Periodically, the Japanese Castle was created in the domestic of Japan and then 2 invasions into Chosun was started. The Japanese Castle in the domestic of Japan was repaired several times by the building boom of castle before & after 2 invasions and so the initially-built type of castles was changed. Accordingly, there are much difficulties to understand the original shape of Japanese Castle. Through the Japanese Castle within Korea called as the fossil of Japanese Castle, I would like to examine & consider its building period and characteristics. The terminology called as [Two Side Stone's Wall] is that of castle which is not acknowledged in the Japanese Academic Circles. However, it means the two-fold wall of Japanese Castle which was widely applied to the fortification way in the Age of Japan Edo. The terminology of [Sori] says the stonework curve in the corner of Japanese Castle which is indicated best in the Japanese Castle. It calls the curve as like the fan frame. [Curb Stone's Wall] says the type of castle wall constructed with over 1 face in wall body of Japanese Castle. (1) About classifying the construction period of Japanese Castle, the curb stone's wall and the castle having no two side stone's wall must consider the building period as that of Japanese Invasion of Korea in 1592. If there was [Sori], the two-side stone's wall was used and the place which supported the documentary data, in particular, the place having the record of contraction is considered to be confirmed as the castle constructed in the period of Japanese Invasion of Korea in 1597. (2) The two-side-type stone's wall shown in the Modern Japanese Stone Castle is difficultly considered to be generated from the Japanese Castle at the period of Japanese Invasion of Korea in 1592 and in 1597. (3) The beautiful [Sori] shown in the Modern Japanese Stone Castle was started from the Japanese Castle of Korea at the period of Japanese Invasion of Korea in 1597. It is difficultly considered which its indication was firstly generated by the Chaesung-Folded Segment Structure.

Periodontal disease, a form of chronic inflammatory bacterial infectious disease, is known to be a risk factor for cardiovascular disease (CVD). Porphyromonas gingivalis has been implicated in periodontal disease and widely studied for its role in the pathogenesis of CVD. A previous study demonstrating that periodontopathic P. gingivalis is involved in CVD showed that invasion of endothelial cells by the bacterium is accompanied by an increase in cytokine production, which may result in vascular atherosclerotic changes. The present study was performed in order to further elucidate the role of P. gingivalis in the process of atherosclerosis and CVD. For this purpose, invasion of human aortic smooth muscle cells (HASMC) by P. gingivalis 381 and its isogenic mutants of KDP150 (fimA⁻), CW120 (ppk⁻) and KS7 (relA⁻) was assessed using a metronidazole protection assay. Wild type P. gingivalis invaded HASMCs with an efficiency of 0.12%. In contrast, KDP150 failed to demonstrate any invasive ability. CW120 and KS7 showed relatively higher invasion efficiencies, but results for these variants were still negligible when compared to the wild type invasiveness. These results suggest that fimbriae are required for invasion and that energy metabolism in association with regulatory genes involved in stress and stringent response may also be important for this process. ELISA assays revealed that the invasive P. gingivalis 381 increased production of the proinflammatory cytokine interleukin (IL)-1β and the chemotactic cytokines (chemokine) IL (interleukin)-8 and monocyte chemotactic (MCP) protein-1 during the 30-90 min incubation periods (P<0.05). Expression of RANTES (regulation upon activation, normal T cell expressed and secreted) and Toll-like receptor (TLR)-4, a pattern recognition receptor (PRR), was increased in HASMCs infected with P. gingivalis 381 by RT-PCR analysis. P. gingivalis infection did not alter interferon--inducible protein-10 expression in HASMCs. HASMC nonspecific necrosis and apoptotic cell death were measured by lactate dehydrogenase (LDH) and caspase activity assays, respectively. LDH release from HASMCs and HAMC caspase activity were significantly higher after a 90 min incubation with P. gingivalis 381. Taken together, P. gingivalis invasion of HASMCs induces inflammatory cytokine production, apoptotic cell death, and expression of TLR-4, a PRR which may react with the bacterial molecules and induce the expression of the chemokines IL-8, MCP-1 and RANTES. Overall, these results suggest that invasive P. gingivalis may participate in the pathogenesis of atherosclerosis, leading to CVD.

Tropical fire ant, Solenopsis geminata (Fabricius) is distributed over the tropical and temperate areas. It is a polyphagous pest attacking to various plant and causes the pain to the people doing outdoor work. So it is known for a hygienic pest to human being, especially the people doing outdoor activities. It can cause severe pain to human, even with just a bite. Of particular concern is the possibility of its settlement in this country, if it invade to the farmlands and/or forests of Jeju Island and southern part in Korea. It is listed as a regulated pest in Plant Quarantine Act of Korea. This ant is usually imported through a ship and early settled in the port area where it is already distributed. Recently its distribution is rapidly spreading into Southeast Asia and southern part of China. In Korea, it was detected the one time in 1990s, but was detected the ten times from 2000 to 2006 by an imported plant quarantine process. Therefore, we would like to provide some information on the species, including morphology, distribution, host plant, damaged symptom and its general bionomics, which will be useful data for plant quarantine process and control.

Oral squamous cel1 carcinoma(OSCC) is the most common malignancy of head neck region. Typically OSCC cells s how persist ent invasion that frequently leads to local recurrence and distant lymphatic metastasls However, molecular mechanisms of invasion of OSCC remain poorly understood. Her e we identifi ed periostin, interferon induced transmembrane protein l (IF1TM1) and wingless-type MMTV integration site family, member 5B(WNT5B) , as invasion promoting molecules in OSCC by comparing gene expression profiles between a parent OSCC cell line(MSCC-l) and its highly invasive clone(MSCC-1nvl). Overexpression of periostin, IFITMl and WNT5B mRNAs were confirmed in MSCC-1nvl by RT-PCR. Transfection of these molecules promoted invasion of OSCC cells Moreover , siRNA t r eatment of these molecules suppressed invasion of cancer cells in vitro I nter estingly, Periostin, 1F1TMl and WNT5B were highly expressed in OSCCs in comparison with nonnal tissues. 1n an orthotopic mouse model of OSCC, periostin-overexpressing cells metas tasized spontaneously to cervical lymph nodes and t o t he lung through their aggressive invasiveness. These findings suggest that peri ostin, IFITMl and WNT5B play important roles for invasion and of OSCC and can be prognostic markers and therapeutic t argets of OSCC.

본 시험은 제주지역에서 지렁이분 시비량 차이(0, 150, 300, 450, 600kg/10a)에 따른 한지형 잔디(Creeping bentgrass)의 식생의 변화를 검토하고, 지렁이분 적정시비량을 구명하기 위하여 2004년 3월 21일부터 7월 10일까지 시험하였다. 초장은 무비구에서 지렁이분 600kg/10a까지 16.7cm에서 20.2cm로 커졌으나, 지렁이분 450kg과 600kg/10a 시비구에서는 유의한 차이가 없었다. 근장, 엽록소는 초장

Alterations in cell surface receptors and adhesion molecules which regulate cell-cell and cell-matrix interactions have been 뻐plicated in tumor processes. In order to investigate the effect of integrin expression on the invasiveness of oral sqllamous cell carcinoma, integ띠1 expression in the celllines such as SCC-4, SCC-9, SCC-15, and SCC-25 was analyzed, and the comparison between cell adhesion assay towards extracellular matrix proteins and in vitro invasion assay following inhibition of the functional domain of the integrins using blocking antibodies against the specific integrins 낀 nd Arg-Gly-Asp (RGD) peptide were carried out. The expression of integrin a 2, a 3, a 6 was detected in all oral squamous cell carcinoma celllines. In contrast, the expression of a vß6 integrin is detected in SCC-4 and SCC-9, not in SCC-1 5 and SCC-25. 까1e adhesion of SCC-4 cell line to collagen 1, laminin, and fibronectin was significantly reducecl by σeatment with a 3-, a 6-, and a vß6-blocking antibody, respectively (p (0.05). 꺼.1e invasion of SCC-4 cell line throllgh Matrigel was significantly reduced by treatment with v 6-blocking antibody and RGD pepticle (p(0.05). These results sllggested that specifìc integrins play an in1portant role in the process of adhesion and invasion of oral squamous cell carcinoma cells and the expression of a vß6 integrin is believed to the enhance its invasivene잃.

지속가능한 빗물정원 설계를 위해서는 내부 구성요소 (식물, 토양, 여재층 등)의 최적화된 상호작용에 의하여 나타나는데 특히 국내 기후 환경에서는 함수량의 변동이 심하며 적용 가능한 식물의 종이 선정이 중요하다. 지속적 빗물정원으로서의 효율은 빗물의 유입패턴에 따른 적용가능한 식물과 여재의 종류에 의해 결정되고, 식생의 생장 차이에 따른 식재공간 유지 정도에 의해 지속성이 좌우된다. 본 연구는 근부 생장이 활발한 습생식물의 정원용 소재로서의 평가를 위해 식물별 침입수준(invasion level)을 분석하여 최적의 식재조합을 설계하는데 기 초자료로 활용하고자 한다. 대상식물은 속새 외 19종의 습생식물을 관상기준에 따라 직립형, 분지형, 포복형으로 나누고 5월부터 10월까 지 월 2회 각 식물의 피복율을 측정하였다. 20종 중 단위공간 피복율이 100%를 넘어 침입성이 높은 수준의 식물은 부처꽃, 박하, 아스타, 낙지다리, 갯까치수영, 삿갓사초, 고랭이로 나타났고 식재 초기부터 급속한 성장을 보인 식물은 갯까치수영, 박하, 낙지다리, 삿갓사초, 부 처꽃 순으로 식재설계시 고려해야할 수종으로 나타났다.

By analyzing the compressive strength througth field test execution on the shotcrete mixture with 40% blast furnace slag and slurry accelerator, confirmed the suitability as subsea tunnel material. As a result, strength characteristic on the anti-chloride shotcrete is excellent evaluated in terms of compressive strength compared with existing shotcrete.