'Fuyu' (Diospyros kaki L.) is an important sweet persimmon cultivar, and the fruits are often stored in a modified atmosphere after harvesting in South Korea. However, blossom-end browning and darkening of fruit often occur after harvest or during storage, which decreases fruit quality in the fresh fruit market. High fruit calcium concentration would reduce oxidation of phenolic compounds in the cytoplasm such oxidation is responsible for fruit browning. This study investigated the effects of soluble calcium fertilization and foliar application, and indole-3-butyric acid (IBA) fertilization on fruit quality and browning. Trees received one of the following five treatments: 1) control (no calcium or IBA); 2) calcium fertilization (Ca FG, 2 mL per tree); 3) calcium foliar application (Ca FA, 2 mL); 4) calcium and IBA fertilization (Ca+IBA) 5) IBA fertilization (IBA, 2 mL. Fruit calcium concentration was highest in trees treated by Ca FA, and lowest in control trees. Generally, fruit calcium concentration was high in the stem end but low in the blossom end, which usually first develops fruit-browning symptoms. There were no apparent differences in fruit qualities such as firmness, soluble solid content, and weight among treatments. Fruit browning occurred at frequencies of about 14%, 20%, and 50% on Ca FA, Ca FG, and control trees, respectively. Therefore, the improved fruit calcium level seen when trees received Ca or IBA application tended to prevent fruit browning, which increased fruit quality and storage properties.

3-weeks old Commelina was transferred to and grown in Hoagland solution (Control, 100μM Cd2+, 100μM Cd2++100μM IAA, 100μM Cd2++100μM IAA+2 mM sucrose) for 3 weeks and then the effects of indole acetic acid (IAA) on the accumulation of Cd2+ and growth of Cd2+-treated Commelina were investigated. In the treatment of Cd2+, Cd2+ was uptaked to 1.74, and 51.36 μg/g frwt. at the first week, but for three weeks, 0.51 and 34.53 μg/g frwt. in leaf and stem respectively. When IAA was treated along with Cd2+, Cd2+ was uptaked to 0.18 and 8.63 μg/g frwt. at the first week, and for the incubation of 3 weeks, 0.51 and 45.0 μg/g frwt. in leaf and stem. In case of Cd2++IAA+sucrose, Cd2+ was uptaked to 1.45 and 18.33 μg/g frwt. at the first week, but for 3 weeks, 0.51 and 25.45 μg/g frwt. in leaf and stem. Likewise Cd2+ uptake, the growth was also affected by Cd2+ and IAA. During the incubation of 3 weeks, Cd2+ reduced the stem growth about 8% in all weeks, but the treatment of IAA recovered the inhibition of stem growth caused by Cd2+ to the degree of the control Therefore, it could be concluded that IAA altered the pattern of Cd2+ uptake and the growth which were supposed to change Cd2+ toxicity.

본 실험은 쪽 세포의 현탁배양에서 indirubin 생산을 위한 indole의 처리 시기와 처리 기간 및 첨가량을 찾기 위하여 수행하였으며 얻어진 결과는 다음과 같다. 1. Indole 첨가시 세포 생장량은 억제되었다. 2. Tryptophan 첨가시 세포 생장량의 증가는 크지 않았다. 3. Indole과 L-tryptophan을 고체레지에 첨가한 경우 indole을 첨가한 배지에서 만 indirubin이 검출되었다. 4. Indirubin 생산에 적절한 indole 첨가 농도는 고체배지에서 200mg으로 나타났다. 5. 현탁배양 중 indole 첨가시기를 달리한 결과 배양 20일 후 indole을 첨가한 배지에서 세포와 배지내 indirubin 농도가 높았다. 6. 고체배양보다 현탁배양에서 세포내에 축적된indirubin이 많았으며 배지 내 로 상당량의 indirubin이 유출되었다.

IAA에 대한 단크론 항체를 생산하고 이를 이용하여 생체중의 내생 IAA를 정량분석하기 위해 ELISA를 개발하고 본법을 사용하여 담배 종자 발아중 내생 IAA함량을 정량분석하였다. 그 결과는 1. IAA에 대한 단크론 항체 생산 세포주 3가지를 선발 작성하였으며 이 세포주로부터 생산되는 항체는 모두 IgG1 타입의 면역 글로블린이었다. 2. 상기 항체를 사용하여 ELISA를 수행하여 표준곡선을 작성하였던 바 검출 한계는 1pmol이었으며 검출 범위는 500pmol이었다 3. 표준곡선으로부터 작성한 Scatchard plot에 의한 친화 상수와 결합상수는 6.7 1010 L/M과 6 1010 L/M이었다. 4. 여러가지 IAA유사물질과 교차반응에 의해서 본 mAb는 특이성이 매우 높고 RIA에 의해서 고역가임을 확인하였다 5. 발아중인 담배종자로부터 면역측정에 의해서 내생 IAA를 정량분석하였다 6. 상기의 결과에 따라서 본 mAb를 이용하여 생체중의 내생 IAA를 간편하게 정밀 분석할 수 있음을 확인하였다.