Pituitary-specific transcription factor (PIT1) 유전자는 동물의 성장을 조절하고 근육 형성에 관여하는 유전자로서 최근에는 단일염기다형성 변이가 한우를 비롯한 동물에서 관찰되었으며, 한우의 경제 형질과 연관성이 보고되었다. 본 연구는 PIT1 유전자의 단일염기다형성 변이가 한우에서 성장 인자에 미치는 영향과 경제 형질에 대한 유전자형간 육종가와의 상관성에 대해 알아보고자 하였다. 도체 성적을 보유하고 있는 한우 후보종모

본 연구는 후보 유전자의 경제형질에 미치는 염기 변이 효과를 검증하기 위해 국내에서 사육된 듀록 품종 96두와 한국 재래 돼지 86두를 활용하였다. 검증에 활용된 4개의 후보 유전자는 MC4R, PA-KAG3, FABP3 그리고 ESR 유전자였다. 각 후보 유전자들의 유전자형 분석 결과 두 집단 간에 유전적 특성의 차이가 분명히 나타나고 있음을 확인하였다. MC4R 유전자의 A 대립 유전자는 두 집단 모두에서 성장 형질과의 유의한 연관성이 검출되었고,

This research was performed with the subject of 111 offices in a chemical plant of Korea. Airborne concentration level of carbon dioxide, carbon monoxide, formaldehyde and total suspended particulates in the office rooms were measured along with temperature and relative humidity. Simultaneously, general characteristics and subjective health symptoms of 500 office workers were investigated through a questionnaire consisting of the five point weighting method : 0 = not at all, 1 = a little bit, 2 = moderately, 3 = quite a bit and 4 = extremely. Results showed that the mean values for temperature and relative humidity in investigated office rooms were 22.2℃ and 34.3%, respectively. The concentrations of formaldehyde, PM10, carbon dioxide and carbon monoxide were 0.17 ppb, 28.8 ㎍/㎥, 0.95 ppm and 424 ppm, respectively. 56.4% of all the workers investigated replied with a sense of fatigue or weariness (mean=2.23), drowsiness or languidness (mean=2.22), sneezing (mean=2.11) and ophthalmia oreye fatigue (mean=2.07). Female workers, non-smokers, non-drinkers, workers who do shift more than nine hours a day, and workers in poor health experienced more subjective symptoms than other workers (p<0.05). Subjective symptoms of office workers were significantly associated with the following items of office characteristics: the purchase date of office equipment, the change of office furniture, the use of paint, smoking in the office, ventilation condition, the use of an individual air-conditioner, the use of a fax machine, the degree of office repair, the proportion of workers per office, the degree of satisfaction in office environment, and the operation hours of the air-conditioner. Also, subjective symptoms were positively correlated with indoor environmental factors such as relative humidity, carbon dioxide level, level of PM10, and formaldehyde (p<0.05). In conclusion, office characteristics and air quality in a chemical plant affected subjective health symptoms of office workers. Thus, in order to improve the health of workers, to enhance work efficiency, and to establish a better office environment, air quality control in office rooms by optimal ventilation, adequately occupied number of workers in one office, and maintenance of office equipment should be fulfilled thoroughly. keywords：Air quality, Subjective health symptom, Formaldehyde, PM10, Carbon monoxide, Carbon dioxide

ISO 9000 is a management standard that provides customers with assurance thu their registered suppliers have a consistent quality system to which they adhere. this paper draws on four sources to show how ISO 9000 can lead to performance improvement. 1)theories of induces innovation and improvisation 2) the literature on ISO 9000 3) a case study of a telecom company. we find that the extent to which ISO 9000 is associated with performance improvement depends on the level of its assimilation, and the degree to which an organization goes beyond the minimal requirements of the standard.

Osteoporosis is characterized by low bone mass and the microarchitectural deterioration of bone tissue with a consequent increase in bone fragility and susceptibility to fracture. It has been suggested that speed of sound (SOS) and broadband ultrasound attenuation (BUA) of quantitative ultrasound sonography (QUS) may provide information about not only bone density but also the microarchitecture and elastic properties of bone. Physical inactivity reduced mechanical usage and it made process to the bone changes. This study aimed to association between the physical activity and the QUS parameters in 1305 (593 men, 712 women) aged 20 years over in a rural population. Two QUS parameters, BUA (p=.23) and SOS(p=.73) were measured at the right calcaneus of postmenopausal women, no significant associations were observed between sports index and SOS and BUA. These results suggest that work, non-sports leisure physical activity (p<.01) have a significant influence on QUS parameters in a rural population. Physical activity are meaningful predictor of QUS parameters of the calcaneus in a rural population.

Matrix metalloproteinases(MMPs) are involved in the degradation of extracellular matrix, which is re lated to infiltrative growth and metastasis of tumor and are regulated by tisslle inhibitor of metalloproteinases(TIMPs) or cell adhesion molecllles such as E-cadherin and epidermal growth factor receptor (EGFR). The aim of this study is to evaluate the relationship between MMP-2, MMP-9 expressions and clinico-pathologic factors, 끼MP-1 ， TIMP-2, EGFR and E-cadherin expressions. lmmunohistochemical stains were perfom1ed on 55 cases of squamous cell carcinoma of the ordl cavity and the results were as follows. MMP-2 and MMP-9 expressions were noted in 30(54.5%) and 22(40.0%) of 55 cases, TIMP-1 and πMP-2 in 21(38.2%) and 33(60.0%), and E-cadherin and EGFR expressions in 35(63.6%) and 26(47.3%) of 55 cases, respectively. MMP-2 expression rdte was slightly higher 띠 cases without recurrence, and 끼MP- 2 expression rate was slightly higher in cases showing more inftltrative growth pattem. 까1e expression rate of EGFR was higher in cases with well differentiation(p=0.OO47), but no posi디ve relationship between the expression rate of Ecadherin and histologic grade was found. Cases with positive reaction for MMP-9 showed an increasing tendency of nega디ve reaction for TIMP-1. π1e expression rate of MMP-2 was higher in cases with positive reaction for E-cadherin and EGFR with no statistical significance. 까1e expression rate of MMP-9 was significantly higher in cases with positive reaction for E-cadherin(p=0.022l). These results suggest that MMP-2, MMP-9, TIMP-1 and TIMP-2 expressions are involved in the development of oral squamous cell carcinomas, but MMP-2, MMP-9, 끼MP-1 ， and 끼MP-2 expressions might not seem to be a useful prognostic factors because there were no significant relationship between clinicopathologic parameters. EGFR expression showed positive correlation with low histologic grade, so EGFR expression could be regarded as a good prognostic factor. In the progression of sqllamous cell

MMPs are catalytic enzymes involved in the degradation of extracellular marix, and associated with invasive growth and metastasis of malignant tumors along with angiogenesis. This study was to evaluate the prognostic significance of VEGF expression and microvessel density(MVD) and the relationship between VEGF expression, MVD and MMPs expression in oral squamous cell carcinomas(OSCC). The materials were from 52 cases of OSCC during a period from 1991 to 2001. Clinicopathologic parameters such as clinical stage, recurrence, histologic grade and invasion pattern were evaluated. Immunohistochemical staining for MMP-2, MMP-9, VEGF and CD34 were performed and statistical analyses between clinicopathologic parameters and VEGF expression and MVD were done. MMP-2, MMP-9 and VEGF expressions were noted in 30(57.7%), 21(40.1%) and 38(73.1%) of 52 cases, respectively. MVD was measurable in 35 cases, and cases with increased MVD more than average were 16(45.7%) of 35 cases. There was no significant relationship between clinicopathologic parameters and VEGF expression or MVD in OSCC, which suggests that VEGF expression and MVD can not be regarded as reliable prognostic factors. Cases with less infiltrative growth pattern showed a tendency of increased MVD, which can explain the possibility that neovascularization might be an early event of tumor invasion. Lack of significant relationship between MMPs, VEGF expressions and MVD might be due to limited number of cases, and positive correlation between MMP-2 and VEGF expression suggests that both factors might be involved in the process of angiogenesis in OSCC.

미토콘드리아는 세포내의 에너지대사에서 중요한 역할을 수행하는 세포내 소기관이며, 자체의 유전물질이 모계를 통해 유전되는 특징을 가지고 있다. 포유류 초기 배아의 발생과정에서 미토콘드리아의 역할과 기능에 대한 연구는 미진한 상태이다. 본 연구에서는 생쥐 초기 배아의 발생과정에서 관찰할 수 있는 미토콘드리아 미세구조의 변화 양상을 살펴보고, 이와 초기 배아의 발생 능력과의 관련성을 밝혀보고자 하였다. 과배란 유도된 ICR 생쥐로부터 배란된 난자와 2-세포기 배아를 수획하여 76 배양액으로 포배기까지 체외배양하면서, 각각의 발생단계에 따라 시료를 수획하였다. 미토콘드리아 미세구조의 변화는 일반적인 투과전자현미경방법(TEM)을 이용하여 관찰하였다. 미토콘드리아의 미세구조는 배란 난자에서 4-세포기 배아까지는 구형이고 크리스타가 발달하지 않은 원시형태였지만, 포배기로 발달함에 따라 크리스타가 발달된 막대형의 전형적인 미토콘드리아로 분화됨이 관찰되었다. 체외배양 중에 발생이 지연되거나 정지된 배아에서 관찰한 미토콘드리아의 미세구조는 공포화 (vacuolization), 크리스타 발달 지연, 손상된 미토콘드리아의 세포막 등과 같은 비정상적인 변형을 관찰할 수 있었다. 또한, 극체에 존재하는 미토콘드리아의 미세구조는 정상적인 핵내의 유전자와의 상호작용이 없어 미분화 상태로 포배기까지 유지되는 것을 관찰하였다. 이상의 결과를 통해 미토콘드리아의 정상적인 분화 과정이 초기 배아의 발생능력과 관련되어 있음을 알 수 있었으며, 포유류 초기 배아의 체외배양시스템을 개선하는데 미토콘드리아 미세구조의 관찰과 변화에 대한 고려가 있어야 될 것으로 생각된다. buffer A 용액으로 세척하여 유리 petri dish의 바닥에 부착된 macrophage만을 cell scraper로 분리하였다. 분리한 macrophage는 0.5-1 cells/가 되게 조정하여, IL-I 을 0.001, 0.01, 0.1 또한 1 ng/를 첨가하여 농도에 따른 효과를 조사하였고, 각각 24, 48, 72, 96 또한 120시간을 배양하여 시간에 의한 효과도 실시하였다. 각 배치구에서 얻어진 배양액은 TGF-를 조사하기 전까지 -2에 동결 보존하였다. TGF-의 측정은 TGF- kit(promega, USA)를 이용하여 실시하였으며, 통계학적 분석은 Anova test를 Statview program을 이용하여 분석하였다. 시험의 결과 대조구에 비해 IL-I 첨가구는 2-3배의 TGF-생산을 보였으며, 배양시간에 따른 생산은 시간이 지남에 따라 약간 상승하는 경향을 보였으나, 유의적인 차이를 보이지는 않았다. 또한 IL-I의 농도에 따른 생산의 변화는 IL-I의 농도에 따라 약간의 차이를 보였고 유의적인 차이는 인정되지 않았다. 임신 및 비임신의 경우 임신우의 비장 macrophage가 비임신보다는 약간 상승하는 거스로 나타났다. 이상의 결과로 볼 때 IL-I 는

Objectives: The purpose of this survey research was to Investigate how well the national occupational therapy certification examination was reflecting the current occupational therapy practice. Method: The researchers developed a survey with input from 159 occupational therapists out of 230. The content of the survey consisted of four areas which were demographic information, the relationship between the current national occupational therapy certification examination, the tasks that occupational therapists perform in the clinical settings, and the knowledge bases that occupational therapists need to know to perform the tasks. Results: The findings can be summarized as follows; 1) All the current examination subjects except for ADLs (activities of daily living) had low relationship with clinical occupational therapy practice; 2) 84% of the respondents agreed on that the current examination needed to be revised based on the tasks that occupational therapists actually do in the clinical settings; 3) the new subjects proposed for the national occupational therapy certification examination showed high relationship with the clinical occupational therapy practice; 4) the new subjects were composed of occupational therapy evaluation, treatment plan, intervention, re-evaluation, management, and professionalism. Conclusions: The findings are expected to be useful for revising the content of the current national occupational therapy certification examination and developing occupational therapy curriculum of the new educational programs.

This study was performed to assess the effect of eating behaviors and health-related variables on overall dietary quality. Ninety-four(male 21, female 73) elderly who were over 60 residing in middle income areas in Cheongju city participated. Information on general characteristics of the elderly, health-related life style, regularity of meal, meal balance and desirable eating habits were obtained by interview based on questionnare. Dietary nutrient intake data were obtained through the 24 hour recall method. The mean age and BMI of the subjects were 73.3 years old and 23.3(male 21.8 female 23.7) respectively. The proportions of underweight and hypertension were 19.2% and 36.2%. Most nutrients except vitamin B2 and calcium were consumed over 75% of the RDA. The Mean Adequacy of Ratio(MAR) of nutrient intake was 0.64(male 0.72, female 0.62). The average score of regularity of meal, meal balance, and desirable eating habits was 14.4 out of a possible 16, 13.7 out of a possible 24 and 5.5 out of a possible 16 points respectively. Male than female, older subjects than young subjects, and those living with their spouses than with other family or living alone had better scores in eating behaviors. Smoking, chewing ability and eating alone vs eating with company affected overall of regulality of meal and meal balance(p〈0.05). Positive correlation (p〈0.05) was also dietary quality. There was a positive correlation between the mean adequacy ratio, score observed between scores in regularity of meal and meal balance. Therefore, the elderly should be encouraged to eat a variety of food, maintain good dental health, keep regularity of meal and have meals with company to help improve overall dietary quality and eventually achieve optimal nutritional status.

느티나무에서 외줄면층에 의해 형성되는 충영의 생육을 조사하고, 유리 아미노산의 조성과 함량을 면충의 생활사에 따라 잎, 충영 및 면충체액에서 분석하여 다음과 같은 결론을 얻었다. 충영의 형성위치는 잎의 중간분위 또는 기부 쪽에서 엽맥을 따라 엽병쪽으로 진행되었으며, 조사된 잎의 90% 이상이 1개의 충영만을 형성하였다. 충영성숙은 면충거주자가 유시성충으로 이탈된 후에도 폭과 길이에서 증가하고 있는데 이는 충영이 면충의 먹이 또는 서식처로서 뿐만 아니라

눈의 광학상수값과 상호연관성을 분석하기 위하여 건강하고 안질환이 없는 18-32 세 사이의 131 명올 대상으로 자각식 굴절검사， 자동각막곡률측정법， 각막두께측정， Orbscan Topography 및 A -scan ultrasonography를 실시하였다. 남성이 여성보다 각막전후면 의 곡률반경， 각막두께， 전방깊이， 유리체깊이 및 안축장이 더 길었으며， 수정체의 두 께와 동가굴절력은 여성이 남성보다 더 높았다. 수정체의 둥가굴절력은 원시안이 근 시안보다 높았으나， 각막의 둥가굴절력은 굴절이상의 유형 사이에서 유의한 차이가 없었다 등가구면 굴절이상도와 AL/CR 비 사이의 상관관계가 가장 높았다. 각막전면의 곡 률반경과 유리체깊이 및 안축장 사이에는 양의 상관관계가 있었고， 수정체두께와 전 방깊이， 유리체깊이， 안축장 및 AL/CR 비 사이에는 음의 상관관계가 있었다. 눈의 굴 절상태는 각막전면의 곡률반경보다는 안축장과 더 높은 상관관계가 있었다. 근시안에 서 전방깊이와 유리체깊이가 가장 길였고， AL/CR비가 더 높았다. 눈의 여러 가지 광 학상수의 평균값은 다른 인종에 대한 선행 연구결과와 차이가 있었지만 상호연관성 은 비슷하였다.

There is an opinion that Korean Buddhist Temples located in mountains(山地寺刹) should be built since the Secret Buddhism was introduced and the Zen Buddhism was widely distributed over But, Korean Buddhist temples were already in the mountains before the introduction of Zen Bluddhism. Moreover, Zen Buddhist temples were actually not located at mountainous region, but located at plain area in mountains. Thus it is necessary to modify the idea of that the location of temples were only under the influence of Secret Buddhism. The purpose of this study is to analyze the relation between the location of the Korean Buddhist Temples and transportation. The results of this study are as follows; Based on the legends originated from the buddhist temples, the traditional maps, and the political situations, it has been disclosed that the locations of Korean Buddhist Temples have the relations with the traffic roads. Therefore politically, militarily, and socially, the Korean Buddhist Temples have played important roles since the Three Kingdoms. The Buddhist temples need lodging facilities called Won-Woo for the ordinary people, due to change of the characteristics of the Buddhism in Korea from politically to socially. But the lodging facilities stood apart from main region for the ascetic exercise of the Buddhist priests.

치악산 편마암복합체에서 방사성원소를 포함하고 있는 광물을 파악하고, 주변 지하수에 포함되어 있 는 방사선원소(우라늄)와의 연관성을 확인하고자 암석학적 및 광물 화학 분석을 수행하였다. 현미경 및 전자 현미경 분석 결과, 주 구성광물은 사장석, 흑운모, 석영, 알칼리장석, 녹니석 그리고 방해석이며, 부수광물은 스펜, 갈렴석, 인회석, 저어콘, 토라이트, 티탄철석, 황철석 그리고 방연석 등 총 14종을 확인하였다. 토라이트 의 경우 거정의 갈렴석 내 ~1 mm의 크기로 소량 관찰된다. 희토류 원소를 많이 포함하고 있는 갈렴석은 각 기 다른 3 가지 산출양상을 보인다. EPMA 분석 결과, 거정의 갈렴석에서는 TiO2~1.70 wt.%, Ce2O3~11.86 wt.%, FeO~13.31 wt.%, MgO~0.90 wt.% 그리고 ThO2 ~1.06 wt.% 원소들의 함량이 높게 나타나며, Al2O3 17.35 ± 2.15 wt.% (n = 7), CaO 12.13 ± 1.81 wt.% (n = 7) 평균 함량이 가장 낮은 값을 보인다. 티탄철석을 둘 러싸고 있는 스펜 집합체의 가장자리에 존재하는 갈렴석은 Al2O3~24.00 wt.%, Nd2O3~5.10 wt.%, Sm2O3 ~0.66 wt.%, Dy2O3~0.86 wt.% 그리고 Y2O3~1.38 wt.% 원소들의 함량이 높게 나타나며, TiO2 0.35 ± 0.21 wt.% (n = 11), Ce2O3 5.25 ± 1.03 wt.% (n = 11), FeO 9.84 ± 0.26 wt.% (n = 11), MgO 0.12 ± 0.05 wt.% (n = 11) 그리고 La2O3 1.49 ± 0.29 wt.% (n = 11) 등과 같이 평균 함량이 가장 낮은 값을 보인다. 모암의 기질부에서 관찰되는 갈렴석의 화학성분은 앞서 설명한 갈렴석의 중간 정도의 값을 가진다. 연구대상인 치악산 편마암복 합체 내 미그마타이트질 편마암에는 주목할 만큼의 우라늄 함량을 가지는 광물이 발견되지는 않았다. 따라서 지하수에서 나타나는 우라늄의 기원과 주변 지질과의 연관성을 명확하게 밝혀내지는 못했다. 하지만 방사성 원소인 토륨 원소 및 희토류 원소를 다량 포함하는 갈렴석이 풍부하게 존재하는 것이 이번 연구결과로 확인 되었다.