Interest in the measurement of siloxane which reduces energy efficiency of biogas, has increased with its market extend. Even though the impinger absorption method takes long sampling time of 2-3 hours and need a complicated equipment, it has been typically used for the sampling method of siloxane. This study was conducted to apply the gas bag sampling method with tedlar bag and aluminium bag for improving the method of siloxane sampling. To compare efficiencies of siloxane sampling, the manufactured gas, landfill gas, and digestion gas were used as sample gases. According to the result, materials of gas bags did not cause measurement error and there was no loss of siloxane by adsorption on the inner surface of gas bag. The result of D4 calibration in the tedlar bag, showed higher than 0.99 in the coefficient of determination. In case of digestion gas, the analysis results of two samples collected by the tedlar bag and the impinger absorption were almost same. The differences of analysis result between landfill gas and digestion gas were considered due to the short sampling time and the absence of gas storage tank.

To promote applicability of steel bridge coating diagnosing system developed and used by Korea Expressway Corporation, problems found in deterioration degree evaluation standard for steel bridge coatings were revised in this study. Suitability of revised evaluation standard was confirmed by field survey.

본 연구에서는 주어진 호우사상을 베타분포로 적절히 표현하기 위한 매개변수의 결정 방법을 제시하였다. 이를 위해 첨두에 대한 고려를 추가한 경우와 그렇지 않은 경우, 실제 우량주상도 및 첨두를 중심으로 재배열된 수정 우량주상도를 사용하는 경우 등을 고려하여 그 특성을 비교 검토하였다. 본 연구는 서울 지점의 2010년 독립 호우사상과 1961∼2010년 사이의 연 최대치 독립 호우사상을 대상으로 베타분포를 적용하고, 그 결과를 분석하였다. 그 결과를 정리하면 다음과 같다. 첫째, 베타분포를 이용하여 실제 호우사상의 우량주상도 형태를 유사하게 표현하기 위해서는 첨두에 대한 추가적인 고려가 필요한 것으로 확인되었다. 둘째, 첨두를 중심으로 시간강우를 좌우 순차적으로 재배열한 수정 우량주상도를 이용하는 경우가 실제 우량주상도를 이용하는 경우보다 실제 강우의 시간분포 특성에 보다 가까운 베타분포를 유도함을 확인하였다.

본 연구에서는 소규모 지구내 저류시설에 대한 유출저감효과를 정량화하였으며, 가용지를 대상으로 설계규모의 적정성을 평가하였다. 대상 시설에 대한 유출모의를 위해 수정합리식을 이용하였으며, 유출저감효과는 집수면의 토지피복 특성(유출계수), 강우특성(강우 재현기간, 지속기간), 저류시설의 규모(저류가능면적률, 저류한계수심)별로 정리하였다. 아울러 기성시가지 유역을 대상으로 지구내 저류시설로 활용 가능한 지역을 현장조사를 통해 살펴보았으며, 가용한 지역에 지구내 저류시설 설치시 설계규모의 적정성을 평가하였다. 본 연구의 결과는 기성시가지 유역의 제한된 여건 속에서 지구내 저류시설을 고려할 경우, 예상되는 유출저감효과를 간단히 파악할 수 있을 뿐만 아니라 적정 설계규모를 결정하는데 이용될 수 있을 것이다.

최근 들어 도시화와 이상기후의 영향으로 유역에서의 유출특성과 오염물질 발생특성도 변화하고 있고 이에 따라 정교한 유역 수질분석이 요구되고 있다. 유역의 비점오염원 관리를 위하여 충분조건을 갖추어야 할 정량분석이 선행되어야 하나 점오염원 관리와 달리 자연 내의 복잡한 현상을 반영하여야 하기 때문에 비점오염원의 정량화 해석에 어려움을 겪어왔다. 강우 및 유출량 관계와 덧붙여 오염물질이 하천수질에 영향을 미치기까지 도달시간 해석 및 점오염원과 비점 오염원의

Recent interest in green tea polyphenols has increased owing to their antioxidant activities and their possible role in the prevention of cancer and cardiovascular diseases. Typically, 93% compounds of total polyphenols in green tea leaves, many of which are called catechins, are flavonoids. The major tea catechins are a mixture of epicatechin isomers, including (-)-epigallocatechin gallate (EGCG), (-)-epigallocatechin (EGC), (-)-epicatechin gallate (ECG), and (-)-epicatechin (EC). Separation of green tea catechins is generally carried out by HPLC-UVD. UV detector is a suitable detector for quantification this class of molecules and allows high sensitivity level for polyunsaturated species. However, UV detection does not discriminate different compounds having similar chromophore groups. More detailed structural information can be collected when a mass spectrometer is coupled with a UV-DAD. MSD is a powerful tool for qualitative analysis to identify and confirm molecular structures of unknown compounds, and it is particularly useful for quantitative analysis, owing to its high sensitivity and selectivity. This study describes the application of HPLC-DAD/MS methods for the rapid and routine analysis of 9 catechins in green tea leaves. Green tea extracts were injected directly onto a reversed phase HPLC column. Compounds, identified on the basis of their absorbance and MS spectrum, included gallic acid, (-)-epicatechin, and their various gallate derivatives. HPLC/MS detection was found to be more sensitive than UV-DAD, and MSD showed good precisions for quantification of catechins.

As forest land takes up 65% of the Korean peninsula, there have been continuing conflicts between the development and conservation of forest land. As the income level has changed over time, the usage of forest land in society has changed. There has been increasing demands for forest land for urban development and recreational use. On the other hand, a large proportion of the land is required to be preserved for the forest and the natural ecosystem in it. The existing management system for the forest land has been designed focusing on the management of the trees on the land, and not the land itself. Due to this limitation, the current management system of forest land has failed to protect the forest land from being developed indiscreetly, making it difficult to conserve and develop the forest land in an efficient way. A major question in forest land management is how to integrate economic use activities with the supporting ecosystems to maximize performance of the ecological-economic system. In order to promote sustainable use of forest resources, and to achieve efficient forest land management, it is prerequisite to evaluation on forest resources of natural ecosystems. Quantitative measures are needed that signify how necessary the services and products of forested ecosystems are to human endeavors. In this study, the natural wealth provided by forest land was quantified based on emergy synthesis. Emergy is a universal measure of real wealth of the work of nature and society made on a common basis. Thus, Calculations of emergy provide a basis for making choices about environment and economy following the general public policy to maximize real wealth. The goals of forest land management to achieve balance between the ecology and economy of its integrated system and to foster equity among the diverse outcomes of the forest land were assessed with emergy. Emergy was demonstrated to holistically integrated and quantify the interconnections of a coupled nature-human system allowing the goals of ecological balance and outcome equity to be measured quantitatively. Doing so will provide a better understanding of the basis of forest land wealth and the consequences of management decisions.

폐콘크리트로부터 생산된 순환골재의 사용은 환경보존과 자원의 재활용 관점에서 매우 유용하며, 순환골재 콘크리트에서 압축, 인장, 휨 및 부착강도, 탄성계수 등이 중요한 기계적 특성요소로 작용하게 된다. 특히 압축을 받는 순환골재 콘크리트의 응력-변형률 관계 및 파괴진전 양상 규명은 순환골재 콘크리트를 사용한 구조물 설계 및 수치해석 등 이론적 연구에서도 매우 중요한 의미를 가진다. 따라서 본 연구에서는 순환골재 콘크리트의 파괴진전특성을 규명하기 위하여 압축하중을 받는 콘크리트의 미세균열 등 손상특성을 검출하기 위하여 AE 기법을 사용하였다. 압축거동특성 및 AE 신호특성을 분석한 결과, 순환굵은골재 콘크리트의 균열 및 파괴거동은 천연 및 순환잔골재를 사용한 콘크리트와 상이한 것으로 나타났다.

Mung bean (Vigna radiata L.) is an increasingly important human food source, as well as a new functional agent, mainly due to its potent antioxidant activity. This study was conducted to determine antioxidant activity of fractions from mung bean seeds by measuring DPPH radical scavenging activity and to quantify the flavonoids by means of HPLC analysis. Vitexin and isovitexin were present in both ethanol and water extracts in highest amount. Flavonoids, vitexin and isovitexin were quantified from 195 germplasms of mung beans and their concentrations varied by 4.7 fold. Especially, the breeding line KM99004-4B-2 (Suwon28/KM94004), which has grown in Jeollanamdo Agricultural Research and Extension Services, showed the highest amount (15.88 mg/g) of total flavonoids. The vitexin portion was averaged 70.73±1.38%. High positive correlation (r=0.96***) between vitexin and isovitexin contents showed. However, the flavonoid content showed very low correlation with the 24 growth and ecological characteristics. Seed coats of mung beans had the highest flavonoid amount, showing 50~70 times more than cotyledons. Flavonoid contents in the seed, the cotyledon, and the seed coat were decreased as the seed imbibition time increased.

미강에 함유되어 있는 토코페롤 및 토코트리에놀의 함량을 비파괴적으로 신속하게 추정하기 위하여 NIRS(근적외선 분광분석기)를 이용한 분석 방법을 검토하였다. 벼 유전자원 80계통의 미장을 사용하여 HPLC에서 분석된 토코페롤 및 토코트리에놀의 함량치를 NIRS 스펙트럼에 적용시킨 후 검량식을 작성하였다. NIRS의 검량식을 몇가지 방법에 의하여 비교 분석한 결과 2차 미분된 스펙트럼을 MPLS(Modified Partial Least Squares)를 이용한 회귀식에 이용하는 것이 가장 적합하였다. HPLC를 이용한 유전자원들의 성분 함량과 NIRS에서 도출된 검량식과의 상관계수는 토코페롤과 토코트리에놀이 각각 0.992, 0.953을 나타내었다. 이들 검량식은 validation file 에서도 0.846 및 0.956의 높은 상관을 보여 미강 상태에서 토코페롤 및 토코트리에놀의 함량을 NIRS를 이용하여 신속하게 분석할 수 있을 것으로 판단되었다.

Greenhouse and laboratory studies were conducted to determine the allelopathic potentials of extracts or residues from sweet potato (Ipomoea batatas L. (Lam). The extracts applied on filter paper in a Petri dish bioassay significantly inhibited root growth of alfalfa. Aqueous leachates at 40g dry tissue ~textrmL-1 (g ~textrmL-1 ) from leaves showed the highest inhibition against alfalfa, and followed by stems and roots. Alfalfa root growth was significantly inhibited by methanol extracts of the same plants as the concentration increased. The effect of residue incorporation into soil on seedling growth of com, soybean, barnyard grass and eclipta was examined in the greenhouse, and results showed that the leaf residues at 200g ~textrmkg-1 by plant parts inhibited shoot dry and root dry weights of test plants by 60-80%. By means of HPLC, causative allelopathic substances present in plant parts of sweet potato "Sinyulmi" were identified as coumarin, trans-cinnamic acid, o-coumaric acid, p-coumaric acid, and chlorogenic acid. Total content of these compounds for leaves extracts were detected as the greatest amount in EtOAc fraction, especially trans-cinnamic acid was the greatest component. These results suggest that sweet potato plants have herbicidal potentials, and that their activities exhibit differently depending on plant parts.ant parts.

삼지구엽초에 함유되어 있는 icariin 함량을 신속하게 추정하기 위하여 NIRS(근적외선 분광분석기)를 이용한 분석 방법을 검토하였다. HPLC를 이용하여 분석된 삼지구엽초 유전자원 150계통에 대한 이카린 함량치를 NIRS 스펙트럼에 적용시켜 42개의 calibration set 와 26개의 valilion set를 구분하였다. NIRS의 검량식을 몇가지 방법에 의하여 비교분석한 결과 2차미분된 스텍트럼을 MPLS(Modified Partial Least Squares)를 이용한 회귀식에 이용하는 것이 가장 적합하였다. HPLC를 이용한 유전자원들의 이카린 함량은 평균 0.424%(0.12~0.67%)이었으며, NIRS에서 도출된 검량식과의 상관계수는 0.951을 나타내었다. 따라서 삼지구엽초의 이카린 함량은 NIRS를 이용하여 신속 편리하게 분석할 수 있음이 인정되었다.

Momilactones A and B, the major phytotoxins and phytoalexins in rice plants, were quantified by a HPLC-APCI-MS-MS (APCI-MS-MS) system under multiple reaction monitoring conditions. Since MA and MB were found to be easily extracted with water, these phytotoxic compounds may affect germination and growth of other plant species when the rice straws were left in the fields.

This study was carried out to evaluate the component and content of anthocyanin pigments, free sugars, and organic acids in mulberry fruit. Fructose and glucose were identified as the major free sugars, and oxalic, malonic, succinic, malic, and citric aci