농업 재배지 토양으로부터 auxin 생성세균 KSD16, KSD33 그리고 KSD36를 분리하였다. 분리 균주 KSD16, KSD33 그리고 KSD36 는 16S rRNA 유전자 계통분석을 통해 Arthrobacter 속으로 분류되 었다. 이들 균주는 R2A배지에 0.1% L-tryptophan를 첨가한 배지에서 28℃, 48 시간 배양한 결과, auxin 의 일종인 indole-3-acetic acid (IAA)를 204.4 mg L-1 생성하는 것으로 확인되었다. IAA 생성세균의 녹 두종자 발아에 미치는 영향을 확인한 결과, Arthrobacter 속 균주 KSD16, KSD33 그리고 KSD36 는 대조 군에 비해 뿌리길이와 발근수가 증가하였다. Arthrobacter 속 균주의 녹두 성장촉진 효과를 확인한 결과, 녹두의 발아율이 대조군보다 73.4 % 증가하는 특징을 나타내었다.

The objective of this study is to investigate the earthquake response for the design of 100m spanned single-layer lattice dome. The plastic hinge analysis and eigenvalue buckling analysis are performed to estimate the ultimate load of single-layered lattice domes under vertical loads. In order to ensure the stability of lattice domes, it is investigated for the plastic hinge progressive status by the pushover increment analysis considering the elasto-plastic connection. One of the most effective methods to reduce the earthquake response of large span domes is to install the LRB isolation system of a dome. The authors discuss the reducing effect for the earthquake dynamic response of 100m spanned single-layered lattice domes. The LRB seismic isolation system can greatly reduce the dynamic response of lattice domes for the horizontal and vertical earthquake ground motion.

ASCE 4 requires that a hard stop be built around the seismic isolation system in nuclear power plants. In order to maintain the function of the isolation system, this hard stop is required to have clearance-to-stop, which should be no less than the 90th-percentile displacements for 150% Design Basis Earthquake (DBE) shaking. Huang et al. calculated clearance-to-stop by using a Latin Hypercube Sampling technique, without considering the rocking behavior of the isolated structure. This paper investigates the effects on estimation of clearance-to-stop due to 1) rocking behavior of the isolated structure and 2) sampling technique for considering the uncertainties of isolation system. This paper explains the simplified analysis model to consider the rocking behavior of the isolated structure, and the input earthquakes recorded at Diablo Canyon in the western United States. In order to more accurately approximate the distribution tail of the horizontal displacement in the isolated structure, a modified Latin Hypercube Sampling technique is proposed, and then this technique was applied to consider the uncertainty of the isolation system. Through the use of this technique, it was found that rocking behavior has no significant effect on horizontal displacement (and thus clearance-to-stop) of the isolated structure, and the modified Latin Hypercube Sampling technique more accurately approximates the distribution tail of the horizontal displacement than the existing Latin Hypercube Sampling technique.

The objective of this study was to compare of different isolation method of mouse preantral follicles, and to examine in vitro development of mouse preantral follicles isolated by different method. Preantral follicles were mechanically or enzymatically extracted from mouse ovaries. Mechanical isolation method used fine gauge needles and enzymatic method of isolating follicles used collagenase. The recovered preantral follicles were cultured for 10 days in alpha-minimal essential medium (-MEM) + 5% FBS + Insulin-Transferrin-Selenium (ITS) + 100 mIU/ml FSH. The collected primary follicles by enzymatic treatment were higher than mechanical method. Others stage preantral follicle by mechanical isolation were higher than enzymatic method. After 10 days of culture, no statistical differences were shown in survival rates of preantral follicle among the 2 culture groups. The metaphase II rates of the oocytes were significantly higher (p<0.05) in mechanical method (17.8%) than in enzymatic method (5.1%). These results suggest that the isolation method of choice depends on the target stage preantral follicles and mechanical isolation is an optimal method of preantral folliclesin a culture of mouse preantral follicle.

Culture of preantral follicles has important biotechnological implications through its potential to produce large quantities of oocytes for embryo production and transfer. The objective of this study was to determine the comparison of different isolation method of mouse preantral follicles, and to examine in vitro development of mouse preantral follicles isolated by different method. Preantral follicles were mechanically or enzymatically extracted from mouse ovaries. Mechanical isolation method used fine gauge needles and enzymatic method of isolating follicles used 1 mg/ml collagenage (Type IA) and 0.2 mg/ml DNase Ⅰ in Leibovitz L-15 medium. The solution containing Leibovitz L-15 medium, enzyme and ovary fragments was incubated at 37℃ for 30 min. The selection criteria are as follows: primary follicle of 75 to 99 μm, early secondary follicle of 100 to 125 μm and late secondary follicle of 126 to 150 μm in diameter. The recovered preantral follicles were cultured for 10 days in alpha-minimal essential medium (α-MEM) + 5% FBS + ITS + 100 mIU/ ml FSH. The collected primary follicles by enzymatic treatment were higher than mechanical method. Others stage preantral follicle by mechanical isolation were higher than enzymatic method. After 10 days of culture, no statistical differences were shown in survival rates of preantral follicle among the 2 culture groups. The metaphase Ⅱ rates of the oocytes were significantly higher (p<0.05) in mechanical method (17.8%) than in enzymatic method (5.1%). These results suggest that the isolation method of choice depends on the target stage preantral follicles and mechanical isolation is an optimal method of preantral follicles in a culture of mouse preantral follicle.

충적지반에 건설된 원형 단면을 갖는 터널은 지진시에 지반의 전단 변형의 영향을 받아 좌우교차로 경사진 타원형상의 변형을 반복한다. 본 논문에서는 이 특별한 진동모드를 이용하여 지반-터널계의 상호작용 및 면진 효과가 검토되었다. 지반과 터널의 경계가 완전히 결합되어 있는 경우에 대한 지반 -터널계의 상호작용 효과 및 지하 구조물의 지진피해를 줄이는 한가지 방법으로써 터널 주위를 면진재로 피복하는 방법에 대한 면진효과가 토론되었다. 그 결과 면진재의 포아송비를 작게 하거나 지반과 면진재의 전단탄성계수의 비를 증가시킴으로서 면진 효가가 증가함을 알 수 있었다.

For the purpose of isolation and screening of tyrosinase inhibitory activity from edible mushrooms, Pleurotus ostreatus, Auricularia auricula judge, Umbilicaria esculenta, Agaricus bisporus, Flammuline velutipes, Lentinus edodes, Ganoderma lucidum, and Coriolus uersicolor were examined by tracing inhibitory activities against tyrosinase, utilizing L-3,4-dihydroxyphenylalanine (L-DOPA) as a substrate. Among the eight edible mushrooms tested, Umbilicaria esculenta showed potent enzyme inhibitory activities above 78.4% against tyrosinase in ethylacetate (EtOAc) extracts. Ganoderma lucidum and Agaricus bisporus showed inhibitory activities of 67.3% and 51.5% in water extracts. EtOAc extracts of Umbilicaria esculenta was fractionated from silicagel column chromatography and one fraction showed the most inhibitory activity of 60.9%. The three bands (Rf=0.38, 0.27, 0.19) were isolated from preparative TLC of the fraction for purification and identified as mixtures of orsellinate, methyl orsellinate, methyl lecanorate, and methyl gyrophorate by high pressure liquid chromatography (HPLC), ultravisible spectrophotometer (UV), mass spectrophotometer (Mass), nuclear magnetic resonance spectrometer (NMR).

목적: 베이비붐 세대의 팀 동일시, 시청몰입, 사회적 고립감 간의 구조적 관계를 규명함으로 코로나19(COVID-19) 시대에 베이비붐 세대가 겪고 있는 사회적 고립감 문제를 해결할 수 있는 구체적 방향을 제시하고, 향후 또 다른 고립의 상황에 대비할 수 있도록 돕는 것에 본 연구의 목적이 있다. 방법: 본 연구의 목적을 달성하기 위해, 수도권에 거주하는 302명의 베이비붐 세대 스포츠 팬을 대상으로 연구를 진행하였다. 빈도분석, 확인적 요인분석, 신뢰도 분석, 상관관계 분석을 실시하였으며, 가설을 검증하기 위해 경로분석이 진행되었다. 또한 매개효과 검증을 위해 부트스트랩 방법이 활용되었다. 결과: 그 결과, 첫째, 팀 동일시가 시청몰입에 긍정적 영향을 미쳤다. 둘째, 시청몰입은 사회적 고립감에 부정적 영향을 미쳤다. 셋째, 팀 동일시는 사회적 고립감에 부정적 영향을 미쳤다. 넷째, 시청몰입은 팀 동일시와 사회적 고립감의 관계에서 부분 매개하는 것으로 나타났다. 결론: 이와 같은 결과는 현재 베이비붐 세대가 사회적 거리두기로 겪고 있는 사회적 고립감 문제를 대면하지 않고도 해결할 수 있도록 돕고 있으며, 더불어 향후 또 다른 고립의 상황에 대비할 수 있는 자료를 제공하고 있다.

경주지진 및 포항지진을 통해 우리나라도 지진의 안전지대가 아니라는 인식이 증가하면서 지진피해를 줄일 수 있는 내진 설계에 대한 관심이 증가하고 있다. 본 논문에서는 내진설계 기술 중 현재까지 가장 효과적인 방법의 하나로 알려져 있는 면진구조에 대하여 검토하고자 한다. 이를 위해 중층 RC 건축물에 면진시스템을 적용한 후 경계비선형동적해석을 통해 비면진건축물과 면진건축물의 내진성능효과를 비교 분석하였다.

Shows the problem of exposure to vibration generated from vehicle and train adjacent to the railway facilities are housing facilities. As a method for solving the problems caused by the vibration countermeasures have been provided various methods such as the vibration of the vibration trajectory, wave barriers wall, vibration isolation mat. Therefore, in the present study is to evaluate the vibration analysis by using vibration isolation mat for vibration reduction of housing facilities.

Seismic isolation offers an attractive approach for reducing seismic loads in the building and in its components. This paper deals with the seismic isolation effects due to variation in period ratio between superstructure and isolation layer of the building. Seismic isolation effects of the moment-resisting frames with the various period ratio were studied by nonlinear time history analysis. It is recommended that the isolation period should be greater than 2.0 times of that of the superstructure for the effective seismic isolation.

Carbonic maceration 처리는 포도주 제조 시 사과산을 감소시키는 방법으로 사과산 감소의 원인 중 미생물의 영향을 알아보고자 사과산을 감소시키고 젖산을 생성시키는 미생물을 분리, 동정한 결과Lactobacillus brevis, Lactobacillus plantarum 및 Streptococcus thermophilus의 젖산균이 존재하는 것으로 나타났다. 분리된 균들은 대부분 당에서 젖산을 생성하는 균으로 알려져 있으며 사과산을

식용으로 사용되는 산채의 일종인 어수리의 지상부로부터 peroxynitrite 소거활성을 나타내는 성분을 분리한 결과 플라보노이드 성분인 hyperoside와 astragalin이 분리되었다. 특히, 높은 활성을 보였던 hyperoside는 이 식물재료 중 함량도 높아 어수리 잎은 peroxynitrite 과잉생산시 유발될 수 있는 당뇨병, 비만, 고지혈증, 심장질환 등의 예방과 치료에 유익한 식품이라 할 수 있을 것이다.