The purpose of this study is to examine the activity ratios of global trunk muscles and local trunk muscles in relation to adjustments in the level of task difficulty while performing stability exercises in easily applied bridging lumbar stabilization exercise. Twenty healthy subjects performed bridging lumbar stabilization exercise while the level of task difficulty was plate was used in the same posture for all the exercises. EMG was used to examine the activity ratios of the global muscles and multifidus in relation to the level of task difficulty. Moreover, the activity ratios of the multifidus muscle, the erector spinae and the gluteus maximus muscle were measured. A one-way ANOVA with repeated measures was used, and a Bonferroni correction was conducted (=.05). When the bridging lumbar stabilization exercise were performed at different difficulty levels, the activity of the multifidus muscle, which is a local muscle, was high in all three exercises. Also, compared to low intensity and intermediate intensity exercises, high intensity exercises showed more significant differences (=.05). Among all the muscles, the multifidus showed the highest activity at intermediate intensity. Based on these results, we suggest that in the case of bridging lumbar stabilization exercise, low intensity or intermediate intensity exercises are more suitable and efficient for local muscle stabilization.

이 논문에서는 충청도지역의 주요 문화공간인 공연장을 중심으로 지방 공연장의 운영 실태와 공연장의 규모 및 시설의 형태, 년 간 행사되는 공연물의 공연 실적 등을 통해 지방문화 예술의 문제점을 제시하고 지방문화의 발전 활성화를 위한 구체적 방안을 모색하고자 한다. 충청도지역의 공연장의 형태와 시설 및 공연 실적의 비교는 최근에 신설된 공연장을 포함한 비교적 공연활동이 활발한 공연장을 중심으로 하였으나 공연예술 보다는 일반교양 행사에 주로 대관 되는 지방 공연장의 특성으로 인해 극히 적은 수의 공연 실적을 토대로 충청도지역의 공연활동을 평가하는 자체가 무리가 있다. 그러나 공연장의 공연 시설 및 공연 실적을 통하여 무용을 포함한 각종 공연물이 공연장으로 인해 발생되어질 수 있는 문제점을 발견하고 보완함으로서 무대예술의 발전과 나아가 지방문화의 발전을 꾀할 수 있는 방법을 제시하는데 도움이 될 수 있을 것으로 사료되는바 상호 비교를 감행하였다. 그러므로 본 논문은 충청도지역의 문화공간인 주요공연장을 중심으로 공연장의 운영현황 분석을 통해 충청도지역과 문화예술의 활성화를 위한 개선방안을 제시하는데 그 목적을 둔다.

This study was conducted to investigate the effects of oocyte maturational age and activation condition on in vitro development of porcine parthenogenetic embryos (parthenotes). Porcine follicular oocytes were matured in vitro for 30 to 44 hr. Maturation rate was examined during in vitro maturation (IVM) every 2 hr interval. The cdc2 kinase activity was measured at 36 and 44 hr of IVM. Some oocytes were activated at 36 or 44 hr of IVM by three different conditions; 1) single electric stimulation (1.5 kV/cm for 30 sec; ES), 2) double electric stimulations (1.5 kV/cm for 30 sec, followed by 1.0 kV/cm for 50 sec after 1 hr; ES+ES) or 3) ES+ES followed by culture in 6-dimethlyaminopurine (6-DMAP) for 4 hr (ES+ES+D), and cultured for 6～7 days. Maturation rate was significantly increased as culture period was increased to 36 hr (66.9%, p<0.05), and then gradually increased to 87.1% at 44 hr of IVM. The cdc2 kinase activity was decreased (p<0.05) with culture period prolonged from 36 hr to 44 hr. Lower blastocyst formation rate (4.3%, p<0.05) were obtained by ES in 36 hr-matured oocytes compared to other treatments (16.5 and 20.5%) in the same age and the same treatment in 44 hr-matured oocytes (15.0%). High blastocyst formation rate (23.6%) was obtained by ES+ES+D in 44 hr-matured oocytes (p<0.05). These results demonstrate that porcine oocyte activation and in vitro development of parthenotes can be affected by interactions between oocyte maturational age and activation condition.

본 연구는 동물복제 및 형질전환 동물생산 등의 연구에 기초자료를 제공하고자 재래산양의 oocyte를 단위발생을 유도하여 회수난자의 조건, 활성화방법, 배양조건 등이 단위발생란의 체외발달율에 미치는 영향을 조사하여 재래산 양의 최적의 배양조건을 확립하고자 실시하였다. 난자의 회수는 체중 15～25 Kg 전 ㆍ 후의 성숙한 미경산 재래산양에 FSH와 PMSG 를 사용하여 과배란을 유기하여 hCG 투여 후 제 35시간째에 외과적인 방법으로 in vivo (체내성숙) 난자는 난관을 관류하는 방법으로 회수하였고, in vitro (체외성숙) 난자는 난포로부터 흡입하여 난포란을 채취하여 약 22시간 체외성숙을 실시하였다. 활성화 처리는 전기자극법과 Ionomycin + 6-DMAP를 처리하여 단위발생을 유도하였다. 복제수정란의 배양은 M16, TCM-199 및 mSOF 배양액으로 6～7일 동안 체외배양을 실시하였다. 활성화를 유도하기 위하여 전기자극 및 ionomycin + 6-DMAP 처리를 하였을 때 분할율은 각각 64.1 및 76.5%로서 이들간에 차이는 없었다. 배반포기로의 발달율은 전기자극방법으로는 전혀 발달이 이루어지지 않았으나, ionomycin +6-DMAP 처리방법에서는 15.6%가 배반포로 발달하였다. In vivo 난자와 in vitro 난자를 활성화를 유도하였을 때 분할율은 86.8 및 69.0%로서 이들간에 유의적인 차이는 없었다. 4-세포기(93.9% vs 66.1%), 8-세포기(90.9% vs 37.0%) 및 상실배기(89.4% vs 23.6%)는 이들간에 유의적(P<0.05)인 차이가 있었으며, 배반포기로의 발달율은 체내성숙난자가 50.0%로서 체외성숙난자의 0.8%보다는 유의적으로 높았다. 활성화를 유도한 난자를 mSOF 배양액으로 체외배양을 실시하였을 때 분할율은 81.0%로서 TCM-199 +oviduct cell 의 64.3% 및 Ml6 배양액의 51.6%보다는 높게 나타났다. 배반포기로의 발달율은 mSOF 배양액에서는 3.4%가 발달하였으나, TCM-199+ oviduct cell 배양액과 M16 배양액에서는 전혀 발달이 이루어지지 않았다. 이상의 결과는 포유동물 난자의 단위발생의 유도 및 체외배양시 난자의 공급원, 난자의 활성화 방법 및 배양조건 등이 차후 단위발생란의 체외발생율에 크게 영향을 미칠 수 있는 근거를 제시해 준다.

The information technology(IT) development of telecommunication, along with the informationalization over the world, enables the firms to process their inner and outer operations through Electronic Data Interchange (EDI) system. And the concept of logistics has become more important to the enterprises in strategic sense because it had aggrandized itself to more comprehensive and integrative concept. Also the world shipping market is more competitive than any other market. In these environment, to maintain competitve power, shipping firms process operations by Logistics Information system(LIS), And then, reduce the working processes, working time and its costs. So the LIS has become more important in developing our national logistics system and industry. Therefore, this study purposes on empirically analyzing the activation factors of shipping LIS and the relationship these factors with logistic performances in the shipping industry. Considering environments of Shipping logistics information system, this study sets up the research model and hypothesis to examine the activation factors of shipping LIS and the relationship these factors with logistic performances. We used the field data on the 163 numbers of firms(29 overseas shipping companies and 133 forwarding Agency) to get the empirical evidence. According to the study results, The shipping LIS activation factors which the sample firms recognized as important: were top manager's support, well structured communication channel, strategic plan's connection and inner informationalization level. And IS organization ＆ human resources, IS discipline ＆ training, software development, IS organizational alignment, database management system, information standardization, competitive advantage, financial support and government support represent partially significant. Also, the current shipping LIS's main problems are expensive system cost, standardization of working process's, institution's imperfection and system's interaction defect. So these problems must be improved to activate shipping LIS.