Generally, in aphid groups, taxonomically deficient characters and high level of morphological plasticity induced by environmental factors make difficulties for species identification. To solve these problems, DNA barcoding has been widely used for rapid and reliable species identification in aphids. The subfamily Calaphidinae is the second largest group in the family Aphididae with about 400 species belong to 59 genera. But so far, no trial of DNA barcoding has been conducted for the subfamily Calaphidinae. In this study, a total of 446 Cytochrome oxidase I (COI) sequences of 76 morphospecies collected in Korea and other countries were analyzed to detect cryptic diversity. In addition, 551 sequences of 74 species from the Genbank and BOLD system were compared with our new dataset. The final dataset consisted of 998 sequences of 115 species. As a results, we propose 12 cryptic species with discussion on morphological and ecological comparisons. Our results suggest that DNA barcoding is effective for precise species identification in this group and contributes to reveal hidden diversity.

온실재배 오이에 발생하는 목화진딧물의 발생밀도를 추정하는 표본조사법을 개발하기 위하여 2개년(2013-2014년) 동안 주 전체의 잎별 발생밀도를 조사하였다. 목화진딧물의 공간분포 특성은 일반적으로 사용되는 Taylor’s power law(TPL)와 Iwao’s patchiness regression (IPR) 두 가지 방법을 이용하여 분포특성을 조사하였다. 목화진딧물의 주 전체 밀도를 대표할 수 있는 표본단위를 일정 잎 위치의 평균밀도와 주 전체의 잎당 평균밀도와의 일반선형 회귀식을 이용하여 결정하였다. 적정 표본단위는 오이 생육기에 따라 달랐는데, 총엽수가 9매 미만일 경우 2매(중위 엽과 최하위+1번째 엽), 그 이상인 경우에는 3매(위로부터 4번째, 7번째, 최하위+1번째 엽)를 조사하는 것이 적합하였다. 오이에서 목화진딧물의 공간분포 특성은 TPL과 IPR의 기울기가 모두 “1”보다 커 집중분포를 하고 있었으며, 진딧물의 평균-분산 관계를 TPL이 IPR보다 더 잘 설명하 였다. TPL의 기울기와 절편은 연차간에 차이가 없었으며, Green과 Kuno의 식을 이용하여 고정 정확도(D) 수준에서의 축차표본조사법을 개발 하였다. 목화진딧물의 축차표본조사법은 Green의 방법이 Kuno에 비해 더 효율적이었다. 목화진딧물의 일정 평균밀도를 추정하기 위해 필요한 조사 주수는 D값과 잎당 평균밀도가 낮을수록 증가하는 경향이었다. 표본조사를 중지할 수 있는 누적 진딧물 수는 D값이 낮을수록, 조사 주수가 적을수록 증가하는 경향이었다. 목화진딧물 잎당 10마리의 밀도를 추정하기 위해 필요한 조사 주수는 13주이었으며, 이 때 조사를 중지하기 위한 누적 진딧물수는 131마리이었다.

This study was conducted to determine the toxicity to adult Myzus persicae (Sulzer) and Aphis gossypii Glover of 88 plant essential oils and six experimental spray formulations containing bitter orange or marjoram oil was examined using the vapor-phase mortality and spray bioassays. Results were compared with those of two conventional insecticides deltamethrin and dichlorvos. As judged by 24 h LC50 values, bitter orange oil (0.0212 and 0.0192 mg/cm3) was the most active material, followed by marjoram, celeryseed, and cypress oils (0.0239-0.0508 and 0.0209-0.0542 mg/cm3). These essential oils were significantly less toxic than dichlorvos, respectively. These essential oils were consistently more toxic to adult M. persicae in closed versus open containers, indicating that toxicity was achieved mainly through the action of vapor. Bitter orange oil and marjoram oil applied as 1% spray provided complete mortality toward two aphid species adults. Reasonable aphid control in greenhouses can be achieved by a spray formulation containing the 1% oil as potential contact-action fumigant.

The genus of Cinara (Aphididae: Lachninae) live on the roots, trunk, branches, twigs, shoots or foliage of Coniferae and can be easily distinguished in having the very big body, apterae up to 5-6 ㎜ in length and short antennae which are shorter than half the body length. Most of species are monoecious without host alternation and attended by ants. Based on body appearance and COI gene sequence, we report Cinara cuneomaculata (Del Guercio) on Larix kaempferi, C. largirostris Zhang G. & W. Zhang on Pinus thunbergii and P. dnesiflora, C. smaragdina (Pashtshenko) on Abies holophylla and C. piceae on Juniperus rigida, for the first time in Korea.

The genus Mesocallis Matsumura, 1919 is reviewed from the Korean peninsula. Total of five species are recognized by adding two new and one new unrecorded species to current two recorded species. In this study, diagnosis and illustrations for all five species are presented.

목화진딧물(Aphis gossypii Glover) (Hemiptera: Aphididae)은 감귤에서 그을음병을 유발하여 감귤 과실에 경제적으로 피해를 주는 해충이다. 본 연구는 무가온 하우스 감귤에서 계절초기 목화진딧물이 어떠한 경로를 거쳐서 감귤나무에 정착하는지 구명하기 위하여 수행하였다. 감귤나무에서 진딧물 월동알 조사결과 가을순이 난 여름순에서 많이 발견되었는데 월동알은 대부분 조팝나무진딧물이었으며, 목화진딧물 월동알은 발견되지 않았다. 봄순에 정착하는 목화진딧물 유시형 성충은 크게 4월 하순(조기비산)과 5월 하순(지연비산)에 두 차례 관측되었다. 지연비산은 완전생활형 간모세대에서 유시형이 출현하는 시기와 일치하였다. 조기비산 시기에 황색수반트랩에서도 목화진딧물 유시형 성충이 유살되었는데 확실하지는 않으나 월동잡초에서 잔존하고 있던 불완전세대 개체군으로 추정되었다. 결과적으로 무가온 하우스 감귤에서 목화진딧물의 정착은 불완전생활형의 조기비산과 완전생활형의 지연비산으로 일어난다는 것이 확인되었다.

The subgenus Nippocallis (Matsumura, 1917) is one of the 10 subgenera of the genus Tuberculatus Mordvilko, 1894. Originally, this subgenus was regarded as an independent genus of the subtribe Myzocallidina by the distinct morphological features and the host plant association with Castanea (Fagaceae). We performed molecular and morphological analyses to determine whether genus Nippocallis belongs to Tuberculatus group. Molecular analysis of the combined data set of Cytochrome Oxidase subunit I COI, Cytochrome B CytB and Elongation Factor I-alpha EF1α indicated that Nippocallis form a sister clade of the genus Myzocallis Passerini, 1860, apart from the genus Tuberculatus. In this contexts, we propose Nippocallis as a separate genus of the subtribe Myzocallidina.

양배추가루진딧물[Brevicoryne brassicae (Linnaeus)]의 생명표를 작성하고자 다채(Brassica campestris var. narinosa)를 기주로 7개 온도 (15, 18, 21, 24, 27, 30, 33±1℃, 65±5% RH, 16L : 8D)에서 온도발육 실험을 수행하였다. 24℃에서 온도별 사망률은 어린약충기간(1 st ~ 2 nd )에서 18%였고, 노숙약충기간(3 rd ~ 4 th )에서는 0%였다. 18℃에서 어린약충의 발육기간은 8.4일이었고, 온도가 증가 할수록 발육기간은 짧 아졌으며 노숙약충은 6.7일이었다. 선형회귀분석을 이용한 전체약충의 발육영점온도는 7.8℃였고, 유효적산온도는 120.1일도였다. 성충의 수 명과 산자수는 모든 약충이 사망한 15℃와 33℃를 제외하고 5개 온도에서 조사하였다. 성충수명은 21℃에서 14.9일이었고, 24℃에서 58.5개 의 산자를 생산하였다. 생명표에서 순증가율(Ro)은 24℃에서 47.5였으며, 내적자연증가율(rm)과 기간증가율(λ)은 27℃에서 각각 0.36, 1.43이 었다. 배수기간(Dt)은 27℃에서 1.95d이었고, 평균세대기간(T)은 30℃에서 7.43d이었다.

The Ceratovacuna aphids are one of the major gall-forming aphids. Four Ceratovacuna aphids have been reported from East Asia including Taiwan, Korea, and Japan. In Korea, only one species, Ceratovacuna nekoashi (Sasaki), has been confirmed amongst the four species, and yet their colonies have been found only from the banana bundle or cats-paw shaped galls on the primary hosts, Styrax trees (Styraceae). Colonies on the secondary hosts (Gramineae) have not been seen from Korea nor confirmed from Taiwan, although they are usually seen from Japan limitedly on stiltgrass Microstegium vimineum. Recently in Korea, a colony consisting of an emigrant and first instar soldiers were found from a grass species, Seteria sp. that have not been reported as a secondary host of C. nekoashi. Identity of C. nekoashi is definite based on morphology of the first instar soldier. Genetic identity is being explored for confirmation.

Two new species of the subfamily Greenideinae, Greenidea sp. nov. and Eutricosiphum sp. nov., are newly recognized from Laos on Glochidion fagifolium Bedd. (Malpighiales: Phyllanthaceae) and Quercus sp. (Fagales: Fagaceae), respectively. They are described and illustrated in comparison with congeneric species for apterous viviparous females. Greenidea sp. nov.is mainly characterized by relative length of siphunculus and width of same segment, and Eutricosiphum sp. nov. is distinguished by the relative length of ultimate rostral segment and 2nd hind tarsal segment.

온실에서 재배되는 수입 알로에묘목에 알로에베라진딧물, Aloephagus myersi Essig, 이 채집되었다. 이 종의 원산지는 아프리카로 알려져 있고 수입된 식물을 통해 온실에 도입된 것으로 추정된다. 우리나라의 자연환경에 도입과 정착을 방지하기 위한 이들 종의 진단형질, 사진자료, 기주 및 분포정보를 제공하고자 한다.

해충저항성 유전자변형 작물은 대상 해충에 대한 방제 효과를 증진시킬 수 있는 효과적인 수단이 될 수 있지만, 생태계 내의 다른 비표적 생물에 부정적인 영향을 줄 가능성 또한 있기 때문에 상업적인 재배에 앞서 이에 대한 충분한 연구가 이루어져야 한다. 본 연구에서는 배추좀나방 내성 유전자변형 Bt (Cry1Ac1)양배추가 비표적 곤충인 복숭아혹진딧물에 미치는 영향을 알아보기 위한 기초적인 실험으로서 기주선택실험 및 성장실험을 실시하였다. 기주선택실험에서, 두 개체씩의 Bt 양배추와 모본 양배추에 각각 10마리의 유시 성충(alate virginoparae)을 접종하고, 사흘간 생식(reproduction) 기회를 제공한 후 생산한 약충의 마리수를 측정한 결과, Bt 양배추와 모본 양배추에 낳은 약충의 수가 각각 21.9±1.8와 22.5±2.2로 비슷하였다. 성장실험에서, 갓 태어난 무시 약충(apterous virginoparae)을 Bt 양배추 및 모본 양배추를 기주로 하여 키웠을 때, 성충이 될 때까지 걸린 시간은 Bt 양배추와 모본 양배추에서 각각 5.8±0.2, 5.9±0.1 일(day)로 통계적으로 유의한 차이가 없었고, 개체군 생장률(rm) 또한 0.7±0.1, 0.8±0.1로 유사하였다. 이상의 결과들로 볼때 Bt 양배추가 복숭아혹진딧물에 미치는 영향이 없거나 미미함을 알 수 있으나, 다양한 관점에서의 보완 연구가 더 필요하다.

The effect of electron beam irradiation on development and reproduction of susceptible strain (S) and imidacloprid-resistance strain (IMI-R) of Aphis gossypii were compared. Nymphs and adults of S and IMI-R strain were irradiated at target doses of 50, 100, 150, and 200 Gy. When nymphs were irradiated, emergence rate was not affected at all target dose, but number of F1 nymphs was perfectly inhibited at 150 Gy in both strains when irradiated to the adults, longevity slightly decrease at 150 Gy and above. Fecundity was strongly decreased at 100 Gy, but was not completely inhibited even at 200 Gy. Emergence rate of F1 nymph was decreased at 100 Gy and completely inhibited at 200 Gy. However, there was no significant differences on development and reproduction of S and IMI-R strain. We also conducted the comet assay immediately after irradiation and over the following 7 day periods. In addition, we performed quantitative real-time PCR on several genes.

The melon and cotton aphid Aphis gossypii Glover (Hemiptera; Aphididae) is one of the most serious pests worldwide. We surveyed insecticide susceptibility in A. gossypii field populations to 12 insecticides (6 neonicotinoids, 3 pyrethroids and 3 others) to examine resistance ratios. The levels of insecticide resistance were extremely high, especially to neonicotinoids, such as acetamiprid, clothianidin, thiacloprid and imidacloprid. To identify the neonicotinoid resistance mechanisms, we used an imidacloprid-resistant (IMI-R) strain as a model strain. IMI-R showed an extremely high resistance ratio and also cross-resistance to all the test neonicotinoids. However, there was little or no cross-resistance to the other insecticides, including sulfoxaflor. Synergist tests and enzyme activity assays suggested the absence of resistance mechanisms based on enhanced detoxification enzymes, such as cytochrome P450, esterase and glutathione S-transferase. One point mutation was found in the beta1 subunit loop D region of the nicotinic acetylcholine receptor (nAChR) of the IMI-R strain. This R81T point mutation was also found in field populations collected from 5 regions. Therefore, the R81T point mutation was identified as an important mechanism of imidacloprid resistance in A. gossypii.

The genus of Stomaphis (Aphididae: Lachninae) is stem or root feeding aphid on trees and can easily be distinguished in having the very long rostrum which extends beyond the length of body. Most of species are monoecious holocyclic without host alternation and attended by ants. Stomaphis sinisalicis Zhang & Zhong, 1982 (Aphididae: Lachninae) is recognized for the first time in Korea, on Salix babylonica L., S. koreensis Anderss. and S. hulteni Floderus (Salicaceae).

The genus of Stomaphis (Aphididae: Lachninae) is stem or root feeding aphid on trees and can be easily distinguished in having the very long rostrum which extends beyond the length of body. Most of species are monoecious holocyclic without host alternation and attended by ants. Based on body appearance and COI gene sequence, we report a Stomaphis betulidahuricae Zhang and Qio, 1999 (Aphididae: Lachninae) for the first time in Korea, on Carpinus cordata Bl., C. laxiflora Bl., and Acer palmatum Thunb.

On Birches (Betula spp.), more than seventy species of aphids in eighteen genera are recorded worldwide, mostly (> 60%) belonging to ten genera of the subfamily Calaphidinae (Hemiptera: Aphididae). In Korea, only seven Birch feeding aphids are recorded. Recently, we recognized two calaphinid species, Calaphis betulaecolens (Fitch, 1851) and Euceraphis betulae (Koch, 1855) as new to the Korean peninsula.

The aphid genus Micromyzodium David, 1958 is newly recognized in the Korean peninsula. This genus is characterized by very long dorsal abdominal hairs, almost 3-4 times as long as basal diameter of antennal segment III. Colonies of two Micromyzodium species; Micromyzodium kuwakuse Uye 1924 and Micromyzodium nipponicum Moritsu 1949 were collected on Fatoua villosa Nakai (Urticales: Moraceae) and Perilla frutescens Britton (Lamiales: Lamiaceae), respectively. In this study, we redescribed, illustrated, and measured for apterous viviparous females of the two species.

The exotic aphid pest, pomegranate aphid, Aphis punicae Parsserini, 1863 (Hemiptera: Aphididae), is newly recognized in the Korean peninsula. This species is originally distributed throughout the Mediterranean regions, the Middle East, Ethiopia, India, Pakistan, Indonesia, and Turkey. In the major producers of pomegranate such as India and the Mediterranean regions, this species is regarded as one of the most serious insect pests inflicting injury on pomegranate, Punica granatum Linnaeus. By sucking plant sap and excreting honeydew, aphids cause various plant diseases and virus infections which are more likely to occur as population size increases. Aphis punicae infests together commonly with Aphis gosspii Glover, and rarely with Aphis craccivora Koch and Aphis spiraecola Patch on P. granatum in Korea. In this study, we first compared two closely related species, A. gossypii and A. punicae, based on morphometric analysis to confirm their morphological relationships. Second molecular identification using mitochondrial COI barcode region was performed for the aphid samples collected from P. granatum.

The cotton aphid, Aphis gossypii Glover (Hemiptera: Aphididae), is one of the major insect pests of cucumber, Cucumis sativus L. The spatial distribution and occurrence of Aphis gossypii Glover on greenhouse cucumber, Cucumis sativus L., were studied to provide an efficient and reliable sampling plan for its control. A highly clumped pattern was observed among plants (TPL b = 1.63) within a greenhouse. To develop a sampling plan for visual enumeration, leaves within plants were selected by different percentages of leaves (eg., 3rd and 6th, 6th and 9th leaf,…from the bottom of the plant) and then compared the accuracies and efficiencies by relative net precision (RNP). The results indicated that selecting leaves from the bottom of the plant by systemic sampling methods were relatively high as other sampling methods.