This study was conducted to determine the micro-hotspot for bird habitats in Yeongheung Island. We analyzed the spatio-temporal changes in the distribution based on species diversity, species richness at the 13 focal areas, which was classified in five categories depending on the types of habitats, using Analysis of Variance Test for four years (2006~2009). The distribution of birds was different depending on areas, seasons but not years. The forests of two areas of 13 areas were determined as the micro-hospot in three season (spring, summer, autumn), which is consistent for 4 years. This study provides the new analytical method that habitats for birds are systematically characterized through micro-hotspot using the spatio-temporal analysis.