Spotted wing drosophila (SWD), Drosophila suzukii (Diptera: Drosophilidae), is an important quarantine insect pest on blueberry and strawberry exportation in Korea. Due to IPPC recommendations of replacement of methyl bromide (MB) and recent critical issue about its chronic inhalation toxicity to fumigator and related workers along with post-fumigation, alternatives to MB is urgently required. Thus, we evaluated efficacies of ethyl formate (EF) to SWD eggs using two different bioassay methods; using eggs oviposited on artificial diet and on blueberries naturally infested with SWD adults. In results, there was no differences between two bioassay methods in terms of LCT99 value of EF.

Methyl bromide (MB) has been still routinely used in quarantine fumigation on imported citrus, although there had been issuing chronic inhalation toxicity to fumigators and related workers as well as phytotoxic damages after fumigation. Ethyl formate (EF), is the only option to replace MB in terms of its safety for consumers (food additive and naturally occurred) and worker with higher threshold level limit (TLV = 100 ppm). Its application technology also provide cost effectiveness, good commercial practice in terms of application time (< 10 min) for 40 ft container. The replacement of MB with EF is recommended not to fumigate with hazardous and phasing-out MB on imported oranges.

In the 21st century, the risk of the exotic pest being invaded is increasing due to the rise of trading activities. Oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae), has been considered to very destructive invader as it is highly intrusive and has a wide host range. In this study, 2,299 samples were collected from 88 locations in 12 countries by quarantine and investigation. Among them, 608 individuals (including B. correcta) were used for COI DNA barcoding analysis based on Neighbor Joining method with P-distance model. Population genetics analysis was conducted for 510 individuals selected from 47 locations of 12 Southeast Asian countries using 15 microsatellite loci. The barcoding analysis resulted in that any clade was not clustered according to a geographical isolation but indicated geographically mixed populations. Population genetics analysis showed shared genetic structure between neighboring countries across borders. Genetic structure of most Korean quarantine groups was more similar to that of Taiwan, China and Thailand in the order of appearance than other countries.

Origin of the red imported fire ant (RIFA, Solenopsis invicta) is South America, a tropical climate region. It has settled in the invasive area, causing various problems. In recent, colonies of Solenopsis invicta were discovered in Busan in 2017, and then in Busan, Pyongtaek, Incheon, Daegu and Ansan in 2018. This study aims to validate 31 microsatlite loci referred from previous study for tracing origin of Solenopsis invicta colonies. We tried to test previously developed microsatellite loci so that we establish the protocol for molecular epidemiology of RIFA. We applied 31 microsatellite loci to DNA samples from the RIFA colonies found in Texas and Florida, USA. We will present 31 markers were divided into three injections and the amplification rate for each marker was shown as follows.

The red imported fire ant (RIFA, Solenopsis invicta Buren) is defined as invasive species and one of 100 most threaten alien species in world by IUCN. In Korea, RIFA has been first detected at September 28th, 2017 in Busan Port during surveillance by Animal and Plant Quarantine Agency (APQA). The RIFA colony was revealed as a polygyne and H5 type through epidemiological analysis on Gp-9 gene and mt-DNA haplotype. APQA has developed eradication program for RIFA with enhanced surveillance in all nationwide Ports and Internal Container Depots. There are subsequently 7 times more invasions of RIFA in Ports (Busan, Pyongeaek, and Incheon) including 2 times of inlands (Daegu and Ansan) and completely eradicated so far. International trade and climate change increases possibility of establishment and spreading of RIFA in Korea. Therefore, it must be required an international cooperation for prevention of global spreading of RIFA between countries.

해외 수입농산물의 양이 점차적으로 증가하여 다양한 병해충의 유입 가능성이 증가함에 따라, 이들을 신속하고 정확하게 판별하기 위하여 DNA 염기서열을 이용하여 형태학적 동정을 보완하고 있다. 현재 바구미과의 Sternochetus속 에 속하는 관리해충은 S. mangiferae와 S. frigidus, 그리고 S. olivieri가 있으며, 태국에서 수입된 망고에서 2018년 9월부터 2019년 1월까지 7차례에 걸쳐 바구미류가 검출되었고, 형태학적 형질 및 DNA 바코드를 이용하여 S. olivieri로 동정하였다. 추가로, Sternochetus속에 속하는 종들의 분류학적 형질을 이용하여 동정의 수단을 제공하였다. 본 연구결 과는 수입 및 반입되는 망고에서 검출되는 바구미류의 신속하고 정확한 진단에 활용될 수 있을 것으로 판단된다.

We conducted quarantine insect species diversity monitoring using DNA barcoding with 517 lepidopteran samples that were obtained from quarantine inspections of foreign vessels entering Korea. The DNA barcode of each sample was treated as a molecular operational taxonomic unit (MOTU). For species delimitation and species identification of the analyzed samples, we applied a 2% cutoff rule and then identified with using BLAST of NCBI GenBank and BOLD System ver. 3.0. Consequently, 517 analyzed samples were delimited as 214 putative species across 20 families. Of these 214 putative species 145 (368 samples) were considered taxonomically identified if the closest BLAST match was no more than 2% different. Therefore the number of samples that were identified to the species level was relatively low, at approximately 71%. 115 of the 145 species were known in Korea. Of the 30 species that were not known in Korea, three, i.e., Noctua pronuba (Noctuidae), Orthosia hibisci (Noctuidae), and Pieris brassicae (Pieridae), were checked as ‘Regulated pests’ in Korea. We suggest that the three regulated pest species could be prevented from being introduced to Korea if monitoring of the vessels that pass the navigation route that contains these three species is performed consistently. Therefore, we suggest that the monitoring of quarantined insect pest enables the prevention of the introduction of alien species.

Whitefly (Hemiptera: Aleyrodidae) pests, including the tobacco whitefly, Bemisia tabaci, and the greenhouse whitefly, Trialeurodes vaporariorum, are economically important in agriculture. With the annual growth of the domestic fresh fruit export market, various quarantine treatment methods are being used to export strawberries of better quality. The objective of the present study was to evaluate the effects of gamma rays on the development and reproductive sterility of B. tabaci and T. vaporariorum. In both species, the eggs were completely inhibited from hatching at 50 Gy, and the emergence of 3rd instar nymphs was completely suppressed at 150 Gy. Some adult B. tabaci and T. vaporariorum spawning occurred at 100 Gy and 70 Gy, respectively; however, at these irradiation levels, F1 hatchability was completely inhibited. Dosimetry results showed that the penetrating power of gamma ray in the strawberry-filled box was the lowest at the mid-box position. Therefore, B. tabaci and T. vaporariorum were placed in the middle of the strawberry-filled box and irradiated. A gamma-ray irradiation of 100 Gy suppressed the development and reproduction of eggs and adults in both B. tabaci and T. vaporariorum. However, the development of 3rd instar nymphs was inhibited at 300 Gy for B. tabaci and 200 Gy for T. vaporariorum. Our data suggest that at least 300 Gy should be used for the control of these two types of whitefly for strawberry export.

This study was done to develop environmental risk assessments and a biosafety guide for insect-resistant genetically modified rice at a LMO (Living Modified Organism) isolation field. In the LMO quarantine area of Kyungpook National University, the species diversities and population densities of non-target insects found on insect-resistant genetically modified rice (Bt-T) resistant to Cnaphalocrocis medinalis and on non-GM rices (Dongjin-byeo and Ilmi-byeo) were investigated. The Bt-T event was therefore evaluated under field conditions to detect possible impacts on the above ground insects and spiders. The study compared transgenic rice and two non-GM reference rices, Dongjinbyeo and Ilmi-byeo, at Gunwi in Southern Korea in 2016 and 2017. A total of 9,552 individuals from 51 families and 11 orders were collected from the LMO isolation field. In the three types of rice fields, a total of 3,042 individuals from the Bt-T, 3,213 individuals from the Dongjin-byeo, and 3,297 individuals from the Ilmi-byeo were collected, respectively. There was no difference between the population densities of the non-target insect pests, natural enemies and other insects on the Bt-T and non-GM rices. The data of insect species population densities were subjected to principal component analysis (PCA), which not distinguished among the three varieties, GM and non-GM, reference cultivar in all cultivation years. However, PCA results were clearly separated the samples based on the cultivation years. These results suggest that insect species diversities and population densities during plants cultivation are determined by environmental factors (growing condition and seasons) rather than by genetic factors.

국제교역의 발달로 외래병해충의 국내 침입 가능성이 높아지고 있으며, 기후 변화로 인해 토착화 가능성 또한 점차 증가하고 있다. 이에 대한 선제적인 연구를 위해 기존의 생물안전 연구 시설을 바탕으로 검역해충 실험동을 농림축산검역본부에 구축하였다. 본 시설은 검역해충에 대한 전문연구시설로써 내부해충의 외부유출을 방지하기 위해 실험실 입실시 풍속 25-35m/s 의 에어샤워 부스 통과시설과 각 연구실 출입문에 풍속 9-12m/s 의 샤워커튼이 설치되어 있으며, 자외선 해충유인등이 실험실 내외부 벽면에 장착되었다. 에어샤워 부스와 샤워커튼은 호박꽃과실파리. 벗초파리의 비행에 영향을 주어 실험실 외부로 유출하는 것을 방지할 수 있었다. 또한 이러한 연구를 바탕으로 실험실 표준운영 절차를 수립할 수 있었다.

나무이는 감귤그린병, 제브라칩병과 같은 식물병원균의 매개충이 되는 경제적으로 중요한 해충그룹으로서, 귤나무이(Diaphorina citri Kuwayama)는 감귤산업에 큰 피해를 주는 감귤그린병(Citrus Huanglongbing Disease)을 매개하여 전 세계적으로 문제가 되고 있다. 최근 기후 온난화로 귤나무이가 유입될시 제주도를 비롯한 남부지방에서도 하우스 재배 감귤에는 충분히 월동 가능할 것으로 분석된다(감귤연구소, 2015).제주도지역이 경우, 묘목 수입에 의하여 전파될 가능성이 가장 클 것으로 예상된다. 이에 국내 유입 대비를 위한 예찰방법 및 동정법 개발이 필요한 실정이다. 제주도 지역을 2016년부터 현재까지 43지점을 조사한 결과, 18종의 나무이와 14종의 기주식물을 확인되었다. 또한 실내사육을 통해서 생육단계별 생태사진과 동정에 필요한 암수 생식기 표본, DNA 염기서열을 확보하였다. 현재까지 운향과를 기주로하는 나무이는 발견되지 않았으며 귤나무이 또한 발견되지 않았다.

기존 발표된 CLIMEX 매개변수와 RCP 8.5 시나리오에서의 기후자료를 이용하여 우리나라 금지급 과실파리 12종의 국내에서의 향후 기후적합도를 산출하고 국내정착가능 여부를 평가하였다. 열대내지 아열대성인 Anastrepha ludens와 A.obliqua, Zeugodacus cucurbitae, Bactrocera latifrons, B.tryoni, Ceratitis capitata, C.rosa는 모두 2010년대에는 제주도 남부지방 또는 해안가 일부가 경계 지역으로써 정착 가능한 것으로 평가되었다. B.correcta와 B.zonata는 2010년대에 제주 산간을 제외한 전역과 남해안 극히 일부 지역이 경계 지역에 포함되며, B.zonata, B.latifrons의 경우 제주에 적절내지 최적 지역이 형성되었다. 중북부, 내륙에서 이들 종의 기후적합성이 부적절한 것은 주로 저온 스트레스에 의한 것으로써 지구 온난화에 의해 기온이 상승하면서 2090년대에는 경계지역 범위가 해안가를 따라 상승하였다. 한편, B.tsuneonis와 Rhagoletis pomonella는 온대성 과실파리로서 주된 스트레스 요인은 습윤 스트레스였다. B.tsuneonis 는 2010년대부터 국내 산간을 제외한 대부분 지역이 이들 종의 최적 지역에 포함되었으며 그 범위는 점점 확장되었다. R.pomonella는 국내 대부분 지역이 적절 지역으로 평가되나 2050년, 2090년대에는 제주도 산간을 중심으로 정착 부절절 지역의 면적이 확장되었다. 한편, R.indifferens는 국내 중부지역을 중심으로 한 중산간 지역이 경계내지 적절 기후로 평가되나 정착가능 면적은 점차로 좁아져 2090년대에는 산간 극히 일부지역만 경계 지역으로 포함되었다.

2018년 현재까지 농림축산검역본부 호남지역본부로 수입된 식물은 220종 17,030건으로 우드펠렛(18%), 주정박(8%), 페스큐 건초(8%), 알파파건초(8%), 귀리건초(6%), 혼합건초(5%), 티모시건초(5%), 라이그래스건초(5%), 목화씨(사료용)(4%), 소나무원목(3%) 등의 순이다. 이와 같이 호남지역은 비재식 사료류(건초 등) 수입비중이 높으며 이는 전국 수입 건수의 약 67%에 달한다. 검역해충은 795건 검출되었으며 이 중 관리해충 294건, 잠정규제해충 146건, 비검역해충 307건 등이다. 관리해충 중 Carpophilus obsoletus가 210건(72%), 잠정규제해충 중 Cryptoletes ferrugineus가 56건(39%)으로 높은 검출율을 보였다. 또한 호남지역으로 수입 된 재식용 구근류는 생강 및 생강구근, 마늘구근이며 전국 수입 건수의 약 42%를 차지한다. 수입 된 생강 및 생강구근 91건 중 37건에서 해충(선충)을 발견하였고 Meloidogyne sp.(잠정규제해충) 검출로 9건이 폐기·반송되었다. 수입된 마늘구근 34건 중 29건에서 해충을 발견하였고 Carpophilus obsoletus(관리해충) 18건, Cryptoletes ferrugineus(잠정규제해충) 11건이 검출되어 소독하였다.

농림축산검역본부에서는 수입식물 검역과정, 현지조사, 연구사업 등을 통해 다양한 병해충 표본들이 확보되고 있으며, 2016년도부터 병해충 표본자원의 DB구축사업을 실시하고 있다. 농림축산검역본부의 해충표본실에는 2018년도 9월까지 약 6,400점의 표본 등록 및 바코드화가 완료되었으며, 올해까지 총 7,000점의 표본이 등록될 것으로 예상된다. 그 중 노린재목(Hemiptera)이 5,500점으로 전체의 85%를 차지하고 딱정벌레목(Coleoptera)이 377점으로 6%, 파리목(Diptera)이 188점으로 3%를 차지한다. 국제적으로 생물자원의 중요성과 확보 경쟁이 치열한 상황에서 생물표본가치의 증가 및 자료 구축을 통해 앞으로의 첨단 동정기술 개발과 동정매뉴얼 제작 및 업데이트를 위한 필수적인 재료로써 활용될 것으로 기대된다.

Here, we provided a lucid key for twelve lepidopteran quarantine pests in South Korea. They were selected based on border quarantine inspection data from 1996 to 2016 in S. Korea. They are belonging to eight families: Carposinidae, Crambidae, Pyralidae, Gelechiidae, Oecophoridae, Tineidae, Tortricidae and Geometridae, and also categorized into the three groups: prohibited, regulated and nonquarantine pests. The key includes diagnostic characters from head, thorax, fore- and hind-wings for each species examined.

지난 19년간(2000~2018.9) 검역현장에서 검출된 해충의 종류는 2,028종이며 150,379회 이다. 이중 곤충은 1,745종으로 86%이며, 딱정벌레목은 검출종 중 740종, 42,4%로 검역적으로 매우 중요하다. 밑빠진벌레과(Nitidulidae)는 딱정벌레목 머리대장상과에 포함되고, 검출율은 9.0%이다. 검출율이 가장 높은 종은 Brachypeplus rubidus로 1,415회 검출되었으며, Carpophilus obsoletus는 999회, Carpophilus hemipterus는 927회, Carpophilus dimidiatus는 477회 검출되었다. 본 연구는 검역과정에서 검출되는 밑빠진벌레과의 Carpophilus 속에 속하는 종들에 대해 검토 하였다. 또한 이들의 외형적 특징과 종의 분류 검색을 제시 하고자 한다.

지난 19년간(2000~2018.9) 검역현장에서 검출된 해충의 종류는 2,028종이며 150,379회 이다. 이중 곤충은 1,745종으로 86%이며, 딱정벌레목은 검출종 중 740종, 42,4%로 검역적으로 매우 중요하다. 머리대장과(Cucujidae)는 머리대장상과에 포함되고, 검출율은 14.7%로 상위 두 번째 과에 속한다. 특히 Cryptolestes ferrugineus(갈색머리대장), Cryptolestes turcicus(터키모진가슴머리대장), Cryptolestes pusillus(긴수염머리대장)은 딱정벌레목 검출종 중 상위 10위 안에 속해 있는 종으로 검역현장에서 매우 중요시 되고 있는 종이다. 본 연구는 머리대장과 C. ferrugineus(잠정규제), C. turcicus(잠정규제), C. pusillus(비검역), C.pusilloides(관리급)에 대한 외형적 특성과 특징을 소개하고자 한다.

The overall goal of this USDA-ARS research is to ensure the protection and quality of stored product foodstuffs. The results of this research directly enhance production, distribution, and safety of foodstuffs, promote and retain access of United States-grown crops to domestic and foreign markets, and protect the United States and trading partners from the agricultural, ecological, and economic threat posed by quarantine and invasive pests. In general, USDA-ARS research related to the fumigation of stored products focuses on the development of techniques to rapidly disinfest raw products of field pests, control pests in processed products amenable to re-infestation and microbial infection, and reduce reliance on fumigation as a stand-alone measure for postharvest disinfestations and disinfections. Specific research objectives include: comparative evaluation of alternative fumigants to methyl bromide in postharvest applications, development of novel technologies to reduce and eliminate atmospheric emissions from chambers used in postharvest fumigation, and design production strategies that allow for a more strategic postharvest use of methyl bromide and alternative fumigants. Recent research findings will be presented and discussed.

Methyl bromide (MB) had been routinely used in quarantine and pre-shipment (QPS), despite of strong recommendations to replace technically feasible alternatives of it by International Plant Protection Convention (IPPC) and current hazardous issues in workplace related post-fumigation process of imported perishable commodities. Due to insufficient ventilations post-fumigation on imported fruits such as citrus, fumigators and related workers in cold storage, load/unload and packing room has been exposed to hazardous conditions (5-100 ppm, MB’s TWA_TLV = 1 ppm). It’s time to replace newly developed phytosanitary disinfestation methods such liquid ethyl formate (EF) with N2 application. It could provide for safer workplace involved on and post-fumigation process, cost-effectiveness as well as meet the environmental friendly solution.

Effective phytosanitary fumigation can prevent the introduction of exotic insects into new areas where they become pests. Traditionally Probit 9 level control (99.9968%) was considered as a stand-alone quarantine treatment. However, unacceptable phytotoxic damage often associated with high-dose treatment to realize Probit 9 level control of a large number of pests has often restricted its practical application. Therefore, quarantine security is being achieved for some commodities using a “systems approach”, where quarantine pests are cumulatively reduced to acceptable levels using independent, pre- and postharvest measures that comprise a systems that effectively mitigate the pest. Preharvest reductions in population through the study and application of chemical ecology involving insect behavior modifying chemicals can be an integral part of systems approach. This results in low pest prevalence prior to end point treatment, where pests may be effectively controlled using a less-than-Probit 9 level, low-dose treatment. In this talk, I discuss the chemical ecology of invasive quarantine pests including Drosophila suzukii and its perspective in the effective end point fumigation treatment using ethyl formate.