This study was performed observe the effect of vegetable oil on the liver of 0.5% cholesterol-fed rats. In this experiment, male rats of Sprague-Dawley strain were used. The rats were divided into 5groups which were fed differently either for 8 weeks: basal diet, 20% sunflower oil diet, 20% soybean oil diet, 20% rapeseed oil diet.,20% coconut oil diet. The total cholesterol, triglyceride level in the liver were showed tendency of increase with increasing of P/S ratios. Bile acid excretion in the fecal increased with increasing of P/S ratios. The value of TBA in the serum and liver were increased in proportion to the amount of polyunsaturated fatty acid. The liver fatty acid of coconut-fed group showed larger variation than fatty acid of the cocount oil. The fat chang of sunflower oil diet in the liver showed the largest change.

에어로빅 댄스에 의한 혈청지질의 변화, 특히 에어로빅 운동 수행기간에 따른 혈청 HDL-C의 변화를 알아보기 위해 서울에 거주하는 35명의 중년여성(35∼45세)을 대상으로 체위측정 및 혈청지질 성분을 분석하였다. 즉 운동을 하지 않는 좌식생활자 8명을 control군으로 하고 에어로빅 댄스를 하는 자 27명을 2∼3개월 수행자군 (AⅠ군), 4∼10개월 수행주군 (AⅡ군), 1년 이상 수행자군(AⅢ군)으로 나누어 그들의 혈청내 total cholesterol, triglyceride, HDL-cholesterol의 농도를 측정한 바 다음과 같은 결과와 결론을 얻었다. 1. 혈청 TG농도는 에어로빅 수행군이 좌식생활자(C군)에 비해 현저하게 낮게 나타났으며(p<0.001) 에어로빅 수행기간이 길수록 낮아졌지만 유의적인 차이는 없었다. 2. 혈청 TC농도는 에어로빅 수행군이 좌식생활자에 비해 낮게(10%) 나타났으나 통계적으로 유의적인 차이는 없었으며 에어로빅 수행기간에 따른 구룹간의 혈청 TC의 농도도 유의적인 차이가 없었다. 3. 혈청 HDL-C농도는 2∼3개월 에어로빅 수행자가 좌식생활자들에 비해 유의하게 높았으며(p<0.05) 에어로빅 수행기간에 의한 구룹간의 HDL-C농도는 유의하게 차이를 나타냈다(p<0.05). 즉 에어로빅 수행 기간이 길수록 혈청 HDL-C농도가 높은 수치로 나타냈다.

석유 에테르로 인삼지방질 성분을 추출해 낸 소위 탈지인삼을 가지고 기본 식이에 0.5, 1.0 및 3.0% 비율별로 각기 혼합하여 만든 탈지인삼식이로 이유 후 체중 83±4g 웅성백서 130두를 대상으로 4주 또는 8주동안 급식한 후 기본식이로 교체하여 다시 8주 또는 4주동안 급식하여, 교체급식에 의한 흰쥐의 혈청, 간 및 대동맥중의 콜레스테롤함량 변화의 추이를 비교 관찰한 바 다음과 같은 결과를 얻었다. 1. 혈청중 총 콜레스테롤과 유리콜레스테롤 함량은 0.5, 1.0 및 3.0% 탈지인삼식이 급식후 기본식이로 교체하여 급식시 증가되었다. 2. 대동맥중 총 콜레스테롤 함량은 0.5, 1.0 및 3.0% 탈지인삼식이 급식후 기본 식이로 교체하여 급식시 감소되었다. 3. 혈청, 간 및 대동맥 중 유리콜레스테롤 함량은 1.0% 탈지안심식이 급식후 기본식이로 교체하여 급식시 증가되었으나, 혈청과 간중 유리콜레스테롤 함량은 3.0%탈지 인삼식이의 경우 감소되었다.

To study effects of wild garlic on components of lipid and protein, blood glucose level, and cholinesterase activity in rat's serum, Sprague-Dawley strain of 40 male rats are divided into 5 groups which are Normal, Control, A, B, and C group. Normal group is fed only Basal diet, and Control group is fed Basal diet and 0.5ml/day of 2.5% cholesterol solution, In addition to a diet of control group, A group is fed 25% wild garlic flour, B group 0.5ml/2day of concentratded wild garlic juice, C group 0.5ml/2day of concentrated ethanol extract of wild garlic, After 8 weesk the rats were fasted for 12 hours, and then decapitated to collect blood. The results of analysis of the rat's serum were summarized as follows, 1. Wild garlic diet, specially ethanol extract has an influence on decreasing the level of total cholesterol and blood glucose in rat's serum, 2. HDL-cholesterol and phospholipid content of rat's serum are increased by wild garlic diet. Therefore I think that wild garlic is good food for preventing development of atherosclerosis and diabetes.

This study was performed observe the effect of edible oil on the serum lipid of 0.5% cholesterol-fed rats. In this experiment, male rats of Sprague-Dawley strain were used. The rats were divided into 5groups which were fed differently eigher for 8 weeks : basal diet, 20% sunflower oil diet, 20% soybean oil diet, 20% rapeseed oil diet, 20% coconut oil diet. The followings are the results of this experiment. 1. The total chol., free chol., TG, PL level in the serum were showed tendency of decrease with in creasing of P/S ratios. 2. HDL-chol. level was increased with increasing of P/S ratios but LDL-chol. level was decreased. 3. GOT, GPT, TBA level in the serum were increased with increasing of P/S ratios.

This study aimed to find out the effect of freeze-drying leek against cholesterol feeding rats on the cholesterol and lipid in serum, hemolysis in erythrocyte and blood pressure in rats tall. In this experiment, male rats of Wistar strain were used. The rats were divided into 3 groups in 8 weeks : basal diet, 1% cholesterol and 1% cholesterol and 1% leek. The followings are the results of this experiment. 1. In vitro, leek-ext, reduced the hemolysis. 2. Leek reduced VLDL, LDL/HDL-cholesterol in serum. 3. Leek reduced the blood pressure in rat's tail. 4. Leek reduced the fatty change in liver caused by cholesterol treatment. This experiment showed that leek-addition group had portective effect against cholesterol fed and decresed VLDL, LDL/HDL-cholesterol in serum, Leek alleviated hemolysis in erythrocyte, blood pressure in rat's tail and fatty change in liver. Therefore, this experiment concluded that leek has defensive power against cholesterol.

The study was accomplished to know the effects of garlic on body weight, and serum lipid, protein and glucose in male rats. The rats applied in this study were Sprague-Dawley strain of 42. In addition to basal diet, they were fed together raw garlic juice or ethanol garlic extract with 2.5% cholesterol solution solved by corn oil for 8 weeks respectively. These results were as follows. 1. The growth rate and efficiency ratio appeared to be more increased in ethanol garlic extract groups than in raw garlic juice groups. 2. The content of serum total cholesterol appeared to be the most decreased in 0.4ml ethanol garlic extract group. 3. The level of serum HDL-cholesterol had a tendency to be increased in all garlic groups. 4. Albumin/Globulin ratio appeared to be more decreased in raw garlic juice groups than in ethanol gar lie extract groups. 5. The level of serum glucose appeared to be more decreased in ethanol garlic extract groups than in raw garlic juice groups. Therefore we think that garlic is able to have an effect on atherosclerosis and diabetes.

Effect of perilla oil on the fatty acid composition, ACAT and HMG-CoA reductase in the liver microsomes, or cholesterol and protein in serum of rabbit were examined. 1. The content of total protein in serum was almost same amount of both groups, but α1-globulin and r-globuline were incresed or β-globulin was decresed compared with control. 2. The content of high density lipoprotein incresed, and the content of low density lipoprotein decresed in lipoprotein. 3. Total cholesterol and triglyceride were decresed, and the content of phospholipid was incresed. 4. Perilla oil did not effect for changing blood glucose and Na+, K+ electrolytes. 5. Perilla oil did not effect for changing serum GOT and GPT in rabbit. 6. The activity of ACAT decresed and the activity of HMG-CoA reductase incresed. The activity of ACAT and HMG-CoA reductase in liver microsomes were reciprocal. 7. There were arachidonic acid 20:4, eicosapentaenoic acid 20:5, and docosahexaenoic acid 22:6 in the liver microsomes of rabbits. These highly polyunsaturated fatty acids were convented from linolenic acid 18:3 n-3.

인삼, 들깨 기름, 칼슘 및 마그네슘이 함유되어 있는 식이로 사육한 토끼의 혈장 콜레스테롤과 HMG-CoA reductase의 활성을 조사하였다. 간에 포함되어 있는 HMG-CoA reductase의 활성은 인삼과 들깨 기름 및 마그네슘 첨가 식이군에 있어서 증가되었으며 혈장 콜레스테롤의 농도는 감소되었다. 혈장 LDL-콜레스테롤은 대조군보다 감소하였으나 HDL-콜레스테롤은 증가되었다. 또한 베타 글로블린의 분포도 인삼과 마그네슘 및 들깨 기름을 첨가 급여한 실험군에 있어서 가장 낮았으며, α-2-글로블린은 대조군에 비하여 가장 높은 값을 보였다. 전해질은 K+은 인삼 첨가로 감소 되었으나 Na+은 증가되었다. 혈장 마그네슘의 농도는 인삼의 첨가로 변화가 없었지만 칼슘과 들깨 기름 및 인삼 첨가식이로 마그네슘의 농도가 더 증가되었다. 혈장 칼슘의 농도는 마그네슘과 인삼을 첨가한 식이군에 있어서 감소하였다.

Because of its more complete hydrolysis and rapid absorption, MCT is expected its usefulness in the diets of patients with malabsorption syndrom. Also, several authors reported that serum cholesterol level was lower after MCT feedings. In this study, rate of each experimental group were fed for 4weeks with 20％ MCT, 20％ corn oil, mixed diet of 10％ MCT and 10％ corn oil, mixed diet of 17％ corn oil and 3％ shortening. After experimental diet, it was measured to growth rate and serum cholesterol, triglyceride and phospholipid level. The results were as follows; 1. The body weight gain was the lowest in MCT group and others were lower than control group. 2. Serum total cholesterol level was the lowest in corn oil group and others were significantly lower than control group. Free cholesterol level was lower in all experimental groups than control group too. 3. Serum HDL-cholesterol level was significantly higher in all experimental groups than control group. 4. The ratio of VLDL, LDL-Cholesterol to HDL-Cholesterol was significantly lower in all experimental groups than control group. 5. Serum triglyceride level was higher in all experimental groups than control group. Serum phospholipid level was significantly in only mixed diet group of corn oil and shortening than control group. 6. The ratio of total cholesterol to phospholipid was significantly lower in corn oil diet group than control group.

The purpose of this study was to find the effect of dietary polyunsaturated fat on of vitamin levels E, total cholesterol and HDL-cholesterol in serum of Spraque-Dawley male rats. Experimental rats were fed for 4 weeks with corn oil, perilla oil, lard at a level of 10% and 20% respectively. The results obtained are as follows; 1. Body weight gains were similar to all groups and food intake was significantly lower in all experimental diet groups than control diet group, especially 20% corn oil diet group was the lowest among the experimental diet groups. 2. Vitamin E levels in serum were significantly lower in 20% corn oil and 10%, 20% perilla oil diets groups than control diet group. 3. Serum total cholesterol levels were significantly higher in 20% perilla oil diet group which was significantly lower in vitamin E level than control diet group, and serum total cholesterol levels of 10%, 20% lard diets groups were significantly higher than control diet group. 4. Serum HDL-cholesterol levels were significantly lower in 20% perilla oil diet group which was significantly lower in vitamin E level than control diet group, and serum HDL-cholesterol levels of 10%, 20% lard diets groups also were significantly lower than control diet group.

The purpose of the study was to find an effect of Medium Chain Triglycerids (MCT) diet on cholesterol metabolism in rat. Sprague-Dawley rats were fed two different diets containing MCT(trioctanoate) and corn oil respectively. After feeding to each group for four weeks, the levels of serum and liver cholesterol, the excretion rates of fecal and biliary steroids, and also bile acid composition were investigated. The results obtained from the study are as follows : (1) The average body weight gain in MCT group was almost same as that in the corn oil group. (2) The concentration of serum cholesterol in MCT group was lower than that in the corn oil group. Therefore it is confirmed that the cholesterol lowing action of MCT diet was practically high. (3) The concentrations of liver cholesterol and Triglyceride in MCT group were almost same as that in the corn oil group. Therefore it is thought that the level of liver lipids was not influenced by the difference of diet in this study. (4) The excretion rate of fecal neutral steroid in MCT group was significantly lower than that in the corn oil group, while the rate of fecal bile acid excretion was about same in both MCT and corn oil group. (5) The composition rates of fecal bile acid such as cholic acid, chenodeoxycholic acid and deoxycholic acid, a secondary acid of cholic acid, in MCT group were significantly lower than that in the corn oil group. (6) The excretion rates of biliary cholesterol and bile acid in MCT group were significantly higher than that in the corn oil group, while the composition rates of biliary bile acid such as chenoddeoxycholic acid and deoxycholic acid in MCT group were significantly higher than that in the corn oil group.

Several authors reported that vegetable proteins cause to decrease serum cholesterol and reported the effect of limiting amino acid balance in dietary protein on the blood component. This study was designed to observe growth rate and blood component in 1% cholesterol-fed male rats intake pure isolated soy proteins and methionine for 4 weeks. The diets were supplemented with either 20% casein or isolated soy protein as dietary protein sources and methionine as limiting amino acid of isolated soy protein and casein protein. The results were as follows; 1) The body weight gain was the greatest in casein groups. 2) Serum total cholesterol concentration was significantly increased by casein groups and slightly decreased by isolated soy protein groups. 3) Serum HDL-cholesterol concentration was significantly increased by isolated soy protein groups than control group. 4) Serum total lipid and triglyceride concentrations were significantly decreased by isolated soy protein groups than control group. 5) Serum glucose concentration was significantly increased by isolated soy protein plus 0.8% methionine than control group. 6) Serum total protein and albumin concentrations were significantly increased by all experimental diets groups than control group. Especially, casein groups were the most increased in all experimental diets groups.

The purpose of this study was to investigate the effects of garlic diet on blood coagulation in cholesterol-fed rats. 36 male rats were divided into six groups and fed experimental diet for three weeks. Two-levels (2% and 4%) of fresh and boiled garlic were supplemented to the stock diet with 2% cholesterol and 0.25% bile salt for the experimental diet. After the rats fed with experimental diets during 3 weeks, the whole blood coagulation time, the content of plasma fibrinogen, the fibrinolytic activity and the prothrombin time were measured. The results are summarized as follows; 1. Garlic diet have no influence on body weight and food consumption in rats. 2. In the case of cholesterol-fed group, the whole blood coagulation time was decreased 24.3% compared with the normal group(A). But garlic diet group(C) was similar to the normal group(A) and groupD-F was increased 4.9-11.5%. 3. The content of plasma fibrinogen of cholesterol-fed group was higher 114.5% than that of normal gropu(A). In the garlic diets(C-F) the contents of plasma fibrinogen were increased 33.1-63.8%. 4. The fibrinolytic activity of cholesterol diet was decreased 16.2% compared with the normal group(A) but was increased 10.9-44.5% compared to the garlic diets(C-F). 5. The prothrombin time of cholesterol-fed group was decreased 8.9% compared with normal group(A) but was increased 8.0-46.3% in garlic diets(C-F). As mentioned above, the garlic diets increased whole blood coagulation time, prothrombin time and fibrinolytic activity and decreased the content of fibrinogen. For that reason it is thought that the garlic diets have a anticoagulation effect regulating the fibrinogen synthesis in liver.

이 실험은 yoghurt와 koumiss를 급여한 rat의 혈액중(血液中) -globulin과 cholesterol의 변화를 조사하기 위하여 실시하였다. Yoghurt와 Koumiss는 Lactobacillus bulgaricus와 Streptococcus thermophilus와 Saccharomyccs fragilis strain을 이용하여 환원유(還元乳)로서 제조(製造)하였다. 20마리의 rat을 완전 임의배치법에 의하여 4개의 군으로 나눈 후 각각 5마리씩 배치하였으며 실험구(實險區)는 대조구(對照區)와 milk, yoghurt 그리고 koumiss를 급여한 구(區)로 구분하였다. 실험결과(實驗結果)는 다음과 같다. Lactobacillus bulgaricus, Streptococcus thermophilus 그리고 mixed strain은 에서 8시간 배양(培養)하였으며 배양물(培養物)의 pH는 3.7, 4.6, 3.5로 각각 나타났다. Koumiss의 평균(平均) alcohol생산(生産)은 1.2%로 나타났다. Yoghurt와 Koumiss의 평균점도(平均粘度)는 총고형분함량(總固形分含量) 11%의 milk에서 각각 1500cp와 390cp를 나타내었다. Yoghurt와 Koumiss를 급여한 rat의 혈액중(血液中) -globulin 함량(含量)은 대조구(對照區)와 milk를 급여한 구(區)보다 높았다. Yoghurt와 Koumiss를 급여한 rat의 혈액내(血液內)에서의 cholesterol함량(含量)은 대조구(對照區)와 milk 구(區)보다 낮은 수준을 보였다.

Our country has been produced much amounts of panax ginseng roots which has a stimulating effects on the metabolism of protein, lipid and nucleic acids in the body. And the leaf trunk of panax ginseng were also produced a considerable amounts as the by - products. Therefore, this study was devised to observe the nutritional effect to rats feeding of rice diet supplemented with by - products of panax ginseng, male Albino rats of pure strain weighing 73.8 ± 0.7 g were used as experimental animal to investigate the changes of cholesterol in heart and testis. The animals were divided into sixteen diet group, they were the protein contents of 9%, 12%, 15% and 18% supplemented with 2% panax ginseng roots and its by - products respectively. The group without the supplements were used as the control. The diet group were again divided into 2 groups according to the feeding terms, 4 weeks and 8 weeks. It is concluded that the free from cholesterol and total cholesterol contents in the heart and testis with the supplements of panax ginseng roots and its by - products showed significant difference compared to the control group.

The effects of dietary magnesium, calcium on the serum cholesterol concentration in rabbit was studied for a period of 21 days using isocalorids and isonitrogenous basal diet. It is investigated that the serum cholesterol level lowering by feeding with calcium, magnesium, sesame oil and perilla oil, did not appeared but perilla oil and magnesium feeding group appeared a little bit lowering. There was no effect for the total protein, but there was some sort of effect for albumin and globulin. Particulary, alpa-globulin was increased by calcium, magnesium, sesame oil and perilla oil feeding groups. The esterified cholesterol was increased at the magnesium and perilla oil diet group. It is also, investigate that there is almost no effect for the electrolytes concentration and transport phenomena in the cell through magnesium, calcium, sesame oil and perilla oil diet groups.

The present study was designed to compare the effect of soybean oils and corn oils on serum levels of total cholesterol, HDL-cholesterol and free cholesterol in cholesterol-fed rats. The results are as fellows: 1. The average body weight gain in soybean oil diet group was lower than that of the control group. 2. The food efficiency ratio in soybean oil diet group was lower than corn oil diet group. 3. The serum levels of total cholesterol in soybean oil diet group than that in corn oil diet group. 4. The serum levels of free cholesterol in soybean oil diet group was lower than that in corn oil diet group. 5. The serum levels of HDL-cholesterol in soybean oil diet group than that in corn oil diet group. 6. The serum levels of VLDL-, LFL-cholesterol in soybean oil diet group was lower than that in corn oil diet group.

백쥐의 사료(飼料)에 석이버섯의 수용성(水溶性) 및 EtOH 추출물(抽出物)을 지(知)하여 지질대사(脂質代謝) 및 cholesterol대사(代謝)에 미치는 영향을 검토(檢討)한 결과(缺課), 다음과 같은 결론(結論)을 얻었다. (1) 간희(肝曦) 및 혈(血) cholesterol 저하작용(低下作用)에 관해서는 수용성(水溶性) 추출물(抽出物)이나 EtOH 추출물(抽出物)이 동시(同時)에 유핵(有劾)하다는 결과(結果)를 얻었다. 그리고 EtOH 추출물(抽出物)이 수용성(水溶性) 추출물(抽出物)보다 약간 높은 경향을 보였다. (2) 이 cholesterol 저하작용(低下作用)에 관여하는 유핵성출(有劾性物)은 물이나 EtOH의 추출(抽出)에 따라서 어느 쪽의 추출액으로도 이행(移行)되는 성질(性質)을 가지고 있다고 추정(推定)된다.