A new WRKY transcription factor gene was isolated by ESTs screening from a cDNA library of suspension cultured cells of Sweet potato (Ipomoea batatas). The 2,285 bp cDNA fragment, IbWRKY, was sequenced, from which a 505 amino acid residue protein was deduced. A search of the protein BLAST database identified significant similarity to other plant WRKY31 protein sequences. RT-PCR analysis showed expression patterns of IbWRKY31 in various intact tissues and suspension cultured cells of Sweet potato, and in leaves exposed to different stresses. The IbWRKY31 gene was highly expressed in suspension cultured cells. In leaf tissues, IbWRKY31 showed strong expression during salicylic acid and methyl jasmonate treatments. Expression of IbWRKY31 was also induced under various abiotic stress and pathogen infection conditions, such as wounding, H2O2, MV, PEG, NaCl, and bacterial pathogen infection. These results suggest that IbWRKY31 is involved in plant responses to various stress conditions, such as abiotic stresses and pathogen infection through a defense signaling pathway.

본 연구는 치매환자를 돌보는 간호사의 공감도 구성요인이 무엇인지 분석하여, 이를 통해 치매 환자 간호실무에 도움이 되고자 한다. 본 연구를 수행하기 위해 요양병원에서 치매환자 간호에 종사하는 219명의 간호사를 대상으로 하였다. 공감도(ECRS)를 이용하여 요인구조 탐색과 확인요인분석(CFA)을 통 한 기초모형 추출 및 모형 수정, 모형 적합성 검증을 실시하였다. 본 연구 결과 간호사의 치매환자에 대한 공감도는 타인 정서의 수용, 자신의 부정정서 억제, 정서의 교류 및 상호작용 등 3개요인 49개 문항으로 구성요인의 타당성과 신뢰성을 확인하였다. 이러한 결과에 따라 타당성과 신뢰성을 확보한 공감도 측정 도 구는 향후 치매환자 간호실무현장에서 간호사의 공감도를 확인하는 연구에 활용될 수 있을 것으로 기대된 다.

The fruit of Chaenomeles sinensis (Thouin) Koehne (Chaenomelis Fructus) known as “Mo-Gua” in Korea has been commonly used in traditional medicine to treat inflammatory diseases, such as sore throat. However, its effect on bone metabolism has not been elucidated yet. Here, we examined the effect of Chaenomelis Fructus ethanol extract (CFE) on receptor activator of nuclear factor (NF)-κB ligand (RANKL)-mediated osteoclast differentiation and formation. CF-E considerably inhibited osteoclast differentiation and tartrate-resistant acid phosphatase-positive multinuclear cell formation from bone marrow-derived macrophages and osteoclast precursor cells in a dose-dependent manner. In addition, the formation of actin rings and resorption pits were significantly suppressed in CF-E-treated osteoclasts as compared with the findings in non-treated control cells. Consistent with these phenotypic inhibitory results, the expressions of osteoclast differentiation marker genes (Acp5, Atp6v0d2 , Oscar, CtsK, and Tm7sf4) and Nfatc1 , a pivotal transcription factor for osteoclastogenesis, were markedly decreased by CF-E treatment. The inhibitory effect of CF-E on RANKL-induced osteoclastogenesis was associated with the suppression of NFATc1 expression, not by regulation of mitogen-activated protein kinases and NF-κB activation but by the inactivation of phospholipase C gamma 1 and 2. These results indicate that CF-E has an inhibitory effect on osteoclast differentiation and formation, and they suggest the possibility of CF-E as a traditional therapeutic agent against bone-resorptive diseases, such as osteoporosis, rheumatoid arthritis, and periodontitis.

This study aims to show that language purification policies are the most important factors that influence Korean language differentiation, based on the premise that Korean is a pluricentric language having two regional varieties: South Korean P’yojuno and North Korean Munhwao. To this end, on the basis of a precedent study that analyzed regional variation mechanisms in French through four factors—terminological, socio-terminological, psycho-terminological, and extra-terminological—we identified two factors having great influence on the language change in South and North Korean societies: socio-terminological and extra-terminological. We also found that these factors precisely correspond to two categories in Kloss's(1969) theory of language planning— corpus planning and status planning; this confirmed that we should consider Korean language differentiation from the standpoint of language policy. There are many factors that cause language differentiation, and there is no doubt that these factors also have complex interrelationships. However, this study is all the more meaningful in that it uses systematic analysis to state that language policy plays an important role in Korean language differentiation.

비정형 형태의 건축물에서 발생하는 풍하중은 KBC-2016의 풍하중 산정식으로 산정할 수 없기 때문에 풍동실험을 통해 풍하 중을 평가할 수밖에 없다. KBC-2016으로 비정형건축물을 정형적인 건축물로 가정하여 풍하중을 평가한다면 과소평가될 우려가 있 다. 그러므로 보다 합리적인 평가를 위해 풍하중을 할증시켜줄 필요가 있다. 본 연구에서는 평면형태가 L자인 건축물을 대상으로 풍력 실험을 실시하여 풍하중을 산정하였으며, 이를 KBC-2016으로 산정한 풍하중과 비교하였다. 풍동실험을 통해 구한 L자형 건축물의 풍 하중과 KBC-2016으로 L자형 건축물과 동일한 폭과 깊이를 가진 사각형평면 건축물을 대상으로 구한 풍하중의 비로 풍하중 할증계수 를 도출하였다. 풍하중 할증계수는 1.6~2.2로 나타났다. KBC-2016에 의해 평가한 사각형 건축물의 풍하중에 풍하중 할증계수를 곱하 면 L자형 건축물의 풍하중이 된다.

무도상 교량은 레일을 이음매판으로 부설하여 사용하고 있어 과다한 충격이나 궤도 틀림 등의 발생 우려로 인하여 열차의 고속주행이 어려운 실정이다. 열차의 주행안정성을 확보하기 위해서는 상호작용에 영향을 미치는 인자를 분석하여 동적 안전성에 대하여 검토할 필요가 있다. 이 연구에는 열차가 운용중인 판형교량의 현장계측을 통하여 궤도 구조 및 주거더의 충격계수를 도출하고 이를 기존연구와 비교 및 참고하여 열차의 주행속도를 고려한 충격계수의 추정식을 산정하였다. 궤도의 충격계수 산정식은 기존 연구에서 제안한 식과 유사하게 산정되었으며, 무도상교량 거더의 충격계수 산정식과 일본의 철도교 충격계수 식과 비교한 결과, 일본의 철도교 충격계수 식은 본 연구에서 산정한 거더의 충격계수 1배수 식과 2배수 식 사이에 존재하는 것으로 나타났다.

Interferon tau (IFNT) regulation, an anti-luteolytic factor produced by conceptuses of the ruminant ungulates, is essential for the maintenance of early pregnancy, but a definitive mechanism for its temporal transcription has not been elucidated. We and others have observed the T-box protein eomesodermin (EOMES) exhibited high mRNA expression in the ovine embryonic trophectoderm; thus, both caudal-relatedhomeobox-2 (CDX2) and EOMES coexist during the early stages of conceptus development. Objective of this study was to examine the effect of EOMES on ovine IFNT gene transcription when evaluated with CDX2, ETS2 and AP1 transcription factors implicated in the control of cell differentiation in the trophectoderm. In this study, quantitatively via reverse transcription-polymerase chain reaction (RT-PCR) analysis between ovine trophoblast cells was initially performed, finding that transcription factors CDX2 and ‘EOMES transcription factor mRNAs’ were specific to trophectoderm cells. These mRNAs were also found in days 15, 17, and 21 ovine conceptuses. Furthermore, human choriocarcinoma JEG3 cells (trophoblast cell line) were cotransfected with an ovine IFNT (-654bp)-luciferase reporter (-654-oIFNT-Luc) construct and several transcription factor expression plasmids. Cotransfection of the reporter construct with CDX2, ETS2 and AP1 increased transcription of -654-oIFNT-Luc by about 11-fold compared with transfection of the construct alone. When cells were initially transfected with EOMES followed by transfection with CDX2, ETS2 and/or AP1, the expression of -654-oIFNT-Luc was decreased. Also, EOMES factor inhibited the stimulatory activity of CDX2 alone. These results suggest that when conceptuses attach to the uterine epithelium, ovine IFNT gene transcription is down-regulated by an increase of EOMES factor expression in the attached ovine trophoblast cells.

Growth factors and cytokines play an important role in delaying skin aging. However, there has been no animal stability test reported for them yet. In the present study, we examined the plausibility of Growth factor and Cytokine Mixtures (GCM) as a cosmetic ingredient by assessing skin irritation and ocular irritation for hypersensitivity using New Zealand white rabbits. Skin safety study was performed to evaluate the potential toxicity of GCM based on the irritation test. For the irritation test, GCM was applied to the rabbit skin, and no adverse reaction, such as erythema and edema, had been observed from the exposed skin sites. In the ocular irritation test, the treatment of GCM did not cause any adverse reaction on rabbit eyes, including cornea, iris, and conjunctiva tissues. From the results, both tests proved that GCM makes no irritable reaction on skin and eye mucous membrane of rabbit. Hence, it may be suggested that GCM can be safely applied as a cosmeceutical ingredient without causing any significant hypersensitivity reaction.

This study attempts to improve the understanding of the rapidly growing online video platform market such as Youtube and OTT, and to investigate the attributes and relative importance of them. For this purpose, the factors that influence the choice to use were derived through literature studies and the Focus Group Interview (FGI), and the priority of the factors was calculated through the analytic hierarchy process (AHP). The upper layer of the AHP structure was ‘Relationship’, ‘Entertainment’, ‘Informativity’, and ‘Convenience’, and the lower layer was structured into 13 elements. The importance priority analysis among the factors that influence the choice to use was done by teenagers, 20s, and 30s and the results are summarized as follows : First, Users consider the ‘Just for fun’ and ‘Satisfaction of interests’ as the most important factors, followed by ‘Easy accessibility to use’, ‘Vicarious satisfaction’, ‘Usefulness of Information’, and ‘Up-to-dateness of information’. Second, the ranking of the upper layer was in the order of ‘Entertainment’-‘Informativity’-‘Convenience’-‘Relationship’.As a result of AHP,‘Entertainment’ was 3.6 times more important than ‘Relationship’. In the comparison by age group, only teenagers regarded that ‘Convenience’ is more important than ‘Informativity’. According to the characteristics of the age group, the lower layer of teenagers consider ‘Convenient function’ to be important and ranked ‘Usefulness of information’ in 8th. While ‘Vicarious satisfaction’ ranked 4th out of 13 factors in the entire age group, those in their 20s and 30s ranked 8th, showing a difference. In the case of 20s, ‘Reasonable price’ was ranked 4th and the ‘Diversity of Information’ was ranked 5th, Otherwise 30s consider ‘Trustworthiness of Information’ to the third. Third, unlike ‘Convenience’ which was the lower-rank in the upper layer AHP analysis, ‘Easy accessibility to use’, the lower-layer of convenience, ranked third overall in the importance analysis among the 13 lower-layer factors, and showed a similar patterns in the age groups results. In the conclusion, this study demonstrates that ‘Convenience’ and ‘Vicarious satisfaction’ factors, which were not relatively well addressed in the previous studies, are the key factors to be considered in. By presenting the results of the importance analysis on each of the selected attributes, This study has a practical implication that Industries such as on-line video service platform provider can use the importance priority in establishing the directions of future strategy.

A warehouse is more than just a place to store goods but also creates a time utility gap between production and consumption that is important in the development of trade and commerce. Malaysia has a high aspiration to be the most popular gateway in Asia. However, Malaysia Productivity Corporation has claimed that particularly in efficiency and productivity aspects, the warehouse industry is less incline to innovate and make improvement to increase. There are ten failure factors that had been identified during literature survey affecting to the warehouse operations efficiency. Therefore, this study intends to analyse and select the main failure factors that mostly affecting the warehouse operations efficiency. A Fuzzy Analytic Hierarchy Process (FAHP) technique has been used in this study to calculate the weight value of each variable through a pair-wise comparison method. The finding highlighted the four main failure factors which are ‘equipment’, ‘environment concern’, ‘special handling consideration’ and ‘human factor’ that influence directly to the warehouse operations efficiency. This paper has contributed to a new knowledge of complex warehouse operation area with recommendations action that shall be taken to manage the possible risk in future, which will also contribute to achieve the government goals.

모바일 MMORPG는 게임 시장을 주도하고 있고 더욱 발전할 것이다. 많은 게임의 출시에도 불구하고 UI등 디자인설계 요인에서부터 소프트웨어 품질요인까지의 종합적인 MMORPG 설계연구가 부족한 상황에서 사용자들이 원하고 선호하는 세부 설계요인을 찾는 것은 매우 중요하다. 본 연구는 모바일 MMORPG의 사용자와 개발자가 객관적인 시각에서 양자가 만족하는 핵심적이고 중요한 게임 설계 요인을 찾고자 시도되었다. 실증분석은 이론적인 연구들을 기반으로 모바일 MMORPG 설계 요인들 3단계로 계층화하여 그 중요도는 계층분석과정(AHP)을 통해 검증하였다. 설계 요인의 중요도는 재미 요인, 구성 요인, UI 요인, 시스템 요인, 환경 요인 순이고 재미의 중요성에서는 도전 재미가 가장 중요하며 도전 재미에서는 보상이 중요하다는 결과를 얻었다. 재미 요인 중에서 새롭게 추가한 우월성이나 대결성을 포함하는 경쟁 재미가 다양성, 탐험성을 포함한 상상 재미보다 높게 나온 다. 이는 수많은 사용자가 길드, 혈맹, 진영 종족단위로 경쟁하고 협동하며 즐기는 모바일 MMORPG의 특성을 반영한 것으로 생각된다. 본 연구는 MMORPG 설계 요인들을 분석 추출 하였으며 중요도를 파악하여 모바일 MMORPG 설계 능력 향상에 기여할 것으로 생각한다.

When the foundation work of the underground part of the building structure or the excavation work of the civil engineering structure is carried out, there is the earthwork work by the inevitable process. As the economic situation continues to develop, construction in urban areas is becoming bigger and higher in scale due to the expansion of infrastructure and the rescue of urban dwellings in urban areas, and excavation of underground roads is inevitable. Excavation of the underground part may cause problems in the process difficulty and safety of the earthworks due to the complexity and various characteristics of the ground selected without consideration of the ground characteristics and site conditions. In order to complete the required facilities, it is necessary to secure the design and construction of the retaining walls. In order to complete the required construction, It is an important factor satisfying construction period and economical efficiency.

Proteasome inhibitors can improve the efficiency of cancer treatments by inhibiting nuclear factor κB(NF-κB) activation in cancer cells. Lentils are a type of beans of which consumption of such beans is increasing. The purpose of this study was to investigate the effects of lentils extract (LE) on the proteasomal activities, NF-κB activation, and cell cycle in HepG2 human liver cancer cells. LE treatments inhibited proteasomal activities at concentrations of 10, 50, and 100 μg/mL respectively, and repressed NF-κB activation at concentrations of 1, 10, and 100 μg/mL respectively, in HepG2 cells. LE treatments at concentrations of 1, 10, and 100 μg/mL respectively, increased sub-G1 cell population in HepG2 cells, which may be the result of apoptosis. The results suggest that LE inhibited NF-κB activation partially with its proteasome inhibitory activities, and the increase of sub-G1 cell population was induced partially, by inhibition of NF-κB activation in HepG2 cells.

본 연구는 퇴적물이 소모하는 산소량(SOD)과 환경 인자가 서로 미치는 영향을 파악하기 위해 퇴적물 배양실험을 수행하였다. 이를 위해 실험실에서 용출 반응조를 설치하여 20일간 배양하였으며, 퇴적물에 존재하는 물질 중 P 및 Fe와의 관계를 중점적으로 연구하였다. 분석 결과, 수층의 용존 산소는 시간의 경과에 따라 감소하는 경향을 나타냈으며, 퇴적물의 산화환원전위 또한 음의 방향으로 진행되어 혐기적 환원환경이 조성되었다. 퇴적물 산소요구량(SOD)은 배양 초기 0.05mg/g로 측정되었 으며, 20일차 0.09mg/g으로 퇴적물이 소모하는 산소량이 증가하는 경향을 관찰하였다. 이는 chl-a의 증가로 퇴적물 표층에 축적된 유기물의 분해에 의한 산소 소모(Biological-SOD), 그리고 환원반응에 의해 생성된 금속 환원물이 재산화 할 경우 소모되는 산소(Chemical-SOD)에 의한 것으로 보인다. 퇴적물에서 추출한 존재형태별 인과 SOD의 상관관계를 살펴보면 Ex-P, Org-P의 경우 양의 상관관계, Fe-P의 경우 음의 상관관계를 나타내었다. 또한, 실험 20일차 퇴적물의 미생물 군집을 분석한 결과 혐기성 철 환원균(FeRB)이 우점종으로 검출되었다. 따라서, 철 산화물과 결합한 인산염이 환원반응에 의해 분리될 경우 인산염은 수중으로 용출되어 일차생산력을 증가시키며, 환원물은 재산화 하여 퇴적물 산소 소모량에 기여하므로 본 연구는 산소 수지의 개선을 위한 기초 자료로 이용될 것으로 기대된다.

오늘날 양돈의 사양이 증가 함에 따라 부존사료 자원으로서 활용가치가 높은 물질에 항생제를 첨가 하지 않고 친환경적으로 제조된 가축용 사료를 개발하여, 기능성 친환경 돈육 및 가공품을 생산하는 것이 중요하다. 이에 따라 본 연구에서는 항암, 항염, 항산화 등 다양한 생리활성 물질이 다량 함유되어 있는 복분자(Rubus occidentalis, RO) 부산물을 양돈사료의 원료로 확립하고자 버크셔 돼지를 대상으로 복분자 발효사료(Rubus occidentalis fermented fodder, ROFF)의 혈액 내 영양 운반인자와 항산화 효능을 연구하기 위해 수행하였다. 시험구는 일반사료(대조구)에 ROFF를 0.3% 첨가하였고, 발효사료의 효능을 확인 하기 위해 각 체중에 따른 버크셔 거세돈 및 암퇘지에게 43~73일간 급여하여 효능을 확인 하였다. ROFF의 복합적인 효능은 혈액 생화학, 철분 및 항산화 분석을 통해 확인하였다. 그 결과 ROFF의 섭취로 인해 거세 자돈 및 육성돈, 암컷 자돈, 육성돈, 및 110-150 kg 암컷 비육돈 에서 total cholesterol (TC), LDL-cholesterol (LDL-C), HDL-cholesterol (HDL-C) 모두 감소하거나 HDL-C이 증가하는 경향으로 보아 영양생리학적으로 개선되는 경향을 확인하였다. 암컷 비육돈에서는 생화학적 수치가 모두 증가하는 경향으로 보아 임신 가능성이 있는 비육기의 경우 이러한 수치 향상으로 자돈의 생산을 위한 원활한 영양 공급이 예상되며, 건강한 자돈의 생산에 도움이 될 것으로 판단된다. Transferrin (TFE) 함량은 거세돈에서는 변화가 크지 않았으며, 암컷 육성돈 및 110-150 kg 암컷 비육돈에서 ROFF 섭취로 인해 증가하는 경향이 나타남에 따라 ROFF가 철분 결핍으로 인한 부정적인 영향들을 최소화 할 수 있을 것이다. Glutathione peroxidase1 (GPx1)분석 결과 거세 비육 돈 및 110-150 kg 암컷 비육돈에서 ROFF의 섭취로 인해 GPx1 활성이 유의적이지는 않지만 크게 증 가한 것으로 보아 ROFF가 항산화능을 향상시키는 것으로 판단된다. 본 연구의 결과들을 종합할 때, ROFF의 급여가 전체적으로 시험 개체의 개선 효능에는 영향이 크지 않으나 RO의 함량을 고려 할 때 매우 긍정적인 원료사료 중 하나라 할 수 있으며, 특히 ROFF가 거세돈 보다는 암컷 버크셔의 영양 운 반 및 철분 함량 등에 긍정적인 영향을 줄 수 있을 것으로 판단된다.