The cotton aphid, Aphis gossypii (Glover), is one of the most serious pest in the cultivation of various vegetables. A highly imidacloprid-resistant field population (CA-L) was collected from cucumber at Gangwha island in 4th August 2011. Even though neonicotinoid insecticides especially imidacloprid were sprayed six times during June and July, aphid density was too high to be counted. IEF and 2DE analyses revealed that general esterase isozyme (pI. 5) in CA-L were dramatically overexpressed and more isozyme spots identified in CA-L compared to susceptible (CA-S) strain. To identify differentially expressed genes in CA-L, comparative transcriptome analyses based on GS-FLX were conducted with total RNA extracted from CA-L, which generated ca. 143 Mb reads. Previously reported, comparative transcriptome analyses performed in imidacloprid resistant (CA-IR) and CA-S. The comparative transcriptome analyses re-investigated after all data sets were combined together. As previously reported, seven ATP-binding cassette (ABC) transporter genes were newly identified in A. gossypii, among which only ABCC9 gene was highly expressed in CA-IR and L. These results suggested that ABCC subfamily associated with imidacloprid resistance in A. gossypii.

After pea aphid Acyrthosiphon pisum genome project, hologenome concept was applied to pea aphid and symbioant such as Buchnera aphidicola. Here we screened symbiotic microorganism in four lab strains (two genetically different insecticide susceptible strains, host plant: cucumber and two different host adopted imiacloprid resistance strains, host plants: cucumber and potato) and four field populations (Jeju, Goryeong, Gimjae and Muju, host plant: potato) of cotton aphid based on GS-FLX pyrosequencing which were conducted with universal primer amplified partial fragments of 16S rRNA from total DNA which was extracted from each strain and population. B. aphidicola occupied over 90% of all identified prokaryotic microorganisms which all tested samples. It’s interesting that the ratio of B. aphidicola occupied over 99% in all of the tested lab strains. However, specific enterobacteriaceae occupied six to seven percents of all field populations which closely related endosymbiont of Glycaspis brimblecombei. That means B. aphidicola occupied only 91~92% of all identified prokaryotic microorganisms. Futhermore, other actinobacteridae and bacillaceae also were detected in field populations. The results obtained for these ratios suggested that there has some interaction between symbioant and environment NOT in imidacloprid resistance.

노즐시험기로 측정되는 직물의 당김 힘은 직물이 갖는 포괄적인 촉감을 나타낸다. 노즐시험기는 기존 KES 시스템보다 저가이며, 측정에 있어서 절차가 간단하고 빠르다. 본 연구에서는 노즐시험기로 측정되는 직물의 촉감을 정량적으로 평가하기 위해 마찰법칙을 기초로 하여 반 실험적 촉감 방정식을 개발하였다. 모델 식에서 고려된 변수들은 직물의 마찰계수, 그리고 직물의 굽힘 길이와 전단 신도에 의해 결정되는 직물의 3차원적 변형 특성을 나타내는 드레이프 계수이다. 12 종류의 서로 다른 물성의 면과 폴리에스터 직물들을 사용하여 실험 측정한 촉감 값과 모델 방정식으로부터 예측한 촉감 값들을 비교 분석하였다. 높은 마찰계수, 높은 굽힘 길이, 그리고 낮은 전단 신도를 갖는 직물일수록 높은 촉감 마찰력, 즉 낮은 촉감 성능을 보여주었다. 실험 측정한 촉감 값과 모델 식으로부터 계산한 값들 사이 잘 일치하였으며, 이와 같은 결과는 모델 식을 사용하여 직물의 고유 물성들로써 촉감의 객관적인 예측이 가능함과 더불어 촉감 성능의 향상을 위한 직물설계에 있어서도 유용한 정보를 제공할 수 있음을 보여준다.

The cotton aphid, Aphis gossypii (Glover), is one of the most serious pest in seed potato and various vegetable cultivation. The imidacloprid-resistant strain (IR) was over 200-fold more resistant to imidacloprid compared to a susceptible strain (S) as judged by LC50 values. The IR showed cross resistances to other neonicotinoid insecticides. IEF and 2DE analyses revealed that general esterase isozyme patterns in IR were almost identical to those of S. Nevertheless, a significantly overexpressed protein spot was detected in IR. To identify differentially expressed genes in IR, comparative transcriptome analyses based on GS-FLX were conducted using total RNAs extracted from both IR and S strains, which generated ca. 290 Mb reads for each strain. Generally, most nicotinic acetylcholine receptor subunit genes, such as alpha 2 and beta 1, were more transcribed in S than in IR. In contrast, only alpha 5 subunit gene was 1.8 fold more expressed in IR. Seven ATP-binding cassette (ABC) transporter genes were newly identified in A. gossypii, among which only ABCC9 gene was highly expressed in IR. Therefore, this ABCC subfamily, a member of the MRP subfamily which is involved in multi-drug resistance, could be one of the main factors associated with imidacloprid resistance in A. gossypii.

Synthesis of carbon fibers from cotton fiber by pyrolysis process has been described. Synthesis parameters are optimized using Taguchi optimization technique. Synthesized carbon fibers are used for studying hydrogen adsorption capacity using Seivert's apparatus. Transmission electron microscopy analysis and X-ray diffraction of carbon fiber from cotton suggested it to be very transparent type material possessing graphitic nature. Carbon synthesized from cotton fibers under the conditions predicted by Taguchi optimization methodology (no treatment of cotton fiber prior to pyrolysis, temperature of pyrolysis 800℃, Argon as carrier gas and paralyzing time for 2 h) exhibited 7.32 wt% hydrogen adsorption capacity.

Biological control of Aphis gossypii by natural enemies was carried out in watermelon from April to June. Aphidius colemani was released 3 times at rate of 2/㎡ to control A. gossypii(April 24, May 8, and May 22 in 2007). In A. colemani-released plots, A. gossypii showed low population density of ＜ 0.6 per leaf with the highest parasitism of 57.2%, and 2.0~10.6% in the percentage of leaves with aphids. In control, the population of A. gossypii kept on increasing from early May and reached 653.2/leaf on may 29.

The cotton aphid, Aphis gossypii (Glover), is one of the most serious pest in seed potato and various vegetable cultivation. The imidacloprid resistant strain (IR) was over 200 fold more resistant to imidacloprid compared to the susceptible strain (S) as judged by the LC50 values and IR showed cross resistant to acetamiprid, thiamethoxam, thiacloprid, clothianidin. By using the suppression subtractive hybridization method, a imidacloprid resistant associated cDNA library was constructed in adult cotton aphid. In total 115 differentially expressed cDNA clones were obtained. Any point mutation detected in nicotinic acetylcholine receptor alpha 1~5 and beta 1 subunits in the IR. Based on IEF, the IR general esterase isozyme banding patterns were identical with that of S.

Four activated carbons were produced by two-stage process as followings; semi-carbonization of indigenous biomass waste, i.e. cotton stalks, followed by chemical activation with KOH under various activation temperatures and chemical ratios of KOH to semi-carbonized cotton stalks (CCS). The surface area, total pore volume and average pore diameter were evaluated by N2-adsorption at 77 K. The surface morphology and oxygen functional groups were determined by SEM and FTIR, respectively. Batch equilibrium and kinetic studies were carried out by using a basic dye, methylene blue as a probe molecule to evaluate the adsorption capacity and mechanism over the produced carbons. The obtained activated carbon (CCS-1K800) exhibited highly microporous structure with high surface area of 950 m2/g, total pore volume of 0.423 cm3/g and average pore diameter of 17.8 a. The isotherm data fitted well to the Langmuir isotherm with monolayer adsorption capacity of 222 mg/g for CCS-1K800. The kinetic data obtained at different concentrations were analyzed using a pseudo-first-order, pseudo-second-order and intraparticle diffusion equations. The pseudo-second-order model fitted better for kinetic removal of MB dye. The results indicate that such laboratory carbons could be employed as low cost alternative to commercial carbons in wastewater treatment.

In this study, waste natural fibers and natural adhesions were used to prepare an environmental biodegradable nonwoven type mulching material. Especially, the nonwoven mulching with higher content of cotton shows a improved biodegradation. Also, the physical properties of nonwoven mulching with different ratios of cotton/rayon were studied. It was found that the degree of biodegradation was controlled by the type of nonwoven materials and adhesions.

The external ultrastructural morphology and distribution of antennal sensilla and hair-pencils of Palpita indica (Saunder) were examined by scanning electron microscopy. The antennal flagellum of male and female has six types of sensilla: long trichodea, short trichodea, chaetica, coeloconicum around spines, camaniformia and styloconica. There are sexual differences in antennae of P. indica. The female antenna has a coeloconicum sensillum without spines nearby the long trichodea sensilla on ninth flagellomere. Otherwise, auricillica sensilla are distribute on the basal segment of male antenna. These structures are not showed in the female antenna. There are a lot of pores on the hair tufts of the male hair-pencils.

본 연구는 직물구조인자인 위사밀도와 직물의 조직을 변화시켜 시직한 면직물을 이용하여 역학특성 및 태의 변화를 KES-FB System을 이용하여 측정하였다. 그 결과 위사밀도가 증가할수록 굽힘특성 및 전단특성이 증가하는 것으로 나타났고, 위사밀도의 변화에 따른 역학특성치 중 굽힘 및 전단특성의 값은 평직이 높게 나타났으며, 인장특성, 압축특성 그리고 표면특성의 값은 조직 및 밀도에 크게 영향을 받지 않는 것으로 나타났다. 한편, 위사밀도 변화에 따른 의복착용성능 중 WC/T, MMD/SMD를 제외한 B/W 및 2HG/G, 2HB/B, 2HB/W, W/T, 그리고 WC/W는 위사밀도에 영향을 받는 것으로 나타났다. 위사방향의 직축은 굽힘특성, 전단특성, 압축회복도(RC), 표면거칠기(SMD), 태와 의복착용성능에 높은 상관을 보이는 것으로 나타났다. 그리고 Tightness는 굽힘특성, 전단특성, 압축특성 그리고 표면 마찰계수와 높은 상관을 보였고, 기본태 및 T.H.V., 의복착용성능에도 높은 상관이 있는 것으로 나타났다.

톱밥:비트펄프:면실박(50:30:20) 배지의 면실박 대체배지로서 아주까리박, 유채박을 사용한 경우 C/N율은 대조와 유사하였고, 야자박, 코코넛박을 이용한 배지에서는 대조보다 높은 C/N율을 나타났다. 아주까리박, 유채박 사용시 배양일수는 2∼5일, 초발이소요일수는 2일 지연 되었고 수량 또한 대조보다 20g이상 낮게 나타났으며 야자박, 코코넛박 사용시 배양일수는 3일 지연되었으나 수량은 관행재배 128g/병과 비슷하게 나타났다. 경제성 분석결과, 미송톱밥:비트펄프:면실박(50:30:20)의 관행재배에서 면실박 대체배지로 야자박, 코코넛박을 이용시 1000병규모로 연간 8.2회 재배 경우 소득이 각각 2,864천원, 2,733천원으로 관행재배의 2,849천원 대비 101%, 96%의 소득지수를 보였다.