This study was carried out to know the variation of soybean seed proteins, 11S and 7S globulins, and their amino acid compositions among different colored soybean varieties, 'Danbaegkong' (yellow), 'Pureunkong' (green) 'Jinyulkong' (brown), and 'Geoumjeongkong l' (black). Soybean seed proteins showed a wide range in molecular size, but the electrophoresis patterns of total seed protein subunits showed a similarity among different colored soybean varieties. Amino acid compositions of total seed proteins were similar for all soybean varieties tested. However, soybean varieties showed low composition rates in sulfur containing amino acids. The composition rates of cysteine and methionine in the 11S globulins were higher than those of total seed proteins and 7S globulins. Glutamic acid and glycine were higher in the 11S and 7S globulins than those of total seed proteins. However, the levels of methionine and phenylalanine are high in the 11S globulins, but those of valine and lysin are slightly lower than the 7S globulins. By using HPLC, we tried to analyse the soybean seed proteins. The 11S globulin was composed of 10 major peaks whereas the 7S globulin was composed of 4 major peaks. The composition rates of 11S related proteins have a tendency to increasing during the maturing whereas those of 7S related proteins have a tendency to decreasing. Composition rates of each peaks among different colored soybean varieties suggested that soybean seed proteins are varied, although they showed similarity in the electrophoresis patterns, and understanding of this characteristics is important for the utilization of soybeans.

Soybean varieties derived from Korea are classified into four groups on the basis of their food types such as soybeans for vegetable, sprout, sauce and paste and soybeans with colored seed coat. This study was carried out to know the differences in storage protein concentrations among these four groups. There were differences in storage protein concentrations among four groups. In 7S protein, the α '-and α -subunit concentrations did not vary among four groups, while a β -subunit concentration greatly varied. 7S protein concentration was the highest(40.6%) in soybean for sauce and paste and the lowest(37.7%) in soybean for vegetable, while 11S protein concentration was the highest (62.3%) in soybean for vegetable and the lowest (59.4%) in soybean for sauce and paste. In view of the fact that 11S protein has much higher sulfur containing amino acids than 7S protein, it was shown the soybeans for vegetable may have higher nutrition value than other groups.

콩 저장 단백질의 대부분은 globulin이며, 이중 7S와 11S가 70% 이상을 차지한다. 따라서 콩 단백질의 조성개량을 위해서는 11S/7S 비율 조정이 우선되는데, 본 연구에서는 몇가지 콩 품종을 사용하여 7S와 11S를 분리 확인하고, 이들 분획 단백질을 이용한 두부제조시험을 실시하였다. 7S와11S는 콩 분말을 탈지한 후 pH를 조정하고 원심분리를 이용함으로써 분획할 수 있었고, 전기영동상(SDS-PAGE)에서 이를 확인할 수 있었다. 공시계통들의 11S/7S 비율은 1.29∼1.38을 나타내어 계통간 차이가 없었다. 한편 콩 종실 단백질을 7S와 11S로 분획한 후 7S와 11S를 비율별로 혼합하여 두부를 제조하고 물성을 측정한 결과 11S량이 많아질수록 두부의 경도, 탄력성, 응집성 등이 높게 나타난다. 따라서 11S와 7S의 비율을 조정함으로써 여러가지 용도에 알맞는 식품개발이 가능하리라 판단된다.