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        검색결과 227

        121.
        1997.05 KCI 등재 구독 인증기관 무료, 개인회원 유료
        A variation of free sugar and free amino acid contents of apples during the ripening period was determined by HPLC and amino acid analyzer. The research results are as followes: Fructose, glucose and sucrose were present in the apples. The contents of fructose was the highest, followed by the order of sucrose and glucose. The contents of total sugar in Red fuji was relatively higher than that of Fuji. During the ripening period, the contents of fructose increased conspicuously for the harvesting season and then it decreased gradually. The contents of glucose increased gradually for the harvesting season and then it somewhat decreased. The contents of sucrose increased continually, while, the contents of total free sugar reached the maximum for the harvesting season and then it decreased gradually. A variation in the contents of free amino acid and composition during the ripening period didn't make a significant difference. The major free amino acid of the apples was in the order of aspartic acid, serine, threonine, glutamic acid, histidine, arginine, and alanine. During the ripening period, aspartic acid, threonin, serine, alanine, histidine, and arginine increased gradually. Especially, alanine, histidine, and arginine increased remarkably after the the harvesting season. Glutamic acid, valine, isoleucine, and leucine didn't show much change. The contents of total amino acid increased little by little as the apples ripened, dranstically increased during the harvesting season and slowly increased or slightly decreased after the harvesting seasom.
        4,000원
        122.
        1997.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        This study was carried out in order to analyzed the changes in nitrogen containing taste compounds of three different types of traditional Korean soy sauce with varing ripening period, and to investigates correlation between sensory characteristics and taste compounds contents via contents assay and sensory evaluation of soy sauce samples. Total nitrogen, ammonia type nitrogen and amino type nitrogen contents showed the highest value in Kyupjang. 17 kinds of free amino acid was detected in Chungjangs and 16 kinds of free amino acid was detected in Kyupjang. Nucleotides and their related compounds detected were hypoxanthin, xanthin, IMP, AMP, Inosine, ADP. Free amino acid and nucleotides and their related compounds contents were highest in Kyupjang. Nitrogen related compounds content of high concentration soy sauce ripened over 150 days increased similarly with Kyupjang. In the sensory evaluation of soy sauce taste, Chungjang samples acquired the highest score in the offensive taste test while Kyupjang marked highest score for sweet taste, nutty taste, taste preference. High concentration soy sauce ripend over 150 days yielded the result similar to that of Kyupjang. Sweet taste showed positive correlation with nitrogen compounds. The materials that showed positive correlation with nutty and traditional soy sauce taste and taste preference were nitrogen compound, IMP, AMP. Except for tryptophan, all free amino acid showed positive correlation with nutty and traditional soy sauce taste. Particulary, taste preference correlated to lysine, aspartic acid and glutamic acid.
        4,000원
        125.
        1995.10 KCI 등재 구독 인증기관 무료, 개인회원 유료
        Essential and non-essential amino acids supplemented to culture medium stimulate mammalian embryo development in vitro. Amino acids such as glycine, taurine and alanine are concentrated in the lumen of oviduct and uterus and it can he thought that these amino acids may have physiological role on fertilization and embryo development. Our aim of this experiment was to investigate the effects of essential and non-essential amino acids, taurine or glycine supplemented to fertilization medium on the cleavage and subsequent in vitro development of bovine oocytes matured and fertilized in vitro. Immature oocytes were obtained from slaughtered Holstein cows and heifers and matured in TCM199 containing 10% fetal calf serum, 2.5 g /mL of FSH and LH and 1 g / mL of estradiol with granulosa cells in vitro. After maturation, oocytes were coincubated with sperm in fertilization medium supplemented with Minimum Essential Medium (MEM) essential and non-essential amino acids, taurine (3.75 mM) or glycine (10 mM) for 30 hours in vitro. Inseminated oocytes were cultured in synthetic oviduct fluid medium (SOEM) containing MEM essential, non-essential amino acids and 1 mM glutarnine up to 8 days after fertilization.Supplementation of fertilization medium with MEM essential and non-essential amino acids lowered significantly (p<0.05 and p<0.001) the cleavage rate after 30 hours of IVF (53.3%) and at Day 3 (62.7%: Day 0: the day of I VF) compared to control (64.3% and 77.3%, respectively). Subsequent developmental rates to morulae (Mo) and expanding blastocysts (ExBL) also significantly decreased (p<0.001 and p<0.05 for Mo and ExBL) when oocytes were coincubated with sperm in the medium containing MEM amino acids. Taurine added to fertilization medium have not increased the cleavage rate over the control, whereas glycine showed significantly lower (p<0.01) cleavage rate at Day 3 than that of taurine, but there was no significant difference in the developmental rates to Mo and ExBL of bovine embryos irrespective of the supplementation of taurine or glycine to fertilization medium. In conclusion, supplementation of fertilization medium with essential and non-essential amino acids, taurine or glycine has no beneficial effect on in vitro cleavage and development of bovine oocytes matured and fertilization in vitro.
        4,000원
        126.
        1995.09 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The study was carried out to compare the factors of overall preference in the sensory test to the analyzation of some compositions in the 7 kinds of wild grasses : An Evening Primerose, a Spiderworts, the flower of a Convolvulus, So Ru Jang Yi, Shoe Bi Rum. O Yi Pul, Jip Sean Na Mul. Results were summaried as follows: 1. The Tannin contents of fresh sample and cooked samples were determined as 0.27∼2.4g%, 0.25∼1.43g% respectively. The largest amount of fresh samples was contained in a Shoe Bi Rum. The smallest amount of them was in an Evening Primerose. The highest level of cooked samples was found in a Shoe Bi Rum, and the lowest was in the O Yi Pul. These results were similar to sensory test. 2. The free amino acid contents of 2 kinds of samples were determined as 25.15∼179.5㎎%, 1.86∼13.6㎎% respectively. The largest amount of sweet taste of them was O Yi Pul and So Ru Jaeng Yi respectively. But So Ru Jaeng Yi is not appeared sweety becase this have much tannin. The smallest amount of sweet taste was a Spiderwort. The highest level of bitter taste was So Ru Jaeng Yi the and lowest was Jip Sean Na Mul. Among of them Jip Sean Na Mul is similar to organoleptic test but So Ru Jaeng Yi is not strong bitter taste in sensory evaluation. The highest level of sour taste of cooked samples was So Ru Jaeng Yi and the lowest of them was Shoe Bi Rum. 3. The reducing sugar contents of fresh sample and cooked samples were determined as 1.80∼4.9g%, 1.84∼3.57g% respectivley. The largest of fresh samples were So Ru Jaeng Yi and the lowest was Shoe Bi Rum. The highest of cooked samples were an Evening Primerose and the lowest was a Convolvulus. Among of these results an Evening Primerose was not similar to sensory test because it has much other components. 4. The level of chlorophyll of fresh samples and cooked smaples were determined as 11.7∼39㎎%, 11.3∼40.3㎎% respectively. The highest of fresh samples was Shoe Bi Rum and the lowest was Jip Sean Na Mul. The largest of cooked samples was So Ru Jaeng Yi and the lowest was a Jip Sean Na Mul. Amoung of these results the Jip Sean Na Mul was not similar to the sensory test and the other samples
        4,000원
        127.
        1995.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The amino acid sequence of basic isozyme E5 of Horseradish Peroxidase(HRP E5) was determined by protein sequencing. HRP E5 consisted about 300 residues, and has a molecular weight of approximately 36, 000±500 dalton. The protein was rich in aspartic acid (14%), arginine (13%), and leucine(ll%). The primary structure of HRP E5 was established by sequencing its tryptic(T_1-T_19) and lysylendopeptic(A_1-A_3) peptides. The sequence homology between HRP E5 and HRP C(neutral isozyme of horseradish peroxidase) is found to be more than 66%. The highest concentration of identical residues are found on residues 29∼56, 90∼123, and 155∼173, but relatively low on 174∼271.
        4,000원
        128.
        1994.11 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The metal complexes containing amino acidic ligands were prepared by using 11 kinds of amino acids as ligands and Ni, Cu, Co, Zn, Fe as a central metal. The starting was continued for 4hrs at room temperature. But Bis(D,L-Serine)Ni (II), and (D,L-Serine)Co (II) were prepared by heating method(80℃). In order to investigated reaction activity of Bis(D,L-Aspartato) Metal(II), stirring time was varied and Bis(D,L-Tyrosine ) Metal(II) used different divalent metal salts. We anticipate getting a great value from these prepared complexes as a monomer and a catalyst of polymerization which has peculier characteristics.
        4,500원
        129.
        1994.09 KCI 등재 구독 인증기관 무료, 개인회원 유료
        본 연구에서는 전기투석법을 이용하여 아미노산 수용액중에 존재하는 전해질인 무기염의 분리 정제에 관하여 고찰하였다. 생물 분리기술로서 아미노산의 등전위점을 이용한 전기투석법의 적합성을 검토하기 위하여 음이온교환막, NEOSEPTA AM1(Tokuyama Soda Co. Ltd., Japan)과 양이온교환막 CM1 (Tokuyama Soda Co. Ltd., Japan)막을 사용한 전기투석조를 설계하였고 아미노산 발효공정과 유사한 계를 만들어서 무기염인 NaCl의 분리실험을 하였다. 즉, 운전조건을 설정하기 위해 pH에 따른 아미노산의 누설량, 한계전류 밀도를 측정한 후, 이를 바탕으로 회분식과 연속식 실험으로 염의 제거량과 아미노산의 누설량을 정량적으로 고찰하고 전류효율을 구하였다. 회분식 장치에서 아미노산 수용액별로 11시간동안 운전한 결과 NaCl의 제거효율은 96.1~96.2%이었고, 아미노산의 누설율은 glycine의 경우 2.5%, methionine의 경우 1.7%, alanine의 겨우 2.0%이었으며, 전류효율은 44.5~44.6% 범위이었다. 연속식 장치에서는 아미노산 수용액별로 dilution rate을 1.0~3.9h-1로 변화시켜 실험한 결과, 120~150분 사이에 정상상태에 도달하였고, 유속이 작을수록 염의 제거 효율은 증가하였으나 전류효율은 감소하였다. 이때 아미노산의 누설은 거의 없었다.
        4,000원
        130.
        1994.06 KCI 등재 구독 인증기관 무료, 개인회원 유료
        쑥은 옛부터 민간요법 및 한약제의 원료와 구황식품으로 사용해 온 야생식물이다. 본 연구에서는 쑥의 유용식물 자원으로서의 중요성을 알아보기 위하여 쑥첨가량을 0, 10, 20, 30, 40%로 하여 쑥설기와 쑥절편을 제조하여 아밀로스 함량을 정량하였고 가장 선호도가 높은 30% 쑥설기와 쑥절편의 아미노산 함량을 분석하였다. 30% 쑥설기의 총 유리아미노산 함량은 25.90㎎%로 백설기보다 약 4배 이상 많은 함량이었고, 30%쑥절편의 총 유리아미노산의 함량은 39.58㎎%로 백절편보다 약 11배 이상 많은 함량으로 아미노산 보강효과가 크다고 사료된다. 쑥첨가량에 따라 쑥설기와 쑥절편의 노화를 일으키는 아밀로스가 대조구에 비해 쑥설기는 7.58∼13.35%, 쑥절편은 0.82∼8.34%정도로 감소하였다.
        4,000원
        133.
        1992.12 KCI 등재 구독 인증기관 무료, 개인회원 유료
        여름철에 제조한 재래식의 벽돌형 메주(A)구, 국균접종의 벽돌형 메주(B구)와 콩알형 메주(C구)로 하절기에 간장을 담금하고 숙성과정중의 유리아미노산을 분석한 결과는 다음과 같다. 숙성기간과 시험구에 따라 다소 차이는 있으나 담금직후에 lysine, histidine, serine, glycine, valine, methionine, phenylalanine이, 90일에는 aspartic acid, glutamic acid, alanine, threonine, arginine, leucine 등이, 180일에는 isoleucine, tyrosine이 각각 검출되었다. Lysine과 histidine은 숙성과정 중 또는 시험구에서 증가 현상이 컸고, 검출된 유리아미노산 중 가장 함량이 높았다. Glutamic acid, aspartic acid, methionine 등은 숙성기간 중 감소하였다. 유리아미노산 총량은 숙성기간의 경과에 따라 증가되어 180일에 3,559-4,119mg/100ml으로 가장 높은 함량을 나타내었다. 국균접종의 콩알형 메주로 담금한 C구가 유리아미노산 총량이 가장 높았고 벽돌형 메주의 A구와 B구는 비슷하였다. 총질소나 아미노태질소는 벽돌형 메주로 담금한 간장이 높았다.
        4,000원
        135.
        1991.12 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The blocked N-terminus and N-terminal sequence ol soybean β-amylase were determined by analyzing the acidic peptides derived on peptic digestion of the enzyme. The acidic peptides were separated from the digest on a Dowex 50×2 column(1×5㎝) and purified by reversed phase-high performance liquid chromatography(RP-HPLC). The major acidic peptide, PEP-1, was a heptapeptide. The N-terminal 7 amino acid sequence of soybean β-amylase was deduced to be acetyl-Ala-Thr-Ser-Asp-Ser-Asn-Met- from the results of sequence analysis of PEP-1 and amino acid analysis of other acidic peptides.
        4,000원
        136.
        1990.12 KCI 등재 구독 인증기관 무료, 개인회원 유료
        General components, amino acids and minerals contained in 7 kind of edible wild grasses were estimated. Samples were collected from the field of Kapyong area, Kyung-ki, Korea. Results of the estimation were summarized as follows ; 1) A relatively large amounts of protein and fat were contained in the edible parts of each sample. 2) The most abundant amino acids in the edible parts of each sample were glutamic acid (26.6%), serine (18.7%), valine (22.4%), respectively. 3) A high level of calcium and iron was found in the edible parts of each samples. Especially calcium was abundant in the leaves of Rumex coreanus Nakai and iron was abundant in the Commelina communis L. Both copper and zinc were abundant in the Sanguisorba officinalis L.
        4,000원
        138.
        1989.06 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The compositions of total and free amino acids in parts of Omija were investigated. The most abundant amino acids in fruits, endocarps, and seeds were arginine (50.80%), lysine (14.37%), glutamic acid (14.22%), respectively. Since the amino acid scores of fruits, endocarps, and seeds were 9.4, 11.9, and 16.7, respectively, the limiting amino acid of each part were S-compound amino acids. In the composition of free amino acids, contents of lysine were highest one such as 51.78, 57.00 and 32.88% in fruits, endocarps and seeds, respectively. The contents of histidine from free amino acids were 23.62% in fruits, 22.37% in endocarps, and 26.41% in seeds.
        3,000원
        139.
        1988.11 KCI 등재 구독 인증기관 무료, 개인회원 유료
        In order to investigate the amino acid and fatty acid content in Thuja biotae water extract treated with alkaline, it was performed. There are 16 kinds of different amino acid and 20 kinds of different fatty acid in Thuja biotae water extract. An aspartic acid was contained 52% and proline was contained 10%, particulary, r-aminobutyric acid was analysed. Essential fatty acids; linoleic acid, linolenic acid and arachidonic acid were cotained a lot amount. There are 11 different unknown materials which were identified by GC-MS spectrum, such as N-[(4α,5α)-cholestan-4-yl]-acetamide; 22,26-Epithio-furost-5-en-3-ol; 2-Methyl-6-(4-methyl-3-cyclohexen-1-yl)-4-heptanone; 3,12,14-Tris(acetyloxy)-pregnane-15,20-dione;22-Methyl-26-thio-furost-5-en-3-ol; 7-Ethenyl-1,2,3,4,4a,4b,5,6,7,9,10,10a-dodecahydro-1,4a,7-trimethyl-1-phenanthrene carboxyaldehyde; Methoxyiminopro-panedioic acid; 13-Methyl-13-β-Methyl-13-vinyl-dodecaarp-7-en-3-3-ol; 22-Methyl-26-thio-furost-6-methyl-3-ol; 5α-Androstane-2,11-dione; 9-Methyl-heptadecanoic acid.
        4,000원
        140.
        1986.05 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The composition of fatty acid and amino acid for Seong-ge-al(sea urchin) were analysed by the automatic analyser. There are two kind of samples such as raw and saited, fermented one. The results obtained are as follows. 1. In the general composition, crude protein, crude lipid and ash as 15.8%, 3.5% and 2.2%, respectively. The raw sample showed pH 5.6 value. 2. In case of amino acid, it contained 17 different kind of amino acids. Among them, a lot of glutamic acid, aspartic acid, arginine, glycine and valine were contained. 3. In case of fermentation period, threonine, histidine, lysine and arginine were increased a little bit. 4. In case of fatty acid, it contained 13 different kind of fatty acids. Among them, a lot of palmitic acid, behnic acid, linoleic acid and arachidonic acid were contained.
        4,000원