Probiotics are live microorganisms that confer health benefits onto the host when administered at adequate doses. Most widely used probiotics, such as lactobacilli and bifidobacteria, are known to be elements of healthy gut microflora and hence are not considered a threat to the host. However, probiotics may pose a risk in certain populations with compromised immune systems or defects in gut barrier functions. Herein, we evaluated the safety of Bifidobacterium breve BB077, according to the safety evaluation guidelines for probiotics produced by the National Institute of Food and Drug Safety Evaluation (NIFDS). The results show that B. breve BB077 is both non-hemolytic and non-cytolytic. In contrast, B. breve BB077 exhibited higher streptomycin and tetracycline resistance than the suggested NIFDS standard cut-off values. Hence, a genetic analysis of the streptomycin and tetracycline resistance genes was performed to determine the origin of antimicrobial resistance. Streptomycin and tetracycline resistance was shown have arisen from chromosomal mutations and considered intrinsic to the taxonomic group. In conclusion, the B. breve BB077 strain might be safe for human consumption.

In this study, we collect water control valves that have had accidents due to existing cracks, etc. are collected, and propose investigation items for strengthening the valve structural safety evaluation through a series of analyzes from valve specifications to physicochemical properties are proposed. The results of this study are as follows. First, there was a large variation in the thickness of the body or flange of the valves to be investigated, which is considered to be very important factor, because it may affect the safety of the valve body against internal pressure and the flange connected with the bolt nut. Second, 60% of the valves under investigation had many voids in the valve body and flange, etc. and the decrease in thickness due to corrosion was relatively large on the inner surface in contact with water rather than the outer surface. It is judged that the investigation of depth included voids is very important factor. Third, all valves to be investigated are made of gray cast iron foam, and therefore it is judged that there is no major problem in chemical composition. It is judged that the chemical composition should be investigated. Fourth, as a physical investigation item, the analysis of metal morphology structure seems to be a very important factor for nodular cast iron from rather than a gray cast iron foam water valve with a flake structure. As it was found to be 46.7~68.8% of the standard recommended by KS, it could have a direct effect on damage such as cracks, and therefore it is judged that the evaluation of tensile strength is very important in evaluating the safety of the valve.

The development of technology related to the Fourth Industrial Revolution and the growth of the online market due to pandemic are continuing the growth of the logistics market for product delivery. If it is difficult to deliver the product directly to the customer during delivery, storage and delivery using the unmanned courier box are being carried out. However, existing storage boxes are not actively used due to lack of usability even though they have the advantage of storing goods and delivering non-face-to-face. In addition, existing courier boxes are not prepared for cold chain transportation. The unmanned delivery storage device with ICT cold chain technology should be developed to prepare for the transition to non-face-to-face society, to improve logistics efficiency and meet user's requirements. Also, it is necessary to consider the measures to reduce the safety problems that may occur during the use and maintenance of the automatic system.This study conducted a model-based analysis for the development of unmanned delivery storage devices with ICT cold chain technology, and conducted a study to derive the system development specifications that meet the requirements and secure safety and apply them to the development process.

인천광역시에서 유통 중인 농산물을 대상으로 2019년부 터 2021년까지 최근 3년간 잔류농약 모니터링을 실시한 결과 총 7,069건 중 83건(1.2%)이 잔류허용기준을 초과하 였다. 농약 잔류허용기준을 초과한 부적합 농산물의 비율 은 1.0%, 1.4%, 1.1%으로 매년 유사한 수준으로 나타났으 며 잔류농약 검출율은 8.2%, 8.4%, 7.4%를 보였다. 최근 3 년간 diazinon, flubendiamide, procymidone, fluxametamide, fluquinconazole, hexaconazole 등의 순서로 부적합 검출빈 도가 높았으며 농산물 분류별로는 전체 농산물 중 엽채류 와 허브류에서 매년 83% 이상 발생하였다. 부적합 발생 빈도가 높았던 농산물은 고수(잎), 참나물, 취나물, 파 등 이었으며 고수(잎)은 3년 연속으로 부적합 건수가 가장 많 았다. 농산물 생산지역별로는 경기, 인천, 충남, 전남 순으 로 부적합 건수가 많았으나 지역별 출하 농산물의 검사건 수 대비 부적합율은 1.5% 내외로 큰 차이는 없었다. 일부 농산물에서 농약 잔류허용기준의 최대 250배까지 잔류농 약이 검출되는 사례도 발생하므로 부적합이 자주 발생하 는 농산물, 부적합 검출빈도가 높은 농약 성분들의 철저 한 관리가 요구된다. 특히, 농약 사용량 및 사용법 준수, 출하시기 조절 등 농민들의 교육 및 인식 개선이 필요할 것으로 보인다. 안전이 확보된 농산물의 생산과 유통, 소 비자의 안전한 식생활을 보장하기 위해서는 농산물에 대 한 잔류농약 모니터링을 지속적으로 실시하여 부적합 발 생 농산물의 유통을 사전에 차단하는 것이 무엇보다 중요 할 것으로 판단된다.

우리나라는 연안해역을 집약적으로 활용하기 때문에 폐기물로 인한 해양사고 발생률도 높은 편이다. 항해하는 선박의 추진기 에 해양부유물, 폐로프, 폐어망 등이 감기는 사고를 부유물감김사고로 정의하고 있다. 이러한 사고를 예방하기 위해 본 연구에서는 국내 에 상용화되어 세이버 타입(Shaver type)의 로프절단장치를 유한요소법을 이용하여 구조안전성 검토와 수조시험을 통해 절단 과정 및 성능 평가를 진행하였다. 그 결과 로프절단장치를 구성하는 모든 파트는 0.5s 도달하기 전 파손되었으며, 인장강도 대비 발생한 최대응력을 기 준으로 각 파트의 안전계수는 최소 2이상으로 나타났다. 수조시험에서는 세이버타입의 절단장치의 절단 과정을 살펴보았고, 실제 해상에 서 부유중인 폐로프가 다양한 각도로 진입하는 것을 고려하여 케이스 별로 설치 각도를 설정하였다. 그 결과 모든 케이스에서 절단이 되 었으며, 절단 날이 어떠한 각도에 장착되어도 로프를 절단하는데 문제가 없을 것으로 판단된다.

This study evaluated the migrant and residue tests of lead (Pb), cadmium (Cd), nickel (Ni), arsenic (As), and antimony (Sb) in 70 tumbler samples. The migration levels of hazardous metals in all the samples were within the migration limits outlined in the Korean standards and specifications for utensils, containers, and packages. Moreover, in all the tumbler samples, only Ni was detected in 0.5% citric acid solution of a food stimulant. The maximum level of Ni 0.0144 mg/L was 14.4% of the migrant specification (not more than 0.1 mg/L), which was relatively safe. The 0.5% citric acid solution was eluted at 4oC, 70oC, and 100oC for 30 min, and only Ni was detected while testing for migration levels according to the temperature variations; all temperature conditions conformed to the standards. The level of Ni migration increased significantly with increasing migration temperature. Regarding the residue level outside the paint-coated tumbler samples, the Pb level was found to range from N.D. to 20638.1323 mg/kg. The risk of Ni was further estimated to be at a safe level of 0.00 to 0.01% compared to the %TDI as a result.

The present study investigated effects of microbial additives and silo density on chemical compositions, fermentation indices, and aerobic stability of whole crop rice (WCR) silage. The WCR (“Youngwoo”) was harvested at 49.7% dry matter (DM), and ensiled into 500 kg bale silo with two different compaction pressures at 430 kgf (kilogram-force)/cm2 (LOW) and 760 kgf/cm2 (HIGH) densities. All WCR forage were applied distilled water (CON) or mixed inoculants (Lactobacillus brevis 5M2 and Lactobacillus buchneri 6M1) with 1:1 ratio at 1x105 colony forming unit/g (INO). The concentrations of DM, crude protein, ether extract, crude ash, neutral detergent fiber, and acid detergent fiber of whole crop rice before ensiling were 49.7, 9.59, 2.85, 6.74, 39.7, and 21.9%, respectively. Microbial additives and silo density did not affect the chemical compositions of WCR silage (p>0.05). The INO silages had lower lactate (p<0.001), but higher propionate (p<0.001). The LOW silages had higher lactate (p=0.004). The INO silages had higher yeast count (p<0.001) and aerobic stability (p<0.001). However, microbial counts and aerobic stability were not affected by silo density. Therefore, this study concluded that fermentation quality of WCR silage improved by microbial additives, but no effects by silo density.

Probiotic strain is known to regulate the immune system by colonizing in the intestine and interact with intestinal cell receptors of lymphoid tissue. In this study, safety of Streptococcus thermophilus KCTC14471BP and silk fibroin coating effects was evaluated with respect to mucin binding abilities and immune system modulation. S. thermophilus KCTC14471BP was coated with silk fibroin by adding 1% water-soluble calcium and 0.1% silk fibroin. S. thermophilus KCTC14471BP showed the high activities of leucine arylamidase and β-galactosidase. Regarding the antibiotic resistance tests, S. thermophilus KCTC14471BP was susceptible to ampicillin, vancomycin, gentamicin, kanamycin, streptomycin, erythromycin, clindamycin, tetracycline, and chloramphenicol. S. thermophilus KCTC14471BP coated with silk fibroin showed the improved mucin binding ability from 16.1% to 71.3% and was confirmed to have no cytotoxicity against RAW 264.7 macrophage. S. thermophilus KCTC14471BP coated with silk fibroin showed dose-dependently significant increases in pro-inflammatory cytokines IL-1β, IL-6, and TNF-α. These results suggested that S. thermophilus KCTC14471BP can be expected as a promising probiotic bacteria for applications to food industries such as fermentation or functional foods.

The detection of the genome-based antibiotic resistance gene is an essential analysis process for the purpose of verifying the safety of probiotic strains, including lactic acid bacteria. In this study, 4 analysis platforms (AMRFinderPlus, staramr, rgi, ABRicate) were used for cross-comparison of 782 genomes corresponding to 19 kinds of probiotic species notified as functional foods. As a result of the analysis, the relatively fewest number of antibiotic resistance genes were detected in strains belonging to the order Lactobacillales, and antibiotic resistance genes were detected in 322 genomes used in the case of 2 types of Enterococcus genus. In addition, the presence and type of antibiotic resistance gene detection showed a lot of difference even for the same genome sequence depending on the database and analysis algorithm used by the analysis platform. These results can be confused in evaluating the potential for transmission of antibiotic resistance genes inherent in specific lactic acid bacteria and predicting potential risks that may occur in the future. Accordingly, it is judged that the antibiotic resistance gene-related analysis criteria need to be established more clearly and specifically in the safety evaluation of probiotic bacteria.

해저 석유와 가스 탐사가 점점 더 깊은 수심으로 진행되고 있으며, 해저 파이프라인은 고압 및 고온 조건에서 작동하는 것이 일반적이다. 온도 및 압력 차이로 인하여 파이프 축 방향 힘이 축적되는 현상이 있다. 이러한 현상은 파이프라인을 구속하는 해저면 효과 때문에 파이프라인은 횡 좌굴이 발생하게 된다. 온도가 증가하는 경우 축 방향의 압축 하중이 가해지며 이 하중이 임계 수준에 도달하면 파이프가 수직방향으로 움직이게 된다. 또는 파이프라인의 구조적 완전성을 위태롭게 할 수 있는 횡 방향 좌굴이 발생하는 상황에서, 작 동 중 파이프라인의 구조적 안전함을 보장하기 위해 파이프라인의 상세 구조 강도평가가 수행되어야 한다. 본 연구에서는 해저면의 마찰 효과 및 재료의 열 수축/팽창을 고려한 비선형 구조해석을 상용 유한요소해석 프로그램인 ANSYS를 활용하여 검토하였으며, 외부충격에 의한 횡 방향 좌굴 안전성을 분석하였다. 본 연구의 결과를 통하여 수치 해석적 단순화된 분석 모델을 통하여 해저면의 효과를 고려한 조 건에서의 실제 파이프라인의 붕괴 조건을 예측할 수 있다.

Since cranes are a kind of complex human-machine systems, it is almost impossible to completely secure safety with current technologies. Therefore, managerial interventions to prevent human errors are needed for safely operating a crane. The Occupational Safety and Health law states that cabin-type crane operators should have crane drivers’ licence and crane-related operators (e.g., pendent-type crane operators, slinging workers) should take a special safety training. However, statistics on industrial accidents showed that fatalities due to crane accidents (185 accidents occurred during 2013~2017) were the highest among hazardous machinery and equipment. To effectively control the crane-related accidents, voices of crane workers need to be analyzed to investigate the current status. This study surveyed perceived causes of crane accidents and status of special safety training for crane workers of 387. The survey revealed that 24.3% of the respondents experienced crane accidents and 31.4% eye-witnessed crane accidents. 79% of the respondents pointed human errors such as improper crane operation and improper slinging as the first cause. Lastly, only 16.7% of the respondents took a professional special safety training; but the rest took lecture-based or incomplete education. The findings of the present study can be applied to improve crane-related policies and special safety training systems.

In order to provide basic data for preparing management standards and to verify the safety of the Chinese oak mushroom-derived moisturizing medium—which is synthesized and imported in large quantities—the presence of 321 residual pesticides, 7 heavy metals, and 3 radioactive materials was analyzed in the moisturizer samples. Examination of residual pesticides in seven moisturizing medium samples prepared using the Chinese oak mushroom and three domestic sawdust samples used for mushroom culture revealed the presence of cypermethrin and iprodione in three moisturizer samples, but the contents of these pesticides were below the standard limits. Zn was detected in ten samples, Cu was detected in nine samples, and Ni was detected in four samples, but their contents were below the standard limits. Pb, Cd, Cr, and Hg were not detected in any sample. No radioactive materials were detected in the samples. In addition, fruiting bodies of the oak mushroom were observed in each medium. Examination did not reveal the presence of any residual pesticides or harmful compounds. In this study, the use of the moisturizing medium prepared using the Chinese oak mushroom was found to be safe. As residual pesticides, heavy metals, and radioactivity—even in trace amounts—remain concentrated in the human body, continuous verification of the safety of hazardous substances and pollutants during the systematic cultivation and management of these mushrooms is required.