The objective of this study was to investigate changes in drying yield, pH, water activity, microbial growth, and storage stability of bottom rounds of Hanwoo beef under four different dry-aging treatments (T1–T4) as temperature (2~4℃), relative humidity (65~86%) and dry-aging period (20~90 d). The drying yield decreased by 83.13–97.05% as the drying period increased. Among the four treatments, there were no significant differences in drying yield after 60 d of dry-aging. The total plate aerobic counts (TPC) increased by 1.07–4.39 log colony-forming units (CFU)/g as the dry-aging period increased. Of the four treatments, T4 at 40, 60, and 90 d had significantly higher TPCs than those observed for the other treatments on the same days (p<0.05). As the period of dry-aging increased, pH values increased by 5.35– 5.88 for knuckle and 5.34–5.62 for sirloin muscle, and water activity values decreased. For knuckle and sirloin muscle, the water activity values at 40 d and 60 d of T1 and T4 were significantly higher than those of the other treatments on the same days (p<0.05). The thiobarbituric reactive substances and volatile basic nitrogen values of the aged products increased as the dry-aging period increased. The results of this study showed that dry-aging conditions such as those in the T2 or T3 treatments (with a gradual increase in temperature and humidity) for less than 60 d would be best for yield, reduction of microbial growth and storage stability by dry the muscle surface quickly at the beginning period. Further research should include meat quality and economic analysis for these conditions that examines the benefits of these dry-aged products for the end processor.

본 연구는 건조숙성 조건에 따른 한우 설도육의 이화학적 및 관능적 특성 변화를 조사하고자 수행하였다. 한우 거세 1등급 설도육 54개를 구입하여 온도(2℃, 4℃), 상대습도(65%, 75%, 85%) 및 건조숙성기간(10~90일)에 따라 4종(T1~T4)의 조건으로 나누어 건조숙성하면서 도가니살 및 보섭살의 육질특성과 삼각살을 포함한 관능특성을 비교하였다. 도가니 및 보섭살의 지방함량은 4개 처리구 모두에서 숙성기간이 증가함에 따라 유의적으로 증가하였다(p<0.05). 반면에 수분함량은 건조숙성기간이 증가함에 따라 도가니육은 T2와 T4, 보섭육은 T2에서 유의적으로 감소하였다(p<0.05). 도가니 및 보섭육의 육색은 건조숙성기간이 증가됨에 따라 4개의 처리구 모두 L값(백색도), a값(적색도), b값(황색도)이 유의적으로 감소하였다. 가열감량은 도가니육의 경우 T2만 건조숙성기간이 증가할수록 감소되는 경향이었고 나머지 3개 처리구는 유의적인 차이가 없었다. 보섭육의 경우 가열감량은 처리구 4개 모두 건조숙성기간이 증가할수록 감소되는 경향이었다. 동일한 숙성일 내 처리구간 비교에서는 T2가 가열감량이 유의적으로 가장 낮았다. 전단력은 도가니육과 보섭육 모두 4개 처리구에서 건조숙성기간이 증가할수록 유의적으로 감소한 반면(p<0.05) 보수력은 유의적으로 증가하였다(p<0.05). 관능특성 변화에서 도가니육, 보섭육 및 삼각살은 건조숙성기간이 증가할수록 모든 처리구에서 연도가 증가하였다. 다즙성은 도가니육과 삼각살은 T2와 T4에서 증가하였고, 향미는 삼각살만 T2와 T4에서 증가하였다. 본 연구에서 제시된 4개의 처리구에 대한 한우 설도육의 육질 및 관능특성 분석결과 전단력, 보수력 및 연도가 모두 향상되었으며 특히 처리구 2 조건은 가열감량과 관능적 기호도에서 더 유리하게 작용한 것으로 나타났다. 따라서 한우 설도육은 온도와 습도를 단계적으로 상승시키면서 약 60일 이내로 하는 것이 육질, 맛과 경제성 면에서 적절할 것으로 생각된다.

본 연구는 체성숙이 완료된 15개월령 이상의 한우 암소로부터 초음파 측정 장치를 활용하여 조사된 등지방두께(UBF), 배장근단면적(UEMA), 근내지방도(UMS)의 형질들에 대한 유전능력을 추정하기 위한 통계분석방법을 도출하기 위한 연구로써 신체충실지수 및 월령에 대한 사전 보정의 효율성을 알아보고 유전평가를 위한 형질별 유전모수를 추정함으로써 유전능력 추정의 효율성을 향상하기 위한 방안을 도 출하고자 실시하였다. 분석에 이용된 자료는 2001년부터 전국의 1,004농가에서 사육중인 15개월령 이 상의 한우 암소 38,663두로부터 조사된 초음파 측정 육질관련 형질들에 대하여 조사된 자료를 이용하였다. 흉위 및 도축형질들에 대한 성장곡선함수식을 추정하기 위하여 농가-년-월(HYM) 및 신체충실지 수(BCS)에 대한 통계모형식을 통하여 사전보정을 실시하고 Von Bertalanfy함수식을 이용한 비선형 성장곡선 함수식을 추정하였다. 또한 도축형질들에 대하여 개체별로 조사된 관측치는 성장곡선 함수식에 대한 편차로 자료변환한 후에 유전모수를 추정한 결과, 각 형질별 유전력은 UBF에서 0.29, UEMA는 0.56 및 UMS에서 0.49의 추정치를 보였다.

This study was conducted to investigate whether dietary levels of copra and palm meals affect the growth performance, carcass characteristics, and meat properties of Hanwoo steers. Eighty-one Hanwoo steers were randomly assigned to one of three dietary groups: T1 (conventional level of palm and copra meals), T2 (20% copra meal and 15% palm meal), and T3 (30% copra meal and 20% palm meal). During the growing and early fattening periods, the average daily gains and formula feed intakes of T2 and T3 were higher than that of T1; however, those differences were not statistically significant. The dietary levels of copra and palm meals did not affect carcass weight, back fat thickness, and rib-eye areas. Marbling scores and meat quality grades were lower in T3 than in T1 and T2; however, those differences were not statistically significant. Meat color, fat color, texture, and maturity were similar among the treatment groups. In addition, dietary levels of copra and palm meals did not affect the chemical and fatty acid composition of longissimus dorsi muscles in Hanwoo steers. Thus, the present results indicate that high supplementation levels of copra and palm meals do not negatively affect the growth performance, carcass characteristics, and meat properties of Hanwoo steers.

본 연구는 2006년부터 2016년 사이에 강원도 축산기술연구소에서 출생한 한우 송아지 1,069두로부터 조사된 이유시 체중과 임신기간, 생시체중 및 포유기 일당증체량 및 이유시 체중을 이용하여 단순상관, 다중 회귀 및 경로분석 등을 통해 이유시 체중과 임신기간, 생시체중 및 포유기 일당증체량 간의 상관 관계를 규명하였다. 통계분석은 조사된 원 성적을 이용하여 실시하는 동시에 조사된 원 성적을 송아지 출생 연도-계절, 송아지의 성, 어미의 산차 및 이유일령(포유기 일당증체량과 이유시 체중에만 해당) 등과 같은 환경효과에 대해 보정한 값인 잔차를 이용해서도 실시하였다. 조사된 형질들 간의 상관계수는 모두 양수였는데, 임신기간은 생시체중과의 잔차상관계수가 +0.315로서 중정도 크기였던 반면 포유 기 일당증체량이나 이유시 체중과의 잔차상관계수는 각각 +0.039와 +0.130으로서 작거나 0에 가까운 값이었다. 이유시 체중은 포유기 일당증체량과의 잔차상관계수가 +0.949로서 생시체중과의 상관계수인 +0.323보다 컸다. 잔차를 이용한 경로분석 결과 이유시 체중의 변이에 미치는 임신기간, 생시체중 및 포유기 일당증체량의 전체 기여도는 각각 0, 0.095 및 0.892로 나타났다. 본 연구 결과, 임신기간, 생시 체중 및 포유기 일당증체량 중에서 포유기 일당증체량이 이유시 체중의 변이에 가장 큰 영향력을 미치는 형질인 것으로 나타났다.

본 연구는 경북지역 거세 한우의 출하체중에 따른 도체 특성 및 부분육 생산량을 조사하여 고기의 생 산성을 도체로부터 평가하기 위하여 실시하였다. 경북지역 거세 한우 51두를 도착체중 700kg미만, 701~750kg, 751~800kg, 801~850kg, 850kg초과 5그룹으로 나누어 가공단계별 중량변화, 도체규격, 대분할 부위 생산량 및 수율, 소분할 부위 생산량 및 수율, 부산물 생산량 및 수율을 조사하였다. 가공 단계별 중량변화는 한우의 출하체중이 증가함에 따라 온도체중, 냉도체중, 대분할육, 소분할육, 뼈량, 지방량이 증가하였다. 도체규격은 한우의 출하체중이 증가함에 따라 전장, 전사분체장, 흉장, 경폭, 흉 폭, 퇴폭, 흉위, 퇴위, 흉후가 증가하였다. 부산물 생산량은 한우의 출하체중이 증가함에 따라 머리, 앞 족, 뒷족, 가죽, 간, 소장, 배지방, 생식기, 신장, 기타지방량이 증가하였다. 도체부산물은 한우의 출하 체중이 증가함에 따라 사골, 꼬리반골, 도가니, 마구리, 잡뼈, 갈비뼈량이 증가하였다.

본 연구는 우리나라 일반 농가에서 사육중인 한우 암소 348두의 도체중, 등심단면적, 등지방두께 및 근내지방도에 대하여 EBV(Estimated Breeding Value) 및 GEBV(Genomic Estimated Breeding Value)의 정확도를 비교하였다. EBV 분석은 한우 암소 348두의 혈통정보와 농협경제지주 한우개량사 업소의 당대검정우 및 후대검정우의 혈통정보 및 표현형정보를 이용하여 혈연계수행렬(Numeric Relation Matrix, NRM)을 구축하여 BLUP(Best Linear Unbiased Prediction) 방법으로 분석하였다. GEBV 분석은 표현형정보와 유전체정보가 있는 후대검정우 3,820두를 참조집단으로 이용하여 한우 암소 348두의 SNP 50K 정보와 유전체 혈연 행렬(Genetic Relationship Matrix, GRM)을 구축하여 GBLUP(Genomic Best Linear Unbiased Prediction) 방법으로 분석하였다. 그 결과, 한우 암소 348두 에 대한 EBV 및 GEBV의 정확도 차이는 도체중, 등심단면적, 등지방두께 및 근내지방도에서 GEBV의 정확도가 각각 22.90%, 11.14%, 12.28% 및 8.69% 증가됨을 보였다. 이러한 결과는 유전체 선발 시 육종가 추정의 정확도 증가를 볼 수 있을 것으로 기대되며, 이는 유전체선발을 위한 기초자료로써 활용될 수 있을 것으로 판단된다.

The purpose of this study was to investigate the effect of dietary addition of pegmatite for Hanwoo steers on the carcass characteristics. Based upon the findings yielded by the research referenced above, the results may be summed up as follows : Oleic acid was for the most part, higher as T2 plots (45.40%) and T3 plots (44.20%) compared with Con. plots (42.50%). As well, this study has shown that the treatment plots in unsaturated fatty acid (UFA), monounsaturated fatty acid (MUFA) and polyunsaturated fatty acid (PUFA) were largely higher than that of Con. plots, and of them all, T2 plots were highest. As for the melting point of fat, T2 plots (29℃) were largely lower than that of Con. plots (31.9℃). This study has shown that the treatment plots were significant higher as the content of total essential amino acid and total amino acid and inosine monophosphate in the nucleotide compound for the Hanwoo beef was treatment plots when compared with Con. plots.

This study was conducted to investigate the effects of fermented spent mushroom substrates (F-SMS) of Flammulina velutipes on growth performance, carcass traits, and economic characteristics of Hanwoo steers. A yeast strain (Saccharomyces sp. UJ14) and Bacillus strain (Bacillus sp. UJ03) isolated from fresh spent mushroom substrates of Flammulina velutipes were used as probiotics to prepare F-SMS. Twenty-four Hanwoo steers (14 months old) were allocated to three dietary treatments via a randomized block design and were slaughtered at 30 months of age. These treatment groups included Control (TMR), T1 (TMR containing 10% of F-SMS) group, and T2 (TMR containing 30% of F-SMS). Body weight gain was not influenced by the experimental diets. DM and TDN intakes in the finishing period were significantly (p < 0.05) greater in group T1 than in other groups. CP intake was significantly (p < 0.05) greater in group T2 than in other groups during the whole experimental period. Among carcass traits, rib-eye area and back fat thickness tended to increase with F-SMS supplementation. The appearance rate (%) of a meat yield more than grade A was the highest in group T1. The net profits increased by 1.2% and 13.3% in groups T1 and T2, respectively. In conclusion, if a proper feeding program (including feed safety) can be ensured, spent mushroom substrates of Flammulina velutipes can prove to be a highly profitable feed source for Hanwoo steers.

Bropirimine, a class of antineoplastic agents, is known as one of the potent immunomodulators and is currently under clinical development for the treatment of cancer. However, the effect of bropirimine on the cow remains unknown as a therapeutics agent. In this experiment, the effect of bropirimine in the peripheral blood mononuclear cells (PBMCs) stimulated by lipopolysaccharide (LPS) or concanavalin-A (Con-A) was examined. Jugular venous blood was collected from Korean Hanwoo calves and PBMCs were isolated. It was used to study the effect of bropirimine upon stimulation with LPS or Con-A for 72 hours. The expression pro-inflammatory cytokines like Tumor Necrosis Factor α (TNF-α) and Interferon γ (IFN-γ) were confirmed. Bropirimine significantly inhibited LPS- or Con-A-induced TNF-α and Con-A-induced IFN-γ in dose-dependent manner. Furthermore, Bropirimine inhibited TNF-α and Con-A mRNA expression at the transcription level. These results clearly indicated that bropirimine inhibited LPS or Con-A stimulated up-regulation of proinflammatory cytokines in a dose-dependent manner without conspicuous cytotoxicity. The bropirimine has potential to protect cow from LPS or Con-A induced endotoxin shock, possibly through inhibition of the production of proinflammatory cytokines. It suggesting that bropirimine may be a novel therapeutic agent for the prevention of inflammatory diseases. This result revealed specific features of the immune responses depending on the bropirimine compound and would help to knowledge of bovine immunity.

Genotyping-by-sequencing (GBS) is a cost-efficient method which can be useful for SNP marker discovery in a population of interest. GBS is genome reduction sequencing method using restriction enzyme. The quality of DNA is a key factor which could have an influence in downstream analysis. However, there have not been many studies which investigated the impact of DNA degradation and the quality of the data on marker discovery. In this study, GBS data of 6 Hanwoo samples (H1~6) showing differing level of DNA degradation were compared. Re-sequencing pipeline was followed to investigate the impact of DNA degradation on marker discovery. As a result, we found that the quantity and quality of SNPs were not affected in the sample H5 and H6 with moderately degraded DNA. On the other hand, marker discovery was greatly affected in samples with severe DNA degradation (H3 and H4). The findings in this study support that GBS is a robust genotyping method towards moderate DNA degradation.

본 연구는 한우가격에 미치는 도체형질의 기여도 변화추세에 대해 조사하기 위해 실시하였다. 연구에 이용된 재료는 한우 거세우 35,247두의 도축자료를 이용하여, 자료의 구조분석과 일반성적, 분산분석 및 유의성검정, 가격에 대한 도체형질의 기여도분석을 실시하였다. 효과검정을 위해 등지방두께, 배최 장근단면적, 도체중, 근내지방도, 경락가격, 전체가격 등 6개의 형질을 이용하였고, 분석한 도축년도의 효과검정에서 조사된 모든 형질이 고도의 유의적인 차이(p<0.01)를 나타내었다. 한우가격에 미치는 도 체형질의 기여도 분석을 위해 다중회귀분석을 실시하여 각 독립변수의 준부분상관자승값(squared semi-partial correlation)을 이용하였다. 경락가격의 R2 값(결정계수)은 0.5422로 나타났고 전체가격 의 R2 값은 0.7141로 나타났다. 경락가격에 대한 준부분상관자승값 결과는 등지방두께가 0.0235, 배최 장근단면적이 0.0072, 도체중이 0.0001, 근내지방도가 0.3821로 나타났고, 전체가격에 대한 준부분상 관자승값은 등지방두께가 0.0161, 배최장근단면적이 0.0047, 도체중이 0.1691, 근내지방도가 0.2341로 나타났다. 가격에 미치는 도체형질의 기여도 변화추세는 2011년 이후 육질의 기여도가 꾸준하게 떨어지 는 추세를 보이고 있었고, 육량형질의 기여도는 높아지는 추세를 나타내고 있었다. 근내지방도의 가격 기여도가 낮아지는 것은 가격의 변화, 1등급 이상 출현율 상승, 도축물량증가로 인해 변별력이 떨어진 이유라고 보여지며 이러한 결과는 장기적인 관점을 가지고 지켜볼 필요가 있으며, 한우 산업이 경쟁력 을 갖기 위해서는 추가적인 연구가 진행되어야 할 것으로 사료된다.

본 연구는 부모의 혈통정보를 모두 아는 혈통 및 고등등록우로 등록된 한우 암소 25,516두의 선형 및 외모심사형질에 대해 유전모수를 추정하였다. 각 심사형질에 영향을 주는 것으로 판단되는 주요인을 선 정하여 다형질 개체모형에 적용하여 EM-REML 알고리즘 분석방법을 통해 각 형질에 대한 유전력과 유 전상관과 표현형상관을 추정하였다. 한우 암소의 17개 선형심사형질 및 10개 외모심사형질(종합점수 포 함)의 유전력 추정치의 범위는 0.03(유두배열)에서 0.42(체장)까지로 추정되었다. 체고, 체장, 강건성, 체심, 윤곽성, 정강이두께, 피모의 색, 엉덩이기울기, 고장, 좌골폭, 넓적다리의 두께, 유방용적, 유두길 이, 유두배열, 발굽기울기, 뒷다리 비절기울기, 뒤에서 본 뒷다리자세, 체적․균형, 자질․품위, 머리․목, 전구, 중구, 엉덩이, 넓적다리, 유기, 지제보양 및 종합점수의 유전력은 각각 0.4, 0.42, 0.27, 0.25, 0.06, 0.14, 0.22, 0.31, 0.19, 0.17, 0.29, 0.04, 0.07, 0.02, 0.11, 0.03, 0.16, 0.27, 0.08, 0.15, 0.14, 0.14, 0.19, 0.16, 0.05, 0.08 및 0.3으로 추정되었다. 한편, 다형질개체모형에 의해 추정된 17개 선형심사형질과 종합점수에 대한 유전상관추정 결과는 다음과 같다. 고장과 좌골폭간에 0.96의 가장 강 한 양(+)의 유전적 관계를 나타내었다. 반면 발굽기울기와 뒷다리 비절기울기간에는 -0.57의 가장 큰 음(-)의 유전상관을 보였다. 전체외모 형질인 체고, 체장, 간겅성 및 체심은 각 형질간에, 그리고 기타 형 질들과 강한 양(+)의 유전상관관계를 나타내었다. 특히 체고 및 체심과 고장 및 좌골폭간에 높은 양(+)의 상관관계를 나타내었으며, 체고와 엉덩이기울기간에는 0.32의 양(+)의 상관을 나타내었다.

Antioxidants have been added to cryoprotectant or in vitro culture medium for sperm to reduce the detrimental damage, such as reactive oxygen species. However, curcumin, an antioxidant, shows dual effect on the viability and progressive motility of bovine sperm exposed to hydrogen peroxide. Low concentration of curcumin increases sperm viability and progressive motility, whereas high concentration of curcumin reduces them. This study was performed to identify whether TREK-1 channel is related to low sperm viability and motility induced by high concentration of curcumin. Curcumin reduced TREK-1 channel activity in a dose-dependent manner. TREK-1 channel was expressed in sperm obtained from Korean native bull. Treatment with TREK-1 channel blockers, such as curcumin, fluoxetine, GdCl3, and spadin, significantly reduced sperm viability and motility (p < 0.05). However, TREK-1 channel activators showed different effect; linoleic acid showed an increase in sperm viability and motility, and wogonin did not affect them. These results show that TREK-1 channel is involved in the regulation of sperm viability and motility. In particular, high concentration of curcumin might reduce sperm viability and progressive motility of Korean native bull through blockage of TREK-1 channel.

This study was conducted to investigate the breeding status of farms to improve the production efficiency of Hanwoo calf. The study was conducted on 45 farms divided into two groups. This study was conducted to investigate the breeding size and breeding area of Hanwoo cows. The average age at first delivery of Hanwoo was 28.7 months. The number of artificial insemination per pregnancy was 1.45±0.32, and the number of artificial insemination days after birth was 119.8 days. Conception rates were 75.2±16.93% for small farms and 70.6±17.46% for medium sized farms and 71.4±11.03% for large farms. When we looked at farming methods, ‘the farmers using estrus observation aids’ had 10.42% higher calf production rate than the ‘unused farmers’. The farms vaccinated with IBR and BVDV for breeding cattle showed a 4.41% decrease in abortion, stillbirth and mortality. According to farming conditions, conception rate and delivery rate improved by 3.47% and 18.29%, respectively, when grazing and exercising were performed. Observation, immunization and grazing were found to be important indicators for improving calf production efficiency in Hanwoo farm. This study can be used as a research data to improve the reproductive rate of farmhouse sites through the survey on the breeding status of Hanwoo farmers.

This study was conducted to investigate the optimal artificial insemination (AI) time with diagnostic kit at ovulation time. We already applied the patent about the protein in the cow heat mucose in external reproductive tract. And we would examine the accuracy for detection of cow heat by the kit produced with the protein. Evaluation of optimal heat detection was tried two time at 12 hrs and 24 hrs after the heat. And then, AI service also performed two times with no relation to the results of heat diagnosis by heat detection kit and pregnancy rates were checked with rectal palpation on 60th day after AI. Heat diagnostic results by kit in natural heat after 12 hrs in Hanwoo cows were showed 31.3~75.0% on positive in first heat detection and 33.3~100.0% on positve in second heat detection. In the 1st positive results were significant different (p<0.05), but 2nd positive were not. The results of heat detection showed different result on regional influence and individual cow effects. The pregnancy rates of first trial of heat detection were showed 34.4~78.7% on positive and 21.3~68.8% on negative after the diagnosis by heat detection kit. And the pregnancy rates of next trial of heat detection were showed 33.3~85.7% on positive and 14.3~66.6% on negative after the heat diagnosis. Both positive results of first trial and next trial also were showed significant different (p<0.05), but negative results were not. In positive result, first trial of total pregnancy rates was higher than the next trial of pregnancy, but there showed opposite results on negative results. In conclusion, the optimal heat detection kit is suitable to ordinary Hanwoo cows and it suggested that we have to improve the kit’s accuracy by detecting the materials like proteins related optimal AI time.

Commercial applications of OPU/IVP were to produce embryos and calves from high genetic cows.The aim of this present study was to compare the number of recovered oocytes and cultured in vitro produced embryos from Ovum Pick-up (OPU). OPU derived embryo production was carried out of oocytes by ultrasonographic guided follicular aspiration and then produced in vitro produced blastocysts by IVP culture system. In result, the rate of recovered oocytes was obtained 612 (57.2%) and 451(73.7) G1+G2 grade oocytes. No difference of recovered rate (51.1~62.1%) was seen in six donor. The rate of cleavage and blastocyst development were obtained 320 (70.9%) and 78 (24.4%) that was 3.3±0.4 cleaved embryo and 0.9±0.2 blastocysts per session. Cleavage rate of OPU oocytes in No. 6 donor was 90.6%, significantly (P<0.05) higher than that in the other donors, However, blastocysts was similar (25.8~30.0%). In conclusion, limited numbers of OPU oocytes had competent development when cultured in SOF culture medium