술개발 위주의 연구에 치중되어있어 곤충활용에 따른 환경성 연구는 전무한 실정이다. 따라서 본 연구에서는 국내에서 식용곤충으로 인정받은 곤충 6종 중 갈색거저리와 벼메뚜기를 선정하여 곤충의 활용방안에 따른 환경성 평가를 진행하였다. 갈색거저리는 최근 식량자원으로써 각광받고 있는 종이며 번데기까지 사용할 수 있는 완전변태 곤충이다. 벼메뚜기는 예로부터 식품으로 이용된 친숙한 종이며 대표적인 불완전변태 곤충이다. 본 연구의 목적은 곤충 분류(번데기의 유무)에 따른 활용방안의 환경성을 비교･평가하고 이를 기존 단백질원과 비교하는 것이다. 갈색거저리와 벼메뚜기의 환경성 평가는 질소, 인 흐름분석 및 전과정평가를 사용하였다. 이는 인간이 곤충을 단백질원으로 섭취하였을 경우와 곤충 사료로 키운 가축을 섭취하였을 경우로 나누어 분석하였다. 이를 토대로 물질흐름의 각 단계를 비료, 작물, 곤충, 가축, 사람으로 나누어 유입, 유출되는 질소와 인의 양을 산정하고 이에 따른 질소, 인 이용효율을 산정하였다. 또한 갈색거저리와 벼메뚜기 사육 시 발생하는 온실가스 량을 기존의 단백질원 생산과 비교 하였다.연구 결과 갈색거저리가 기존 단백질원인 한우와 벼메뚜기보다 질소, 인이용효율이 높았으며 온실가스 발생량이 적었다. 또한 갈색거저리는 번데기까지 사료로써 이용이 가능하기 때문에 활용범위가 넓을 것이라 생각한다.

The purpose of this paper is to investigate potential anti-inflammatory and anti-oxidant effects of Tenebrio molitor. Macrophage cell response by outside stimulation leads expression of pro-inflammatory cytokines, such as tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1β), and trigger expression of genes which are affected by inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), resulting in formation of inflammatory factors like nitric oxide (NO) and Prostaglandin E2 (PGE2). Cell viability was determined by MTT assay. In order to investigate anti-inflammatory agents, the inhibitory effects on the production of lipopolysaccharide (LPS)-induced NO in RAW 264.7 cells were examined. T. Molitor significantly decreased the production of NO in a dose-dependent manner, and also reduced the expression of iNOS, a COX-2 protein. As a result, the levels of protein such as PGE2, iNOS, COX-2 and MARKs were significantly reduced compared to non-treated group in T. Molitor water extract (TDW) treated group. Also, antioxidant effect of T. Molitor were investigated using DPPH, ABTS+ and superoxide anion radical scavenging activity tests in cell-free system. Antioxidant activity of T. molitor was found low in the DPPH radical scavenging test while high in the ABTS+ and superoxide anion radical scavenging activity tests. These results show that TDW could be an effective anti-pro-inflammatory and anti-oxidant agent.