Previously we have succeeded to isolate stem cells (HEAC) from human eyelid adipose tissue, and functionally differentiate them into insulin-secreting cells. In the present study, we examined whether insulin family members might affect the insulinogenic differentiation of HEAC. Insulin treatment during culture affected little on the insulin and c-peptide secretions from HEAC after culture. However, insulin-like growth factor (IGF) 1 treatment decreased both secretions, whereas IGF2 greatly increased the secretions in a glucose-dependent manner. HEAC treated with IGF2 showed stronger expression of Pdx1, Isl1, Pax6 and PC1/3 genes compared to the control. They also showed distinct staining with insulin and c-peptide antibodies, and dithizone. While insulin or IGF2 treatment increased total cell number by 1.3- or 1.5-fold, respectively, each treatment increased the amount of insulin secretion by 27.1- or 78.1-fold, respectively. IGF2-enhanced insulinogenic differentiation was completely blocked by an antibody against insulin receptor (IR), but not by an antibody against IGF1 receptor (IGF1R). Differentiated HEAC showed expression of both IR and IGF1R genes while they expressed neither IGF2 nor IGF2R genes. Based upon these results, it is suggested that whereas IGF1 might inhibit the insulinogenic differentiation of HEAC, insulin and IGF2 could enhance the differentiation, and that the enhancing effect could be mediated via IR.
Insulin과 tumor necrosis factor alpha(TNF-)에 의한 초기 배아 발생의 조절기작을 알아보고자 생쥐의 상실배를 대상으로 이들이 첨가된 배양액에서 형태발생, 세포증식을 조사하고, 포배에서 mitogen activated protein kinase(MAPK, ERK1/2)의 활성 변화에 미치는 영향을 조사하였다. Insulin은 상실배의 체외발생 및 포배내 할구 수를 대조군에 비해 유의하게 증가시켰으며, TNF-는 발생율을 유의
생쥐 착상전 초기배아에서 insulin과 tumor necrosis factor (TNF)에 의한 배아의 형태 발생, 세포증식, apoptosis 및 MAPK활성의 변화를 조사하였다. Insulin에 의해 형태발생 및 포배당 세포수가 증가되었으며 TNF 처리시 유의하게 감소하였다. TNT 전처리시 insulin에 의한 발생 및 세포수 증가 촉진효과가 상쇄되었으며 TNF는 배아내 caspase-3의 활성을 증가시켰다. Insulin은 단시간내에 포배에