솔수염하늘소는 소나무에 시들음 증상을 유발하여 괴사시키는 소나무재선충을 매개하는 대표적 매개충이다. 소나무재선충병 고사목 방제 살충처리 방법으로 훈증방법을 대체할 수 있는 그물망을 이용한 방제법이 도입되고 있다. 본 연구는 솔수염하늘소의 쏠기와 탈출 행동을 유발시 키는 조건을 구명하기 위하여 구속 공간 및 온도를 달리 처리하고 탈출여부를 조사하였다. 솔수염하늘소는 직경 30 mm 이하의 공간에 구속되었 을 때 장애물을 뚫고 탈출이 가능하였다. 온도 15°C에서는 탈출에 성공하지 못하였으며, 20~30°C 범위에서 탈출 성공률이 높았다. 먹이를 이용 한 솔수염하늘소의 유인은 탈출 성공률에 영향을 미치지 않았다. 좁은 공간에 구속되지 않는 경우는 갉는 부위가 집중되지 않아 탈출공을 형성하 지 못하였다. 구속된 공간에서 솔수염하늘소는 가운데와 뒷다리 부절을 이용해 좁은 공간에서 몸을 이동시키며 큰턱으로 정면에 있는 장애물을 집중적으로 갉아서 탈출공을 만들고 앞다리를 뻗어 몸을 지탱하면서 빠져나오는 행동을 보였다. 본 연구의 결과는 훈증 대체기술로 제시되고 있 는 구속 그물망의 성능을 평가하는데 중요한 기초자료로 활용될 수 있을 것이다.

Monochamus alternatus (M. alternatus) and Monochamus saltuarius (M. saltuarius) are major vectors for Bursaphelenchus xylophilus in South Korea. When an adult, they are easily distinguishable by several morphological classification. However, it is difficult to identification between M. alternatus and M. saltuarius when they are larvae as they have very similar morphological characters. Thus, they are not easily distinguishable without expertise about Cerambycidae taxonomy. Furthermore, during epidemiological investigation, sometimes, adults or larvae would not be founded in death pine trees. For these reasons, in this experiment, we are able to identified between M. alternatus and M. saltuarius by mitochondrial 12S rRNA gene primers that are specific to 12S rRNA gene fragment of M. alternatus using larvae tissue and frass. Moreover, we had examined whether vectors that were already escaped from dead pine tree have Bursaphelenchus xylophilus or not by multiplex PCR using larva frass that was remained in dead pine tree.

The adult activity of M. alternatus caught in a pheromone trap showed a bimodal form consisted of the first peak in mid to late June and the second peak in mid to late September in Jeju area, Korea. The two peaks were separated apparently between mid and late August, showing a valley. The pine trees without oleoresin flow were abundant during early July to early August, and declined thereafter, which did not match with the valley of adult activity curve. Thus, the hypothesis that dying pine trees attract much strongly M. alternatus adults than that of pheromone lures may not fully explain the bimodal pattern, because the first adult activity peak occurred during the peak period of dying pine trees and it declined with decreasing dying pine trees. The accumulated degree-days showed that the emergence of the 2nd generation adults could occur before the second peak when the diapause ecology of M. alternatus was not considered. The voltinism of M. alternatus can affect basically the control strategy of this pest. Consequently, further studies are required to find out clearly the voltinism of M. alternatus in Korea.

Monochamus alternatus (M. alternatus) and Monochamus saltuarius (M. saltuarius) are major vectors for Bursaphelenchus xylophilus in South Korea. When an adult, they are easily distinguishable by several morphological classification. However, it is difficult to identification between M. alternatus and M. saltuarius when they are larvae as they have very similar morphological characters. Thus, they are not easily distinguishable without expertise about Cerambycidae taxonomy. Furthermore, during epidemiological investigation, sometimes, adults or larvae would not be founded in death pine trees. For these reasons, in this experiment, we are able to identified between M. alternatus and M. saltuarius by mitochondrial 12S rRNA gene primers that are specific to 12S rRNA gene fragment of M. alternatus using larvae tissue and frass. Moreover, we had examined whether vectors that were already escaped from dead pine tree have Bursaphelenchus xylophilus or not by multiplex PCR using larva frass that was remained in dead pine tree.

We had evaluated of insecticidal effect from Insecticide-treated net (ITN) that coated by deltamethrin against Monochamus alternatus which serves as a vector for Bursaphelenchus xylophilus. In this experiment, vector has been shown that rapid insecticidal effect response time within 1 hour when they were contacted with ITN. In addition, vectors were showed high mortality within 48 hours. To evaluate insecticidal persistency of ITN and whether releasing insecticide to water, ITNs were soaked in water over various time periods. Water has not shown insecticidal effect to vectors and small amount of insecticide were detected that would not be harmful to other non-target organic being such as honeybee. Also, reduction of insecticidal effect was not observed from the ITN. Taken together, our results suggest that ITN had got highly insecticidal effect to vector as well as could be applied to effectively prevent dispersal of the vector on the field.

Since first reported in 1988 the Pine Wilt Disease has been established and spreaded nearly nationwide in South Korea, causing tremendous economic damage in pine forests. The pine wilt nematode (Bursaphelenchus xylophilus), causing the Pine Wilt Disease, in Korea is known to be transported by two insect vectors that are Monochamus alternatus Hope and Monochamus saltuarius Gebler. Currently the pest management strategy largely relies on pesticide application and infected tree clearing. The aim of the present study was to search for early larval parasitoids of M. alternatus using the sentinel log infested with the cerambycid eggs. Only one braconid species was collected and identified as Spathius verustus Chao. Two to ten wasp larvae attached and fed on one host larva. Parasitism rate was 55.5 to 59 % in Jinju site, while 1 to 18.1 % the other sites. Sex ratio was female-biased (female : male = 18.9 : 1). The S. verstus has potential to be use as parasitic natural enemy of M. alternatus.

Monochamus alternatus is one of the insect vector of pine wilt disease with Monochamus saltuarius. The present study aimed to investigate the dose to induce sterile and the effect of sterility according to the gender of M. alternatus. As the electron beam irradiation dose increased, the longevity of the mid and late maturation feeding stage of adults was slightly reduced. However, there was no difference between these two adults. In addition, female adults were more sensitive to the electron beam than the male adults. Hatchability of the F1 generation of ♂T×♀N or ♀T×♂N (T: 200 Gy electron beam irradiation, N: 0 Gy) was completely suppressed. As electron beam irradiation dose increased, the fecundity of female adults were slightly decreased. Results by comet assay showed that electron beam irradiation induced as dose increased DNA damage in M. alternatus adults. These results suggest that SIT using electron beam may be useful for control of M. alternatus.

소나무재선충병에 대한 방제 전략 측면에서 한정된 인력과 자원을 효과적으로 이용하려면 방제 대상 지역에서의 소나무재 선충병 피해 확대 방향과 발생 가능 지역을 예측하여 대비할 필요가 있다. 본 연구에서는 소나무재선충병 확산 예측을 위하여 솔수염하늘소(M. alternatus)와 환경조건을 이용한 CLIMEX, MB Index, Maxent 모형으로 분석하였다. CLIMEX 분석을 위하여 일기상 자료(최고기온, 최저기온, 강수량, 평균 상대습도)를 월별 10년 평균하여 68개 관측지점별로 구축하였다. 미래 예측 자료는 기상청 기후변화정보센터에서 제공하는 12.5km 격자의 월별 자료(RCP 8.5)를 10년 단위 평균값을 도출하고 68개 관측지점별로 추출하였다. MB Index와 Maxent 분석을 위한 입력자료는 소나무재선충병 발생 지점, 월평균기온과 지형(고도, 향, 경사)을 이용하였다. 소나무재선충병 확산과 관련한 3가지 모형에서 도출된 결과를 종합한 결과, 2020년대에는 전라남도와 경상남도, 충청남도 내륙지역과 수도권을 중심으로 확산할 것으로 나타났다. 2050년대에는 강원도 백두대간 지역을 제외한 우리나라 전역으로 소나무재선충병이 확대될 것으로 예측되었고, 2090년대에는 전국적으로 소나무재선충병 이 발생할 것으로 나타났다.

Many forest pests present a problem during wood quarantine. The effectiveness of phosphine (PH3), which is an altered form of MB, was examined in Monochamus saltuaris and M. alternatus. After 24 h, the larvae and the adults of both insects were susceptible to PH3 at 3 mg/L at 20°C. However, the larvae of both pests were rather than dead, seem to have been quiescent condition. PH3 showed increasing insecticidal activity in a time-dependent manner in the larva stage of both pests. We investigated the fumigants at all stages of M. saltuaris and M. alternatus, as well as the synergistic effects of PH3 in controlled atmospheres of 50% and 80% oxygen. The atmospheric oxidation of PH3 fumigation slightly increased the toxicity of the fumigant to larva stages in 125 L container at 4 mg/L. These results indicate that highly concentrated atmospheric oxidation of PH3 could be useful as a fumigant agent against M. saltuaris and M. alternatus.

The pine wilt disease that blocks the path for water and nutrition in pine trees is caused by the nematode, Bursaphelenchus xylophilus (Nematoda: Aphelenchoididae). The nematode relies on the longhorn pine sawyer beetle Monochamus alternatus and Monochamus saltuaris (Coleoptera: Cerambycidae) as vectors. Recently, 2-(undecyloxy)ethanol was identified as a male-produced aggregation pheromone of Monochamus species. In this study, we investigated the effect of 2-(undecyloxy) ethanol along with host plant volatiles -pinene and ethanol on attracting M. alternatus at a pine forest in Pohang, Korea from May, 2014 to July, 2014. To sustain the volatility of 2-(undecyloxy)ethanol and host plant volatiles, a superabsorbent polymer based on polyacrylic acids and water were added to the pheromone mixture. A total of 46 M. alternatus were collected from two field bioassays. Our results indicate that 2-(undecyloxy)ethanol is effective in attracting M. alternatus in Korea. Our study suggests that the aggregation pheromone could be used for detection and population monitoring of the beetles as well as for the effective mass trapping in outbreak situations.

Thermal death kinetics of the Japanese pine sawyer, Monochamus alternatus, was investigated to develop a heat treatment method that controls the pest in wood packing materials used in the export of goods. To determine the feasibility of microwave irradiation as an alternative treatment, laboratory experiments were performed on blocks (200x200x250 mm) of Douglas fir artificially infested with live larvae and pupae irradiating the blocks with 2.45 GHz of microwave energy. One hundred percent mortality of larvae and pupae of Japanese pine sawyer was observed by hot water treatment at 64°C and 68°C, respectively. One hundred percent mortality of treated Japanese pine sawyer was achieved above 67°C for larvae and 70°C for pupae based on the measured wood temperature, regardless of the irradiation time.

우리나라 소나무재선충병(Pine Wilt Disease)은 1988년 부산 금정산 지역을 중심으로 최초 발생된 이후 최근까지 피해지역이 전국적으로 확산되고 있는 실정이다. 크기가 1mm 미만의 실처럼 생긴 소나무재선충(Bursaphelenchus Xylophilus)이 소나무의 수분과 영양 이동 통로인 가도관을 막아 소나무를 말라 죽게하는 병으로, 선충 스스로는 이동할 수 없고 반드시 매개충(carrying insect) 이동에 의해서만 감염이 되기 때문에 솔수염하늘소 성충이 건전한 소나무 가지의 신초를 갉아먹는 후식(Maturation feeding)을 할 때와 산란을 할 때 생기는 수피의 상처 부위를 통해서 건전한 나무로 옮겨진다. 소나무류는 재선충에 한번 감염되면 대부분 3개월 이내에 완전 고사되며 매개충은 이들 고사목에 다시 서식하는 공생관계(symbiotic relationship)를 유지하기 때문에 소나무재선충병의 발생 및 확산은 소나무 임분내 입지환경 및 임분조성, 매개충의 서식환경이 밀접한 관계를 가진다. 따라서 소나무재선충병 피해임지와 피해발생 우려지역의 소나무림 임분구조 분석과 임분상태를 파악하고 간벌 방법별 매개충 서식밀도와의 관계를 구명함으로서 생태적으로 안정되고 건전한 소나무 임분관리, 소나무재선충병 확산방지에 적합한 임업적방제 임분시업 기술 개발을 위하여 입지환경에 따른 임분시업별 실연시험지를 2006년과 2007년에 각각 2지역에 조성하였다. 추기와 춘기에 간벌된 지역에서의 간벌목에 대한 매개충 침입공수는 춘기 처리구가 63개로 가장 많았으며 산란 개체목에서의 성충 탈출공 발생수 역시 46개로 가장 높았다. 간벌시기별 매개충 유충 발생율은 춘기가 평균 35.8%로 가장 높았으며, 추기 간벌기인 11월과 10월 처리구가 각각 20.0, 14.8% 순으로 조사되었다. 또한 산란 개체목 경급별 유충 발생율은 15cm이상에서 53%로 가장 높게 나타났다. 임내 산물 위치별 매개충 서식 밀도는 산록부가 39.5%, 산복부 35%, 산정이 25.0%순으로 산록부에 매개충의 산란이 높은 것으로 조사되었다. 따라서 간벌시기에 따른 매개충의 산란은 춘기 처리구에서 높은 유충밀도 및 피해목 발생을 보이고 있어 향후 소나무재선충병 피해지 주변 선단지의 임분시업을 위해서는 춘기보다는 추기에 실시하는 것이 효과적 일것으로 사료된다.

소나무재선충병(Pine Wilt Disease) 방제법은 2000년대까지는 피해목을 벌채하여 소각하는 방법을 주로 이용하여 왔으나 방제물량이 많아지고 밀생임분에서의 소각 공간 확보가 어려워 열해목이 발생하고 이동시에는 피해목을 완벽하게 수집할수 없는 문제점이 있었다. 이를 보완하기 위하여 최근에는 소나무재선충병 감염목을 이동할 필요가 없고 현장에서 매개충인 솔수염하늘소 방제가 가능한 훈증 방제법이 보급되어 주력 방제법으로 활용되고 있다. 그러나 훈증약제는 환경오염이나 인축에 피해가 있을 뿐 아니라 약제투입 약량에 따라 매개충이 피복소재를 뚫고 탈출할 수 있고 유효 천적도 동시에 제거되며 경관도 해치는 문제가 있다. 따라서 농약을 투입하지 않고도 매개충의 탈출을 효과적으로 제어할 수 있으며 천적 등의 역할을 증대시키고 수분 유입 등으로 벌채목의 부패를 촉진시키어 매개충의 재 산란을 방지할 수 있음은 물론 경관도 크게 해치지 않는 소나무재선충병 방제 피복망을 선발 하였다. 피복망의 소재는 Multi filament 22합수 소재가 가볍고 저렴하며 재활용이 가능하므로써 매개충 방제에 가장 적합한 것으로 조사되었으며 피복 후 1주일내에 매개충 90%이상이 치사되었고 이후에도 전혀 탈출이 불가능하였다. 또한 처리 후 2년이 경과하여도 망 훼손 및 매개충 탈출 흔적은 찾아 볼수 없었고 오히려 벌채목의 부패가 촉진되어 매개충 재 산란의 위험이 해소되었다. 제형으로는 벌채된 소나무량에 따라 현장에서 크기에 맞게 절단 피복 할 수 있는 두루마리(Roll) 형태가 가장 효율적이었다. 대량 생산시 망 크기, 제형 등을 수요자 임의로 조정할 수 있는 장점을 가지며, 기존 훈증방제법에 비하여 약 20%이상의 예산 절감 효과가 기대될 뿐만 아니라 환경 및 천적보호에도 기여 하므로서 소나무재선충병 방제에 효율적으로 활용이 가능하다

Trunk injection of nematicides was applied to prevent pine wilt disease (PWD). Although the trunk injection of nematicides was effective to PWD prevention, it was not effective to a vector, Monochamus alternatus. Thus, This study was investigated for the insecticidal activity and the effect of oviposition deterrence by injecting to pine trees with systemic insecticides such as Acetamiprid SC 10%, Imidacloprid DC (20%), and Thiamethoxam DC (15%). As a results, mortality of M. alternatus adults was 100% at 56 days after trunk injection. Percentage of M. alternatus adults moved to a young black pine tree by trunk injection of Imidacloprid DC (20%) and Thiamethoxam DC (15%) in screen cage (4.0×2.0×2.5m) was 76.7% and 70.0%, respectively. But the mortality of M. alternatus adults showed 100%. Percentage of M. alternatus adults moved to the treated and untreated young black pine trees by trunk injection of Acetamiprid SC (10%) in screen cage (4.0×2.0×2.5m) were 25.9% and 49.5% at 1st day and 3rd day after treatment, respectively. Percentage of M. alternatus adults oviposited to treated pine logs at 3month after trunk injection of liquid mixture of Acetamiprid SC (10%) and Emamectin benzoate EC (2.15%) in screen cage (72×72×100㎝) was 25%. But, untreated pine logs was 100%

Movement of adult Monochamus alternatus was investigated on young black pine, Pinus thunbergii, trees for about one month in a screen cage. Number of pine trees to which M. alternatus females and males moved during the experimental period was 6.4±1.4 and 7.2±1.5 out of 15 trees, respectively. Adult females and males moved to 0.2±0.1 and 0.3±0.1 tree per day, respectively. A 74.6 and 80.7% of adult females and males moved at night (17:00-08:00) to other trees. After moving, most beetles (72.6% of females and 76.0% of males) stayed on the tree to which they moved for one to two days. Some beetles stayed for up to seven days.

Cuticular hydrocarbons (CHCs) of the pine sawyer (Monochamus saltuarius), Japanese pine sawyer (M. alternatus) and oak longicorn beetle (Moechotypa diphysis) were analyzed by GC, GC-MS and compared. Monochamus beetles are typical vectors of pine wilt disease but Moechotypa diphysis, which belongs to the same family, is not. They possess different CHCs in carbon number: 23-25 in M. saltuarius, 25-32 in M. alternatus, and 23-29 in M. diphysis. In comparison to inter-species, these three species of adult beetles have different numbers and chains of constituents of CHCs. In comparison between male and female in intra-species, the quantities of CHCs show the difference but constituents are not. Major constituent of M. saltuarius were analyzed as n-pentacosane ＞ n-nonacosane ＞ n-heptacosane, those of M. alternatus were n-nonacosene ＞ n-pentacosane ＞ n-nonacosane, and those of M. diphysis were n-heptacosane ＞ 13-methylheptacosane ＞ 3-methylheptacosane. From the body surface, most saturated carbohydrates of 3 species beetles are composed of n-alkane (40.2 - 65.7%) and followed by olefines ＞ monomethylalkanes that one or two double bonds in M. saltuarius and M. alternatus. Otherwise, M. diphysis have the difference in order of monomethylalkanes > olefins.

This study was performed to investigate the escape of pine wood nematode (PWN), Bursaphelenchus xylophilus, from two vector species (Monochamus alternatus and M. saltuarius) through oviposition and feeding behavior. First, we checked number of PWNs escaped from M. alternatus emerged from three different cases of pine logs. In case A, healthy pine trees were cut into logs and left in pine forest infected with PWN. In case B, healthy pine trees were cut into logs, left in large screen cage, and let them oviposited by M. alternatus emerged from pine trees infested with PWN. In case C, pine trees which were harboring M. alternatus were cut into logs, and PWN was inoculated artificially. The M. alternatus adults emerged from the above three cases of pine logs were checked in the next year to know how many PWN they were harboring in their bodies. The percentages of M. alternatus harboring PWN (18.3 and 15.6%, respectively) and number of nematodes per vector (5,713.1±9,248.3 and 2,034.1±4,746.8 PWNs, respectively) in case A and B logs are similar to each other. However, the percentage and the number in case C (38.3% and 20,083.1±32,188.3 PWNs) were higher than those of case A and B. Among 52 M. alternatus adults harboring PWN from all the three cases, 20 adults (38.5%) were harboring more than 5,000 PWNs per beetle. And these 20 adults were harboring 97.9% of the total PWNs in 52 adults. Second, we checked the daily escape of PWNs from M. alternatus and M. saltuarius collected at pine forest infested with PWN. The PWN escaped from their vector body for 34.9±12.4 days for M. alternatus, and for 23.9±16.2 days for M. saltuarius, reaching at peak escape during the 2nd week of emergence of the two vector species. A 44.5 and 47.2% to the total PWNs escaped from vector body within 2 weeks of vector emergence for M. alternatus and M. saltuarius, respectively. The number of PWNs escaped from each vector was 3,570.6±5,189.2 and 1,556.2±1,710.3 for M. alternatus and M. saltuarius, respectively.

Trunk injection of nematicides is applied to prevent pine wilt disease(PWD). Although the trunk injection of nematicides is effective to PWD prevention, it is not effective to vector insects such as adults of M. alternatus and M. saltuarius. Thus, This study was investigated for the insecticidal activity and the effect of feeding deterrence by injecting to pine trees with acetamiprid. For screening effective nematicides, some insecticides were injected to pines on December 30th, 2008 and their insecticidal activities and feeding areas of M. alternatus and M. saltuarius were investigated in the insects breeding dishes (100× 40mm) where put on 1 to 2-year-old twig which was selected from injected pine trees in early June. As a result, insecticidal activity and feeding deterrence of acetamicprid were most effective. Feeding areas of M. alterantus adults, when tested with 1-year-old twig and 2-year-old twig of healthy pine trees, were 170.3±107.0 mm2 and 258.0±123.2 mm2 respectively. And Feeding areas of M. alterantus adults, when tested with 1-year-old twig and 2-year-old twig of pines injected at a dose of 0.3 ml per cm dbh of a pine tree with acetamiprid, were 57.7±72.3 mm2 and 65.7±51.2 mm2 respectively and their mortalities also were 0%, 28.6%. The feeding areas of M. alterantus adults tested with 1-year-old twig and 2-year-old twig of pine trees injected at a dose of 0.6 ml per cm dbh of a pine tree with acetamiprid, were not fed on it and the mortalities were 28.0%, 57.1% respectively. In case of the feeding areas of M. saltuarius adults, when tested with 1-year-old twig and 2-year-old twig of healthy pine trees, it was 112.±35.1 mm2 and 159.2±65.2 mm2 respectively. And the feeding areas of M. saltuarius adults, when tested with 1-year-old twig and 2-year-old twig of injected pine trees at a dose of 0.3 ml per cm dbh of a pine tree with acetamiprid were 0.61±0.0 mm2 and 8.1±12.4 mm2 and both mortalities were 23.1%. Although M. saltuarius adults, when tested with 1-year-old twig of a pine tree injected at a dose of 0.6 ml per cm dbh of a pine tree with acetamiprid, were not fed on it but M. saltuarius adults tested with 2-year-old twig of the same pine tree were 2.0±3.7 mm2 and their mortalities were 53.8%, 61.5% respectively. We investigated insecticidal activities, the effects of feeding deterrence of acetamiprid for vector insects where fed twigs of injected pines by stop up hole with a cork or not, and the trunk injection to pine trees at a dose of 0.5, 1 ㎖ per ㎝ dbh of a pine tree with acetamiprid on the early June. As a result, all of the vector insects were not fed and the feeding area was within 11 ㎟ and the mortality of M. alternatus was 70%.

Pine wood nematode, Bursaphelenchus xylophilus, is a causative organism to induce pine wilt disease in many varieties of pine trees. Until 2006, Monochamus alternatus had been known as the only insect vector of pine wood nematode in Korea which targeted on Pinus densiflora (Japanese red pine) and P. thunbergii (Japanese black pine). However, pine wilt disease was also reported from Korean pine tree (Pinus koraiensis) in 2006 and we found another insect vector, M. saltuarius, was involved to transmit pine wood nematode. Both Monochamus species were confirmed to transfer pine wood nematode to their hosts but, there is no detail information about other transmitted nematode. Especially Bursaphelenchus mucronatus is common species transmitted by Monochamus species which is morphologically closed to B. xylophilus. Moreover B. mucronatus have two genotypes; one is East Asian type and the other is European type. Both genotypes of B. mucronatus were found in Korea but, the host and vector information related to the genotypes of B. mucronatus was not clear. Monochamus saltuarius was collected from three different geographical locations and nematodes were extracted and identified. For the identification of the juveniles, nematode DNA was extracted and ITS-RFLP analysis was done by PCR and gel electrophoresis. The selected enzymes were Hinf I, Alu I, Msp I, Hae III, Rsa I. Most of Bursaphelenchus species carried by M. saltuarius, which collected from pine wilt disease-free area, was determined as European type of B. mucronatus. We will compare the nematode species and genotypes carried by M. alternatus and M. saltuarius. In addition the rate of nematode carrying insect and the average number of nematode per single insect will be counted and compared.

Monochamus alternatus and M. saltuarius were reported as the vectors of Bursaphelenchus xylophilus, pine wood nematode in Korea. According to Kwon et al. (2006), each of 2 species has occupied their own regional distribution : M. saltuarius in southern part including Jeju island and M. alternatus in mid-northern part of Korean peninsula. We measured the supercooling point (SCP) of 2 species (laboratory-reared populations) by each of developmental stages. The SCPs of 2nd, 3rd and 5th instar larvae of M. saltuarius were -7.68±0.19℃, -7.02±0.69℃, -4.93±1.34℃ each of stages. On the other hand, the SCPs of 3rd, 4th, 5th instar larvae and pupae of M. alternatus. were -4.46±1.12℃, -5.94±1.33℃, -7.83±1.44℃, -9.53±1.78℃ each of stages. The SCPs of M. saltuarius larvae generally was lower than that of M. alternatus. The pupae of M. alternatus and 2nd instar larvae of M. saltuarius had the lowest SCP among measured samples. On the other hand, the highest SCP were recorded in 2nd and 5th instar larvae, each. This result shows that regional distribution of 2 beetles may be associated with the adaptation capacity to low temperature represented by the SCP as well as the developmental temperature. However, beetles experimented were not collected from pine forest fields. In further study, we are planning experiments with field populations and all developmental stages.