In animal development, the mechanisms by which localized factors and organelles in egg cytoplasm were exactly distributed into each daughter cell are essential for formation of various cell types. During ascidian Halocynthia roretzi embryogenesis, ooplasmic mitochondria were mainly segregated into muscle and neural precursor cells. At the 32-cell stage, localized mitochondria in the B6.2 blastomeres were preferentially distributed into the B7.4 muscle precursors compared with the B7.3 mesenchyme/ notochord precursors. When the B6.2 blastomeres were isolated from the early 32-cell stage embryos and then allowed to divide 2 times of cell division, the resultant partial embryos showed symmetric distribution of mitochondria, and the partial embryos were composed of equal size cells. In normal development, cell fates of the B7.3 blastomere were correlated with the unequal cleavage of B6.2 lineage cells that normally occurs in the next two-cell division stages to produce a large B8.5 mesenchyme and a small B8.6 notochord cell. Mitochondria are distributed asymmetrically in both cells. When embryos were treated with FGF receptor inhibitor SU5402 and MEK inhibitor U0126 between the 32-cell and the early 64-cell stages, the resultant embryos showed equal cleavage pattern and symmetric distribution of mitochondria in daughter cells of the B6.2 blastomeres. However, blocking of Nodal and Notch signaling did not affect the cell division pattern and mitochondrial distribution in the B6.2 lineage blastomeres between the 32-cell and 110-cell stages. Therefore, it is likely that FGF/MEK signaling is involved in asymmetric distribution of mitochondria and unequal cleavage of the B6.2 lineage blastomeres in ascidian embryo.

본 실증적 연구의 목적은 게임에서 게이머의 사회적 상호작용을 통해 발현되는 조직시민행동 이 게임의 지속 사용의도에 어떠한 영향을 미치는지 알아보고, 게이머의 이타적 행동과 순응적 행동 유도에 대한 학술적, 실무적 시사점을 제공하는 것이다. 이를 위해 조직시민행동 이론을 바탕으로 가설을 설정하였고, 리니지2 게이머 134명을 대상으로 실증 분석을 실시하였다. 분석 결과, 게이머의 조직시민행동인 이타적 행동과 순응적 행동은 게임의 지속 사용의도에 유의미 한 영향을 미치는 것으로 나타났다.