For countering nuclear proliferation, satellite imagery is being used to monitor suspicious nuclear activities in inaccessible countries or regions. Monitoring such activities involves detecting changes over time in nuclear facilities and their surroundings, and interpreting them based on prior knowledge in terms of nuclear proliferation or weaponisation. Therefore, analysts need to acquire and analyze satellite images periodically and have an understanding of nuclear fuel cycle as well as expertise in remote sensing. Meanwhile, as accessibility of satellite information has been increasing and accordingly a large amount of high-resolution satellite images is available, a lack of experts with expertise in both fields to perform satellite imagery analysis is being concerned. In this regard, the Institute of Korea Nonproliferation and Control (KINAC) has developed a prototype of semi-automatic satellite imagery analysis system that can support monitoring of potential nuclear activities to overcome the limitations of professionals and increase analysis efficiency. The system provides a satellite imagery database that can manage acquired images, and the users can load images from the database and analyze them in stages. The system includes a preprocessing module capable of resizing, correcting and matching images, a change detection module equipped with a pixel-object-based change detection algorithm for multi-temporal images, and a module that automatically generates reports with relevant information. In particular, this system continuously updates open-source information database related to potential nuclear activities and provides users with an integrated analytics platform that can support their interpretation by linking related images and textual information together. As such, the system could save time and cost in processing and interpreting satellite images by providing semi-automated analytic workflows for monitoring potential nuclear activities.

범밀도이론함수(Density Functional Theory, DFT) 기반의 제일원리전산모사는 기저상태의 DFT 에너지를 구하는데 많은 시간소요 및 전산자원을 소모하였다. 이러한 막대한 전산자원의 소모는 DFT 계산에서 고려할 원자수를 수 백개 이 하로 제한되게 되었으며, 이를 해결하기 위해서는 전자구조 계산이 아닌 원자의 환경 내에 원자간 상호작용을 정의 (Force Field, 힘장)하고 이를 통해 주어진 조성 혹은 구조에 따른 에너지를 빠르게 예측 할 수 있어야 한다. 본 논문에서 는 Behler-Parrinello가 제시한 인공신경망 모델을 활용해 인공지능 다원계 힘장을 개발하고 코발트-구리 산화물의 조성에 따른 에너지를 예측하고 안정한 구조를 탐색하는 연구를 수행하였다. 인공신경망 기술로 부터 구리-코발트 산화물에 대 해 15.7 meV/atom의 에너지 오차와 단위거리당 힘 103.6 meV/Å의 정확도를 가지는 인공신경망 포텐셜을 개발하였다. 이 방법으로 빠르고 정확하게 CuCoO 표면구조의 산소 결함률에 따른 생성에너지를 계산할 수 있었고, 에너지 컨벡스 홀을 도시 조성에 따른 안정한 구조를 예측하였다.

Direct injection of genome editing tools such as CRISPR/Cas9 system into developing embryos has been widely used to generate genetically engineered pigs. The approach allows us to produce pigs carrying targeted modifications at high efficiency without having to apply somatic cell nuclear transfer. However, the targeted modifications during embryogenesis often result in mosaicism, which causes issues in phenotyping founder animals and establishing a group of pigs carrying intended modifications. This study was aimed to establish a genomic PCR and sequencing system of a single blastomere in the four-cell embryos to detect potential mosaicism. We performed genomic PCR in four individual blastomeres from four-cell embryos. We successfully amplified target genomic region from single blastomeres of 4-cell stage embryo by PCR. Sanger sequencing of the PCR amplicons obtained from the blastomeres suggested that PCR-based genotyping of single blastomere was a feasible method to determine mutation type generated by genome editing technology such as CRISPR/Cas9 in early stage embryos. In conclusion, we successfully genotyped single blastomeres in a single 4-cell stage embryo to detect potential mosaicism in porcine embryos. Our approach offers a simple platform that can be used to screen the prevalence of mosaicism from designed CRISPR/Cas9 systems.

Logistics companies are worrying about securing of differential competitiveness so as to be competitive companies in keen logistics market. The ground is how users are satisfied by sell-established service system to respond not only economic feasibility of logistics costs but also diversity and advancement of logistics needs. The competitiveness of logistics companies is also caused by customer satisfaction of service and only companies finding and satisfying customer needs continuously may be more competitive. For the competitiveness, it’s the most important to analyze demands of current and potential customers and their pursuing value properly. Therefore, this researcher grasped PSL for online logistics service users with 5-point Likert-scale and quality-level decision method that consider the weighted value based on Kano model, measured customer’s potential Demand for service through PCDI, and suggested methodology for deciding the priority of the improvement with loss function of Taguchi.

Recently, service quality must reflect several demands of customers who show rapid and various changes so as to be compared with the past. So, objective and rapid measuring methods for service quality are necessary. For them, first of all, service company must calculate their standard of service quality accurately by measuring service quality exactly. Kano classified the degree of influence that is the degree of correspondence of the quality attributes of products and services to the subjective satisfaction of customers. As a result, the types of qualities are classified as attractive, must be, one dimensional, and indifference attributes. They have been widely used quality attributes in various industrial fields up to now. However, Kano model has a limit that it ignores the characters of the next frequent numbers even though there are not much gap comparing to the most frequent number in the questionnaire answers. The limit is attributed to the character of Kano model that the most frequent number is accepted as the only quality character. Timko calculated the customer satisfaction coefficient by using Kano’s method and studied the differences in quality character by classifying the quality characteristics in a graphical way through the relationship between the satisfaction and the dissatisfaction coefficient. In this study, we used the quality level determination method of the 7-point Likert scale, which takes the weight into account, to complement the deficiencies of the existing Kano model. We also developed and applied a Potential Satisfaction Level (P) and Potential Customer Demand Improvement (PCDI) Index to present a new approach to the determination of service quality attributes. To measure the level of potential service satisfaction and to understand the degree of improvement, we collected specimens of 51 participants who has been trained in the National Strategy Business Training Program, which has been managed by government agent, and analyzed the results.1)

본 연구에서는 한반도 주변 화산분화 시나리오를 고려한 국내 농업시설물의 화산재 취약도를 구축하였다. 이를 구축하기 위하여 화산재 하중 통계치(평균, 표준편차)와 농업시설물 저항성능의 통계치를 통해 신뢰도 지수를 산정하는 FOSM(first-order secondmoment) 기법이 이용되었다. 화산재 하중 통계치는 화산재 확산 시뮬레이션인 FALL3D를 이용하여 백두산, 울릉도, 아소산에 대한 분화 시나리오를 고려하여 산정하였으며, 농업시설물의 저항성능 통계치는 농촌진흥청에서 제공하는 내재해형 비닐하우스의 설계 적설심을 통해 공칭 파괴강도에 기초하여 산정하였다. FOSM 기법으로 평가된 화산재 취약도는 연속적인 화산재 퇴적두께로 인한 파괴확률을 평가하기 위하여 GEV 누적분포함수의 모수 형태로 데이터베이스화하였다. 본 연구에서 구축된 농업시설물의 화산재 취약도는 분화 화산별(백두산, 울릉도, 아소산), 지역별(서울, 부산, 대구, 대전), 농업시설물별(07-자동화-01, 08-자동화-01, 10-자동화-01), 화산재 수분함유 상태별(습윤, 건조)로 각기 상이한 파괴확률을 평가하였으며, 추가적으로 구축된 농업시설물의 화산재 취약도를 비교·분석하여 최대/최소의 파괴확률 범위를 확인하였다.

Recently, service quality must reflect several demands of customers who show rapid and various changes so as to be compared with the past. So, objective and rapid methods for them are necessary more. For them, first of all, service company must calculate their standard of service quality accurately by measuring service quality exactly. To measure service quality accurately, this researcher collected and analyzed data by survey for customers who are customers of logistics services, grasped potential satisfaction standard(P) by 5 point Likert scale and one survey for accurate classification of quality attributes through weighted customer satisfaction coefficient changing quality attributes by developing the study on Kano model and Timko's customer satisfaction coefficient, and suggested Potential Customer Satisfaction Improvement index(PCSI) for examining the improvement of customer satisfaction so as to utilize them as an index of differentiated and concrete measurement of service quality.

The fungal mycelium-based composite is a new biomaterial to replace the existing composite material. To compete with lightweight, high-performance composites represented by fiber-reinforced plastic, various physical and chemical properties and functionality must be secured. Especially, the composite material made by using mushroom mycelium (or fruting bodies) is called mushroom plastic. Currently, Ecovative, Mycoworks, and Muskin in USA and Europe are launching new products including structural materials and leathers. Products utilizing mushroom mycelium can be launched in the market for construction materials, automobile interior materials and artificial leather substitutes. In spite of this high possibility, mass production using FMBC has not yet been reported. This presentation introduces the mycelium-based materials, a material that can replace existing plastics, inorganic building materials and animal skins in an environmentally and economically viable way, and looks at the possibility of future biomaterials by summarizing recent research contents.

Microfluidics based on nanobio sensors technologies can provide convenient and accurate diagnosis tools. In this talk, we present recent developments of nanobio sensors & diagnosis chip using microfluidics, with special emphasis on disposable plastic devices format. In detail, we overview of the common methods used in the fabrication of polymer microfluidic systems, including replica and injection mold-ing. Also we explain the different methods by which on-chip operations—such as the pumping and valving of fluid flow, the mixing of different reagents, and the separation and detection of different biochemical species implemented in a microfluidic format. Finally, a few select biotechnological applications of microfluidics are presented to illustrate both the utility of this technology and its potential applications with insect models in the near future.

본 연구는 우리나라 항만 고유의 기능 및 특성과 지역의 정체성을 접목하여, 그 가치를 평가 할 수 있는 항만 경관 잠재력 평가지표 도출 및 중요도 분석을 하였다. 연구 목적 달성을 위해 첫째, 항만 경관 잠재력 평가지표 개발을 위한 선행 연구 및 문헌분석 후 하위 평가지표를 도출하였다. 둘째, 전문가 인터뷰를 통해 객관적으로 평가 할 수 있는 상위지 표 기준을 도출 하여 상위 평가지표를 선정하였다. 그리고 그 평가지표를 기준으로 계층 모형을 구축하여 항만관련 분야 의 공무원(해양경찰), 공기업(항만공사), 설계기관(엔지니어링), 학계(교수, 연구원)의 전문가 집단 70명을 대상으로 평가 지표에 대한 설문 조사를 실시하였다. 설문 분석 방법으로는 응답자 속성을 알기 위해 SPSS 20.0.ver을 활용하여 빈도분 석을 실시하였으며, 항만의 경관 잠재력 평가지표를 도출하여 중요도 및 우선순위를 알기 위해 AHP(Analytic Hierarchy Process)분석에서 보편적으로 쓰이는 Expert Choice 11.5.ver의 프로그램을 사용하였다. 분석결과 첫째, 항만 경관 잠재력 상위 평가지표 4개(항만의 기능, 항만의 경관자원, 파급효과, 지속가능) 와 하위지표 12개(항만의 규모, 항만의 접근성, 항만의 이용실적, 자연자원, 인문자원, 시점자원, 랜드마크자원, 항만의 시설자원, 배 후 세력권, 홍보 기회, 지자체 역량, 수요예측)를 도출하였다. 둘째, 상위지표는 항만의 기능과 항만의 경관자원이 상대적 으로 높게 나타났다. 셋째, 하위지표는 항만의 기능은 항만의 접근성이, 항만의 경관자원은 자연자원이, 파급효과는 배후 세력권이, 지속 가능은 지자체 역량이 상대적으로 높게 나타났다. 넷째, 종합 중요도 및 우선순위 분석 결과 항만의 접근 성, 자연자원, 배후 세력권, 랜드마크 자원이 상대적으로 높게 나타났다. 향후 본 연구의 결과를 토대로 항만 고유의 정체성을 반영한 항만 경관계획이 필요할 것으로 판단된다.

The spotted-wing drosophila (SWD), Drosophila suzukii (Diptera: Drosophilidae), originally distributed across a few Asian countries, including South Korea, has invaded North America and Europe, but is absent from Australia. In order to export the South Korean grape cultivar Campbell Early to Australia, its potential to serve as oviposition and development medium for SWD must first be determined. In this study, we determined the oviposition and development potential of SWD on Campbell Early, after elucidating the SWD life cycle and establishing an artificial diet-based mass-culturing system. An investigation of the life cycle under five temperature regimes (16, 19, 22, 25, and 28°C) showed that the durations of the egg, larval, and adult stages were shorten when temperature was increased from 16, 19, 22, 25, and 28°C, but pupal duration was shortest at 25°C and extended again at 28°C. A test of oviposition and development potential of SWD on Campbell Early grape clusters showed oviposition of 30.8 ± 6.8 eggs per cluster of injured grapes and 157.7 ± 16.2 eggs on a culture dish of artificial diet. However, in a similar experiment using uninjured grape clusters, only a single egg was deposited on the grape skin, which soon dried. In light of these results, newly harvested grapes left at vineyards during daily harvests are unlikely to serve as an oviposition and development medium for SWD, as long as the grapes remain uninjured.

To improve the developmental potential of bovine somatic cell nuclear transfer (SCNT) embryos, this study compared the developmental rates to blastocyst stage in the SCNT embryos using donor fibroblasts treated with 5-azacytidine (5AC) and S-adenosylhomocysteine (SAH) at different concentrations. Their reprogramming efficiency level was investigated with level of telomerase activity. Donor fibroblasts isolated from adult ear skin of a cow were exposed to 5AC and SAH at different concentrations during 2 passages. After nuclear transfer into enucleated recipient oocytes, the cleavage and developmental rates were significantly (p<0.05) decreased in the SCNT embryos using 5AC-treated fibroblasts (5AC-SCNT embryos), compared with those of non-treated control (control-SCNT embryos) and SAH-treated fibroblasts (SAH-SCNT embryos). The developmental rates to blastocyst stage tended to be slightly increased in the SAH-SCNT embryos at each of the concentrations, and especially, the developmental rates in the SCNT embryos using 1.0 mM SAH-treated fibroblasts were significantly (p<0.05) higher than that of control SCNT embryos. The mean numbers of total and ICM cell in blastocysts were also significantly (p<0.05) decreased in the 5AC-SCNT embryos, compared with those of other SCNT blastocysts. Further, the level of telomerase activity was also significantly (p< 0.05) decreased in the 5AC-SCNT embryos than those of control and SAH-SCNT embryos. Whereas, a significantly (p<0.05) up-regulated telomerase activity was observed in SAH-SCNT embryos, compare with that of control-SCNT embryos. In conclusion, SCNT embryos using hypomethylated donor cells with SAH, not 5AC, may improve the developmental potential and reprogramming efficiency.

PURPOSES : This study is to develop ISM for potential risk factor in School Zone. METHODS : Based on the literature review, the Analytic Hierarchy Process (AHP) has been used most widely. However, it is difficult to apply in practice because the AHP results have the characteristics of the independence between each element and the interlayer can not explain the interrelationship. The Network Analysis Process (ANP) is possible to analyze the relationship between the elements and the network through the feedback. But, the reliability of the analysis fall because of complicated pair of comparison, also it is difficult to solve the super matrix. In this study, the complicated relationship between each element is inquired through the Interpretive Structural Modeling (ISM). RESULTS : The methodology of ISM is developed to remove the children's potential risk factors in school zone. CONCLUSIONS : It is possible to remove the children's potential risk factors from low level to high level step by step and improve safety. Through this, risk factors can be removed from the low-level, and upper-level will automatically improve.

In all the studies of mammalian species, chromatin in the germinal vesicle (GV) is initially decondensed with the nucleolus not surrounded by heterochromatin (the NSN configurations). During oocyte growth, the GV chromatin condenses into perinucleolar rings (the SN configurations) or other corresponding configurations with or without the perinucleolar rings, depending on species. During oocyte maturation, the GV chromatin is synchronized in a less condensed state before germinal vesicle breakdown (GVBD) in species that has been minutely studied. As not all the species show the SN configuration and gene transcription always stops at the late stage of oocyte growth, it is suggested that a thorough condensation of GV chromatin is essential for transcriptional repression. Because the GV chromatin status is highly correlated with oocyte competence, oocytes must end the NSN configuration before they gain the full meiotic competence and they must take on the SN or corresponding configurations to stop gene transcription before they acquire the competence for early embryonic development. In this study, we firstly investigated whether the follicle size could determine chromatin configuration in porcine oocyte. For this experiment, follicles was divided into three groups (<1 mm follicle, 1～3 mm follicle and 3～6 follicle). Using DAPI staining, the GV nucleolus and chromatin of porcine oocytes was classified into SN, SN-NSN and NSN configurations. MⅠ and M Ⅱ of three groups's Mature oocytes by staining was confirmed the configuration of chromatin. The maturation rate and parthenogenetic development potential were significant different between the SN and NSN configurations oocytes. These results indicated that chromatin changes in GV oocytes affect the development potential of porcine embryos.

본 연구는 국공립 직업무용단체 11단체의 웹사이트를 마케팅믹스 관점에서 운영되어지고 있는 현황을 분석하여 웹사이트를 통한 관객개발 전략이 나아갈 방향성을 제시하는 데 목적이 있다. 본 연구 방법은 마케팅 믹스 개념에 대한 문헌조사를 실시하였으며, 연구대상은 일정한 급여를 정기적으로 부여하는 직업무용단 중 웹사이트를 운영하고 있는 국공립 단체를 선정하였다. 분석방법은 일반경영학에서 제시하는 마케팅 믹스(Production, Price, Promotion, Place) 4가지 요소를 응용하여 예술경영의 차원에서 마케팅믹스 요소들을 도출하여 각각의 영역이 웹사이트 구성내용에 어떻게 분포되었는지를 분석하였다. 분석된 자료는 선행연구 정미란(2007)의 “국내직업무용단의 웹사이트 콘텐츠 비교분석”과 신동윤 이성민(2004) “한국프로축구단 마케팅 믹스 요소 및 일반적 항목 웹사이트 내용분석”에서 제시한 분류방법을 수용하여 처리하였으며, 마케팅 믹스 4가지 요소가 나타난 빈도수와 내용을 분석하였으며, 그 결과를 도출하여 다음과 같은 전략을 제시하였다. 첫째, 국공립단체의 독립 웹사이트 운영을 통해 공연에 관심을 갖는 잠재관객의 관심도를 높일 수 있을 것이다. 둘째, 인터넷을 활용하여 무용단체의 웹사이트 방문을 유도하기 위해서 다양한 검색단어에 관련단어를 넣으면 최대한 많이 공연단 웹사이트에 링크 되도록 하여 사람들에게 친밀감을 주어 관심을 갖도록 유도할 필요가 있다. 셋째, 웹사이트 상에서 회원들이 참여할 수 있는 공간의 확대를 통해 보다 많은 홍보를 유도할 수 있는 것이다. 넷째, 웹사이트에서 이벤트와 공연의 연계성을 확대하여 공연장에 관객이 직접 참여할 수 있도록 유도하면서 티켓 구매 효과를 증대할 필요가 있다. 다섯째, 무용단체의 웹사이트 운영에 있어서 관객과 단체 간의 실시간 커뮤니티장의 마련을 통해서 단체와 관객의 직접적인 만남을 유도할 필요가 있다.

본 연구에서 돼지 난포란에서 채취된 난모 세포들을 체외성숙 후 형태적으로 선별하거나 극체 방출란을 선별하여 활성화 처리 후 48시간째에 분할란을 선별할 때 배발달율이 어느정도 향상되는지를 검토하였다. 난모 세포를 48시간 성숙 배양 후 형태적 선별과 극체의 방출 유무를 검사하고, 선별된 난모 세포들을 시간 추가 배양한 후 7% ethanol로 활성화시키고 cytochalasin B에 5시간 노출 후 PZM-5 배 양액으로 7일간 배양하였으며, 배양 중