The status of probiotics is increasing worldwide and the range of application of probiotics is also being extended to the livestock industry. The use of probiotics in the swine industry aims to improve animal health and productivity by forming a healthy gut microbiota. Intensive pig breeding, a common breeding method in modern society, causes physiological stress in pigs, resulting in imbalance of the intestinal microbiota and dysfunction of the intestinal barrier. We conducted a scientific research on the properties of probiotics for multi-strain probiotics (MSP) and observed the economic benefits and efficiency of culturing MSP through a self-cultivation system in livestock. Initially, we observed alteration occurred by MSP application in the gut microbiota of pig. MSP were characterized to have resistance to digestive juices such as gastric acid and bile, followed by colonization in the target organ, the gastro-intestinal tract (GIT). In addition, MSP was also confirmed to have antibacterial ability against pathogenicity that most frequently infected in the swine industry and showed low resistance to antibiotics which means guaranteed stability when added to feed. The growth rate of probiotics in the optimized medium used in the self-cultivation system was suitable for efficiently and economically culturing and feeding pigs with high concentrations of probiotics, considering the ingredient price and the growth efficiency. We observed the significant alteration of gut microbiota of pig by application of MSP. Importantly, the MSP supplementation significantly increased the beneficial bacteria genus of Bifidobacterium that confer a health benefit to the host in pig GIT, whereas decreased the number of harmful bacteria including coliforms. In addition, MSP influenced on the uniformity of gut microbiota at both of sow and weaning pigs. Taken together, the application of MSP with high concentration of probiotics using self-cultivation system may critically improve the pig health by regulation intestinal microbiota.

브로일러의 생산성과 닭고기의 품질에 미치는 Bacillus. subtillus, Streptomyces galilaeus 및 Sphingobacteriaceae로 구성된 사료용 복합생균제(PFM, probiotic feed mixture)의 효과를 조사하였다. 병아리 (Ross 308) 240마리를 4개의 처리구로 나누어 35일간 사육하였다. 처리구는 사료용 복합 생균제를 함유하지 않은 대조구(CON, control), 일반생균제(CP3, commercial probiotics 0.3%), 사료용 복합 생균제 3(PFM3, 사료용 복합 생균제 0.3%), 사료용 복합 생균제 5(PFM5, 사료용 복합 생균제 0.5%)로 구분하였다. 체중은 CON과 비교할 때 CP3, PFM3, PFM5가 높았고 PFM3, PFM5는 CP3에 비해 우수하였다(p<0.05). F낭, 흉선, 비장의 무게 및 IgG 수준은 CON과 비교할 때 PFM3, PFM5 및 CP3에서 증가하였으며 PFM3, PFM5는 CP3에 비해 높았다(p<0.05). 맹장의 Lactobacillus 균수는 PFM3, PFM5 및 CP3를 섭취한 브로일러가 CON에 비해서 높았으나 E.coli, Salmonella, coliforms, 총 호기성 균수는 감소하였다(p<0.05). PFM3 및 PFM5를 섭취한 닭가슴살의 보수력은 CON 및 CP3와 비교할 때 증가하였다(p<0.05). 결론적으로 Bacillus subtillus, Streptomyces galilaeus 및 Sphingobacteriaceae를 포함하는 사료용 복합생균제 0.3%가 브로일러의 생산성과 닭고기 품질향상에 기여할 수 있음을 나타낸다.

본 연구는 계란 생산성, 계란품질 및 악취저감에 관한 Bacillus subtillus, Streptomyces galilaeus 및 Sphingobacteriaceae로 구성된 복합생균제(PM, probiotic mixture)의 효과를 조사하였다. 산란계 Hy-Line Brown 240수를 4처리구로 구분하여 6주 동안 사육하였다. 처리구는 복합생균제를 함유하지 않은 대조구(CON, control), 일반생균제(CP3, commercial probiotics 0.3%), 복합생균제 3(PM3, 복합생균제 0.3%), 복합생균제 5(PM5, 복합생균제 0.5%)로 구분하였다. 산란율, 계란 호우유니트, 난각두께, 난각강도, 난황색 및 농후난백 높이는 PM3, PM5가 CON 및 CP3에 비해서 높았다. 계분으로부터 암모니아, E. coli, 총호기성균, Coliform, Salmonella 균수는 CON>CP3>PM3>PM5 순서로 증가하였다. 본 결과는 B. subtillus, S. galilaeus 및 Sphingobacteriaceae로 구성된 복합생균제 0.3%가 산란율, 계란품질 개선 및 분에서 암모니아 발생을 낮추어 양계장 악취저감 효과를 갖는다는 점을 보여 준다.

This study was conducted to determine the effects of dietary probiotics BiopowderR (BP) on pig performance, carcass characteristics and ammonia concentration in feces. A feeding trial was conducted with a total of 36 growing pigs of an average 29kg body weight. Each treatment had 4 pigs with 3 replications. Pig during grower (26days) and finisher periods (62days) were fed 0.15 and 0.30% concentrations of BP respectively. Body weight gain slightly increased for pigs fed BP during overall periods compared with control, not significantly different. Especially, in growing periods, the body weight gain of BP treatment increased by 10% compared with control (700g vs 775g), in finishing periods, that of BP 0.3% treatment increased by 10% compared with control (890g vs 980g), not significantly different among treatments. Feed intake slightly increased for pigs fed BP in overall periods. During grower periods, the feed intake of BP 0.3% treatment increased by 11.0% more than control, but also not significantly different among treatments. Feed efficiency was slightly improved for pigs fed BP overall periods when compare to the control, but not significantly different among treatments. In feces, average ammonia concentration (ppm) and bacterial count (log cfu/g) were not affected by BP addition. There was no significantly different in carcass weight, fat thickness and carcass grade. In conclusion, supplemental BP tended to slightly improve the performance of pig and did not influenced the concentration of ammonia in feces and carcass characteristics.

This study was conducted to investigate the efficacy of mixed probiotic on the immunity, productivity index and mortality rate in the broiler. Total of 120 one-day-old Ross broilers chicks were randomly assigned into two treatments (control dietary group and probiotic-treated group) with three replications of each treatment. The probiotic group broiler had a lower mortality rate than control during the experimental period. The productivity index in the probiotic group increased significantly than the control group. The weight of the bursa of fabricius was high in the probiotic-treated group than the control group. Activated the immunity level after fed the probiotic mixed diet compared to the control group. Furthermore, the probiotic diet significantly decreased the saturated fatty the control group. Whereas the probiotic mixed diet increased the unsaturated fatty acid than the control group. Afterward, the diet including probiotic induced positive impact on broilers immunity level. This indicates that a probiotic mixed diet could be protecting the intestine from the invasion of a pathogenic organism. It would be beneficial to the poultry industries by decrease the broiler mortality rate with elevated the immunity.