산양삼은 산림청 특별관리 임산물로 지정되어 관리되고 있지만 체계적인 연구 나 표준 재배지침이 현장의 재배 현실과는 상이한 부분이 많은 실정이다. 이에 본 연구에서는 평창지역의 산양삼 재배지 환경조건에 따른 진세노사이드 함량이 어 떠한가를 알아보기 위하여 산양삼 시료를 각각 10본씩 채취하여 실험을 통해 확 인하였다. 산양삼 입지환경은 경사, 사면 방향, 해발고, 나무 종류, 흉고 직경과 수 고 등 모두 6가지를 조사하여 국립산림과학원의 분류기준을 참고하여 침엽과 활 엽으로 구분하였다. 산양삼 재배지의 토양산도를 포함한 9가지 토양이화학성 분석 에서는 전체적으로 침엽지역보다 활엽지역에서 높은 함량을 보였다. 통계분석 결 과에서는 A, B, C, 재배지에서 침엽과 활엽지역 간 통계적 유의성이 있는 것으로 나타났으며, D 재배지는 침엽과 활엽지역의 유의차가 거의 없는 것으로 나타났다. 진세노사이드 함유량 분석 결과에서는 Re, Rb1, Rg1의 순서로 높은 함량을 나타 내고 있어서 평창 산양삼의 주성분을 확인할 수 있었다. 연구 결과로 산양삼은 산 삼이 잘 생육할 수 있는 환경과 비슷한 곳을 재배지로 선정하여야 고품질의 제품 을 생산할 수 있다고 예상할 수 있다. 향후 본 연구 결과가 임업인 소득증대에 도 움을 줄 수 있는 자료로도 활용될 수 있기를 기대한다.

This study was conducted to determine the changes in ginsenosides content according to additional UV-A, and UV-B LED irradiation before harvesting the ginseng sprouts. One-year-old ginseng seedlings (n=100) were transplanted in a tray containing a ginseng medium. The ginseng sprouts were grown for 37 days at a temperature of 20°C (24h), a humidity of 70%, and an average light intensity of 80 μmol·m-2·s-1 (photoperiod; 24h) in a container-type plant factory. Ginseng sprouts were then transferred to a custom chamber equipped with UV-A (370 nm; 12.90 W·m-2) and UV-B (300 nm; 0.31 W·m-2) LEDs and treated for 3 days. Growth parameters and ginsenoside contents in shoot and root were conducted by harvesting on days 0 (control), 1, 2, and 3 of UV treatments, respectively. The growth parameters showed non-significant differences between the control and the UV treatments (wavelengths or the number of days). Ginsenoside contents of the shoot was highly improved by 186% in UV-A treatment compared to the control in 3 days of the treatment time. The ginsenoside contents of the roots was more improved in UV-A 1-day treatment and UV-B 3-day treatment, compared to the control by 171% and 160%, respectively. As a result of this experiment, it is thought that UV LED irradiation before harvesting can produce sprout ginseng with high ginsenoside contents in a plant factory.

Compound K (20-O-β-(D-glucopyranosyl)-20(S)-protopanaxadiol)는 진세노사이드의 활 성성분이다. Compound K는 경구 투여 후 Rb1, Rb2 및 Rc가 사람의 장내 미생물의 β-glucosidase에 의 해 생물전환 과정을 거쳐 생성된다고 알려져 있다. 본 연구는 생물전환된 인삼농축액에서 얻은 compound K를 이용해 항염증 및 독성을 조사하였다. 세포독성평가 결과, compound K는 0.001∼1 μg/mL의 농도 범위에서 유의적인 세포독성은 나타나지 않았으며, LPS로 염증이 유발된 RAW 264.7 세포에서 TNF-α, MCP-1, IL-6 및 NO의 생성을 억제하는 것으로 확인되었다. 동일 농도범위에서 TNF-α 및 IFN-γ로 염증이 유발된 HaCaT 세포는 compound K의 처리로 인해 IL-8의 생성을 감소시키는 것으로 나타났지만, IL-6의 경우 일부 농도에서 생성을 감소시켰으나, 통계적인 유의성은 나타나지 않았다. Brine shrimp를 이 용한 치사율 검정법에서 compound K의 LC50는 0.37mg/mL로 다소의 독성을 함유하고 있는 것으로 나타 났으나 compound K가 35% 고함유된 생물전환물은 LC50가 0.87mg/mL로 나타나 상대적으로 낮은 독성 을 보였다. 따라서 이 생성물은 향후 여드름 완화용 화장품 개발에 사용할 수 있는 매우 우수한 기능성 소 재가 될 수 있을 것으로 기대된다.

This study was carried out to extract ginsenosides in by-products from honeyed red ginseng. Response surface methodology (RSM) was used to optimize the extraction conditions. Based on D-optimal design, independent variables were ethanol (extraction solvent) concentration (30-90%, v/v), extraction temperature (25-70oC), and extraction time (5-11 h). Extraction yield (Y1) and total ginsenosides (Y2) in the extract were analyzed as dependent variables. Results found that extraction yield increased with increasing extraction temperature and time, whereas it was decreased with increasing ethanol concentration. Similar trends were found for the content of ginsenosides in the extracts, except for ethanol concentration, which was increased with increasing ethanol concentration. Regression equations derived from RSM were suggested to coincide well with the results from the experiments. The optimal extraction conditions for extraction yield and total ginsenosides were an extraction temperature of 56.94oC, ethanol concentration of 57.90%, and extraction time of 11 h. Under these conditions, extraction yield and total ginsenoside contents were predicted to be 84.52% and 9.54 mg/g, respectively.

저탄소 공정을 이용한 추출 기술인 초음파, 마이크로파 및 초고압 추출 공정기술의 이산화탄소 배출량(TCO2)과 얻어진 저분자 진세노사이드 총량의 상관관계를 비교하였다. 기존의 공정인 열수 추출 공정의 TCO2 배출량은 약 0.4 TCO2로 나타났다. 마이크로파 추출 공정의 경우 0.1437 Ton 당 CO2를 배출하는 것으로 확인 되었다. 또한, 초음파 추출 공정의 경우 0.0862 Ton 당 CO2를 배출하는 것을 확인 하였으며, 초고압 추출 공정의 경우 0.1014 Ton당 CO2를 배출하는 것을 확인 하였다. 저탄소 공정별 저분자 진세노사이드의 전환된 양을 측정한 결과 마이크로파 추출 공정의 경우 약 246.65% 정도 증진된 것을 확인 할 수 있었다. 또한, 초음파 공정의 약 275.71% 증진된 결과를 보였다. 초고압 추출 공정의 경우에는 약 295.21% 증진된 결과를 얻었다. 전체적으로 열수 추출 공정의 경우 얻어진 저분자 진세노사이드가 적은 반면 CO2 배출량이 매우 높은 것을 확인하였다. 반대로, 저탄소 추출 공정인 마이크로파, 초음파 및 초고압 공정의 경우 얻어진 저분자 진세노사이드의 양이 높으며, 방출되는 CO2의 양이 기존의 재래 방법보다 적은 것을 확인 하였다. 따라서, 저탄소 추출 공정인 마이크로파, 초음파, 초고압 추출 공정을 통해 인삼을 효과적으로 추출을 할 수 있으며, 친환경 저탄소 공법을 통해 CO2 발생량을 억제하여 경제적으로 천연물을 추출할 수 있을 것으로 사료된다.

To study on antioxidant effects in the liver of 40-week-old mouse, the sample were orally pretreated 5mg/kg/day for 5 days with red ginseng saponin components(total saponin, protopanaxadiol saponin, protopanaxatriol saponin, ginsenoside-Rd, ginsenoside-Re, compound-K) for 5 days. The ability of saponin to protect the mouse liver from oxidative damage was examined by determining the activity of superoxide dismutase(SOD), glutathione peroxidase(GPx) and the contents of glutathione, the level of malondialdehyde, The only protopanaxadiol among the ginseng saponin fractions was significantly increased the hepatic SOD activity(p<0.01). The red ginseng saponin induced a slight increase of GPx activity, especially ginsenoside Rd, compound K and protopanaxatriol treatments significantly increased its activity. The content of glutathione was significantly increased by total saponin, protopanaxadiol and ginsenoside Rd(p<0.01), but the oxidized glutathione level was lowered in all the red ginseng saponin. Finally, the level of malondialdehyde was significantly decreased by ginsenoside Rd and protopanaxadiol. In conclusion, protopanaxadiol and ginsenoside Rd among the saponin fraction were especially increased in the activity of hepatic antioxidative enzyme and decreased the lipid peroxidation that was expressed in term of MDA formation. This comprehensive antioxidant effects of red ginseng saponin seems to be by a certain action of saponin other than a direct antioxidant action.

본 연구는 7년, 13년근 산양삼의 생육특성과 진세노사이드 (G) 함량 간의 상관관계를 구명하기 위하여 수행되었다. 6개소의 산양삼의 생육특성을 조사한 결과, 뇌두길이, 뿌리길이, 생중량, 단면적, 표면적, 부피에 있어 13년근 산양삼이 7년근 산양삼에 비하여 유의적으로 높은 것을 확인하였다. 진세노사이드 11종에 대한 함량은 G-Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, Rg2 함량이 13년근 산양삼이 7년근 산양삼 보다 유의적으로 높은 수치를 확인하였다. 또한 산양삼과 인삼(재배삼) 진세노사이드 함량 을 비교한 결과, 13년 산양삼에서 G-Rb1, Rd, Re, Rf, Rg1이 4 년, 5년근 인삼(재배삼)에 비해 유의적으로 함량이 높은 것으로 확인되었다. 산양삼 연근별 생육특성과 진세노사이드 함량 간의 상관관계를 분석한 결과, G-Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, Rg2 함량은 뇌두길이, 생중량, 단면적, 표면적, 부피와 유의정인 정의 상관관계를 보였으며, G-Rb1, Re, Rf, Rg2는 줄기직경 과 부의 상관관계를 확인하였다. 본 연구는 산양삼의 7년근과 13년근을 대상으로 생육특성과 진세노사이드 함량 상관관계를 구명함으로써 연근에 따른 품질규격 정립에 유용한 정보를 제공 할 것으로 판단된다.

Background : Ginseng (Panax ginseng C. A. meyer) is a medicinal herb which has been used for prevention and treatment of diseases in Korea, China and Japan for a long time. Ginseng is well known to have effects such as immunity enhancement, anticancer, antioxidative effect and blood circulation improvement. These pharmacological effects of ginseng have been reported to be due to various ginsenoside components. Recently, many studies have been actively conducted to identify the major components and functionalities of ginseng berry. Ginsenoside, a representative active ingredient of ginseng, is distributed in various parts of ginseng. However, contents of certain ingredient such as ginsenoside Re which have antidiabetic effect is higher in ginseng berry. This study was conducted to compare ginsenoside content and UV blocking effect according to the color of ginseng berry. Methods and Results : The ginseng berry extracts for the component analysis and UV-blocking effect test were obtained by harvesting the ginseng berry by color in 4-years-old ginseng cultivated, and then removing the seeds and extracting only the flesh of the ginseng berry and the peel with methanol. Ginsenosides analysis was carried out by dissolving 100 ㎎ of the extract in 2 ㎖ of methanol, filtering it with a 0.50 ㎛ filter and using it as a sample for HPLC analysis. Analysis of ginsenoside content showed that the total ginsenoside and ginsenoside Rb2 content of yellow ginseng berry were 23.9 and 2.9 ㎎/g, while those of red berry was 19.6 and 2.3 ㎎/g, respectively. The UV blocking effect of yellow ginseng berry was 17.4%, which was higher than that of red berry. Conclusion : The ginsenoside content and UV blocking effect of yellow ginseng berry were superior to those of red berry. These results suggested that yellow berry is more useful as cosmetic material for UV protection.

Background : Panax ginseng the king of Oriental medicines, has enormous potential against many of the disease symptoms and ailments. Panax quinquefolius is an another important member of Panax family which has similar benefits and medicinal properties. Both the species contains ginsenosides a pharmacologically active component abundant in their roots. In an effort to establish the new ginseng cultivars with improved agronomical characters such as root shape, stress tolerant and higher ginsenoside contents, the reciprocal interspecific hybrids were generated Methods and Results : Four elite cultivars and two new lines of ginseng were chosen to produce interspecies reciprocal hybrids with P. quinquefolius, by pollen dusting on the emasculated flowers. Among the F1 populations, the reciprocal hybrids generated by using new line 0837 both as maternal and paternal parent showed better properties and hybrid vigor. They showed strong root phenotypes with many lateral and fine roots thus having 10% to 20% higher ginsenoside contents compared to the parental populations. Among the major ginsenosides, the pharmacologically active ginsenosides such as Re, Rb1, Rb2 and F1 were enriched and accounted for the 70% of the PPD ginsenosides in 0837/Pq and Pq/0837 crosses, displaying strong dominance. Conclusion : Thus, based on our result we could conclude that P. ginseng line 0837 is the superior variety compared to the already existing lines for performing the interspecific hybrids with promising outcome in their root quality and ginsenoside content.

새싹삼의 유효성분 및 진세노사이드를 함유한 발효주 제조를 위해 3단계로 나누어 각 2일씩 발효 증량하면서 발효주를 제조 한 후 전처리 방법을 달리한 새싹삼(CO, 무첨가 대조군; GP 새싹삼 분쇄물 첨가; WEP, 새싹삼 물추출물; EEP, 새싹삼 효소분해 추출물)을 발효주의 총 무게 대비 6%씩 함유하도록 첨가하여 6일간 숙성시키면서 이화학적 특성을 분석하였다. 3단계까지의 담금 과정에서 알코올 함량은 담금 단계가 이어질수록 높아졌으며, pH는 낮아지고, 산도는 증가하는 경향이었다. 3단계 담금 후 6일간의 숙성 동안 알코올 함량은 서서히 높아졌으며, pH는 서서히 낮아지고 산도가 증가하였다. 숙성 6일 동안 환원당 함량은 감소하는 경향이었는데, 특히 숙성 2일에 급격하게 함량이 감소하였다. 총페놀화합물의 함량은 숙성이 진행됨에 따라 점차 증가하는 경향이었다. 발효주의 숙성 중 진세노사이드는 Re가 가장 높은 함량이었으며 다음으로 Rg1과 Rh1의 순으로 함량이 높았고, 그 외의 진세노사이드는 일부 시료에서만 검출되었으며, 그 함량도 낮았다. 숙성 초기의 진세노사이드 함량은 GP와 EEP가 WEP에 비해 더 높았으나 숙성기간의 경과와 더불어 점차 감소하여 숙성 6일후에는 WEP에서 총진세노사이드의 함량이 가장 높았다. 숙성기간 중 ABTS 라디칼 소거활성은 숙성 기간의 경과와 더불어 증가하는 경향이었으며, 숙성 6일에는 62.64-64.71%로 서로 간에 유의적인 차이가 없었다. 이상의 결과로부터 볼 때 담금이 진행 된 후 첨가된 새싹삼은 전처리 방법에 관계없이 발효주의 기초 품질에는 영향을 미치지 않았으나 진세노사이드 함량을 기준으로 볼 때 50℃ 정도의 저온에서 추출한 물추출물을 첨가하는 것이 가장 적절할 것으로 판단된다.

Background: This study was carried out to determine the best time for collecting ginseng berries without reducing the ginsenoside- Re content of ginseng roots, which are used as food, medicine, or cosmetic materials. Methods and Results: The test variety of ginseng used in this study was is Chunpung, which was collected from a 4-year-old ginseng field. Ginseng berries were collected at 7, 14, 21, 28, 35, 42, 49, and 56 days after flowering. The number of berry bunches per 1.62㎡ ranged from 43.4 to 61.4, while the weight of berries per 1.62㎡ was the greatest when they were collected 49 days after flowering. The root fresh weight per 1.62㎡ was increased by 0.21 - 1.00 ㎏ compared with that before the test, but root weight gain was decreased as the berry collection time was delayed. Total ginsenoside content of 4-year-old ginseng was the highest when berries were collected 7 days after flowering, while the ginsenoside-Re contents was the highest when collection was done 14 days after flowering. Conclusions: The most suitable period for ginseng berry collection was proposed to be from 14 to 21 days after flowering, as this is when the content of ginsenoside-Re, which is useful as a medicinal or cosmetic material, is still high and the ginseng root has not yet decreased in weight.

Background : Obesity, a global health problem and a chronic diseases, is associated with increased risk of developing type 2 diabetes and coronary heart diseases. A wide variety of natural remedies have been explored for their obesity treatment potential. To elucidate the anti-obesity effect of ginsenoside Rg5 : Rk1 (Rg5 : Rk1), a mixture of protopanaxadiol type ginsenosides isolated from Panax ginseng Meyer in a 3T3-L1 adipocytes. Methods and Results : In order to determinate the anti-obesity effect of Rg5 : Rk1, Oil Red O staining and triglyceride (TG) content was assessed. Furthermore, to elucidate the possible mechanism whether Rg5:Rk1 affects lipid accumulation, mRNA and protein expression analyses of adipocyte markers such as STAT3, PPARγ, CBEPα and ap2 were carried out. Rg5:Rk1 treatment showed an inhibition of lipid droplet accumulation and decrease on TG content. In addition, expression of STAT3, PPARγ, CEBPα and ap2 were decreased in dose dependent manner. Similar to these results, Rg5:Rk1 treatment reduced PPARγ and CEBPα protein expression. Conclusion : Rg5 : Rk1 treatment exhibits anti-adipogenic activity by down-regulation of the STAT3PPARγ/CEBPα pathway in 3T3-L1 adipocyte cell line.

Background : Nowadays obesity has increased dramatically in developed countries which lead various metabolic disorders such as type 2 diabetes, hypertension, cancer, and the risk of stroke. Thus, looking forward a new chemical entity from natural sources which have a strong potential of anti-lipid regulation activity. Recently, protein based nanomedicine offers a new approach to treat a number of diseases including metabolic disorder such as obesity. In this study we evaluated the anti-lipid regulation effect of nano-carrier Bovine serum albumin and Mesoporous silica (MSNPs) conjugated ginsenoside F1 in 3T3-L1adipocytes and HepG2 hepatocytes. Methods and Results : BSA incorporated drugs has been shown to protect the drug degredation as well as improvement bioavalilability. To assess the anti- adipogenic effect of BSA-F1 and MSNPs-F1 cell viability was investigated in different time point of cell cycle growth and the intracellular lipid accumulation was evaluated in mature adipocytes and hepatocytes by Oil red staining assay. In addition, transcriptional gene regulation was quantified by the real time PCR for targeting adipogenic genes such as PPARγ, STAT3, CEBPα, ap2 and aP2 as well as hepatogenic genes such as ACC, AMPK, FAS and PPARα. Moreover, protein expression was evaluated by immunoblotting assay. Conclusion : Altogether, the above results were confirmed that BSA-F1 at dose 25 μM exhibited the anti-adipogenesis effect by downregulating the major transcriptional factors PPAR γ/STAT3 in signalling in 3T3-L1 mature adipocytes and upregulated the fatty acid oxidative AMPK signaling in fatty acid induced HepG2 hepatocytes.

Background : Korean mountain ginseng adventitious roots culture extract fermentation product (KGEF) is increased the content of low molecular weight ginsenoside Rk1 and Rg5 by purifying, steaming, and fermentation of the wild ginseng adventitious roots culture. In Ministry of Food and Drug Safety, the analysis method of low molecular weight ginsenoside (Rk1, Rg5, Rh2, compound K, etc.) has not been proven, therefore we conducted validation to confirm the suitability of the qualitative and quantitative analysis method for Rk1 and Rg5. Methods and Results : Quantitative analysis was performed at a maximum absorption wavelength of 203 ㎚ (specificity). It was confirmed that the retention time of each peak of Rk1 and Rg5 was separated by chromatogram. The separation degree of Rk1 and Rg5 was 2.15 more as 1.5 a result of calculation according to the formula to evaluate the separation limit. (accuracy). The recovery rate was 101.5% of Rk1 and 103.9% of Rg5 in KGEF. (repeatability). The area value of ginsenoside Rk1 and Rg5 showed high reproducibility with relative standard deviation Rk1 0.86% and Rg5 0.68%. Retention time was also reproducible with relative standard deviation Rk1 of 0.054% and Rg5 of 0.09%. (linearity). The correlation coefficients were 0.999 of Rk1 and 0.999 of Rg5. The reproducibility of retention time in linearity was also high, with relative standard deviation Rk1 0.0017% and Rg5 0.0017% (limit of quantification, limit of detection). The quantitative limit of Rk1 was 53.73 ㎍/㎖ and the detection limit was 17.73 ㎍/㎖ and the detection limit of Rg5 was 259.03 ㎍/㎖ and detection limit was 85.48 ㎍/㎖. Conclusion : In this study, we validated ginsenoside Rk1 and Rg5 for identification and content testing. It will be enables to verify physicochemical differentiation and analytical methods, and to be a research-based data of low molecular weight ginsenosides.

Background: Hot steaming is known to be effective in improving the biological activities of plant extracts by breaking down useful compounds to low molecular weight ones. Methods and Results: This study aimed to develop an optimal extraction and steam processing method for enhancing the low molecular ginsenoside contents of the adventitious roots culture of wild mountain ginseng. The total ginsenoside was optimally extracted when 70% EtOH was used at 50℃, whereas low molecule ginsenoside such as Rg2, Rh1, Rh4 and Rk1 could be extracted using 70% EtOH at 70℃. The adventitious roots culture of wild mountain ginseng is known to contain four major ginsenosides, i.e., Rb2, Rb1, Rg1 and Rd, however new ginsenosides Rg6, Rh4, Rg3, Rk1 and Rg5 were new abundantly obtaind after steam processing method was applied. The contents of total ginsenosides were the highest when thermal steam processing was conducted at 120℃ for 120 min. Unlike ginsenosides such as Rg1, Re, Rb1, Rc, Rb2, and Rh1, which decreased after steam processing, Rg3, Rk1, and Rg5 increased after thermal processing. Steam processing significanltly reduced the content of Rb1, increased that of Rg6 by about ten times than that in the adventitious roots culture of wild mountain ginseng. Conclusions: Our study showed that the optimal extraction and steam processing method increased the content of total ginsenosides and allowed the extraction of minor ginsenosides from major ones.

Background: In Korea, 6-year-old ginseng root is economically more important than 4 or 5-year-old roots. In general, the root age is determined by counting the number of stem vestiges. However, this method does not accurately estimate ginseng root age. Methods and Results: In this study, the stem vestige counting method was used to survey a total of 18,395 fresh ginsengs cultured in 2014, and 2015, to determine the accuracy of this method. The proportion of 6-year-old roots, with more than four stem vestiges, was 46.1% in 2014. For the cultivar Chunpoong cultivated in Eumseong and Goesan countries in 2015, the proportion of more than four stem vestiges was 55.9%, and 43.5%, respectively. The proportion of more than four stem vestiges for the Gumpoong cultivated in Eumseong and Yangpyeong countries was 67.0%, and 35.1%, respectively, whereas that for the cultivar Yunpoong was 36.0% and 61.0%, respectively. Moreover, it was confirmed that differences in the levels of Rg1 will enable root age determination. Conclusions: Root age determination by the stem vestige test was found to differ depending on the environmental and cultivation conditions. To determine the age of ginseng roots, a comprehensive method, such as counting stem vestiges and evaluating differences in ginsenoside levels, should be applied.

Background : This study aimed to investigate the effect of elevated temperature by climate change on growth and ginsenoside contents of Korean ginseng, which requires low temperature during growth period. Methods and Results : Yunpoong was transplanted in temperature gradient chamber (TGC) for high temperature treatment and then growth characteristics and ginsenoside contents were investigated by growth period. Stem length, petiole length, leaf length and leaf width were increased as the temperature was higher than Ambient Temperature (Amb), but stalk diameter was decreased and small petiole length, number of petiole and leaf were no significant difference. Root growth, whereas, including length diameter and weight were decreased as the temperature was higher than Amb. Compared with Amb, root length, diameter and weight were decreased by 19.6%, 22.4% and 41.5% at Amb+6, respectively. The higher the temperatures are associated with the more ginsenoside contents. Compared to Amb, total ginsenoside contents were increased by 68% and 78% at Amb+4℃ and Amb+6℃, respectively. Almost all contents of individual ginsenoside, with the exception of Rg1, made a significant difference. Conclusion : When temperatures rises during growth period, the growth of ginseng was decreased but, ginsenoside contents were increased.