Diesel vehicle is regulated EURO 5 and will be apply to EURO 6 in 2014. Thus, researches have been performed to meet the strict emission regulations. We have obtained that the results of engine performance operated by lard biodiesel at various engine load and speed was slightly decreased, but brake specific energy consumption (BSEC) at using animal biodiesel below 15% was better than diesel fuels. When the mixing ratio of biodiesel increased, NOx was a slight increase. But PM and CO was a significantly reduced. Therefore, that the biodiesel of animal fats is available for eco-friendly fuel of diesel engine was confirmed.