The present study was conducted to investigate the effect of BHT supplementation on sperm motility, viability, acrosomal integrity and plasma membrane integrity after frozen-thawing. One ejaculate was collected from one fertile Hanwoo bull by using artificial vagina at Hanwoo Research Institute. The ejaculate was transferred to laboratory immediately and diluted with pre-warmed semen extender (Optixcell, France) (1:1). Sperm dilutions were extended to a final concentration of 40 x 106 sperm/ml, and divided into 5 groups according to BHT concentration (0, 0.5, 1.0, 2.0 and 4.0 mM) and cryopreserved in LN2 tank until evaluation. Frozen-thawed semen was transferred to 1.5 ml tube and incubated for 0, 2 and 4h. Sperm motility and motility parameters (total motility, VSL with 25μm≥, VCL, VSL, VAP, LIN, STR, WOB, ALH and BCF) were evaluated by sperm class analysis (SCA, IVOS, Spain). There were not significant effects of BHT supplementation (0, 0.5, 1.0 and 2.0 mM) on total motile and VSL with 25μm≥ at 0, 2 and 4h. However, 4.0 mM of BHT supplementation showed negative effect on total motile (26.3%), VSL with 25μm≥ (1.3%) at 0 h (p<0.001). The viability and acrosomal integrity of spermatozoa were evaluated by Trypanblue/Giemsa staining method and divided into 4 groups; live and intact acrosome (LIA), live and damaged acrosome (LDA), dead intact acrosome integrity (DIA), dead damaged acrosome (DDA). There were no significant differences of LIA, LDA, DIA and DDA on various BHT concentrations at 0 and 2 h. However, 4.0 mM BHT supplementation showed decreased LIA compared with 0, 0.5, 1.0 and 2.0 mM BHT at 4 h (34.6, 37.1, 43.6, 45.4 and 14.7% vs. 0, 0.5, 1.0, 2.0 and 4.0 mM, irrespectively; p<0.01). Addition of 4.0 mM of BHT showed negative effect on plasma membrane integrity compared with that of 0, 0.5, 1.0 and 2.0 BHT at 2 h (71.9, 64.2, 64.6, 67.5 and 31.7 % vs. 0, 0.5, 1.0, 2.0 and 4.0 mM, irrespectively; p<0.05). In conclusion, various BHT concentrations on optixcell extender showed no improvement on sperm motility, viability and plasma membrane integrity.

The purpose of this study is to evaluate the effects of astaxanthin added to freezing buffer on semen parameters, total sperm oxidation stress after post-thawing of boar sperm and lipid peroxidation (LPO) which is caused by reactive oxygen species (ROS) in sperm membrane. Varying concentrations of astaxanthin (0, 10, 50, 100 and 500 μM) were used in the freezing buffer during cryopreservation to protect the DNA of thawed miniature pig sperm. Semen parameter was measured using computer assisted sperm analysis (CASA) for sperm motility and determine ROS rate, oxidative stress of boar sperm using fluorescence-activated cell sorting (FACS). Sperm motility was higher (p<0.05) in the astaxanthin group than in the control group. Sperm motility and the number of progressive motile sperm was higher (p<0.05) in the astaxanthin 500 μM group (66±1.7%) than in the control group (49.8±4%). In ROS evaluation, the astaxanthin group lowered intracellular O2 and H2O2 in viable sperm. The Yo-Pro-I/HE and PI/H2DCFDA staining as revealed using flow cytometry was lower in astaxanthin groups than in the other groups. As the result, we found that astaxanthin could protect the sperm plasma membrane from free radical and LPO during boar sperm post-thawing.

This study evaluated the mechanical joining characteristics of substrate Al7075 using the filler metals of ER5356 and Al7075 to secure the joining integrity of the specimens by GTAW. The results of radiographic test show that the welded specimens meet the first level standard of KS D 2042. Besides, welding defects were not occurred. The tensile strengths of the specimens using the filler metals of Al7075 and ER5356 had 240MPa and 252MPa, respectively. The yield strengths were 132MPa and 120MPa, respectively. In case of using the filler metal of Al7075, However, in case of using the filler metal of ER5356, Two filler metals of Al7075 and ER5356 were similar to each other in tensile and yield strength.

The purpose of this study was to examine the effects of taurine and vitamin E on ovarian granulosa cells damaged by bromopropane (BP) in pigs. We evaluated cell viability, plasma membrane integrity (PMI) and apoptotic morphological change in porcine ovarian granulosa cells. The cells were treated with 1-BP (0, 5.0, 10, and 50 μM), 2-BP (0, 5.0, 10, and 50 mM), taurine (0, 5.0, 10, and 25 mM), and vitamin E (0, 100, 200, and 400 μM) for 24 h. 10 μM 1-BP and 50 μM 2-BP inhibited viability and PMI, and induced apoptosis in porcine ovarian granulosa cells (p < 0.05). Cell viability and PMI were increased by taurine (10 and 25 mM) and vitamin E (100 and 200 μM), and apoptosis decreased (p < 0.05). Finally, the porcine ovarian granulosa cells were co-treated with BPs (10 μM), taurine (10 mM) and/or vitamin E (200 μM). Cell viability and PMI in the co-treated cells were increased, and apoptosis was decreased. In conclusion, taurine and vitamin E can improve cell viability and inhibition of apoptosis in porcine ovarian granulosa cells damaged by bromopropane.

가스 터빈은 기동 및 정지 횟수가 많기 때문에 열피로나 취화 현상으로 인한 가스터빈 케이싱의 균열 또는 케이싱의 플랜지면에서 고온고압 가스의 누설이 발생할 가능성이 높다. 따라서 가스터빈 케이싱의 구조안전성 및 플랜지면에서의 누설평가는 반드시 수행되어야 하는 부분이다. 본 논문에서는 유한요소해석을 바탕으로 터빈 케이싱의 ASME B&PVC VIII-2 구조안전성 평가 및 접촉압력을 통한 누설 평가 그리고 볼트의 구조안전성 평가를 진행하였다. 또한 가스터빈 케이싱의 유한요소모델링 및 해석/평가 방법을 제안하여 가스터빈 개발에 활용할 수 있게 하였다.

가스 터빈은 기동 및 정지 횟수가 많기 때문에 열피로나 취화 현상으로 인한 가스터빈 케이싱의 균열 또는 케이싱의 플랜 지면에서 고온고압 가스의 누설이 발생할 가능성이 높다. 따라서 가스터빈 케이싱의 구조안전성 및 플랜지면에서의 누설평 가는 반드시 수행되어야 하는 부분이다. 본 논문에서는 유한요소해석을 바탕으로 터빈 케이싱의 ASME B&PVC VIII-2 구 조안전성 평가 및 접촉압력을 통한 누설 평가 그리고 볼트의 구조안전성 평가를 진행하였다. 또한 가스터빈 케이싱의 유한 요소모델링 및 해석/평가 방법을 제안하여 가스터빈 개발에 활용할 수 있게 하였다.

Trusting beliefs are playing an increasingly important role in building customer-based brand equity (CBBE) in the business arena. The present study examines the mediating effect of trusting beliefs on the interactivity-brand equity relationship. An online survey was administered to collect data from randomly selected consumers. The results show that system interactivity impact brand equity directly and indirectly through trust integrity. The present theory-driven model contributes to a better understanding of online brand equity. Therefore, allocating resources to influence the perception of system interactivity and trusting integrity beliefs is valuable to develop online brand equity. This research contributes to the interactivity theory and the relationship marketing theory and it also offers implications for practitioners.

The purpose of this study was to examine the effects of taurine and vitamin E on sperm characteristics damaged by bromopropane (BP) in pig. We evaluated toxicity of BP on viability, membrane integrity and mitochondrial activity of spermatozoa. 1-BP (0, 2.5, 5.0, 10, and 50 μM), 2-BP (0, 2.5, 5.0, 10, and 50 μM), taurine (0, 5.0, 10, and 25 μM) and vitamin E (0, 50, 100, and 200 μM) were treated in fresh boar semen for 6 h. 10 and 50 μM of 1-BP and 2-BP inhibited sperm viability, membrane integrity and mitochondrial activity in fresh boar semen (P<0.05). 25 μM of taurine increased sperm viability and membrane integrity (P<0.05), 100 μM of vitamin E enhanced viability and mitochondrial activity of sperm (P<0.05). Finally, 10 μM of 1-BP and 2-BP was co-treated with taurine (25 μM) and vitamin E (100 μM) in the fresh boar semen. The co-treated samples did affected viability, membrane integrity and mitochondrial activity of sperm. In conclusion, taurine and vitamin E can improve and maintain sperm quality in fresh boar semen.

In this paper, as the transport cask was moved in the reactor, the structural integrity on the cask had to be evaluated in the normal transport condition. The drop height of the cask was determined by the weight of the cask in the normal transport condition by regulations about assessment test. It was determined that the drop height of the cask was 1.2 m by regulations. The velocity of the drop impact was calculated to perform the drop impact analysis by the principle of the conservation of energy. Using results of the simulation about the drop impact analysis, the structural integrity assessment on the transport cask was performed by ASME Boiler and Pressure Vessel Code.

원자로 격납건물은 냉각재상실사고와 같이 내부의 과도한 압력이 유발되는 사고에 있어서도 방사성 물질이 외부로 누출되지 않도록 막는 최종의 방벽이다. 이러한 격납건물의 기능적 중요성에 기인하여, 건설 초기 구조건전성시험(SIT)을 수행한다. 이러한 SIT거동을 가장 실제와 가깝게 예측하기 위한 해석 연구를 수행하였다. 해당 연구의 결과는 2편의 논문으로 정리되었는데, 본 논문은 그 중 II편으로 I편의 해석모델 구성 시의 주요 고려사항의 분석 및 예비해석 결과를 반영한 상세 해석 모델의 구성 과정 및 해석 결과를 제시하고 있다. 특히 비부착식 텐던으로 시공된 구조물에서 덕트관에 의한 강성 저감효과 및 덕트관을 사이에 둔 텐던과 콘크리트간의 밀착 여부에 따른 영향을 해석 시 최대한 고려하고자 하였다. 이러한 과정을 통해 구축된 해석 모델에 따른 변위과 신고리 3호기 SIT 측정변위를 비교한 결과, ASME CC-6000 기준을 충분히 만족시키는 결과가 나타남을 확인하였다.

원자로 격납건물은 냉각재상실사고와 같이 내부의 과도한 압력이 유발되는 사고에 있어서도 방사성 물질이 외부로 누출되지 않도록 막는 최종의 방벽이다. 이러한 격납건물의 기능적 중요성에 기인하여, 건설 초기 구조건전성시험(SIT)을 수행한다. 신고리 3호기 SIT 시험 당시 계측된 변위를 예측하기 위한 초기 해석 모델은 일부 위치에서 실제 변위를 과소 평가하는 경향을 보임에 따라 이를 개선하고자 하는 연구가 수행되었다. 해당 연구의 결과를 I 편과 II 편의 논문으로 정리하였으며, 본 I 편에서는 초기 해석모델을 개선해가는 과정에서의 해석모델 구성 시의 주요 고려사항의 분석 및 예비해석 결과를 제시하고 있다. 우선적으로 콘크리트 자체의 해석요소(mesh) 구성과 라이너, 철근, 텐던 등의 요소간의 연결 설정이 중요함을 확인하였다. 또한, 다양한 예비해석의 결과를 통해 비부착식 텐던으로 시공된 구조물에서 덕트관에 의한 강성 저감 효과 및 덕트관을 사이에 둔 텐던과 콘크리트간의 밀착 여부에 따른 강성 영향을 적절히 고려하는 것이 중요함을 확인하였다.

공무원 청렴도는 국가 브랜드 향상 및 경쟁력 강화에 중요한 핵심요인이며 사회적 자본형성에도 기여한다. 그러나 우리나라의 2014년 부패인식지수는 100점 만점중 55점으로 6년 연속 하위권에 정체되어 있고, 국내의 공공기관을 대상으로 한 청렴도평가에서 지방자치단체의 청렴도는 하위에 머물고 있다. 본 연구는 정부 및 지방자치단체가 다수의 청렴정책 발표와 감사원 징계를 강화하고 있음에도 불구하고 크게 나아지지 않고 있어 공무원의 청렴 또는 반부패에 대해 어떻게 인식하는가가 중요하다는데 출발한다. 따라서 연구목적은 경상남도 공무원 집단을 대상으로 청렴도 인식을 분석하고 정책적 시사점 도출을 통해 청렴도 향상방안을 제시한다. 분석결과, 공무원의 청렴도 및 청렴도 노력 정도가 상대적으로 높게 인식하였고, 행정분야별 청렴도는 소방, 토지, 환경이었으며, 건축·건설·주택은 매우 낮게 인식하였다. 부패인식에서 부패계층으로 국·과장 (46.5%)이 가장 높고, 부패업무분야는 공사발주 분야(45.4%)가 많으며, 부패원인으로 공무원 부패행위에 대한 관대한 처벌(30.6%), 부패유형은 직위를 이용한 알선이나 청탁(35.7%)로 높게 인식하였다. 청렴정책은 인지하는 정책으로 반부패청렴교육(79.4%), 효과가 큰 청렴정책은 원-스트라이크아웃제(28.9%), 부정 인지시 대처방법은 내부고발시스템 이용(23.0%)으로 나타났다. 이러한 결과가 주는 정책적 시사점으로 첫째, 부패에 대한 공무원의 관대한 인식, 둘째 공무원 청렴교육에 대한 전면적 재검토, 셋째, 익명성보장내부고발제도의 효율적 활용방안 마련을 제시하였다.

As the world energy consumption grows, the interest in marin energy resources is increasing. In excavating such resources, the marine riser which connects the floating structure and sea bed is an essential device. The riser system is often exposed to harsh ocean environment and thus vulnerable to damage. Since the failure of the riser system may cause serious economical loss as well as environmental problem, the structural integrity of the riser is very important. Generally, the riser is an extremely slender structure with a much smaller diameter than a length. Therefore, a structural integrity monitoring methodology for typical buildings and bridges may not be applicable. In this paper, the applicability of a damage identification method for a structure to a marine riser is examined via a numerical example. Also, recent research practices and findings for monitoring the behavior and the structural integrity of the marine riser are examined and summarized.

본 논문에서는 노심용융사고 시 관통노즐이 제거된 원자로용기 하부헤드의 구조 건전성 평가를 수행하였다. 열응력, 노심용융물의 질량 그리고 내압조건의 해석결과를 고려할 때, 하부헤드의 열응력에 의한 영향이 가장 크게 나타났다. 손상 가능성은 파손기준에 따라 평가하였으며, 등가소성변형률이 임계변형률 파손기준보다 낮은 수준으로 평가되었다. 열-구조물 연성해석 결과 하부헤드의 두께 중간층에서 항복강도보다 낮은 응력이 발생한 탄성영역 구간을 확인하였다. 내압이 커지면서 탄성영역 범위가 점차 좁아지면서 탄성영역이 내벽으로 이동하는 결과를 확인하였고, 노심용융사고 시 구조적 건전성을 만족하는 것으로 평가되었다.

The objective of this study was to compare the effect of semen extenders on the sperm motility, viability, acrosome integrity and functional integrity of plasma membrane (HOST: hypo-osmotic swelling test) during liquid preservation of Korean Native boar semen. In this experiment, semen was diluted in Androhep plus, Beltsville Thawing Solution (BTS), ModenaTM, Seminark and Vitasem LD. Sperm-rich fractions were collected from three Korean Native boars and sub-samples were diluted (30×106 spermatozoa/ml) in different semen extenders. Semen samples were stored at 17℃ for 96 hours. On everyday (0, 24, 48, 72, 96 h) after storage, the sperm characteristics relevant for fertility, such as sperm motility, viability, acrosome integrity and HOST positive were evaluated. The motility of spermatozoa stored in different extenders was no significantly different among other extenders (P>0.05). Also, no difference was observed among samples processed with different extenders in the percentage of sperm viability, acrosome integrity and HOST positive. All extenders maintained a high percentage (70%) of sperm motility, viability and acrosome integrity through 96 h of storage. The result of this study show that there was no significant differences among extenders in their capacity to preserve motility, viability and membrane integrity of spermatozoa from normal, fertile Korean Native boars for 96 h of liquid preservation at 17℃.

Cryopreservation and in vitro fertilization (IVF) protocols are important in genetic studies and applications to transgenic animals. Various studies about boar sperm cryopreservation have been studied for a long time. Those were about the use of extenders, the choice of sugars, the cooling and warming rates. The factors that influence the boar sperm are the dramatic changes in temperatures, osmotic and toxic stresses, and reactive oxygen species (ROS) generation. Among these factors, ROS generation is the main damage to DNA which is a principal genetic material and the most important for the practical applications. So we wondered whether ROS generation could be reduced. In previous study, monothioglycerol (MTG) was essential for the culture of embryo stem cells. Therefore we added MTG in the freezing extender based on lactose-egg yolk (LEY) with trehalose. For the assessment of the frozen-thawed sperm, we focused onmotility, membrane integrity and DNA damage. First, we used a computer-aided sperm analysis system for overall conditions of sperm such as motility and viability. Then we performed the sperm chromatin structure assay for DNA integrity and hypo-osmotic swelling test for membrane integrity. And our result showed the existence of MTG in the freezing extender caused less damage to DNA and higher motility in frozen-thawed boar sperm. Also we checked a relative antioxidant activity of MTG in modified Modena B extender. We concluded that this reagent can activate sperm mitochondria at MTG 0.2 μM, contribute to sperm motility and DNA integrity but there was no significant difference on membrane integrity. Also antioxidant activity of MTG in modified Modena B extender was proved.

In this study, we used flow a cytometric assay to evaluate plasma membrane integrity and mitochondrial activity in post-thawed sperm that was supplemented with ginsenoside-Rg1. Varying concentrations of ginsenoside-Rg1 (0, 25, 50 and 100 μM/ml) were used in the extender during cryopreservation to protect the DNA of thawed sperm, thereby increasing the viability and motility rate as evaluated using a computer-assisted sperm analysis (CASA) method. The results derived from CASA were used to compare the fresh, control, and ginsenoside-Rg1 groups. Sperm motility and the number of progressively motile sperm were significantly (p<0.05) higher in the 50 μM/ml ginsenoside-Rg1 group (61.0±4.65%) than in the control (46.6±7.02%), 25 μM/ml (46.2±4.76%), and 100 μM/ml ginsenoside-Rg1 (52.0± 1.90%) groups. However, the velocity distribution of post-thawed sperm did not differ significantly. Membrane integrity and MMP staining as revealed using flow cytometry were significantly (p<0.05) higher (91.6±0.82%) in the 50 μM/ml ginsenoside-Rg1 group than in the other groups. Here, we report that ginsenoside-Rg1 affects the motility and viability of boar spermatozoa. Moreover, ginsenoside- Rg1 can be used as a protective additive for the suppression of intracellular mitochondrial oxidative stress caused by cryopreservation.

This study was designed to determine whether low-density lipoporoteins (LDL) extracted from egg yolk in extender improve the function of Korean Jeju Black Bull semen. The semen was cryopreserved with 5% ethylene glycol (EG) or 7% glycerol (G) extenders containing 10% egg yolk (EY), 4% LDL and 5% EY or 8% LDL. Frozen-thawed sperm were evaluated sperm motility, viability, membrane integrity and acrosome integrity. Post-thawed sperm motility has been significantly higher (p<0.05) in 4% LDL + 5% EY (; EG and ; 7% G) than 8% LDL (; EG and ;G). Treatment of 4% LDL + 5% EY-EG () has been significantly improved sperm viability compared to other treatments except 10% EY - EG. Moreover, in membrane integrity, swollen sperm ratio has been only significantly increased (p<0.05) in 4% LDL + 5% EY - EG () among all treatments. In assess to detect acrosome integrity, especially, AR pattern ratio has been significantly decreased (p<0.05) in 4% LDL + 5% EY - EG among all treatments. In sperm viability as time passes, between 4% LDL + 5% EY and 10% EY, there was no significant difference, but 8% LDL was significantly decreased sperm viability in EG (1 and 2 hrs) and G (30 min, 1, 2, 5 and 12 hrs) extender. However, there were no significant differences among all treatments except 8% LDL-G in sperm membrane integrity. 8% LDL-G has been significantly decreased swollen sperm ratio at 5 hrs after thawed. It is concluded from these results that 4% LDL + 5% EY to the freezing extender showed more positive effect on the frozen-thawed spermatozoa in Korean Jeju Black bull.