The simultaneous analysis of multi-residual pesticides was developed using a gas chromatography (GC) method. In this study, a simple and reliable methodology was improved to detect 154 kinds of pesticides in ginseng extract sample by using a liquid-liquid extraction procedure, open column chromagraphy and chromatographic analysis by GC electron capture detector (ECD) and GC nitrogen-phosphorus detector (NPD). The 154 kinds of pesticides were classified in 4 groups according to the chemical structure. The extraction of pesticides was experimented with 70% acetone and dichloromethane/petroleum ether in order, and cleaned up via open column chromatography (3×30㎝) packed with florisil (30g, 130℃, 12hrs). The final extract was concentrated in a rotator evaporator at 40℃ until dryness. Then the residue was redissolved to 2㎖ with acetone, and analyzed by GC-ECD and GC-NPD. The applied concentration of pesticides was over 1~10㎍/㎖. The recovery tests were ranged from 70.7% to 115.2% with standard deviations between 0.3 and 5.7% of the standard spiked to the ginseng extract sample (Group Ⅰ~Ⅳ). The limit of detection (LOD) ranged from 0.001 to 0.099㎍/㎖ (Group Ⅰ~Ⅳ). The 9 kinds of pesticides were not detected. The developed method was applied satisfactory to the determination of the 154 kinds of pesticides in the ginseng extract with good reproducibility and accuracy.

Quinclorac, bentazone, 2,4-D, bensulfuron-methyl, dymuron, capropamide, pencycuron 및 ethofenprox를 동시에 분석할 수 있는 전처리 방법과 0.1% 인산 및 아세토니트릴을 이동상으로하고 225 nm를 측정파장으로 사용한 RP-HPLC(reverse phase-high performance liquid chromatography)법을 개발하였다. 동 농약성분들의 검출 및 정량한계는 각각 0.12〜0.84 ppm 및 0.34〜1.20 ppm이었고, 회수율은 78〜96%이었다. 본 방법을 통하여 시료 전처리에서 분석까지 기존 13시간이 소요된 총 분석시간을 3시간 이내로 줄일 수 있었다.

강원도 홍천 유기농 재배단지 내에서 재배한 5종의 농산물 즉, 케일, 신선초, 셀러리, 상추 및 파와 가락동 시장에서 구입한 일반재배 야채를 시료로 하여 잔류농약의 성분함량을 측정하였다. 5종의 야채에서 잔류농약 성분 함량을 측정해본 결과, 일반재배 야채의 경우는 각각 93.5, 57.7, 112.4, 76.5, 65.2 ppm이 잔류하여 규격기준 대비 75.35, 70.68, 78.49, 70.49％의 잔류율을 나타내었다. 상대적으로 유기농 야

Pesticides were extracted from samples with 70% acetone and methylene chloride in order, and then cleaned up via open-column chromatography apparatus packed with florisil, and finally analyzed simultaneously the organophosphorus pesticides using GC/NPD. Ultra-2 and Ultra-1 fused silica capillary columns were used to separate and identify the products. Recovery of most analytes from soybean sample, taken from pesticide residues well, was greater than(80%) for all except(6) analytes. This method can simultaneously determine multiple pesticides with a high degree of accuracy and precision.

본 실험은 1998년 1월부터 2001년 12월가지 4년간 경기도와 인천지역에 납품된 군납 농산물 17종(배추, 무, 오이, 감자, 사과, 양파, 파, 양배추, 고추, 당근, 상추, 호박, 마늘, 미나리, 시금치, 쑥갓, 부추)을 대상으로 유기염소계 농약 10종 (BHC, captafol, captan, chlorobenzilate, chlorothalonil, DDT, dicofol, endosulfan, folpet, tetradifon)을

To study the residue of organophosphorus pesticides in the ocean environment on the Cheju island, EPN[O-ethyl-O-4-nitrophenyl phenylphosphonothioate] and monocrotophos [Dimethyl-1-methyl-2-methy] carbamoylvinylphosphate] of organophosphorus pesticides are surveyed on coastal environment of Cheju city and Wimi in May and October 1996. The qualified limit detection of EPN and monocrotophos are 0.005ng/mL and 0.006ng/mL., in the water by GC-FPD, respectively. The qualified limit detection of EPN and monocrotophos are 0.010ng/g and 0.012ng/g in the solid such as sediment and sea organism by GC-FPD, respectively. EPN and monocrotophos are not detected in seawater and sediment. Moreover EPN and monocrotophos are neither detected in seaweed cava (Ecklonia cava), Agar (Gelidium amansii), turban sell(Batillus cornutus) and sea urchin(Anthocidaris Crassispina). EPN, monocrotophos used in the farm on the Cheju island are not residued in the coastal environment in the Cheju island.

A method for the simultaneous analysis of 31 residual organic chloride pesticides was studied using gas chromatography. Prepared analytical samples were injected to gas chromatography (HP 5890 Series II plus) on the Ultra-2 column with ECD. The packing materials for column were changed as the following reagents ; florisil and alumina N. The residual solution was loaded to column and was eluted with elution solvents ; ether : benzene (2 : 8) solution, hexane : benzene (1:1) solution, dichloromethane, acetone, and methanol. The analytical results showed that 6 kinds of organic chlorides were not detected when florisil (first condition) was used as the column packing material. The nondetected 6 kinds of organic chlorides in the first analytical condition were detected and the recoveries of thrin-pesticides were increased, in particular, captan and captafol, but the recoveries of benzene hexachloride compounds were decreased when dichloromethane and methanol were added as elution solvents (packing material was florisil as in the first condition). The recoveries of dichlorfluanid, chlorofenvinfos, folpet, and dicofol were increased and that of aldrin was increased, but those of captan and captafol were not good when alumina N was used as the packing material. To detect simultaneously thrin-pesticides, captan, and captafol, florisil and alumina N were used as the packing materials. The elution result showed that captan and captafol were not detected. This was because the column was activated insufficiently. The analytical method was the best (31 kinds of organic chlorides in the residual pesticides were detected sharply and showed high sensitivity) when the column (packing materials were florisil and alumina N, together) was fully activated and the impurities were removed using various elution solvents.