To investigate the modifying effect of Kwao Kreu, Pueraria mirifica (PM), we performed two kind of studies which are the non-surgical medium-term carcinogenicity study and the modulation of gap functional intercellular communication study. The first study, a non-surgical medium-term carcinogenicity bioassay was done to investigate the modifying effect of Kwao Kent, Pueraria mirifica (PM), a rejuvenating folk medicine from Thailand, on the male F344 rat liver. Specific pathogen free, male 6-week-old F344 rats were divided into ten groups. To induce hepatocarcinogenesis, those in all groups were given a single i.p. injection of DEN (200 mg/kg) and were received two i.p. injection of DGA (300 mg/kg) at the ends of weeks 2 and 5. Rats of group 3-6 were given sodium phenobarbital (PB 0.05 % in drink). A diet containing 10 mg/kg PM was given to group 2 during the post-initiation phase and to groups 4 and 5 during promotion and initiation phase, respectively. Group 6 was given the experimental diet alone throughout the experiment (8 weeks). Rats of group 7, 8, 9 and 10 were fed 1000 mg/kg PM in the same manner as group 2, 4, 5 and 6. All animals were sacrificed at 8 weeks after DEN administration. Result of the iimmunohistochemical staining of the glutathione S-transferase placental form (GST-p) indicated that the numbers and areas of the preneoplastic leisions were not significantly changed in all PM treatment group comparing to control group. A.Iso the numbers and areas of GST-p positive foci among group 7, 8, 9 and 10 were not significantly changed in comparing to control group. To study the effect of PM on the modulation of gap functional intercellular communication, the present study was performed scrape-loading dye transfer (SL/DT) assay in human keratinocytes. The results showed that PM could not modulate GJIC. These results indicate that Pueraria mirifzca may have no carcinogenic effects on experimental hepatocarcinogenesis in rats and gap functional intercellular communication in human keratinocyte.

Every yaar approximately 12,000 people die from severe burns and thermal injury. Distinguishing a minor burn from a more serious burn involves determining the degree of damage to the tissues of the body. The major lethal problems are massive fluid losses and microbial infections. Xanthan gum and algin are known as natural polymers. we prepared the official burn ointment which were made by xanthan gum and algin. This burn ointment was covered on the skin wound of artificial burned and their effect of healing were investigated by the evaluation of histological and hematological change as a function of time. The result of rats test showed that burn ointments made from xanthan gum and algin were more effective in formation of the new tissue and reduction of inflammation than the controls. From the results obtained in this study, natural polymer ointment which were prepared from xanthan gum and algin were found to satisfy most of the existing ointment substitute.