A fumigant, methyl bromide (MB), has been widely used to control insect pests on various post-harvest agricultural products. Due to its negative side effects, such as ozone deplete and phytotoxicity, MB should be replaced and alternative control technique ought to be developed. Furthermore, organic agricultural products need to be treated with non-chemical methods. To meet these needs, controlled atmosphere and temperature treatment technique (CATTS) has been developed to control insect and mite pests infesting various post-harvest fruits. CATTS uses heat treatment at 46℃with a gradual increase of temperature program under ananoxia condition (less than 1% O2 and 15% CO2). The heating rate at ramping step is crucial to prevent any heat damage to fruits by mimicking natural conditions during summer period experienced by fruits in orchards. The exposure period to the high temperature is minimized by anoxia condition, which prevents any recovery metabolism occurring in the target insect/mite pests after the heat treatment. To develop MB alternative technique for exporting apples, CATTS conditions have been determined against several quarantine insect/mite pest, which are restricted from Korean apple import countries. Target insect pests include Grapholita molesta, Adoxophyes paraorana, Tetranychus viennensis, Conogethes punctiferalis, and Carposina sasakii. In each insect species, differences in susceptibility to high temperature were analyzed in all developmental stages. The highest tolerant developmental stages were adults in T. viennensis, but the fifth instar larvae in G. molesta, C. punctiferalis, C. sasakii, and A. paraorana. Gas conditions of CATTS kept 1% O2 and 15% CO2. After the internal fruit temperature increase from 25℃ to 44℃ during ramping stage (120-150 min), CATTS treatment lasted for 1-2 h. Under 1 h CATTS treatment, 100% mortality was recorded in all assays using more than 3,000 individuals of each species of T. viennensis adults, G. molesta fifth instar, C. sasakii fifth instar, and A. paraorana fifth instar. With 2 h CATTS treatment, 100% mortality was recorded in C. punctiferalis assay using 3,022 fifth instar larvae. Under 1 h CATTS treatment, no fruit damage was recorded in Fuji variety of apples. Treatment cost of CATTS is higher than that of methyl bromide (MB) treatment. However, once CATTS is equipped with nitrogen generator, it would be cheaper than MB in treatment cost. CATTS is a promising post-harvest pest control technique to replace MB treatment. Especially, CATTS would be an ideal control technique to disinfect any pests against organic agricultural products.

수확 후 해충방제가 국내 수요 농산물은 물론이고 검역 문제를 해결하기 위한 수출용 농산물에 대해서 요구되고 있다. 특별히 유기 농산물 또는 환경친화형 재배 농산물에 대해서 메틸브로마이드와 같은 화합물을 이용한 기존의 수확 후 처리기술은 의미를 잃게 되었다. 대체 기술로서 CATTS (환경조절열처리기술)라 명명된 물리적 처리기술이 개발되어 사과와 여러 핵과류 과실을 가해하는 곤충과 응애에 적용되고 있다. 본 연구는 국내 사과 수출을 위해 수입국에서 규제하는 복숭아순나방(Grapholita molesta)을 대상으로 CATTS 처리 조건을 결정하는 데 목표를 두었다. 이 해충에 CATTS를 적용하기 위해 사과 과실에 잔류하면서 열에 높은 내성을 보이는 발육시기를 분석하였다. 열처리 조건(44℃, 20분)에서 5령 유충이 가장 높은 내성을 보였다. 환경조건(15% CO2, 1% O2)에서 기기 내부 온도가 25℃에서 46℃까지 증가하는 시기를 CATTS 가열단계로 볼 때, 이 가열 속도가 빠를수록 CATTS 해충 방제 효과가 높았다. 또한 가열단계 후 CATTS 처리 시간이 길수록 CATTS 효율이 증가했다. 특히 가열단계에서 과실 내부온도가 44℃로 이르게 하는 것이 CATTS 효율을 높이는 데 결정적이었다. 이러한 조건들을 종합하여 CATTS표준 처리기술을 결정하였으며, 이 기술은 2,104 마리의 5령을 포함한 4,378 마리 복숭아순나방 유충 피해를 받은 사과에 대해서 100% 방제 효과를 나타냈다.

환경조절열처리는 일명 CATTS (controlled atmosphere temperature treatment system)로 특정 해충의 생존 한계에 해당하는 고온을 처리하면서 방역효과를 극대 화하는 기술로써 수확 후 소독 처리 기술인 메틸브로마이드 훈증처리를 대체할 친 환경 수확 후 소독 기술로 개발되고 있다. 복숭아심식나방(Carposina sasakii)은 수출국의 검역 대상 해충이다. 본 연구는 국내 사과를 일부 외국으로 수출할 경우 검역대상으로 지목받고 있는 복숭아심식나방에 CATTS 기술을 적용하였고, 이에 따른 수출용 후지 사과의 품질을 조사하였다. 소독 처리에 사용된 CATTS는 시간 당 16℃씩 증가하여 기기의 내부 온도가 46℃가 되었을 때 이산화탄소 15%, 산소 1% 환경 조건을 맞추어 주었고, 과실 내부의 온도가 44℃에 도달하였을 때 소독 처리를 시작하였다. CATTS 소독 처리는 0 분, 30 분, 60 분, 75 분, 90 분, 120 분, 150 분과 180 분으로 나누어 처리하였다. CATTS 소독 처리 후 후지 사과는 상온 (25℃) 조건에서 1 일, 7 일과 14 일 저장하였고, 저온(1℃) 조건에서 1 일, 7 일, 14 일, 30 일, 60 일, 75 일, 90 일과 120 일 저장하였다. 저장 시기별로 사과 품질을 조사하였다. 사과 품질은 이화학 품질 특성(경도, 당도와 산도), 호흡 속도(에틸렌, 이산화탄소)와 외부･내부 갈변율을 조사하였다. 이화학 품질 특성과 호흡 속도에 따른 차이가 없었으며, 60 분 처리 시간을 기준으로 처리 시간과 저장 기간이 증가 에 비례하였다.

A postharvest treatment called CATTS (controlled atmosphere and temperature treatment system) has been used as an alternative nonchemical measure for methyl bromide fumigant treatment. This study applied CATTS to control the red flour beetle, Tribolium castaneum, infesting stored grains. Adults of T. castaneum were susceptible to 46℃ heat treatment. The susceptibility was further enhanced by addition of CA conditions (15% CO₂ and 1% O₂). When CATTS (46℃, 15% CO₂, 16℃/h treating rate) was applied to different developmental stages of T. castaneum, it showed 100% control efficacy by 120 min exposure. There was a variation in CATTS susceptibility among developmental stages, in which late instar larvae were most tolerant. Heat shock proteins of T. castaneum appeared to be implicated in the tolerance of CATTS.

As an environment-friendly phytosanitary measure, CATTS (controlled atmosphere temperature treatment system) has been developed to kill several quarantine insect pests infesting subtropical agricultural commodities. This study tested any possibility to apply CATTS to apples to effectively eliminate the peach fruit moth, Carposina sasakii, which has been regarded as a quarantine insect from the imported countries. When the larvae of C. sasakii were directly exposed to 46℃ (an installed lethal temperature of CATTS), they showed a median lethal time at 14.66 min. Addition of high carbon dioxide to the temperature treatment enhanced the thermal limit susceptibility of C. sasakii to 46℃. The larvae internally infesting apples were tested using this CATTS device and showed 100% lethality after 60 min exposure to a treatment of 46℃ under 15% CO2 in the chamber. This study suggests a possibility that CATTS can be applied as a quarantine measure to kill the larvae of C. sasakii locating inside the apples. To understand the CATTS effect, a heat shock protein was cloned. Hsp90 was partially sequenced and showed its expression in response to heat treatment. CATTS was likely to suppress hsp90 expression.

As an environment-friendly phytosanitary measure, CATTS (controlled atmosphere temperature treatment system) has been developed to kill several quarantine insect pests infesting subtropical agricultural commodities. This study tested any possibility to apply CATTS to apples to effectively eliminate the peach fruit moth, Carposina sasakii, which has been regarded as a quarantine insect from the imported countries. When the larvae of C. sasakii were directly exposed to 46℃ (an installed lethal temperature of CATTS), they showed a median lethal time at 14.66 min. Addition of high carbon dioxide to the temperature treatment enhanced the thermal limit susceptibility of C. sasakii to 46℃. CATTS device was constructed to automatically control CO₂ concentration and temperature with real-time monitoring both in the chamber and in the fruit. The larvae internally infesting apples were tested using the CATTS device and showed 100% lethality after 60 min exposure to a treatment of 46℃ under 15% CO₂ in the chamber. Relatively long exposure may be due to the deviation between the ramping temperature (0.35℃/min) of the chamber and the ramping temperature (0.12-0.23℃/min) inside apple fruit, where the tested larvae were located. This study suggests a possibility that CATTS can be applied as a quarantine measure to kill the larvae of C. sasakii locating inside the apples.